A new series of ferroelectric liquid crystals (FLCs) has been designed for active matrix displays... more A new series of ferroelectric liquid crystals (FLCs) has been designed for active matrix displays based upon the chiral smectic C phase. The FLCs have been derived from optically active fluorinated alkanols and a laterally fluoro substituted biphenylyl-1,3-dioxan core. Their physical properties such as spontaneous polarization, current response time, and tilt angle have been determined. The FLC derived from 2-fluoro-octanol showed a very short current response time of 10μs at T Sm*-N* - T= 10°C, while another FLC with the same core derived from 5-fluoro-octanol gave a value of 150μs.
Ferroelectric liquid crystals (FLCs) showing a short response time have been investigated with re... more Ferroelectric liquid crystals (FLCs) showing a short response time have been investigated with respect to their application for high resolution graphic displays addressed by thin film transistors. It is found that the introduction of fluoro substituents in the molecule at a suitable position such as the core and the asymmetric tail allowed the compounds to have a short response time. Here, in order to obtain further information about the fluorination effect onthestructure, a new series of ferroelectric liquid crystals having a 2-(biphenyl-4′-y)-1,3-dioxanyl group as the core has been synthesised. Their mesomorphic and physical properties have been measured, and the correlation between the structures and the properties is discussed.
The combination of surface-modified nanomaterials and chiral selectors can improve enantioselecti... more The combination of surface-modified nanomaterials and chiral selectors can improve enantioselective recognition with a significant influence on the performance of enantioselective detection, analysis, and separation.
IOP Conference Series: Earth and Environmental Science, 2022
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a virus that has caused the coron... more Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a virus that has caused the corona pandemic since 2019 or known as Covid-19. The cleavage of its polyprotein started this viral replication into functional viral proteins by two proteases: 3-chymotrypsin-like protease (3CL protease), also known as main protease (Mpro), and Papain-like protease (PLpro). Medicinal plant bioactive constituents could potentially become protease inhibitor agents of this virus and prevent viral replication. Thus, further might be developed into drug candidates for diseases with no specific drug currently available. The first step of discovering the medicine is virtual screening with a molecular docking simulation approach. The stable conformation structure of the bioactive compounds was docked into the enzymes SARS-CoV-2 Main Protease (PDB ID: 6XMK) and SARS-CoV-2 Papain-Like Protease (PDB ID: 7CMD). Molecular docking simulations were operated using Molegro Virtual Docker (MVD) program after ...
IOP Conference Series: Earth and Environmental Science, 2022
Coronavirus disease-19 (COVID-19) pandemic caused millions of deaths and socio-economics damage w... more Coronavirus disease-19 (COVID-19) pandemic caused millions of deaths and socio-economics damage worldwide. Corresponding to this, many studies on antiviral drugs exploration are rising to investigate the potential of drug compounds that can repress the replication of the SARS-CoV-2 virus by targeting its main protease named 3-chymotrypsin like protease (3CLpro). Without 3CLpro splicing, polyproteins of SARS-CoV-2 will not function to form new virions. We conducted an in silico thermodynamic study, and found several already known compounds from natural sources such as lovastatin, quinidine, and quinine were potential in inhibiting 3CLpro. However, most of the findings still need further wet-lab experiments to confirm their activity against it. To facilitate rapid screening of protease inhibitors, we aim to develop a screening kit for researchers with focus studies on herbal sources for COVID-19 treatments. Hence, the development of 3CLpro as the target of inhibition screening is esse...
IOP Conference Series: Earth and Environmental Science, 2022
The SARS-CoV-2 virus that caused pandemic COVID-19 has spread rapidly to humans and causes a very... more The SARS-CoV-2 virus that caused pandemic COVID-19 has spread rapidly to humans and causes a very serious health and social problem around the world. In order to develop vaccine and therapeutic approaches, a comprehensive study of viral structure and viral biological infection needs to be understood. In addition to four structural proteins; spike glycoprotein (S), envelope (E), membrane protein (M), and nucleocapsid protein (N), SARS-CoV-2 viral genome, consist of two open reading frame (ORF) which is located in N-terminus, ORF1a, and ORF1b. The ORF1a consist of Main protease (3CLpro) and together with papain-like protease (PLpro) play a role in the cleavage of polyproteins and translated from viral RNA to mature protein. Here we describe a method of production of 6xHis-tagged Papain-like protease fragment in E. coli RIPL, including gene expression, solubilization of inclusion bodies by different methods, and detection of gene product target by Western Blot Analyses.
