Вісник Одеського національного університету, Jun 19, 2015
Изучали состояние антиоксидантной системы у самцов и самок интродуцента Черного моря брюхоногого ... more Изучали состояние антиоксидантной системы у самцов и самок интродуцента Черного моря брюхоногого моллюска Rapana venosa, обитающего в двух разных акваториях Одесского залива. Одна из акваторий отличается более выраженной изменчивостью качества воды (соленостью, содержанием химических загрязнителей и т. п.) из-за наличия в ее зоне ливневого коллектора. Другая-характеризуется отсутствием поблизости промышленных и коммунально-хозяйственных сооружений и отличается более стабильной экологической ситуацией. В пищеводной (лейблейновской) железе рапан определяли активность супероксиддисмутазы (СОД), каталазы, глутатионпероксидазы (ГП), глутатионредуктазы (ГР) и содержание восстановленного глутатиона (GSH). Степень окислительного повреждения биополимеров оценивали по уровню содержания малонового диальдегида (МДА). У исследуемых рапан не нашли половых различий по содержанию МДА-интегрального показателя перекисного окисления липидов (ПОЛ), содержанию GSH и активности антиоксидантных ферментов, за исключением каталазы и СОД. Содержание МДА и активность СОД в пищеводной железе рапан, собранных в районе коллектора, были более высокими, а содержание GSH и активность ГП-более низкими, чем у рапан, обитающих в относительно чистом районе. Сделан вывод о высоком адаптационном потенциале R. venosa и о важности изучения роли генетических и экологических факторов в дифференцированном проявлении некоторых реакций адаптации у рапан разных акваторий Черного моря.
Transylvanian Review of Systematical and Ecological Research, 2021
Genetic structure and genetic distance of Rapana venosa populations from remote regions of the Bl... more Genetic structure and genetic distance of Rapana venosa populations from remote regions of the Black Sea (Odessa Bay and eastern Crimea, Ukraine) were defined. Despite the significant difference in morphometric parameters, the investigated samples of mollusks are genetically very similar, and the distance between them is at the level of local populations. Common features of observed groups of Rapana in the northern part of the Black Sea are the unequal occurrence of some alleles and genotypes, a high level of heterozygosity and nonequilibrium in the Hardy-Weinberg equation. The gene flow is the most significant of the evolutionary factors that form the genetic structure of Rapana venosa populations.
Transylvanian Review of Systematical and Ecological Research, 2018
The antioxidant system in various organs of rapana (Rapana venosa) has been studied in condition ... more The antioxidant system in various organs of rapana (Rapana venosa) has been studied in condition of contamination of medium with copper ions (5 and 10 MPC) and sodium dodecyl sulphate (5 and 20 MPC). The most significant changes in the antioxidant status of cells occurred mainly during the first hours (three-24 hours) of exposure to toxic compounds. At the end of observations (72 hours) stabilization of the main parameters of the antioxidant system and return of its main indicators to control values were noted. Ktenidiy proved to be the most sensitive organ reacting to toxic doses of both copper and sodium dodecyl sulphate in the medium. Nephridiy cells turned out to be weakly susceptible to the action of the studied toxicants. The high lability and power of the antioxidant system of rapans provides a significant adaptive potential for the mollusk. RÉSUMÉ: Le système antioxydant des rapana veinés sous l'action des ions de cuivre et des détergents anioniques. Le système antioxydant des différents organes des rapana veinés (Rapana venosa) a été étudié dans des conditions de contamination du milieu avec des ions de cuivre (5 et 10 MPC) et avec du lauryl sulphate de sodium (5 et 20 MPC). Les changements les plus importants dans le statut antioxydant des cellules a eu lieu durant les premières heures (trois-24 heures) d'exposition aux contaminants. A la fin des observations (72 heures) a été notée la stabilisation des principaux paramètres du système antioxydant et le retour des principaux indicateurs aux valeurs de contrôle. Les ctenidies ont été les organes les plus sensibles réagissant autant au cuivre qu'au lauryl sulphate de sodium dans le milieu. Les cellules des néphridies