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Jayne Wallace

Northumbria University, School of Design, Reader in Craft Futures

Newcastle University, Culture Lab, Alumna

Sheffield Hallam University, Art and Design, Alumna

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Steering Committee member and Documentation Chair - Research Through Design 2015 conference.
Founding member (alongside Dr Joyce Yee) of Research Through Design International biennial conference.
General Chair - Praxis and Poetics: Research Through Design 2013 Conference.

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Papers by Jayne Wallace

Highly parallel direct RNA sequencing on an array of nanopores

Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us... more Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing – information that is useful for understanding the status and function of a sample. Oxford Nanopore Technologies’ sequencing technology is capable of electronically analysing a sample’s DNA directly, and in real-time. In this manuscript we demonstrate the ability of an array of nanopores to sequence RNA directly, and we apply it to a range of biological situations. Nanopore technology is the only available sequencing technology that can sequence RNA directly, rather than depending on reverse transcription and PCR. There are several potential advantages of this approach over other RNA-seq strategies, including the absence of amplification and reverse transcription biases, the ability to detect nucleotide analogues and the ability to generate full-length, s...

Crystal structure of an invertebrate cytolysin pore reveals unique properties and mechanism of assembly

Nature communications, May 12, 2016

The invertebrate cytolysin lysenin is a member of the aerolysin family of pore-forming toxins tha... more The invertebrate cytolysin lysenin is a member of the aerolysin family of pore-forming toxins that includes many representatives from pathogenic bacteria. Here we report the crystal structure of the lysenin pore and provide insights into its assembly mechanism. The lysenin pore is assembled from nine monomers via dramatic reorganization of almost half of the monomeric subunit structure leading to a β-barrel pore ∼10 nm long and 1.6-2.5 nm wide. The lysenin pore is devoid of additional luminal compartments as commonly found in other toxin pores. Mutagenic analysis and atomic force microscopy imaging, together with these structural insights, suggest a mechanism for pore assembly for lysenin. These insights are relevant to the understanding of pore formation by other aerolysin-like pore-forming toxins, which often represent crucial virulence factors in bacteria.

Free-Energy Calculations Reveal the Subtle Differences in the Interactions of DNA Bases with α-Hemolysin

Journal of Chemical Theory and Computation, 2015

Next generation DNA sequencing methods that utilize protein nanopores have the potential to revol... more Next generation DNA sequencing methods that utilize protein nanopores have the potential to revolutionize this area of biotechnology. While the technique is underpinned by simple physics, the wild-type protein pores do not have all of the desired properties for efficient and accurate DNA sequencing. Much of the research efforts have focused on protein nanopores, such as α-hemolysin from Staphylococcus aureus. However, the speed of DNA translocation has historically been an issue, hampered in part by incomplete knowledge of the energetics of translocation. Here we have utilized atomistic molecular dynamics simulations of nucleotide fragments in order to calculate the potential of mean force (PMF) through α-hemolysin. Our results reveal specific regions within the pore that play a key role in the interaction with DNA. In particular, charged residues such as D127 and K131 provide stabilizing interactions with the anionic DNA and therefore are likely to reduce the speed of translocation. These regions provide rational targets for pore optimization. Furthermore, we show that the energetic contributions to the protein−DNA interactions are a complex combination of electrostatics and short-range interactions, often mediated by water molecules.