Sunaryanto et al, 2019. Optimization of Penicillium lagena Medium Cultivation on Antifungal Patho... more Sunaryanto et al, 2019. Optimization of Penicillium lagena Medium Cultivation on Antifungal Pathogen of Phellinus lamaoensis using Response Surface Methodology. pp. 410-420. Phellinus lamaoensis (Murr.) Hein is fungal pathogen that can cause brown root rot disease in cocoa, tea, rubber, and coffee plants. Endophytic fungi, Penicillium lagena, isolated from bandotan (Ageratum conyzoides Linn.), medicinal plant, is able to inhibit the growth of pathogenic, P. lamaoensis. The effect of carbon source, nitrogen source, and mineral solution was studied. Lactose, yeast extract, and mineral solution were media components which showed significant effect toward production of P. lagena active compound. Composition optimization of these three medium components was done by response surface methodology (RSM). The Optimal response region of the significant factor was predicted by using a second order polynomial model with statistical design, central composite design (CCD). Higest production of P. ...
Scaffold is a 3-dimensional matrix created as a new bone cell growth medium made from natural pol... more Scaffold is a 3-dimensional matrix created as a new bone cell growth medium made from natural polymers and bioceramics. The extracted pectin from green Cincau leaves (Premna oblongifolia Merr) and hydroxyapatite (HA) are used in the manufacture of scaffolds. Pectin was extracted using citric acid with variation concentration of 0, 0.1, 0.2 and 0.3% (w / v). The 3% (w / w) HA-pectin mixture, dried freeze using a freeze dryer. The characterization of extracted pectin and HA-pectin scaffold was then performed. The results showed that pectin of green Cincau leaves had low methoxyl content, which was 1.364 to 5.022%. The resulting scaffold has a pore size ranging from 8.25 to 115 µm while the scaffold resistance to the load, ie 0.03 to 0.15 MPa. The scaffold porosity that has been made is 15.33 to 40.97% while the density is 0.69 to 1.02 g/cm3.
SYNTHESIS AND STRUCTURE CHARACTERIZATION OF ESTER BENZOATE TYPE OF FERROELECTRIC LIQUID CRYSTAL D... more SYNTHESIS AND STRUCTURE CHARACTERIZATION OF ESTER BENZOATE TYPE OF FERROELECTRIC LIQUID CRYSTAL DERIVED FROM (S)-(+)-2-METHYLBUTANOIC ACID. Synthesis method of ester benzoate type of ferroelectric liquid crystal derived from (S)-(+)-2-methylbutanoic acid, was developed via esterification between (S)-(+)-p-hydroxyphenyl-2-methylbutanoate ((S)-(2-HFM)) and acryloyloxy butyloxy benzoic acid (ABB). The chiral alcohol compound of (S)-(+)-2-HFM was obtained by Steglich selective-esterification of hydroquinone with dicyclohexylcarbodiimide (DCC) and dimethylaminopyridine (DMAP) as catalists. While acrylate compound of ABB was obtained by a serie of synthesis processes of 4-chlorobuthyl acetic acid as a main compund. The serie of processes started from a formation of Williamson ether, and then followed by a base hydrolisis, and finally ended by an acrylation process. Structure characterization of the obtained ferroelectric liquid crystal was carried out by Fourier Transform Infra Red (FT-IR...
Abstract: It was successfully synthesized liquid crystal monomer acrylate that conjugated with tw... more Abstract: It was successfully synthesized liquid crystal monomer acrylate that conjugated with two mesogens were cholesterol and p-Hydroxyphenyl-2-Methyl Butanoat which called MA (monomer cholesteryl acrylate) and monomer (S)-(+)-4-(2-Methyl butanoat-1-butyloxy)phenyl 4-[1-(propenoyloxy) butyloxy]benzoate (MB). Two monomers were characterization by Differential Scanning Calorimetry (DSC), Polarization Optical Microscopy (POM), X Ray Diffraction (XRD). Mesophase temperature of MA and MB are 81.28 o C and 54.36 o C. Textures analysis by POM showed MA was oily streak and MB was schlieren. XRD pattern show the strongest 3 peaks of MA at room temperature are (2Theta, deg): 2.7153; 5.2992 and 18.8500. Strongest 3 peaks of MB at room temperature are (2Theta, deg): 9.1726; 9.7707 and 12.5389. XRD pattern MA and MB at mesophase and above mesophase temperature that each peaks disappear. Key words: Liquid crystal, cholesteryl acrylate, p-hydroxyphenyl-2-methylbutanoat.