ont été peu sensible face à l'action des toxiques étudiés. L'étude a conclu sur lʼefficacité du système antioxydant des rapana veinés, prouvant un potentiel adaptatif significatif de ce mollusque. REZUMAT: Sistemul antioxidativ la Rapana sub acțiunea ionilor de cupru și a detergenților anionici. Sistemul antioxidativ al diferitelor organe ale rapanei (Rapana venosa) a fost studiat în condiții de contaminare a mediului cu ioni de cupru (5 și 10 MPC) și sodiu dodecil sulfat (5 și 20 MPC). Cele mai semnificative modificări în starea antioxidativă a celulelor au avut loc în special, în timpul primelor ore (trei-24 ore) de expunere la compușii toxici. La finalul perioadei de observație (72 ore) s-au notat stabilizarea principalilor parametri ai sistemului antioxidant și revenirea principalilor indicatori la valorile de control. Cele mai sensibile organe s-au dovedit a fi ctenidiile, care au reacționat atât la cupru cât și la sodiu dodecil sulfat la concentrații toxice în mediu. Celulele nefridiilor s-au dovedit a fi destul de slab susceptibile la acțiunea toxicelor studiate. Sistemul antioxidant al rapanei este foarte flexibil, ceea ce dovedește un potențial adaptativ semnificativ al acestei moluște.
Gammogobius steinitzi Bath, 1971, a goby species endemic to the Mediterranean Sea, was observed f... more Gammogobius steinitzi Bath, 1971, a goby species endemic to the Mediterranean Sea, was observed for the first time in the Black Sea in marine caves of western Crimea (Tarkhankut, Ukraine). A description of the morphological and ecological characteristics is given on the basis of the analyses of 5 specimens. The morphological and ecological characteristics of the Black Sea specimens do not differ from the Mediterranean ones.
Marine caves possess unique biocoenotic and ecological characteristics. Sessile benthic species s... more Marine caves possess unique biocoenotic and ecological characteristics. Sessile benthic species such as sponges associated with cave habitats typically show a marked zonation from the cave entrance towards the end of the cave. We describe three semi-submerged karstic caves of 50 to 83 m length and 936 to 2,291 m volume from the poorly explored cavernicolous fauna of North-East Bulgaria. We surveyed sponge diversity and spatial variability. Eight demosponge species were identified based on morphological and molecular data, of which six are known from the adjacent open sea waters of the Black Sea. Two species, van Soest & de Kluijver, 2003 and Burton, 1930, are reported from the Black Sea for the first time. The spatial sponge distribution inside the caves is in general similar, but shows some differences in species composition and distribution depending on cave relief and hydrodynamics. The species composition of sponges of Bulgarian caves is found to be different from Crimean caves....
Citation: Sidorov DA, Kovtun OA (2015) Synurella odessana sp. n. (Crustacea, Amphipoda, Crangonyc... more Citation: Sidorov DA, Kovtun OA (2015) Synurella odessana sp. n. (Crustacea, Amphipoda, Crangonyctidae), first report of a subterranean amphipod from the catacombs of Odessa and its zoogeographic importance. Subterranean Biology 15: 11-27.
Journal of the Marine Biological Association of the United Kingdom, 2015
The main objectives of this study are the establishment of a detailed description of five semi-su... more The main objectives of this study are the establishment of a detailed description of five semi-submerged and shallow-water marine caves from the Tarkhankut Peninsula (Crimea), their biological characteristics with particular attention to species composition, and the distribution of sponge assemblages in these caves. Three semi-submerged and two submerged caves with lengths of 9–131 m and volumes of 61–3060 m3have been investigated. All of them are karst-abrasive or karst in origin. In the investigated caves, we inventoried seven sponge species. All were recorded species of Porifera belong to the class Demospongiae and have previously been recorded also in adjacent open sea waters. These species are tolerant to different hydrological conditions, mostly temperature and salinity. Some of them have wide geographic distribution. The species composition of sponges from the shallow water caves of Crimea is quite different from the sponge composition in Mediterranean caves. This could be du...
