Papers by Luiz Fernando Ribeiro
Datasets - Sistema SALVE - ICMBio, Jun 6, 2023
Datasets - Sistema SALVE - ICMBio
Brachycephalus brunneus é endêmica do Brasil, conhecida para aa localidade-tipo, no Pico Caratuva... more Brachycephalus brunneus é endêmica do Brasil, conhecida para aa localidade-tipo, no Pico Caratuva, município de Campina Grande do Sul, e municípios vizinhos, no estado do Paraná. A espécie habita camadas espessas de serapilheira de floresta ombrófila densa altamontana primária do bioma Mata Atlântica, em altitudes entre 1.000 e 1.600 metros. Embora tenha distribuição bastante restrita, trata-se de espécie abundante, que ocorre em área preservada, não havendo ameaças evidentes que possam colocá-la em risco de extinção. Por isso, Brachycephalus brunneus foi avaliada como Menos Preocupante (LC).
Desejo agradecer a todos aqueles que de modo direto ou indireto contribuíram para a realização de... more Desejo agradecer a todos aqueles que de modo direto ou indireto contribuíram para a realização deste trabalho. À Dailey Fischer, por todo companheirismo, incentivo, amparo e contribuição dispensados durante todo o desenvolvimento desse trabalho sem o qual não teria sido concluído. Ao Prof. Sergio Furtado dos Reis, pela orientação no decorrer do planejamento, execução e redação deste trabalho.
Herpetologica, 2006
Two new species of brachycephalid frogs are described from Pico Marumbi, municipality of Morretes... more Two new species of brachycephalid frogs are described from Pico Marumbi, municipality of Morretes, and Pico da Igreja, municipality of Guaratuba, Paraná State, southern Brazil. The new species share the following attributes: body bufoniform; skin on top of the head, and central part of the back body smooth with no dermal co-ossification; outer metatarsal tubercle distinct; dermal roofing bones of skull unornamented; all paired cranial bones distinct and not fused; quadratojugals, and maxillary odontoids present. The new species from Pico Marumbi is characterized by male SVL 5 11.6-12.5 and female SVL 5 13.0-14.5 mm; and general color orange with dorsal reddish-brown irregular markings, lateral surfaces with small dark brown spots, and belly with brownish spots and small dots. The new species from Pico da Igreja is characterized by male SVL 5 12.6-13.9 and female SVL 5 14.6-15.3 mm; and general color orange, lateral surfaces with small dark brown spots, and belly with brownish coalescent spots and small dots. Comparisons with other brachycephalid species and osteological data are provided.
The advent of next-generation sequencing allows researchers to use large-scale datasets for speci... more The advent of next-generation sequencing allows researchers to use large-scale datasets for species delimitation analyses, yet one can envision an inflection point where the added accuracy of including more loci does not offset the increased computational burden. One alternative to including all loci could be to prioritize the analysis of loci for which there is an expectation of high informativeness, such as those with higher numbers of parsimonyinformative sites. Here, we explore the issue of species delimitation and locus selection with species from two anuran genera: Melanophryniscus (Bufonidae) and Brachycephalus (Brachycephalidae). Montane species in these genera have been isolated in sky islands across the southern Brazilian Atlantic Forest, which led to the formation of a number of microendemic species. To delimit species, we obtained genetic data using target enrichment of ultraconserved elements from 32 populations (13 for Melanophryniscus and 19 for Brachycephalus), and we were able to create datasets that included over 800 loci with no missing data. We ranked loci according to their corresponding number of parsimony-informative sites, and we performed species delimitation analyses using BPP in each genus based on the top 10, 20, 40, 80, 160, 320, and 640 loci. We also conducted several additional analyses using 10 randomly sampled datasets containing the same numbers of loci to discriminate the relative contribution of increasing the number of loci from prioritizing those with higher informativeness. We identified three types of node: nodes with either consistently high or low support regardless of the number of loci or their informativeness, and nodes that were initially poorly supported, but their support became stronger with more data. Adding more loci had a stronger impact on model support than prioritizing loci for their informativeness, but this effect was less apparent in datasets with more than 160 loci. When viewed across all sensitivity analyses, our results suggest that the current species richness in both genera might have been underestimated. In addition, our results provide .
