Papers by vandita billore
Plant Tissue Culture and Biotechnology, 2016
Plants are wonderful resource of bioproducts encompassing significant value to medicines and drug... more Plants are wonderful resource of bioproducts encompassing significant value to medicines and drug development. The plant cell suspension cultures bear immense potential for production of high-value secondary metabolites and are chosen as alternative sources of raw material for industrial use. In the present study, homogenous cell suspension culture of Celastrus paniculatus a medicinally important plant was established and multifold production of alkaloids and total phenols was obtained under the influence of monochromatic lights. One month old leaf derived friable callus of C. paniculatus was used to raise homogenous suspension culture and kept on rotary shakers in cabinets illuminated with different monochromatic LED lights (Blue, Yellow and Red). The monochromatic lights proved to be a strong abiotic elicitor in driving the production of secondary metabolites so much so that the metabolites were released extracellularly and the medium served as sink or spacious pool for leaked out metabolites from the cell mass. Maximum production and enhancement in alkaloids and phenols (98 and 44.7%, respectively) over control was obtained from cell mass grown under yellow light treatment, followed by blue (64 and 23.7%) and red light (50 and 26%) treatments. Further scale up of secondary metabolite production was hence performed under yellow light conditions, starting from 2.5 gm cell mass suspended in 250 ml of media extended up to 1000 ml culture media for one month. The continuous culture system exhibited remarkable potential of this plant cell system as multifold yield of total alkaloids
Canadian Journal of Biotechnology, Jul 28, 2017
Monochromatic lights emitted by light-emitting diodes (LEDs) have generated great interest for ef... more Monochromatic lights emitted by light-emitting diodes (LEDs) have generated great interest for efficient and controlled growth in vitro, especially of plants which are endangered or require specific intensity and wavelength of light. In the present study, we have evaluated the effect of monochromatic LEDs on in vitro morphogenesis: growth, proliferation of shoot cultures, and rooting of Dendrobium sonia. Different light sources viz. white LEDs (W), blue LEDs (B), yellow LEDs (Y) and red LEDs (R) were tested under photoperiod of 16 h of exposure and 8 h of dark. The frequency of morphogenesis depended on the wavelength of the applied monochromatic light. Higher wavelength monochromatic light (yellow light) was observed to induce higher shoot proliferation (98%), early PLB (protocorm-like bodies) formation, differentiation into green buds and shoot initiation as compared to red, blue and white light treatments. Yellow light also yielded higher number of shoots per explants (29 shoots/explant) than red, blue and white light treatments. The results suggest that the monochromatic light sources stimulate morphogenic effects on in vitro culture of Dendrobium sonia, and that yellow light treatment can be used to enhance the efficiency of micropropagation.
Research Journal of Biotechnology, 2021
Emerging infectious diseases such H1N1, MERS-CoV and severe acute respiratory diseases are now ma... more Emerging infectious diseases such H1N1, MERS-CoV and severe acute respiratory diseases are now major threats to public. Currently world is facing new threat and public emergency worldwide due to novel corona virus or Covid 2019. All of these viral diseases are a consequence of a zoonotic event and have affected millions of people around the world, some of which have resulted in high rates of morbidity/mortality in humans. The most recent zoonotic transmission SARSCoV-2 caused a great impact on the health care systems and economy around the world and affected common habits and life styles of humans. Here in this review study we will provide data and future aspects of corona virus with emergence risk factor assessment, stopping spread, therapeutic option, ongoing clinical trial and prepare future plans for control and preventing the virus spread by learning the biology by other severe acute respiratory syndrome virus (SARS).
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Papers by vandita billore