Papers by Md. Shalahuddin Millat
Human Gene
Background: Transcription factor 21 (TCF21) genetic polymorphisms are correlated with different c... more Background: Transcription factor 21 (TCF21) genetic polymorphisms are correlated with different cardiovascular diseases (CVDs). TCF21 rs12190287 polymorphism at the 3 ′-untranslated region has been studied in Asian and African ethnicities for the risk of CVDs that resulted in inconsistent outcomes. Therefore, the present metaanalysis aimed to evaluate the association between TCF21 rs12190287 polymorphism and CVDs risk for the first time. Methods: An electronic database search was performed for relevant articles following the latest Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. This meta-analysis included six studies consisting of 7725 cases and 6770 controls up to October 2021 to analyze the association by pooled odds ratio (OR) with 95% confidence intervals (95% CI). The association was calculated for seven genetic association models using RevMan 5.4 software. Results: The results revealed a statistically significant association of TCF21 rs12190287 with overall CVDs risk in five genetic models (co-dominant model 2: OR = 1.36, 95% CI = 1.
Crude methanolic extract of Crinum latifolium plant (Leaves) was assayed to identify various phar... more Crude methanolic extract of Crinum latifolium plant (Leaves) was assayed to identify various pharmacological properties. Antimicrobial potential of crude methanolic extracts of Crinum latifolium was accomplished by most commonly used disc diffusion method against a wide range of Gram positive and Gram negative bacteria. Extracts showed slight antimicrobial activity against Gram positive bacteria while surprisingly showed significant antimicrobial activity against Gram negative bacteria E. coli. In contrast to Vincristine Sulphate, the crude metanolic, n-hexane soluble, petroleum ether soluble and chloroform soluble extracts showed slight to moderate cytotoxic properties with LC 50 value of 7.06µg/ml, 48.978µg/ml, 242.83µg/ml and 153.93µg/ml respectively. Plant extract showed significant anti-inflammatory properties i.e 16.21% & 20.55%10mg/ml for hypotonic solution and heat induced condition respectively. So, this plant extract demands further research for revealing all its potency t...
Atorvastatin calcium is a poorly water soluble compound marketed in Bangladesh under bio-waiver c... more Atorvastatin calcium is a poorly water soluble compound marketed in Bangladesh under bio-waiver conditions. The present study aimed to develop formulation and drug excipients compatibility study of Atorvastatin calcium (50 mg) sustain release tablet and optimize the final formula. The tablet were formulated by direct compression method and the results obtained were extrapolated. Solid dispersion of Atorvastatin calcium was prepared by using Hydroxypropyl methyl cellulose, Methyl cellulose, Lactose, Guar gum, Xanthan gum & Magnesium Stearate. The in vitro equivalence test was carried out in three different media. Test results were subjected to statistical analysis to compare the dissolution profiles. Other general quality parameters of these tablets such as weight variation, friability, thickness, hardness and disintegration time were also determined according to established protocols. Final formulation of solid dispersed Atorvastatin calcium revealed that successfully improvement of...
Discovery Phytomedicine, 2019
The purpose of the present study was to evaluate the antidiarrheal activity of methanol extracts ... more The purpose of the present study was to evaluate the antidiarrheal activity of methanol extracts of Brassica nigra flower using castor oilinduced diarrhoea and gastrointestinal motility test using charcoal meal method. The methanolic extract was initially fractioned which was assayed for its effect in castor oil-induced diarrhoea at different doses (200 and 400 mg/kg body weight) followed by its evaluation on the peristaltic movements in charcoal meal test using loperamide as a reference drug. The results of the present study indicated that, administration of the various fractions of methanolic extract of Brassica nigra induced dose-dependent percentages of inhibition of diarrhoea. The antidiarrheal potential of this plant might be due to its high contents of flavonoids and tannins. It could be claimed that, the remarkable antidiarrheal activity of Brassica nigra flower support to its utility in a wide range of remedies of diarrhoea.