The medusa, Aurelia aurita (Scyphozoa, Cnidaria), is counted as a cosmopolitan species with a wor... more The medusa, Aurelia aurita (Scyphozoa, Cnidaria), is counted as a cosmopolitan species with a worldwide distribution in most seas, from the poles to the tropics. Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by huge mesoglea in medusa. The main medusas' morphology is similar in different populations. Previously the new protein ''mesoglein'' was determined as one of the main components of mesoglea. Deduced amino acid sequence of mesoglein contains Zona Pellucida (ZP) domain. The comparison of mesoglein and its gene from three habitats White Sea (WsA), Black Sea (BsA), Japonic Sea (JsA) has been done in the current work. The set of the mesoglea protein bands after SDS-PAGE is similar in all samples. Nevertheless, JsA mesogleins' M r is 53\55 kDa, while WsA and BsA mesogleins have M r of 47 kDa. Antibodies raised against WsA mesoglein recognize only mesoglein with M r of 47 kDa, but not 53\55 kDa, both on immunoblot and immunocytochemistry. Mesogleal cells and ''elastic'' fibrils are stained intensively in the mesoglea both from WsA and BsA but not from JsA. The possibility of gene diversity was checked by PCR with primers specific for WsA mesoglein gene. PCR products of expected length were obtained on cDNA from polyA-RNA template from mesogleal cells of WsA and BsA medusa but not on cDNA of JsA medusa. Our results evidence that there are two different species in genus Aurelia: Aurelia aurita inhabits White and Black Seas while Aurelia sp.1 inhabits Japonic Sea. Such a suggestion is in agreement with the one previously done on the base of comparison by other molecular biology methods.
Вісник Одеського національного університету, Jun 19, 2015
Изучали состояние антиоксидантной системы у самцов и самок интродуцента Черного моря брюхоногого ... more Изучали состояние антиоксидантной системы у самцов и самок интродуцента Черного моря брюхоногого моллюска Rapana venosa, обитающего в двух разных акваториях Одесского залива. Одна из акваторий отличается более выраженной изменчивостью качества воды (соленостью, содержанием химических загрязнителей и т. п.) из-за наличия в ее зоне ливневого коллектора. Другая-характеризуется отсутствием поблизости промышленных и коммунально-хозяйственных сооружений и отличается более стабильной экологической ситуацией. В пищеводной (лейблейновской) железе рапан определяли активность супероксиддисмутазы (СОД), каталазы, глутатионпероксидазы (ГП), глутатионредуктазы (ГР) и содержание восстановленного глутатиона (GSH). Степень окислительного повреждения биополимеров оценивали по уровню содержания малонового диальдегида (МДА). У исследуемых рапан не нашли половых различий по содержанию МДА-интегрального показателя перекисного окисления липидов (ПОЛ), содержанию GSH и активности антиоксидантных ферментов, за исключением каталазы и СОД. Содержание МДА и активность СОД в пищеводной железе рапан, собранных в районе коллектора, были более высокими, а содержание GSH и активность ГП-более низкими, чем у рапан, обитающих в относительно чистом районе. Сделан вывод о высоком адаптационном потенциале R. venosa и о важности изучения роли генетических и экологических факторов в дифференцированном проявлении некоторых реакций адаптации у рапан разных акваторий Черного моря.