Barriers to patient service innovation in the UK National Health Service (NHS): A case study of seeking permission to conduct service design research for long term conditions, specifically Type 1 diabetes

Size and Number of Epithelial Cells Washed from the Cornea after Contact Lens Wear

Optometry and Vision Science, 1998

Carbon nanotube self-assembly with lipids and detergent: a molecular dynamics study

Nanotechnology, 2008

The dispersion of carbon nanotubes (CNTs) in aqueous media is of potential importance in a number... more The dispersion of carbon nanotubes (CNTs) in aqueous media is of potential importance in a number of biomedical applications. CNT solubilization has been achieved via the non-covalent adsorption of lipids and detergent onto the tube surface. We use coarse-grained molecular dynamics to study the self-assembly of CNTs with various amphiphiles, namely a bilayer-forming lipid, dipalmitoylphosphatidylcholine (DPPC), and two species of detergent, dihexanoylphosphatidylcholine (DHPC) and lysophosphatidylcholine (LPC). We find that for a low amphiphile/CNT ratio, DPPC, DHPC and LPC all wrap around the CNT. Upon increasing the number of amphiphiles, a transition in adsorption is observed: DPPC encapsulates the CNT within a cylindrical micelle, whilst both DHPC and LPC adsorb onto CNTs in hemimicelles. This study highlights differences in adsorption mechanism of bilayer-forming lipids and detergents on CNTs which may in the future be exploitable to enable enhancement of CNT solubilization whilst minimizing perturbation of cell membrane integrity.

Carbon nanotube/detergent interactions via coarse-grained molecular dynamics

Chemistry and Physics of Lipids, 2007

ReFind: Design, Lived Experience and Ongoingness in Bereavement

We describe the design and use of ReFind, a handheld artefact made for people who are bereaved an... more We describe the design and use of ReFind, a handheld artefact made for people who are bereaved and are ready to re-explore their relationship to the deceased person. ReFind was made within a project seeking to develop new ways to curate and create digital media to support ongoingness-an active, dynamic component of continuing bonds. We draw on bereavement theory and care championing practices that enable a continued sense of connection between someone bereaved and a person who has died. We present the design development of ReFind and the lived experience of the piece by the first author. We discuss our wider methodology which includes autobiographical design and reflections on if and how the piece supported ongoing connections, the challenges faced, and insights gained.

Empathetic Design for and with Vulnerable Women in Secure Hospital Services

We present our work in progress on the design process of a set of interactive objects, the Sphere... more We present our work in progress on the design process of a set of interactive objects, the Spheres of Wellbeing, for and with women in secure hospital services. The women present a very vulnerable and difficult-to-treat group due to profound emotion regulation problems that impact on their behavior, sense of self and relationships with other people. Interactions with the Spheres are designed to motivate engagement with important therapeutic exercises of their specialist treatment Dialectical Behavioral Therapy (DBT), and to improve their quality of life and mental wellbeing more generally. This paper first briefly outlines the basic concept of the Spheres of Wellbeing, as they have been informed through a close, personfocused collaboration with hospital staff. We then describe in more detail our empathic approach to actively engage the women in creative activities that allow for a personalized design of the Spheres by each woman.

Co-Creating a Virtuous Cycle

Proceedings of the Participatory Design Conference 2022 - Volume 2

Out-casted/Re-casted: Towards a Lexicon for Restorative Artmaking and Co-creation

Art in Prisons: Talking Restorative Justice through Artistic and Narrative Projects, Sep 8, 2020

Clair Aldington’s doctoral research is broadly about the role of making, gifting and co-creation ... more Clair Aldington’s doctoral research is broadly about the role of making, gifting and co-creation within Restorative Justice processes. Within that, more narrowly, the research is focussing on Interaction Ritual as applied to Restorative Justice (Collins, 2004; Rossner, 2013) and whether or not a gifted co-created artefact could become an object symbol of solidarity, and in so doing, maintain the positive emotional energy generated by a Restorative Justice encounter. This paper highlights the complexities and confusion around the understanding and usage of the word “solidarity” in this particular context and offers a working research definition and understanding for its use within Restorative Justice. Additionally, the paper attempts to address the confusion of language around Restorative Justice, and suggests the need for a “Lexicon of Restorative Artmaking and Co-Creation” utilising words gathered with research participants. Drawing on initial findings from a research case study, it is posited that there is a potential role for the co-created artefact in changing the feelings of a participant in a Restorative Justice process from out-casted to re-casted within their community