Journal of the Egyptian Public Health Association, 2021
Background The outbreak of coronavirus infectious disease-2019 (COVID-19) is globally deemed a si... more Background The outbreak of coronavirus infectious disease-2019 (COVID-19) is globally deemed a significant threat to human life. Researchers are searching for prevention strategies, mitigation interventions, and potential therapeutics that may reduce the infection’s severity. One such means that is highly being talked in online and in social media is vitamin C. Main text Vitamin C is a robust antioxidant that boosts the immune system of the human body. It helps in normal neutrophil function, scavenging of oxidative species, regeneration of vitamin E, modulation of signaling pathways, activation of pro-inflammatory transcription factors, activation of the signaling cascade, regulation of inflammatory mediators, and phagocytosis and increases neutrophil motility to the site of infection. All of these immunological functions are required for the prevention of COVID-19 infection. Conclusion Considering the role of vitamin C, it would be imperative to administrate vitamin C for the manag...
Novel Approaches in Drug Designing & Development, 2017
The present study was aimed to assess ethno medicinal value of the plant. We tried to oversee in-... more The present study was aimed to assess ethno medicinal value of the plant. We tried to oversee in-vitro antimicrobial, thrombolytic and membrane stabilizing potentials of chloroform extracts of Avicennia marina leaves and barks. A. marina is an ethno medicinal plant belongs to the family of Acanthaceae. Crude chloroform extracts of A. marina (leaves & barks) dose dependently used for in vitro antimicrobial, thrombolytic and membrane stability screening. Chloroform extracts of A. marina at higher concentration evolved moderate antimicrobial activities against Gram negative (-ve) bacteria and were resistant against Gram positive (+ve) bacteria during assessment through disc diffusion method. During analysis of thrombolytic activity, 10mg/ml concentration of the extracts most significantly showed 16.33±0.49% lysis of clot (p<0.001), where streptokinase was used as standard & distilled water was treated as negative control. Again, Acetyl salicylic acid was used as standard in membrane stabilizing study whereas chloroform extracts of A.marina leaves and barks dose dependently showed inhibition of hemolysisat 10mg/ml, which was found 18.84±0.99% and 26.24±1.04% respectively for both hypotonic solution and heat induced condition of hemolysis of erythrocyte membrane.
Objectives: The present study was intended to investigate possible phytochemical nature (group de... more Objectives: The present study was intended to investigate possible phytochemical nature (group determinant of plant extract) and to reveal in-vitro thrombolytic, membrane stabilizing and cytotoxic activities of crude methanolic extracts of Bruguiera cylindrica leaves. Methods: Collected plant parts were cleaned, dried, and ground to obtain powdery mass. Various methanolic extracts of B. cylindrica was subjected for evaluating membrane stabilizing potentials at a hypotonic solution and heat induce condition, where thrombolytic activity assessment was done by employing Streptokinase as standard. Finally, Cytotoxicity activity was determined by Brine shrimp Lethality bioassay. Results: Phytochemical screening of methanolic extracts revealed the presence of carbohydrate(s), glycoside(s), phenol, tannin, protein(s), gum and mucilages. Crude methanolic extracts with different doses were used for in-vitro thrombolytic study and 10 mg/ml concentration of plant extracts showed moderate clot ...
MicroRNA, 2021
Background: The prevalence of Cervical Cancer (CC) is disproportionately higher in developing cou... more Background: The prevalence of Cervical Cancer (CC) is disproportionately higher in developing countries. It is the second most frequent cancer type among Bangladeshi women and the major cause of morbidity and mortality. However, no previous data reported the association of miR-218-2 gene polymorphisms in Bangladeshi cervical cancer patients. Aim: This case-control study was designed to find the link between the rs11134527 polymorphism in miR-218-2 and CC. Methods: A total of 488 subjects were recruited, comprising 256 cervical cancer patients and 232 healthy females. Genotyping was conducted with the tetra-primer ARMS-PCR technique to detect the association. Results: The results of genotype data showed that rs11134527 was in the Hardy-Weinberg equilibrium in both CC cases and controls (P >0.05). Overall, the polymorphism was found to be significantly associated with an increased risk of cervical cancer with AG genotype (AG vs. GG: OR = 2.26, 95% Cl = 1.40-3.66, P = 0.0008), AA ge...