Brachycephalus are among the smallest terrestrial vertebrates in the world. The genus encompasses... more Brachycephalus are among the smallest terrestrial vertebrates in the world. The genus encompasses 34 species endemic to the Brazilian Atlantic Rainforest, occurring mostly in montane forests, with many species showing microendemic distributions to single mountaintops. It includes diurnal species living in the leaf litter and calling during the day, mainly during the warmer months of the year. The ecology of the vast majority of the species is unknown, particularly their advertisement call (AC), which has been described only for seven species of the genus. In the present study, we describe the AC of Brachycephalus albolineatus, a recently described microendemic species from Santa Catarina, southern Brazil. We analyzed 34 advertisement calls (ACs) and 554 notes from 20 individuals of B. albolineatus, recorded between 5-6 February 2016 in the type locality of the species. We collected five individuals as vouchers (they are from the type series of the species). Eight individuals we recorded 2-4 times. Brachycephalus albolineatus have a long advertisement call of 40-191 s (mean of 88 s) composed of 7-26 notes (mean of 14 notes) emitted at a rate of 6-13 notes per minute (mean of 9 notes per minute) and at a dominant frequency of 5-7 kHz (mean of 6 kHz). Advertisement calls are composed of isolated notes and note groups (two notes involved in each particular note group); the former is composed by one to three pulses and the note groups by two or three pulses in each note. Most ACs present both isolated notes and note groups, with a few cases showing only the former. Note groups are emitted invariably in the last third of the AC. Most isolated notes escalate their number of pulses along the AC (1 to 2, 1 to 3 or 2 to 3). Note duration of isolated notes varies from 0.002-0.037 s (mean of 0.020 s) and duration of note group vary from 0.360-0.578 s (mean of 0.465 s). We show that there is intraindividual variation in several parameters [(1) duration and rate of the AC; (2) number of
Background. Brachycephalus includes miniaturized frogs with restricted geographical distributions... more Background. Brachycephalus includes miniaturized frogs with restricted geographical distributions throughout the Brazilian Atlantic Forest. Ecological data for most species are still scarce. For instance, advertisement calls have only been described for 12 of the 36 known species, including B. tridactylus, a recently described species from southern Brazil. Posteriorly, features of the advertisement call of B. tridactylus were compared with congeners and the unique characteristics of its call were highlighted. To confirm these potentially divergent characteristics, we reanalysed an original recording of B. tridactylus and analysed our own recordings and verified that the original description of its advertisement call is inaccurate. Thus, we redescribe its advertisement calls. Methods. We asked the descriptors of B. tridactylus the original recordings that they made and requested access to the only original recording deposited in a collection of sounds. We received from André Lima a copy of one recording, the same as the one that had deposited, and obtained permission to re-analyze it. We studied this recording and compared it with our own recordings, made at the type locality of the species on March, 2016. Sound samples were analysed with Raven Pro 1.5.0 and call analyses were made under a note-centered approach. Results. The original recording was amplified somehow by at least 6 dB and was also clearly low-pass filtered with a cutoff frequency of 10 kHz. Our analyses did not allow us to recognize several of the acoustic parameters normally described in Brachycephalus. The sound we heard from the notes overlapped with other signals (noise?), which prevented us from clearly determining the end of the note and other important features, such as the presence of pulses. According to our recordings (n = 15 individuals), B. tridactylus emitted a relatively long advertisement call (50.8 s, on average), composed by 10-13 notes emitted in a note rate of 3.7-8.3 notes per minute. Only isolated notes were present. The notes were composed by 1-3 pulses and the note duration varied from 0.002-0.021 s. Discussion. The original description of the call of B. tridactylus is incorrect because it included background noise and amplification artefacts as part of the call parameters. However, we recognize that the original recording and our recordings have captured the same type of call. In our measurements of the species calls, note duration was nearly an order of magnitude shorter as the original description. The existence of notes with 1-3 pulses was not acknowledged in the original description. With few pulses per notes, the advertisement call of B. tridactylus is distinct from the notes with several pulses of B. ephippium, B. pitanga, B. crispus, B. sulfuratus, and B. darkside. The advertisement calls of B. tridactylus is also distinct from that of B. albolineatus and B. mirissimus by having only isolates notes, instead of
Molecular Phylogenetics and Evolution, Dec 1, 2019
The advent of next-generation sequencing allows researchers to use large-scale datasets for speci... more The advent of next-generation sequencing allows researchers to use large-scale datasets for species delimitation analyses, yet one can envision an inflection point where the added accuracy of including more loci does not offset the increased computational burden. One alternative to including all loci could be to prioritize the analysis of loci for which there is an expectation of high informativeness, such as those with higher numbers of parsimonyinformative sites. Here, we explore the issue of species delimitation and locus selection with species from two anuran genera: Melanophryniscus (Bufonidae) and Brachycephalus (Brachycephalidae). Montane species in these genera have been isolated in sky islands across the southern Brazilian Atlantic Forest, which led to the formation of a number of microendemic species. To delimit species, we obtained genetic data using target enrichment of ultraconserved elements from 32 populations (13 for Melanophryniscus and 19 for Brachycephalus), and we were able to create datasets that included over 800 loci with no missing data. We ranked loci according to their corresponding number of parsimony-informative sites, and we performed species delimitation analyses using BPP in each genus based on the top 10, 20, 40, 80, 160, 320, and 640 loci. We also conducted several additional analyses using 10 randomly sampled datasets containing the same numbers of loci to discriminate the relative contribution of increasing the number of loci from prioritizing those with higher informativeness. We identified three types of node: nodes with either consistently high or low support regardless of the number of loci or their informativeness, and nodes that were initially poorly supported, but their support became stronger with more data. Adding more loci had a stronger impact on model support than prioritizing loci for their informativeness, but this effect was less apparent in datasets with more than 160 loci. When viewed across all sensitivity analyses, our results suggest that the current species richness in both genera might have been underestimated. In addition, our results provide .