Synthesized cellulosic palmitat has been used as thin solid membranes for enantioseparation of ib... more Synthesized cellulosic palmitat has been used as thin solid membranes for enantioseparation of ibuprofen (NSAID). Two kinds of solid membranes have been manufactured those are consisted of a pure of cellulosic palmitat and of a mixture of cellulosic palmitat and liquid crystal material. The membranes have been utilized to understand about the enantiomeric separation performance at ambient conditions using methanol or acetonitrile as eluent. Identification of ibuprofen was successfully determined using high performance liquid chromatography equipped with OD-RH reverse coloumn using acetonitrile-buffer pH4 as mobile phase (&alpha
Ramie fiber is a potential raw material to substitute imported raw materials such as cotton. Due ... more Ramie fiber is a potential raw material to substitute imported raw materials such as cotton. Due to its higher hemicellulose content, ramie fiber required hydrolysis in a process called degumming. Enzymatic degumming is environmentally friendly compared to traditional process which using chemicals. Alkalithermophilic xylanase have high ability in hemicellulose hydrolysis. The production of xylanase was conducted by submerged fermentation of Bacillus halodurans CM1 in 20L bioreactor using Mamo and corncob medium with optimum conditions at 50°C, pH 9, 150 RPM and 1 vvm. The optimum specific activity of xylanase measured by Bailey method at 70°C and pH 9 is 475.41 U/mg. Xylanase was stable at 50°C, pH 9 and relatively stable to K+, Na2+, Co2+ and Ca2+ metal ions and Triton-X, Saba dan Tween-80 surfactants. Degumming process was carried out by immersing ramie fibers in formulated degumming solution with vlot 1:20 at 50°C, 150 RPM and 180 minutes. The enzymatic degumming process may subs...
A new series of ferroelectric liquid crystals (FLCs) has been designed for active matrix displays... more A new series of ferroelectric liquid crystals (FLCs) has been designed for active matrix displays based upon the chiral smectic C phase. The FLCs have been derived from optically active fluorinated alkanols and a laterally fluoro substituted biphenylyl-1,3-dioxan core. Their physical properties such as spontaneous polarization, current response time, and tilt angle have been determined. The FLC derived from 2-fluoro-octanol showed a very short current response time of 10μs at T Sm*-N* - T= 10°C, while another FLC with the same core derived from 5-fluoro-octanol gave a value of 150μs.
Ferroelectric liquid crystals (FLCs) showing a short response time have been investigated with re... more Ferroelectric liquid crystals (FLCs) showing a short response time have been investigated with respect to their application for high resolution graphic displays addressed by thin film transistors. It is found that the introduction of fluoro substituents in the molecule at a suitable position such as the core and the asymmetric tail allowed the compounds to have a short response time. Here, in order to obtain further information about the fluorination effect onthestructure, a new series of ferroelectric liquid crystals having a 2-(biphenyl-4′-y)-1,3-dioxanyl group as the core has been synthesised. Their mesomorphic and physical properties have been measured, and the correlation between the structures and the properties is discussed.
The combination of surface-modified nanomaterials and chiral selectors can improve enantioselecti... more The combination of surface-modified nanomaterials and chiral selectors can improve enantioselective recognition with a significant influence on the performance of enantioselective detection, analysis, and separation.
IOP Conference Series: Earth and Environmental Science, 2022
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a virus that has caused the coron... more Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a virus that has caused the corona pandemic since 2019 or known as Covid-19. The cleavage of its polyprotein started this viral replication into functional viral proteins by two proteases: 3-chymotrypsin-like protease (3CL protease), also known as main protease (Mpro), and Papain-like protease (PLpro). Medicinal plant bioactive constituents could potentially become protease inhibitor agents of this virus and prevent viral replication. Thus, further might be developed into drug candidates for diseases with no specific drug currently available. The first step of discovering the medicine is virtual screening with a molecular docking simulation approach. The stable conformation structure of the bioactive compounds was docked into the enzymes SARS-CoV-2 Main Protease (PDB ID: 6XMK) and SARS-CoV-2 Papain-Like Protease (PDB ID: 7CMD). Molecular docking simulations were operated using Molegro Virtual Docker (MVD) program after ...