The Significant Other

The significant other: the value of jewellery within the conception, design and experience of bod... more

Emotionally charged

A practice-centred enquiry of digital jewellery and personal emotional significance

How can we Support Meaningful Social Relationships through Digital Design

The possession of stable and supportive relationships has a crucial influence on a person’s emoti... more The possession of stable and supportive relationships has a crucial influence on a person’s emotional wellbeing. In order to inform the design of interactive technologies to meaningfully support the formation and maintenance of interpersonal attachments, this paper suggests a discussion on the qualities of social interactions and communications that allow for meaningfulness and wellbeing in peoples’ lives. Invited reflections on the potentials and limitations of digital designs targeted at nurturing social bonds relate to aspects of self-investment, privacy, intimacy and meaningfulness, which are rarely the focus of many existing social communication technologies.

Developing a Dialogical Platform for Disseminating Research through Design

Constructivist Foundations, 2015

Context: Practice-based design research is becoming more widely recognized in academia, including... more Context: Practice-based design research is becoming more widely recognized in academia, including at doctoral level, yet there are arguably limited options for dissemination beyond the traditional conference format of paper-based proceedings, possibly with an exhibition or “demonstrator” component that is often non-archival. Further, the opportunities afforded by the traditional-format paper presentations is at times at odds with practice-based methodologies being presented. Purpose: We provide a first-hand descriptive account of developing and running a new international conference with an experimental format that aims to support more analogously the dissemination of practice-based design research. Method: Our approach herein is broadly interpretative, phenomenological and critically reflective in orientation, to analyze our own experiential insights from the conference conception, through to the event itself and post-conference reflections, alongside the reflections fed back by co...

Unpicking the Digital

A series of four digital lockets exploring notions of memory, memory loss and our relationship to... more A series of four digital lockets exploring notions of memory, memory loss and our relationship to digital technologies. 'Remember' - This locket houses a large number of images, moving onto the next image each time the locket is opened. 'Forget' - When 'Forget' is opened the image inside degrades a small amount, the digital logic operates to hold onto the most precious amounts of an image for as long as possible, but eventually an image dissolves entirely and is lost. 'Daguerre' - This locket can hold only one image, taken with its connected camera, if a new image is taken it replaces the last. 'Orpheus' - This locket holds a single image taken with its camera, but it may only be seen twice; once on creation and once again when the locket is next opened, when, after a few seconds, the image fades and is deleted.

Staged Atmosphere: The Air[craft] Workshop

2013) have enabled valuable insights into people's lived experience and provoked unexpected desig... more 2013) have enabled valuable insights into people's lived experience and provoked unexpected design ideas that could enrich people's lives. They have been used to enrich the co-creative, empathetic context between a participant and a design researcher (Wallace et al., 2013). Making probes work can be challenging however (ibid.), and as documented by Gaver et al. (2004) and Boehner et al. (2007, 2012) the

Mozilla Design Sprint

As part of Mozilla’s exploration of the Internet of Things, we convened leading practitioners in ... more As part of Mozilla’s exploration of the Internet of Things, we convened leading practitioners in Anstruther, Scotland from Monday, June 20 - Friday, June 25, 2016 to respond to a clear design brief about how communities can be empowered collectively by using IoT and open practices. The Scottish design sprint was about actively involving communities to explore IoT and make technology alongside them so it fits their lives.