Journal of Zoology, May 24, 2022
The mineralized dermal layer (MDL) is found in most terrestrial anurans. Its thickness represents... more The mineralized dermal layer (MDL) is found in most terrestrial anurans. Its thickness represents on average up to 8% of that of the entire skin. It has been proposed that it may reduce body water loss, act on homeostasis, support skin structure, or conversely, it may be a currently functionless trait constrained by groups' evolutionary history. We described the MDL morphology of 11 Brachycephalus species, terrestrial, miniaturized and microendemic anurans, and tested for its relationship with climate of higher latitude regions of the Atlantic Rainforest of Brazil. All species presented MDL, described with two distinct morphological patterns: homogeneous or heterogeneous MDL, the latter distinguishable by MDL with dorsal or lateral expansion, sometimes up to the limit of the epidermis and comprising up to 50% of the thicknesses of the entire skin. Climate differed between locations by MDL morphological group, less rainy or seasonally less rainy where species with heterogeneous MDL occur. Our results indicate that the abundance of calcium in MDL and its heterogeneous condition suggest its adaptive function in reduce water loss. Such adaptations in anurans in very humid highlands reinforce the mountains' propensity for rapid loss of humidity, demystifying them as an extremely abundant source of water. This is the third study that tested the relationship between the MDL morphology and the environment where species occur and the first that correlated this structure with the climate of anurans of the same habit and distributed in a single habitat, the Atlantic Rainforest.
Check List, Nov 9, 2018
When Brachycephalus albolineatus was described, it was known only from its type locality in the s... more When Brachycephalus albolineatus was described, it was known only from its type locality in the state of Santa Catarina, Brazil. Here we provide a new record for the species. Collected specimens were identified in comparison with the type series. We collected 7 specimens in Morro do Garrafão, municipality of Corupá, state of Santa Catarina, at 500-530 m above sea level, in December 2016. The new record is 22 km away from the type locality and also extends the altitudinal distribution of the species, which was previously between 790-835 m above sea level.
PeerJ, Oct 3, 2018
A new miniaturized frog of the genus Brachycephalus (Anura: Brachycephalidae) is described from M... more A new miniaturized frog of the genus Brachycephalus (Anura: Brachycephalidae) is described from Morro Santo Anjo in the municipality of Massaranduba, Santa Catarina, southern Brazil. Specimens were collected from the leaf litter between 470 and 540 above sea level. The new species is distinguished from all its congeners by the combination of the following characters: (1) body robust and bufoniform; (2) size snout-vent length 9.9-11.7 mm for males and 10.0-12.9 mm for females; (3) smooth dorsum; (4) general color (in life) orange with white dots and stripe in the middle of the head and along its vertebral column; (5) iris completely black; (6) advertisement call composed of note groups; (7) isolated notes with 1-3 pulses; and (8) short isolated notes (0.002-0.027 s). An estimate of the male density of the new species is also presented. Phylogenetic information indicates that the new species is part of the southernmost clade of Brachycephalus, which includes Brachycephalus fuscolineatus, B. albolineatus, and B. boticario. The severe anthropogenic impacts in and around the type locality indicate that immediate actions should be taken to ensure the long-term preservation of the new species.