IOP Conference Series: Earth and Environmental Science, 2022
Coronavirus disease-19 (COVID-19) pandemic caused millions of deaths and socio-economics damage w... more Coronavirus disease-19 (COVID-19) pandemic caused millions of deaths and socio-economics damage worldwide. Corresponding to this, many studies on antiviral drugs exploration are rising to investigate the potential of drug compounds that can repress the replication of the SARS-CoV-2 virus by targeting its main protease named 3-chymotrypsin like protease (3CLpro). Without 3CLpro splicing, polyproteins of SARS-CoV-2 will not function to form new virions. We conducted an in silico thermodynamic study, and found several already known compounds from natural sources such as lovastatin, quinidine, and quinine were potential in inhibiting 3CLpro. However, most of the findings still need further wet-lab experiments to confirm their activity against it. To facilitate rapid screening of protease inhibitors, we aim to develop a screening kit for researchers with focus studies on herbal sources for COVID-19 treatments. Hence, the development of 3CLpro as the target of inhibition screening is esse...
IOP Conference Series: Earth and Environmental Science, 2022
The SARS-CoV-2 virus that caused pandemic COVID-19 has spread rapidly to humans and causes a very... more The SARS-CoV-2 virus that caused pandemic COVID-19 has spread rapidly to humans and causes a very serious health and social problem around the world. In order to develop vaccine and therapeutic approaches, a comprehensive study of viral structure and viral biological infection needs to be understood. In addition to four structural proteins; spike glycoprotein (S), envelope (E), membrane protein (M), and nucleocapsid protein (N), SARS-CoV-2 viral genome, consist of two open reading frame (ORF) which is located in N-terminus, ORF1a, and ORF1b. The ORF1a consist of Main protease (3CLpro) and together with papain-like protease (PLpro) play a role in the cleavage of polyproteins and translated from viral RNA to mature protein. Here we describe a method of production of 6xHis-tagged Papain-like protease fragment in E. coli RIPL, including gene expression, solubilization of inclusion bodies by different methods, and detection of gene product target by Western Blot Analyses.
Sunaryanto et al, 2019. Optimization of Penicillium lagena Medium Cultivation on Antifungal Patho... more Sunaryanto et al, 2019. Optimization of Penicillium lagena Medium Cultivation on Antifungal Pathogen of Phellinus lamaoensis using Response Surface Methodology. pp. 410-420. Phellinus lamaoensis (Murr.) Hein is fungal pathogen that can cause brown root rot disease in cocoa, tea, rubber, and coffee plants. Endophytic fungi, Penicillium lagena, isolated from bandotan (Ageratum conyzoides Linn.), medicinal plant, is able to inhibit the growth of pathogenic, P. lamaoensis. The effect of carbon source, nitrogen source, and mineral solution was studied. Lactose, yeast extract, and mineral solution were media components which showed significant effect toward production of P. lagena active compound. Composition optimization of these three medium components was done by response surface methodology (RSM). The Optimal response region of the significant factor was predicted by using a second order polynomial model with statistical design, central composite design (CCD). Higest production of P. ...
Scaffold is a 3-dimensional matrix created as a new bone cell growth medium made from natural pol... more Scaffold is a 3-dimensional matrix created as a new bone cell growth medium made from natural polymers and bioceramics. The extracted pectin from green Cincau leaves (Premna oblongifolia Merr) and hydroxyapatite (HA) are used in the manufacture of scaffolds. Pectin was extracted using citric acid with variation concentration of 0, 0.1, 0.2 and 0.3% (w / v). The 3% (w / w) HA-pectin mixture, dried freeze using a freeze dryer. The characterization of extracted pectin and HA-pectin scaffold was then performed. The results showed that pectin of green Cincau leaves had low methoxyl content, which was 1.364 to 5.022%. The resulting scaffold has a pore size ranging from 8.25 to 115 µm while the scaffold resistance to the load, ie 0.03 to 0.15 MPa. The scaffold porosity that has been made is 15.33 to 40.97% while the density is 0.69 to 1.02 g/cm3.