Heliyon, 2020
Background Ischemic heart disease (IHD) is a major cause of death globally. Countries vary in the... more Background Ischemic heart disease (IHD) is a major cause of death globally. Countries vary in their rates, and changes have occurred over time. Nowadays, developing countries pose new public health challenges. Objectives The objective of the present study was to appraise the alterations in the levels of serum Zn, Cu, Fe, and Mn that occur in patients with ischemic heart disease and to depict the correlations of the effects of these changes that lead to the pathogenesis of IHD. Methods Zn, Cu, Fe, and Mn in the IHD patients were determined by Atomic Absorption Spectroscopy (AAS). Results This study evaluated 52 patients with IHD, and 61 healthy volunteers served as controls. The primary outcomes of interest were explored regarding the correlations of the serum levels of these trace elements in patients with IHD. The secondary outcomes were explored in terms of inter-element relations to connect them with the pathogenesis of IHD. Our study found significantly reduced levels of Zn and ...
Inquisition with methanolic extract of Vigna unguiculata Linn (seeds) was carried out to determin... more Inquisition with methanolic extract of Vigna unguiculata Linn (seeds) was carried out to determine the thrombolytic potential of this plant. Plant seeds were extracted with methanol at room temperature. Then concentrated methanolic extract five different concentration (2mg/ml, 4mg/ml, 6mg/ml, 8mg/ml, 10mg/ml) from concentrated methanolic extract was used to evaluate thrombolytic potential of Vigna unguiculata seeds. An easy & rapid methodology (In-vitro Thrombolytic model) was applied to find out their thrombolytic potential where streptokinase and distilled water were employed as positive and negative controls respectively. The plant showed significant clot lysis, i.e. concentrations 12.01 ± 1.50, 16.48 ± 2.31,24.88 ± 1.49,31.24 ± 0.68,40.33 ± 3.64 at 2mg/dl, 4mg/ml, 6mg/ml, 8mg/ml, 10mg/ml respectively, while the standard (streptokinase) and negative control (distilled water) showed 58.41 ± 3.71 and 2.56±1.23% clot lysis respectively. It is clear that Vigna unguiculata Linn (seed)...
Heliyon, 2021
Type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) are two deadly diseases caused b... more Type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) are two deadly diseases caused by the complex interaction of multiple genetic loci, lifestyle and environmental factors. Genome-wide association studies described hundreds of susceptibility loci for T2DM and T2DM-related CVD, but it remains uncertain due to geographic and ethnic variations. The objective of this study was to evaluate the associations of KCNJ11 rs5219, SLC30A8 rs13266634 and HHEX rs1111875 polymorphisms with T2DM and related CVD. Genotyping of all three polymorphisms was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method on 250 T2DM cases and 246 healthy controls. Both descriptive and inferential statistical methods were applied using MedCalc and IBM SPSS software programs for statistical analyses. A significantly increased association of KCNJ11 rs5219 (p<0.05) with T2DM was found in dominant, recessive, heterozygote, homozygote, and allele model (aOR = 2.23, 2.03, 1.90, 3.09, and 1.80, respectively). For SLC30A8 rs13266634, only dominant, heterozygote, and allele model (aOR = 3.37, 3.59, and 1.79, respectively) showed significantly increased association with T2DM. SNP rs1111875 (HHEX) also revealed 2.08, 4.18, 5.93, and 2.08-times significant association in dominant, recessive, homozygote, and allele models. Besides, a significantly reduced correlation of KCNJ11 rs5219 was found with T2DM-related CVD in the recessive and allele model (aOR = 0.40 and 0.65, respectively). Again, a significant difference was observed between T2DM-related CVD and non-CVD patients in terms of gender distribution, fasting blood glucose (FBG), systolic blood pressure (SBP), diastolic blood pressure (DBP), total cholesterol (TC), and triglycerides (TG). Our investigation indicates that KCNJ11 rs5219, SLC30A8 rs13266634 and HHEX rs1111875 polymorphisms are associated with T2DM. Moreover, KCNJ11 rs5219 polymorphism is correlated with the risk of T2DM-related CVD.