37 balancing openness and structure abigail durrant et al Constructivism

s of the siGCHi conference on human factors in computing systems. aCM Press, new York: 781–790. W... more s of the siGCHi conference on human factors in computing systems. aCM Press, new York: 781–790. Wolf T., Rode J., Sussman J. & Kellogg W. (2006) dispelling “design” as the black art of CHi. in: Proceedings of the siGCHi conference on human factors in computing systems. aCM Press, new York: 521–530. Yee J., Jefferies E. & Tan L. (2013) design transitions: inspiring stories. Global viewpoints. How design is changing. Bis, amsterdam. Zimmerman J., Stolterman E. & Forlizzi J. (2010) an analysis and critique of research through design. in: Proceedings of the 8th aCM Conference on designing interactive systems (dis’10). aCM Press, aarhus

Highly parallel direct RNA sequencing on an array of nanopores

Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us... more Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing – information that is useful for understanding the status and function of a sample. Oxford Nanopore Technologies’ sequencing technology is capable of electronically analysing a sample’s DNA directly, and in real-time. In this manuscript we demonstrate the ability of an array of nanopores to sequence RNA directly, and we apply it to a range of biological situations. Nanopore technology is the only available sequencing technology that can sequence RNA directly, rather than depending on reverse transcription and PCR. There are several potential advantages of this approach over other RNA-seq strategies, including the absence of amplification and reverse transcription biases, the ability to detect nucleotide analogues and the ability to generate full-length, s...

Crystal structure of an invertebrate cytolysin pore reveals unique properties and mechanism of assembly

Nature communications, May 12, 2016

The invertebrate cytolysin lysenin is a member of the aerolysin family of pore-forming toxins tha... more The invertebrate cytolysin lysenin is a member of the aerolysin family of pore-forming toxins that includes many representatives from pathogenic bacteria. Here we report the crystal structure of the lysenin pore and provide insights into its assembly mechanism. The lysenin pore is assembled from nine monomers via dramatic reorganization of almost half of the monomeric subunit structure leading to a β-barrel pore ∼10 nm long and 1.6-2.5 nm wide. The lysenin pore is devoid of additional luminal compartments as commonly found in other toxin pores. Mutagenic analysis and atomic force microscopy imaging, together with these structural insights, suggest a mechanism for pore assembly for lysenin. These insights are relevant to the understanding of pore formation by other aerolysin-like pore-forming toxins, which often represent crucial virulence factors in bacteria.

Free-Energy Calculations Reveal the Subtle Differences in the Interactions of DNA Bases with α-Hemolysin

Journal of Chemical Theory and Computation, 2015

Next generation DNA sequencing methods that utilize protein nanopores have the potential to revol... more Next generation DNA sequencing methods that utilize protein nanopores have the potential to revolutionize this area of biotechnology. While the technique is underpinned by simple physics, the wild-type protein pores do not have all of the desired properties for efficient and accurate DNA sequencing. Much of the research efforts have focused on protein nanopores, such as α-hemolysin from Staphylococcus aureus. However, the speed of DNA translocation has historically been an issue, hampered in part by incomplete knowledge of the energetics of translocation. Here we have utilized atomistic molecular dynamics simulations of nucleotide fragments in order to calculate the potential of mean force (PMF) through α-hemolysin. Our results reveal specific regions within the pore that play a key role in the interaction with DNA. In particular, charged residues such as D127 and K131 provide stabilizing interactions with the anionic DNA and therefore are likely to reduce the speed of translocation. These regions provide rational targets for pore optimization. Furthermore, we show that the energetic contributions to the protein−DNA interactions are a complex combination of electrostatics and short-range interactions, often mediated by water molecules.

Barriers to patient service innovation in the UK National Health Service (NHS): A case study of seeking permission to conduct service design research for long term conditions, specifically Type 1 diabetes

Size and Number of Epithelial Cells Washed from the Cornea after Contact Lens Wear

Optometry and Vision Science, 1998

Carbon nanotube self-assembly with lipids and detergent: a molecular dynamics study