SYNTHESIS AND STRUCTURE CHARACTERIZATION OF ESTER BENZOATE TYPE OF FERROELECTRIC LIQUID CRYSTAL D... more SYNTHESIS AND STRUCTURE CHARACTERIZATION OF ESTER BENZOATE TYPE OF FERROELECTRIC LIQUID CRYSTAL DERIVED FROM (S)-(+)-2-METHYLBUTANOIC ACID. Synthesis method of ester benzoate type of ferroelectric liquid crystal derived from (S)-(+)-2-methylbutanoic acid, was developed via esterification between (S)-(+)-p-hydroxyphenyl-2-methylbutanoate ((S)-(2-HFM)) and acryloyloxy butyloxy benzoic acid (ABB). The chiral alcohol compound of (S)-(+)-2-HFM was obtained by Steglich selective-esterification of hydroquinone with dicyclohexylcarbodiimide (DCC) and dimethylaminopyridine (DMAP) as catalists. While acrylate compound of ABB was obtained by a serie of synthesis processes of 4-chlorobuthyl acetic acid as a main compund. The serie of processes started from a formation of Williamson ether, and then followed by a base hydrolisis, and finally ended by an acrylation process. Structure characterization of the obtained ferroelectric liquid crystal was carried out by Fourier Transform Infra Red (FT-IR...
Abstract: It was successfully synthesized liquid crystal monomer acrylate that conjugated with tw... more Abstract: It was successfully synthesized liquid crystal monomer acrylate that conjugated with two mesogens were cholesterol and p-Hydroxyphenyl-2-Methyl Butanoat which called MA (monomer cholesteryl acrylate) and monomer (S)-(+)-4-(2-Methyl butanoat-1-butyloxy)phenyl 4-[1-(propenoyloxy) butyloxy]benzoate (MB). Two monomers were characterization by Differential Scanning Calorimetry (DSC), Polarization Optical Microscopy (POM), X Ray Diffraction (XRD). Mesophase temperature of MA and MB are 81.28 o C and 54.36 o C. Textures analysis by POM showed MA was oily streak and MB was schlieren. XRD pattern show the strongest 3 peaks of MA at room temperature are (2Theta, deg): 2.7153; 5.2992 and 18.8500. Strongest 3 peaks of MB at room temperature are (2Theta, deg): 9.1726; 9.7707 and 12.5389. XRD pattern MA and MB at mesophase and above mesophase temperature that each peaks disappear. Key words: Liquid crystal, cholesteryl acrylate, p-hydroxyphenyl-2-methylbutanoat.
Synthesized cellulosic palmitat has been used as thin solid membranes for enantioseparation of ib... more Synthesized cellulosic palmitat has been used as thin solid membranes for enantioseparation of ibuprofen (NSAID). Two kinds of solid membranes have been manufactured those are consisted of a pure of cellulosic palmitat and of a mixture of cellulosic palmitat and liquid crystal material. The membranes have been utilized to understand about the enantiomeric separation performance at ambient conditions using methanol or acetonitrile as eluent. Identification of ibuprofen was successfully determined using high performance liquid chromatography equipped with OD-RH reverse coloumn using acetonitrile-buffer pH4 as mobile phase (&alpha
Ramie fiber is a potential raw material to substitute imported raw materials such as cotton. Due ... more Ramie fiber is a potential raw material to substitute imported raw materials such as cotton. Due to its higher hemicellulose content, ramie fiber required hydrolysis in a process called degumming. Enzymatic degumming is environmentally friendly compared to traditional process which using chemicals. Alkalithermophilic xylanase have high ability in hemicellulose hydrolysis. The production of xylanase was conducted by submerged fermentation of Bacillus halodurans CM1 in 20L bioreactor using Mamo and corncob medium with optimum conditions at 50°C, pH 9, 150 RPM and 1 vvm. The optimum specific activity of xylanase measured by Bailey method at 70°C and pH 9 is 475.41 U/mg. Xylanase was stable at 50°C, pH 9 and relatively stable to K+, Na2+, Co2+ and Ca2+ metal ions and Triton-X, Saba dan Tween-80 surfactants. Degumming process was carried out by immersing ramie fibers in formulated degumming solution with vlot 1:20 at 50°C, 150 RPM and 180 minutes. The enzymatic degumming process may subs...
Uploads
Papers by Asep Riswoko