Papers by Paweł Maćkowiak
Acta Biochimica Polonica, 1993
Archivum veterinarium Polonicum / Polish Academy of Sciences, Committee of Veterinary Sciences, 1992
The purpose of the presented study is a comparison of phytooestrogen--coumestrol and oestrone eff... more The purpose of the presented study is a comparison of phytooestrogen--coumestrol and oestrone effects on carbohydrate metabolism in ovariectomized female rats and to examine the participation of pancreatic hormones in changes of this metabolism. Administration of coumestrol diminished muscle glycogen in investigated animals. There were no significant changes in insulin and glucagon blood level but decrease in the specific insulin binding in the muscle membranes was observed. It suggests that coumestrol effects the insulin receptor activity in this tissue and it could be a cause of glycogen deficiency.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 1993
D,L-arginine and L-lysine, introduced into the alimentary canal (IAC), caused significant secreti... more D,L-arginine and L-lysine, introduced into the alimentary canal (IAC), caused significant secretion of insulin in rabbits, whereas D,L-methionine or L-phenylalanine evoked only a small effect. Also, intravenous (IV) injection of D,L-arginine caused dose dependent and biphasic insulin output. On the other hand, L-phenylalanine given IV decreased both basal and glucose--stimulated insulin level in blood.
Molecular medicine reports
In the present study, the influence of chromium(III) complexes (acetate, chloride, glycinate, his... more In the present study, the influence of chromium(III) complexes (acetate, chloride, glycinate, histidinate, lactate and propionate) on insulin binding and signal transduction [phosphorylation of tyrosine and serine in the insulin receptor substrate (IRS)-1] was investigated in vitro using three experimental models: isolated rat liver membranes and cultured mouse C2C12 myoblasts or 3T3-L1 preadipocytes. The examined complexes did not elevate the binding of insulin to the liver membranes. Moreover, chromium histidinate, lactate, acetate and propionate complexes diminished the specific binding of insulin. Simultaneously, chromium chloride, which did not significantly elevate insulin binding, increased the number of membrane accessible particles of the insulin receptors. However, it was accompanied by slightly diminished affinity of the receptor to the hormone. Chromium acetate and propionate significantly diminished the binding capacity of the low-affinity insulin receptor class. Invest...
Naturwissenschaften, 1992
... GKSS-For-schungszentrums Geesthacht 2. Abu E1-Ela, A., Hassan, S., Bagge, E. R.: Atomkernener... more ... GKSS-For-schungszentrums Geesthacht 2. Abu E1-Ela, A., Hassan, S., Bagge, E. R.: Atomkernenergie/Kerntechnik 47, 109 (1985) 3. Bagge, ER : Welt ... 63, 93 (1984) 6. DOhler, KD: Experientia 42, 788 (1986) 7. Gambhir, KK, Archer, JA, Bradley, CJ : Diabetes 27, 701 (1978) ...
Archives Of Physiology And Biochemistry, 2001
Islets, 2016
Resistin participates in the regulation of energy homeostasis, insulin resistance, and inflammati... more Resistin participates in the regulation of energy homeostasis, insulin resistance, and inflammation. The potential expression in pancreas, and modulation of the endocrine pancreas secretion by resistin is not well characterized, therefore, we examined it on several levels. We examined the localization of resistin in rat pancreatic islets by immunohistochemistry and immunofluorescence, and the potential presence of resistin mRNA by RT-PCR and protein by Western Blot in these structures. In addition, we studied the regulation of insulin and glucagon secretion by resistin in pancreatic INS-1E band InR-G9 a-cell lines as well as isolated rat pancreatic islets. We identified resistin immunoreactivity in the periphery of rat pancreatic islets and confirmed the expression of resistin at mRNA and protein level. Obtained data indicated that resistin is co-localized with glucagon in pancreatic a-cells. In addition, we found that in vitro resistin decreased insulin secretion from INS-1E cells and pancreatic islets at normal (6 mM) and high (24 mM) glucose concentrations, and also decreased glucagon secretion from G9 cells and pancreatic islets at 1 mM, whereas a stimulation of glucagon secretion was observed at 6 mM glucose. Our results suggest that resistin can modulate the secretion of insulin and glucagon from clonal b or a cells, and from pancreatic islets.