Meta Gene, 2021
Abstract Objectives Prostate cancer is the most frequent non-cutaneous malignancy in men. Numerou... more Abstract Objectives Prostate cancer is the most frequent non-cutaneous malignancy in men. Numerous genetic factors play a crucial role in the progression of prostate cancer. This study was undertaken to reveal the correlation of TP53 rs1042522 and CDH1 rs16260 polymorphisms with the risk of prostate cancer in the Bangladeshi population. Materials and methods We recruited 210 prostate cancer patients and 210 healthy controls for the investigation. Genotyping was conducted using the PCR-RFLP technique. Results In case of TP53 gene rs1042522, the association analysis revealed that all genetic models were linked with significantly increased risk for prostate cancer development (CG vs. CC: OR = 1.99, p = 0.001; GG vs. CC: OR = 4.82, p Conclusions Our study indicates a significant association of TP53 rs1042522 and CDH1 rs16260 polymorphisms with increased prostate cancer risk in the Bangladeshi population. However, replication studies are required in different populations to validate our findings.
Human Cell, 2021
Autism spectrum disorder (ASD) is a multifactorial neurodevelopmental disorder characterized by c... more Autism spectrum disorder (ASD) is a multifactorial neurodevelopmental disorder characterized by communication deficits, impaired social interactions, repetitive and stereotyped behaviors with restricted interests, and connected with the interaction between environmental factors and genetic vulnerability. CNTNAP2 gene has been extensively investigated for ASD and related neurodevelopment diseases. However, previous studies have resulted in an inconsistent outcome. Based on this fact, we conducted a case-control study followed by a meta-analysis to investigate the association of rs7794745 and rs2710102 polymorphisms with ASD. A total of 216 autistic children and 240 healthy volunteers were recruited, and genotyping was performed using the PCR-RFLP method. We observed that SNP rs7794745 revealed a significantly (p < 0.05) increased association with the development of ASD in children in all genetic models. No significant association was found for rs2710102 with ASD. Besides, rs2710102 exhibited a significant association with language impairment in TC genotype, C allele, and dominant model. From the meta-analysis of both SNPs, we found a significant association in codominant 1, 2, and the dominant model of rs2710102 and codominant 1 and dominant model of rs7794745 with ASD. Our case-control study suggests that rs7794745 polymorphism is associated with ASD, while rs2710102 is correlated with language impairment. Moreover, meta-analysis results indicated the association between both rs7794745 and rs2710102 polymorphisms and ASD.
International Journal of Biological Research, 2016
Quisqualis indica plant (Stem bark) was analyzed in-vitro to evaluate biological activities inclu... more Quisqualis indica plant (Stem bark) was analyzed in-vitro to evaluate biological activities including anti-microbial properties and cytotoxic potentials. The crude n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates of the methanolic extract of the plant were subjected to the microbiological investigation against a wide range of Gram positive, Gram negative bacteria and fungi. Brine shrimp lethality bioassay was used for evaluation of cytotoxic activity of Quisqualis indica. The zones of inhibition produced by the hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates of the methanolic extract of Quisqualis indica were ranged from 7-15 mm, 8-18 mm, 12-20 mm and 10-16 mm respectively at a concentration of 400μg/disc. The obtained results were compared against standard Kanamycin (30µg/disc). In case of brine shrimp lethality bioassay, the n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were found to show LC50 of...