PeerJ, Oct 27, 2016
A new species of Brachycephalus (Anura: Brachycephalidae) is described from the Atlantic Forest o... more A new species of Brachycephalus (Anura: Brachycephalidae) is described from the Atlantic Forest of northeastern state of Santa Catarina, southern Brazil. Nine specimens (eight adults and a juvenile) were collected from the leaf litter of montane forests 790-835 m above sea level (a.s.l.). The new species is a member of the pernix group by its bufoniform shape and the absence of dermal co-ossification and is distinguished from all its congeners by a combination of its general coloration (dorsal region of head, dorsum, legs, arms, and flanks light, brownish green to dark, olive green, with darker region in the middle of the dorsum and a white line along the vertebral column in most specimens) and by its smooth dorsum. The geographical distribution of the new species is highly reduced (extent of occurrence estimated as 25.04 ha, or possibly 34.37 ha). In addition, its habitat has experienced some level of degradation, raising concerns about the future conservation of the species. Preliminary density estimates suggest one calling individual every 3-4 m 2 at 815-835 m a.s.l. and every 100 m 2 at 790 m a.s.l. Together with the recently described B. boticario and B. fuscolineatus, the new species is among the southernmost species of Brachycephalus known to date.
Background: Brachycephalus are among the smallest terrestrial vertebrates in the world. The genus... more Background: Brachycephalus are among the smallest terrestrial vertebrates in the world. The genus encompasses 34 species endemic to the Brazilian Atlantic Rainforest, occurring mostly in montane forests, with many species showing microendemic distributions to single mountaintops. It includes diurnal species living in the leaf litter and calling during the day, mainly during the warmer months of the year. The natural history of the vast majority of the species is unknown, such as their advertisement call, which has been described only for seven species of the genus. In the present study, we describe the advertisement call of Brachycephalus albolineatus, a recently described microendemic species from Santa Catarina, southern Brazil. Methods: We analyzed 34 advertisement calls from 20 individuals of B. albolineatus, recorded between 5 and 6 February 2016 in the type locality of the species, Morro Boa Vista, on the border between the municipalities of Jaraguá do Sul and Massaranduba, Santa Catarina, southern Brazil. We collected five individuals as vouchers (they are from the type series of the species). We used the note-centered approach to describe the advertisement calls of the species. Results: B. albolineatus have a long advertisement call of 40-191 s (mean of 88 s) composed of 8-29 notes (mean of 17 notes) emitted at a rate of 6-18 notes per minute (mean of 11 notes per minute) and at a note dominant frequency of five to seven kHz (mean of six kHz). Advertisement calls are composed of isolated notes and note groups (two notes involved in each particular note group); the former is composed by one to three pulses (mean of 2.0) and the note groups by two or three pulses in each note (mean of 2.7). Most advertisement calls present both isolated notes and note groups, with a few cases showing only the former. Note groups are emitted invariably in the last third of the advertisement call. Most isolated notes escalate their number of pulses along the advertisement call (1-2, 1-3 or 2-3). Note duration of isolated notes varies from 0.002 to 0.037 s (mean of 0.020 s) and duration of note group vary from 0.360 to 0.578 s (mean of 0.465 s). Discussion: Individuals increase the complexity of their calls as they proceed, incorporating note groups and pulses per note. Intra-individual variation analysis also demonstrated that less structured advertisement calls (i.e., with notes with fewer pulses) are not stereotyped. It is possible that isolated notes and note groups could How to cite this article Bornschein et al. (2018), Advertisement call of Brachycephalus albolineatus (Anura: Brachycephalidae).
Biological Journal of The Linnean Society, Jul 16, 2018
Despite encompassing a relatively small geographical area, montane regions harbor disproportionat... more Despite encompassing a relatively small geographical area, montane regions harbor disproportionately high levels of species diversity and endemism. Yet, relatively little is known about the evolutionary mechanisms ultimately leading to montane diversity. In this study, we use target capture of ultraconserved elements to investigate the phylogenetic relationships and diversification patterns of Melanophryniscus (Bufonidae) and Brachycephalus (Brachycephalidae), two frog genera that occur in sky islands of the southern Atlantic Forest of Brazil. Specifically, we test whether diversification of montane species in these genera can be explained by a single climatic shift leading to isolation in sky islands, followed by relative climatic stability that maintained populations in allopatry. In both genera, the topologies inferred using concatenation and coalescent-based methods were concordant and had strong nodal support, except for a few recent splits. These recent splits tended to be supported by more informative loci (those with higher average bootstrap support), suggesting that, while individual trees may be well resolved, the relationships they recover are being obscured by non-informative data. Divergence dating of a combined dataset using both genera is consistent with concordant timing of their diversification. These results support the scenario of diversification-by-isolation in sky islands, and suggest that allopatry due to climatic gradients in montane regions are an important mechanism for generating species diversity and endemism in these regions.