The experiments were carried out on ovariectomized Wistar rats Their livers were perhsed with lba... more The experiments were carried out on ovariectomized Wistar rats Their livers were perhsed with lbasic perhsion medium (BPM) or BPM supplemented with isoflavone genistein. insulin or combination of the two factors. The obtained results support the hypothesis that genistein influences the kinetics of insulin binding to cell membranes changing the number of insulin receptors and dissociation constant (Kd) BPM supplementation with genisl.eiii decreased number of high affinity insulin receptors (HAIR) both in livers ti-eat:ed and untreated with insulin. The amount of HA1R diminished significantly froin 6101t77 x lo-'' (no genistein) to 2333+72 x lo-'' niol/mg of membrane protein (supplement of genistein). Similarly. genistein reduced slightly the amount of HAIR even \vhen added together with: inslulin (372259 x lo-" mol/nig) in comparison to rats perhsed with medium containing insulin but not the isoflavone (42146 x 10-" mol/mg). Simultaneously, genistein decreas...
Acta Biochimica Polonica, 1993
The comparative studies were performed on insulin receptors of erythrocytes and liver plasma memb... more The comparative studies were performed on insulin receptors of erythrocytes and liver plasma membranes in fish (tench and carp) and bird (duck). The Scatchard plots indicated the presence of two pools of binding sites both in fish and duck. These pools show inter-species differences in binding ability and the number of receptors. Specific binding of insulin and the binding affinity are higher in duck than in fish.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 1998
The first step in insulin action is its specific binding to alpha-subunits of the receptor in the... more The first step in insulin action is its specific binding to alpha-subunits of the receptor in the plasma membrane. Rats of Wistar strain are commonly used as laboratory animals but there are no data comparing insulin binding by various rat tissues. We studied the insulin binding kinetics in plasma membranes isolated from hearts, livers, brains, skeletal muscles, adipose tissue, thymus and testes in order to compare their ability to bind 125I-insulin and to test which membrane preparation is most useful and convenient for such a study. The dissociation constant (Kd) and the quantity of receptors measured as a binding capacity were determined by the Scatchard method using the LIGAND computer program whereas the binding potency of insulin was calculated as IC50 using the ALLFIT computer program. We also introduced the product of Ka x R50 (affinity constant multiplied by binding capacity) as an index which describes the functional features of insulin receptors taking into account both n...
Physiological Research, 2018
Spexin (SPX) and kisspeptin (KISS) are novel peptides relevant in the context of regulation of me... more Spexin (SPX) and kisspeptin (KISS) are novel peptides relevant in the context of regulation of metabolism, food intake, puberty and reproduction. Here, we studied changes of serum SPX and KISS levels in female non-obese volunteers (BMI<25 kg/m2) and obese patients (BMI>35 kg/m2). Correlations between SPX or KISS with BMI, McAuley index, QUICKI, HOMA IR, serum levels of insulin, glucagon, leptin, adiponectin, orexin-A, obestatin, ghrelin and GLP-1 were assessed. Obese patients had lower SPX and KISS levels as compared to non-obese volunteers (SPX: 4.48±0.19 ng/ml vs. 6.63±0.29 ng/ml; p<0.001, KISS: 1.357±0.15 nmol/l vs. 2.165±0.174 nmol/l; p<0.01). SPX negatively correlated with BMI, HOMA-IR, insulin, glucagon, active ghrelin and leptin. Positive correlations were found between SPX and QUICKI index, McAuley index, serum levels of obestatin, GLP-1 and adiponectin and orexin-A Serum KISS negatively correlated with BMI, HOMA-IR, serum levels of insulin, glucagon, active ghre...
Journal of Animal and Feed Sciences, 2018
Journal of Animal and Feed Sciences, 2011
Two 42-d floor pen studies of identical design were conducted to evaluate the efficacy of enzyme ... more Two 42-d floor pen studies of identical design were conducted to evaluate the efficacy of enzyme supplementation of broiler diets containing full-fat double zero rapeseed. In both trials, 300 1-d-old Ross 308 males were allocated randomly to two dietary treatments with 15 replicates (10 birds per replicate). The birds received two isonutritive diets, pelleted at 78°C. Diets were either not supplemented or supplemented with a commercial enzyme blend providing xylanase and p-glucanase. In general, in both trials the supplementation of carbohydrase improved bird performance, however, only in the second experiment the overall body weight gain and feed conversion ratio were significantly improved. The enzyme addition reduced (P<0.05) the concentration of serum insulin (0.75 vs 0.49 ng/ml), total cholesterol (4.35 vs 3.03 mmol/1) and its fractions, whereas no effect on the concentration of blood glucose was observed. No significant effect of the enzymes on the ileal digestibility of nutrients or on apparent ileal metabohzable energy was found; however, total tract fat digestibility and AMEn value tended to be higher following enzyme supplementation. (78.2 vs 73.8% and 12.6 vs 12.4 MJ/kg). The results of the present study suggest that carbohydrases supplementation may improve the nutritive value of full-fat rapeseed in broiler chicken diets.