International Immunopharmacology, 2021
BACKGROUND Cervical cancer (CC) is the main cause of cancer-related deaths among women in develop... more BACKGROUND Cervical cancer (CC) is the main cause of cancer-related deaths among women in developing countries. It is the second leading female malignancy in Bangladesh in terms of incidence and mortality. Our present study aimed to investigate the association of IL1β (rs16944), IL4R (rs1801275), and IL6 (rs1800797) gene polymorphisms with the susceptibility of cervical cancer. MATERIALS AND METHODS This case-control study was conducted on 252 cervical cancer patients and 228 healthy volunteers, using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). RESULTS In the case of rs16944 polymorphism, GG genotype (OR = 2.10, 95%CI = 1.24-3.56), dominant model (OR = 1.71, 95% CI = 1.11-2.63), recessive model (OR = 1.54, 95% CI = 1.01-2.35), and G allele (OR = 1.30, 95% CI = 1.005-1.68) were significantly associated with increased cervical cancer risk. Among these, GG genotype and dominant model remained significant after the Bonferroni correction (p < 0.017). For rs1801275 polymorphism, GG genotype (OR = 2.66, 95% CI = 1.49-4.75), dominant model (OR = 1.49, 95% CI = 1.04-2.14), recessive model (OR = 2.45, 95% CI = 1.40-4.27), and G allele (OR = 1.59, 95% CI = 1.21-2.10) significantly elevated the risk of cervical cancer but significance did not exist for dominant model after the Bonferroni correction. rs1800797 variant showed significantly increased risk in all genetic models including, AG genotype (OR = 8.13, 95% CI = 5.27-12.55), AA genotype (OR = 9.86, 95% CI = 2.76-35.21), dominant model (OR = 8.25, 95% CI = 5.40-12.60), recessive model (OR = 4.41, 95% CI = 1.25-15.56), and A allele (OR = 4.99, 95% CI = 3.49-7.13) and the significances were consistent with the Bonferroni correction except recessive model. Haplotyping analysis indicates that GAA (p = 5.15x10-5) and GAG haplotypes (p = 4.72x10-9) significantly decreased the risk of CC, whereas AAA (p = 3.89x10-9), AAG (p = 0.0003), AGA (p = 3.98x10-5) and AGG haplotypes (p = 0.002) significantly increased the risk of CC. The IL1β mRNA level was up-regulated, which was associated with poor prognosis in silico. CONCLUSION Our results conclude that rs16944 (IL1β), rs1801275 (IL4R), and rs1800797 (IL6) polymorphisms are associated with cervical cancer in Bangladeshi women.
Heliyon, 2020
DRD2 gene is considered one of the most important candidate genes for the schizophrenia (SCZ) dev... more DRD2 gene is considered one of the most important candidate genes for the schizophrenia (SCZ) development due to its role in dopamine signaling and no genetic association study has been conducted yet on the Bangladeshi SCZ patients. The objective of the present study was to investigate the association of DRD2 genetic polymorphisms (rs4648317, rs4936270, and rs7131056) with SCZ in the Bangladeshi population. Patients and methods: This case-control study consisted of 101 SCZ patients and 101 controls. Genotyping was performed by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method. Results: The average ages were 22.15 and 22.09 years in patients and controls, respectively (p > 0.05). CT genotype of rs4936270 showed a significantly higher risk for the development of SCZ compared to CC genotype (OR ¼ 2.0, p ¼ 0.023), whereas no association was found for TT genotype. For the dominant model and T allele, rs4936270 showed a higher risk for the development of SCZ (OR ¼ 2.01, p ¼ 0.020; OR ¼ 1.76, p ¼ 0.021, respectively), while the recessive model had no association with SCZ. A statistically significant (OR ¼ 2.70, p ¼ 0.036) higher risk was found for the AA genotype, but no association was found for GA genotype of rs4648317 SNP compared to GG genotype. In case of dominant and recessive models, rs4648317 showed no association with SCZ. 'A' allele of rs4648317 SNP was found to be significantly associated with the elevated risk of SCZ (OR ¼ 1.50, p ¼ 0.044). No association with SCZ of rs7131056 SNP was found for AC, CC genotypes, dominant, recessive, and allele models. Furthermore, from the haplotyping analysis, we found that CAA and TAA haplotypes of rs4936270, rs7131056 and rs4648317 SNPs are associated with SCZ (χ2 ¼ 8.26, p ¼ 0.004; χ2 ¼ 5.31, p ¼ 0.021, respectively). After Bonferroni correction, the association of SCZ did not withstand with any genotype, allele and haplotype (p < 0.017) except CAA haplotype. Conclusion: Our results suggest that DRD2 gene polymorphisms may be associated with the susceptibility of SCZ in the young Bangladeshi population.
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Papers by Md. Shalahuddin Millat