Nanotechnology, 2008

The dispersion of carbon nanotubes (CNTs) in aqueous media is of potential importance in a number... more The dispersion of carbon nanotubes (CNTs) in aqueous media is of potential importance in a number of biomedical applications. CNT solubilization has been achieved via the non-covalent adsorption of lipids and detergent onto the tube surface. We use coarse-grained molecular dynamics to study the self-assembly of CNTs with various amphiphiles, namely a bilayer-forming lipid, dipalmitoylphosphatidylcholine (DPPC), and two species of detergent, dihexanoylphosphatidylcholine (DHPC) and lysophosphatidylcholine (LPC). We find that for a low amphiphile/CNT ratio, DPPC, DHPC and LPC all wrap around the CNT. Upon increasing the number of amphiphiles, a transition in adsorption is observed: DPPC encapsulates the CNT within a cylindrical micelle, whilst both DHPC and LPC adsorb onto CNTs in hemimicelles. This study highlights differences in adsorption mechanism of bilayer-forming lipids and detergents on CNTs which may in the future be exploitable to enable enhancement of CNT solubilization whilst minimizing perturbation of cell membrane integrity.

Carbon nanotube/detergent interactions via coarse-grained molecular dynamics

Chemistry and Physics of Lipids, 2007

ReFind: Design, Lived Experience and Ongoingness in Bereavement

We describe the design and use of ReFind, a handheld artefact made for people who are bereaved an... more We describe the design and use of ReFind, a handheld artefact made for people who are bereaved and are ready to re-explore their relationship to the deceased person. ReFind was made within a project seeking to develop new ways to curate and create digital media to support ongoingness-an active, dynamic component of continuing bonds. We draw on bereavement theory and care championing practices that enable a continued sense of connection between someone bereaved and a person who has died. We present the design development of ReFind and the lived experience of the piece by the first author. We discuss our wider methodology which includes autobiographical design and reflections on if and how the piece supported ongoing connections, the challenges faced, and insights gained.

Empathetic Design for and with Vulnerable Women in Secure Hospital Services

We present our work in progress on the design process of a set of interactive objects, the Sphere... more We present our work in progress on the design process of a set of interactive objects, the Spheres of Wellbeing, for and with women in secure hospital services. The women present a very vulnerable and difficult-to-treat group due to profound emotion regulation problems that impact on their behavior, sense of self and relationships with other people. Interactions with the Spheres are designed to motivate engagement with important therapeutic exercises of their specialist treatment Dialectical Behavioral Therapy (DBT), and to improve their quality of life and mental wellbeing more generally. This paper first briefly outlines the basic concept of the Spheres of Wellbeing, as they have been informed through a close, personfocused collaboration with hospital staff. We then describe in more detail our empathic approach to actively engage the women in creative activities that allow for a personalized design of the Spheres by each woman.

Co-Creating a Virtuous Cycle

Proceedings of the Participatory Design Conference 2022 - Volume 2

Out-casted/Re-casted: Towards a Lexicon for Restorative Artmaking and Co-creation

Art in Prisons: Talking Restorative Justice through Artistic and Narrative Projects, Sep 8, 2020

Clair Aldington’s doctoral research is broadly about the role of making, gifting and co-creation ... more Clair Aldington’s doctoral research is broadly about the role of making, gifting and co-creation within Restorative Justice processes. Within that, more narrowly, the research is focussing on Interaction Ritual as applied to Restorative Justice (Collins, 2004; Rossner, 2013) and whether or not a gifted co-created artefact could become an object symbol of solidarity, and in so doing, maintain the positive emotional energy generated by a Restorative Justice encounter. This paper highlights the complexities and confusion around the understanding and usage of the word “solidarity” in this particular context and offers a working research definition and understanding for its use within Restorative Justice. Additionally, the paper attempts to address the confusion of language around Restorative Justice, and suggests the need for a “Lexicon of Restorative Artmaking and Co-Creation” utilising words gathered with research participants. Drawing on initial findings from a research case study, it is posited that there is a potential role for the co-created artefact in changing the feelings of a participant in a Restorative Justice process from out-casted to re-casted within their community

The Significant Other

The significant other: the value of jewellery within the conception, design and experience of bod... more

Emotionally charged

A practice-centred enquiry of digital jewellery and personal emotional significance

How can we Support Meaningful Social Relationships through Digital Design

The possession of stable and supportive relationships has a crucial influence on a person’s emoti... more The possession of stable and supportive relationships has a crucial influence on a person’s emotional wellbeing. In order to inform the design of interactive technologies to meaningfully support the formation and maintenance of interpersonal attachments, this paper suggests a discussion on the qualities of social interactions and communications that allow for meaningfulness and wellbeing in peoples’ lives. Invited reflections on the potentials and limitations of digital designs targeted at nurturing social bonds relate to aspects of self-investment, privacy, intimacy and meaningfulness, which are rarely the focus of many existing social communication technologies.