Polish Journal of Veterinary Sciences, 2011
Annals of Animal Science, 2014
The aim of the review is to present mechanisms of digestion in horses through a functional descri... more The aim of the review is to present mechanisms of digestion in horses through a functional description of the digestive system’s structure with emphasis on nutrient digestibility. In the era of an increasingly intensive and specialised sport usage of horses, also the significance of balancing their dietary nutrient value in accordance with individual requirements of these animals is gaining in importance. At the same time, it is not possible to meet nutritional requirements of a sport horse without knowledge about feed utilisation. In many farm animal species, digestibility is measured postmortem or via complicated and expensive cannulation of the digestive system. In horses, these methods are not applied due to ethical approaches or other limitations (e.g. sport horses); therefore, the importance of marker-based techniques is growing, although not much data is published in the available literature (Sales, 2012). Moreover, in contrast to other non-ruminants, horses are naturally ada...
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 2000
Aging is associated with insulin resistance but the exact molecular mechanism is still unknown. T... more Aging is associated with insulin resistance but the exact molecular mechanism is still unknown. Tissue insulin resistance can be evoked by the decreased sensitivity to insulin, the decreased responsiveness to hormone or both. As the first step in insulin action is its binding to alfa subunits of the receptor we, therefore, studied the insulin binding kinetics in plasma membranes of the liver, heart and skeletal muscle in order to establish whether their ability to bind the hormone is altered with aging. Plasma membranes were prepared and purified according to Havrankowa and binding assay was performed using (125I)-iodoinsulin. The kinetic parameters of the hormone-receptor interaction were analysed by the method of Scatchard using the LIGAND-Pc v.3.1. computer program. The binding potency of insulin was calculated as IC50 using ALLFIT-Pc v.2.7. computer program. We have shown that there are striking differences in insulin binding kinetics in newborn and old rats, depending on kind o...
International Journal of Molecular Medicine, 2005
3. Characterization of somatostatin receptor subtype-specific regulation of insulin and glucagon ... more 3. Characterization of somatostatin receptor subtype-specific regulation of insulin and glucagon secretion: an in vitro study on isolated human pancreatic islets. Singh V,
Annals of agricultural and environmental medicine : AAEM, 2011
Atherosclerotic vascular disease is currently the biggest threat and the highest cause of death w... more Atherosclerotic vascular disease is currently the biggest threat and the highest cause of death worldwide, approaching almost 60%.The development of atherosclerosis is affected by ecological factors associated with industry and pollution of the environment. Neurotensin (NT) is a peptide acting via 3 kinds of neurotensin receptors (NTR) localized in target tissues. In several studies, the presence of its receptors has been shown in chicken liver, and the influence of NT on the metabolism of this organ was confirmed (glycogenolysis stimulation through sympathetic nervous system, enterohepatic circulation of bile acids, metabolism of lipoproteins). Healthy male WISTAR rats weighing 300}30 grams, were used for the experiments. The animals were divided into 4 groups: 1) control group, to which 0.9% NaCl was administrated (i.p.); 2) the second group was given levocabastine 1mg/kg i.p.; 3) the third group received SR 48692 0.4 mg/; 4) the fourth group was given NT analog [D-Trp 11]-neurote...
Poultry Science, 2013
A total of 1,200 Ross broiler chickens were used in 2 separate feeding studies to explore the eff... more A total of 1,200 Ross broiler chickens were used in 2 separate feeding studies to explore the effect of myo-inositol (MYO) and phytase on performance and blood biochemistry of broilers fed diets formulated to be either adequate or insufficient in Ca and digestible P (dP). Supplementation of diets that were formulated to be insufficient in Ca and dP with MYO resulted in improved BW gain and feed conversion ratio in both experiments. However, these effects were most pronounced in the finisher phase, and moderate negative effects were observed during the starter period. Supplementation of the diet with microbial phytase improved BW gain and feed conversion ratio to a similar extent as was observed with MYO, and there was a degree of subadditivity between the 2 additives. Blood glucose concentrations were increased by both MYO and phytase, though possibly by different mechanisms, because insulin concentrations were not directly relatable to circulating glucose levels, especially when both MYO and phytase were applied simultaneously. The increase in blood glucose concentrations with MYO and phytase was most pronounced in the diet with a lower Ca and dP concentration. It can be concluded that dietary supplementation with MYO or phytase was effective in improving performance of commercial broiler chickens. However, further work is required to explore complex ontogenetic effects of MYO and possible involvement of both MYO and phytase in Na-dependent transport mechanisms.
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Papers by Paweł Maćkowiak