Transylvanian Review of Systematical and Ecological Research, 2021
Genetic structure and genetic distance of Rapana venosa populations from remote regions of the Bl... more Genetic structure and genetic distance of Rapana venosa populations from remote regions of the Black Sea (Odessa Bay and eastern Crimea, Ukraine) were defined. Despite the significant difference in morphometric parameters, the investigated samples of mollusks are genetically very similar, and the distance between them is at the level of local populations. Common features of observed groups of Rapana in the northern part of the Black Sea are the unequal occurrence of some alleles and genotypes, a high level of heterozygosity and nonequilibrium in the Hardy-Weinberg equation. The gene flow is the most significant of the evolutionary factors that form the genetic structure of Rapana venosa populations.
Transylvanian Review of Systematical and Ecological Research, 2018
The antioxidant system in various organs of rapana (Rapana venosa) has been studied in condition ... more The antioxidant system in various organs of rapana (Rapana venosa) has been studied in condition of contamination of medium with copper ions (5 and 10 MPC) and sodium dodecyl sulphate (5 and 20 MPC). The most significant changes in the antioxidant status of cells occurred mainly during the first hours (three-24 hours) of exposure to toxic compounds. At the end of observations (72 hours) stabilization of the main parameters of the antioxidant system and return of its main indicators to control values were noted. Ktenidiy proved to be the most sensitive organ reacting to toxic doses of both copper and sodium dodecyl sulphate in the medium. Nephridiy cells turned out to be weakly susceptible to the action of the studied toxicants. The high lability and power of the antioxidant system of rapans provides a significant adaptive potential for the mollusk. RÉSUMÉ: Le système antioxydant des rapana veinés sous l'action des ions de cuivre et des détergents anioniques. Le système antioxydant des différents organes des rapana veinés (Rapana venosa) a été étudié dans des conditions de contamination du milieu avec des ions de cuivre (5 et 10 MPC) et avec du lauryl sulphate de sodium (5 et 20 MPC). Les changements les plus importants dans le statut antioxydant des cellules a eu lieu durant les premières heures (trois-24 heures) d'exposition aux contaminants. A la fin des observations (72 heures) a été notée la stabilisation des principaux paramètres du système antioxydant et le retour des principaux indicateurs aux valeurs de contrôle. Les ctenidies ont été les organes les plus sensibles réagissant autant au cuivre qu'au lauryl sulphate de sodium dans le milieu. Les cellules des néphridies ont été peu sensible face à l'action des toxiques étudiés. L'étude a conclu sur lʼefficacité du système antioxydant des rapana veinés, prouvant un potentiel adaptatif significatif de ce mollusque. REZUMAT: Sistemul antioxidativ la Rapana sub acțiunea ionilor de cupru și a detergenților anionici. Sistemul antioxidativ al diferitelor organe ale rapanei (Rapana venosa) a fost studiat în condiții de contaminare a mediului cu ioni de cupru (5 și 10 MPC) și sodiu dodecil sulfat (5 și 20 MPC). Cele mai semnificative modificări în starea antioxidativă a celulelor au avut loc în special, în timpul primelor ore (trei-24 ore) de expunere la compușii toxici. La finalul perioadei de observație (72 ore) s-au notat stabilizarea principalilor parametri ai sistemului antioxidant și revenirea principalilor indicatori la valorile de control. Cele mai sensibile organe s-au dovedit a fi ctenidiile, care au reacționat atât la cupru cât și la sodiu dodecil sulfat la concentrații toxice în mediu. Celulele nefridiilor s-au dovedit a fi destul de slab susceptibile la acțiunea toxicelor studiate. Sistemul antioxidant al rapanei este foarte flexibil, ceea ce dovedește un potențial adaptativ semnificativ al acestei moluște.
Gammogobius steinitzi Bath, 1971, a goby species endemic to the Mediterranean Sea, was observed f... more Gammogobius steinitzi Bath, 1971, a goby species endemic to the Mediterranean Sea, was observed for the first time in the Black Sea in marine caves of western Crimea (Tarkhankut, Ukraine). A description of the morphological and ecological characteristics is given on the basis of the analyses of 5 specimens. The morphological and ecological characteristics of the Black Sea specimens do not differ from the Mediterranean ones.