Developing a Dialogical Platform for Disseminating Research through Design

Constructivist Foundations, 2015

Context: Practice-based design research is becoming more widely recognized in academia, including... more Context: Practice-based design research is becoming more widely recognized in academia, including at doctoral level, yet there are arguably limited options for dissemination beyond the traditional conference format of paper-based proceedings, possibly with an exhibition or “demonstrator” component that is often non-archival. Further, the opportunities afforded by the traditional-format paper presentations is at times at odds with practice-based methodologies being presented. Purpose: We provide a first-hand descriptive account of developing and running a new international conference with an experimental format that aims to support more analogously the dissemination of practice-based design research. Method: Our approach herein is broadly interpretative, phenomenological and critically reflective in orientation, to analyze our own experiential insights from the conference conception, through to the event itself and post-conference reflections, alongside the reflections fed back by co...

Unpicking the Digital

A series of four digital lockets exploring notions of memory, memory loss and our relationship to... more A series of four digital lockets exploring notions of memory, memory loss and our relationship to digital technologies. 'Remember' - This locket houses a large number of images, moving onto the next image each time the locket is opened. 'Forget' - When 'Forget' is opened the image inside degrades a small amount, the digital logic operates to hold onto the most precious amounts of an image for as long as possible, but eventually an image dissolves entirely and is lost. 'Daguerre' - This locket can hold only one image, taken with its connected camera, if a new image is taken it replaces the last. 'Orpheus' - This locket holds a single image taken with its camera, but it may only be seen twice; once on creation and once again when the locket is next opened, when, after a few seconds, the image fades and is deleted.

Staged Atmosphere: The Air[craft] Workshop

2013) have enabled valuable insights into people's lived experience and provoked unexpected desig... more 2013) have enabled valuable insights into people's lived experience and provoked unexpected design ideas that could enrich people's lives. They have been used to enrich the co-creative, empathetic context between a participant and a design researcher (Wallace et al., 2013). Making probes work can be challenging however (ibid.), and as documented by Gaver et al. (2004) and Boehner et al. (2007, 2012) the

Mozilla Design Sprint

As part of Mozilla’s exploration of the Internet of Things, we convened leading practitioners in ... more As part of Mozilla’s exploration of the Internet of Things, we convened leading practitioners in Anstruther, Scotland from Monday, June 20 - Friday, June 25, 2016 to respond to a clear design brief about how communities can be empowered collectively by using IoT and open practices. The Scottish design sprint was about actively involving communities to explore IoT and make technology alongside them so it fits their lives.

37 balancing openness and structure abigail durrant et al Constructivism

s of the siGCHi conference on human factors in computing systems. aCM Press, new York: 781–790. W... more s of the siGCHi conference on human factors in computing systems. aCM Press, new York: 781–790. Wolf T., Rode J., Sussman J. & Kellogg W. (2006) dispelling “design” as the black art of CHi. in: Proceedings of the siGCHi conference on human factors in computing systems. aCM Press, new York: 521–530. Yee J., Jefferies E. & Tan L. (2013) design transitions: inspiring stories. Global viewpoints. How design is changing. Bis, amsterdam. Zimmerman J., Stolterman E. & Forlizzi J. (2010) an analysis and critique of research through design. in: Proceedings of the 8th aCM Conference on designing interactive systems (dis’10). aCM Press, aarhus