PeerJ, 2021
Background We are experiencing a global crisis in conservation, which has led to the prioritizati... more Background We are experiencing a global crisis in conservation, which has led to the prioritization of targets, such as nations, regions, and animal groups, which are necessary while resources are disputed. Brazil is a priority not only because of its megadiversity, high rates of endemism, and frequent descriptions of new species but also because of its high levels of deforestation. Among the species groups prioritized for conservation is the anurans (Amphibia: Anura), the population of which is severely declining. One group of anurans is the genus Brachycephalus, which includes 37 endemic species in the Brazilian Atlantic Rainforest. Some of these species have highly restricted distributions (<100 ha). Thirty new species have been described since 2000, and 55.3% of all species are threatened with extinction. Brachycephalus tridactylus was only recently described and remains restricted to its type locality. Because of its reduced geographical distribution (0.41 km2), it has been ...
Check List, 2019
Brachycephalus fuscolineatus Pie, Bornschein, Firkowski, Belmonte-Lopes & Ribeiro, 2015 was descr... more Brachycephalus fuscolineatus Pie, Bornschein, Firkowski, Belmonte-Lopes & Ribeiro, 2015 was described from Morro do Baú, Santa Catarina, Brazil. This species occurs at elevations of 640–790 m, and it is known to have an extent of occurrence of 23.63 ha. Here, we report a new record of B. fuscolineatus from Morro Braço da Onça, Luiz Alves, Santa Catarina. We recorded it on 21–22 December 2018 at elevations between 525 and 530 m; the new record occupied an area of 0.19 ha. We estimate 9 calling males in 36.35 m2.
Phyllomedusa: Journal of Herpetology, Jun 18, 2019
Redescription of the advertisement call of Brachycephalus tridactylus (Anura: Brachycephalidae). ... more Redescription of the advertisement call of Brachycephalus tridactylus (Anura: Brachycephalidae). Miniaturized frogs of the genus Brachycephalus have restricted geographical distributions throughout the Atlantic Forest. Ecological data for most species are scarce and advertisement calls have been described for only 12 of the 36 known species, including B. tridactylus. The considerable duration of note length of the advertisement call of B. tridactylus has been mentioned in comparative studies of vocalizations in Brachycephalus. The single original recording of B. tridactylus, together with recordings made in March 2016 at the type locality, were analyzed to document any variation and redescribe the advertisement call of the species. In the recent recordings of B. tridactylus, the average length of the call is 50.8 s and the call is composed of 10-13 notes emitted at a note rate of 3.7-8.3 notes per min. The notes are isolated and composed of 1-3 pulses, with the note duration varying from 0.002-0.021 s. These values are not consistent with the original description of the call of B. tridactylus. The note duration is 89% shorter and the original description does not include notes with 1-3 pulses. The notes of B. tridactylus have few pulses, whereas those of B. ephippium have several. The advertisement call of B. tridactylus differs from that of B. albolineatus and B. mirissimus by having only isolated notes.
Check List
Brachycephalus coloratus was known only from its type locality in the Serra da Baitaca of Paraná,... more Brachycephalus coloratus was known only from its type locality in the Serra da Baitaca of Paraná, Brazil. Its extent of occurrence was estimated at 0.37 km2, and its conservation status was proposed as Vulnerable. Here, we provide a second record for B. coloratus at Pão de Ló, Paraná, at 1,230 m of altitude. We estimate its current area of occupancy at 1.17 km2 and propose its conservation status as Endangered. The assessment of the Green Status suggests that it is Depleted; the prevention of deforestation and fires could lead to high conservation returns.
Este artigo teve o objetivo de analisar os riscos de doencas osteomusculares nos membros superior... more Este artigo teve o objetivo de analisar os riscos de doencas osteomusculares nos membros superiores em trabalhadores de uma industria de fabricacao de equipamentos eletricos, devido a alteracao de um sistema de trabalho, que necessita de varios trabalhadores para a montagem de um produto (modelo baseado no Taylorismo), para producao em celula, onde a montagem do produto e realizada por apenas um trabalhador (modelo baseado no Toyotismo). Para atender este objetivo foi realizado um estudo de caso onde foi utilizada a metodologia OCRA ( Occupational Repetitive Action ) que e recomendada pela norma NBR ISO 11228-3, para identificar qual dos processos (Taylorista ou Toyotista), tem a maior probabilidade de ocorrencia de doenca osteomuscular nos membros superiores dos trabalhadores envolvidos. Os resultados obtidos indicam que o sistema de producao em celulas (Toyotista), apresenta melhores condicoes ergonomicas para os membros superiores dos trabalhadores.
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Papers by Luiz Fernando Ribeiro