Marine caves possess unique biocoenotic and ecological characteristics. Sessile benthic species s... more Marine caves possess unique biocoenotic and ecological characteristics. Sessile benthic species such as sponges associated with cave habitats typically show a marked zonation from the cave entrance towards the end of the cave. We describe three semi-submerged karstic caves of 50 to 83 m length and 936 to 2,291 m volume from the poorly explored cavernicolous fauna of North-East Bulgaria. We surveyed sponge diversity and spatial variability. Eight demosponge species were identified based on morphological and molecular data, of which six are known from the adjacent open sea waters of the Black Sea. Two species, van Soest & de Kluijver, 2003 and Burton, 1930, are reported from the Black Sea for the first time. The spatial sponge distribution inside the caves is in general similar, but shows some differences in species composition and distribution depending on cave relief and hydrodynamics. The species composition of sponges of Bulgarian caves is found to be different from Crimean caves....
Citation: Sidorov DA, Kovtun OA (2015) Synurella odessana sp. n. (Crustacea, Amphipoda, Crangonyc... more Citation: Sidorov DA, Kovtun OA (2015) Synurella odessana sp. n. (Crustacea, Amphipoda, Crangonyctidae), first report of a subterranean amphipod from the catacombs of Odessa and its zoogeographic importance. Subterranean Biology 15: 11-27.
Journal of the Marine Biological Association of the United Kingdom, 2015
The main objectives of this study are the establishment of a detailed description of five semi-su... more The main objectives of this study are the establishment of a detailed description of five semi-submerged and shallow-water marine caves from the Tarkhankut Peninsula (Crimea), their biological characteristics with particular attention to species composition, and the distribution of sponge assemblages in these caves. Three semi-submerged and two submerged caves with lengths of 9–131 m and volumes of 61–3060 m3have been investigated. All of them are karst-abrasive or karst in origin. In the investigated caves, we inventoried seven sponge species. All were recorded species of Porifera belong to the class Demospongiae and have previously been recorded also in adjacent open sea waters. These species are tolerant to different hydrological conditions, mostly temperature and salinity. Some of them have wide geographic distribution. The species composition of sponges from the shallow water caves of Crimea is quite different from the sponge composition in Mediterranean caves. This could be du...
The medusa, Aurelia aurita (Scyphozoa, Cnidaria), is counted as a cosmopolitan species with a wor... more The medusa, Aurelia aurita (Scyphozoa, Cnidaria), is counted as a cosmopolitan species with a worldwide distribution in most seas, from the poles to the tropics. Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by huge mesoglea in medusa. The main medusas' morphology is similar in different populations. Previously the new protein ''mesoglein'' was determined as one of the main components of mesoglea. Deduced amino acid sequence of mesoglein contains Zona Pellucida (ZP) domain. The comparison of mesoglein and its gene from three habitats White Sea (WsA), Black Sea (BsA), Japonic Sea (JsA) has been done in the current work. The set of the mesoglea protein bands after SDS-PAGE is similar in all samples. Nevertheless, JsA mesogleins' M r is 53\55 kDa, while WsA and BsA mesogleins have M r of 47 kDa. Antibodies raised against WsA mesoglein recognize only mesoglein with M r of 47 kDa, but not 53\55 kDa, both on immunoblot and immunocytochemistry. Mesogleal cells and ''elastic'' fibrils are stained intensively in the mesoglea both from WsA and BsA but not from JsA. The possibility of gene diversity was checked by PCR with primers specific for WsA mesoglein gene. PCR products of expected length were obtained on cDNA from polyA-RNA template from mesogleal cells of WsA and BsA medusa but not on cDNA of JsA medusa. Our results evidence that there are two different species in genus Aurelia: Aurelia aurita inhabits White and Black Seas while Aurelia sp.1 inhabits Japonic Sea. Such a suggestion is in agreement with the one previously done on the base of comparison by other molecular biology methods.
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