Turk Pediatri Arsivi-turkish Archives of Pediatrics, Dec 15, 2010
Y Ye en ni id do o¤ ¤a an nd da a v vo ol lü üm m g ga ar ra an nt ti il li i v ve en nt ti il la... more Y Ye en ni id do o¤ ¤a an nd da a v vo ol lü üm m g ga ar ra an nt ti il li i v ve en nt ti il la as sy yo on n i il le e b ba as s› ›n nç ç k ko on nt tr ro ol ll lü ü v ve en nt ti il la as sy yo on nu un n k ka ar rfl fl› ›l la afl flt t› ›r r› ›l lm ma as s› › V Vo ol lu um me e g gu ua ar ra an nt te ee e v ve en nt ti il la at ti io on n v ve er rs su us s p pr re es ss su ur re e l li im mi it t v ve en nt ti il la at ti io on n i
Journal of Clinical Research in Pediatric Endocrinology, Mar 5, 2015
Thanatophoric dysplasia (TD) is a lethal form of skeletal dysplasia with shortlimb dwarfism. Two ... more Thanatophoric dysplasia (TD) is a lethal form of skeletal dysplasia with shortlimb dwarfism. Two types distinguished with their radiological characteristics have been defined clinically. The femur is curved in type 1, while it is straight in type 2. TD is known to be due to a mutation in the fibroblast growth factor receptor 3 (FGFR3) gene. We report a male patient who showed clinical findings congruent with TD type 2 and a new mutation in the FGFR3 gene, a finding which has not been reported previously.
Mersin üniversitesi sağlık bilimleri dergisi, Apr 15, 2018
Amaç: Doğum ağırlığı 1 500 g altında olan çok düşük doğum ağırlıklı (ÇDDA) bebeklerin uzun dönem ... more Amaç: Doğum ağırlığı 1 500 g altında olan çok düşük doğum ağırlıklı (ÇDDA) bebeklerin uzun dönem izlem sonucunda nöro-gelişimsel özelliklerinin belirlenmesi amaçlandı. Yöntem: 2002-2009 yılları arasında 1500 g altında doğan, doğum ağırlıkları gebelik yaşlarıyla uyumlu ve merkezimizde Yenidoğan Yoğun Bakım Ünitesi'nde tedavi edilen 62 çok düşük doğum ağırlıklı erken doğum öyküsüne sahip çocuklar çalışmaya alındı. ÇDDA'lı çocuklara yaşlarına uygun olan gelişimsel testler (Denver II Gelişimsel Tarama Testi, Bayley Bebeklik ve Erken Çocukluk Dönemi Gelişimsel Değerlendirme Ölçeği III) ve Stanford Binet Zekâ Ölçeği uygulandı. Kontrol grubuna yaş ve cinsiyetleri ÇDDA'lı çocuklarla uyumlu, normal doğum ağırlıklı, zamanında doğmuş çocuklar alınarak bu iki grup birbirleriyle karşılaştırıldı. Bulgular: Bayley III, Denver Gelişimsel Tarama Testi ve Stanford Binet Zekâ Ölçeği'nin tamamında ÇDDA'lı çocukların kontrol grubuna göre anlamlı olarak geri oldukları saptandı. Vajinal yolla doğum sonucunda beyin felci gelişme oranının sezaryen doğuma göre anlamlı olarak yüksek olduğu saptandı (p=0.003). Sepsis tanısı alan ÇDDA'lı çocuklarda Denver Gelişimsel Tarama Testi'nde anormallik oranının daha yüksek olduğu saptandı (p=0.023). Sonuç: ÇDDA'lı erken doğanlarda ileri dönemlerde gelişimsel sorunlar yüksek oranda görüldüğünden, bu bebeklerin uzun süreli izlemleri ve desteklenmeleri gereklidir. Sepsisin nörolojik gelişim üzerine yıkıcı etkileri net olarak görüldüğünden bu bebeklerin sepsisten korunmasına özel önem gösterilmelidir.
Two cases with macroscopic hematuria as complication of acute mumps infection is reported. The pa... more Two cases with macroscopic hematuria as complication of acute mumps infection is reported. The patients have neither been vaccinated against mumps nor had mumps infection earlier. Macroscopic hematuria resolved spontaneously and renal functions did not deteriorate in both the patients. Although mumps has a benign course, mild and rarely severe renal involvement may occur. Therefore, renal functions in patients with hematuria and mumps should be followed closely.
Journal of Maternal-fetal & Neonatal Medicine, Sep 12, 2015
This study aimed to determine the effect of pluripotent astrocytic stem cells (PASCs) and fibrobl... more This study aimed to determine the effect of pluripotent astrocytic stem cells (PASCs) and fibroblast growth factor-2 (FGF-2) on cognitive function in neonatal rats with hypoxic-ischemic brain injury (HIBI). The study was performed on 7-d-old rats that were randomly divided into four groups. All rats, except those in the sham group, were kept in a hypoxic chamber containing 8% oxygen for 2 h after the ligation of the right carotid artery. Next, 5 d after HIBI was induced, PASCs were administered to the motor cortex, and FGF-2 was administered intraperitoneally to group AF; PASCs were administered to the motor cortex, and salt solution buffered with phosphate was administered intraperitoneally to group A; and fresh cell culture solution (medium) was administered to group M. Immunofluorescence was used to localize the administered PASCs in the brains of rats from groups A and AF. The Morris water maze tank (MWM) test was performed to assess the rats' cognitive functions at week 12. The rats that were administered PASCs were observed for the development of neoplasms and autopsies were performed after 30 months. PASCs migrated to damaged brain regions surrounding the hippocampus in groups A and AF. The mean platform finding time (PFT) significantly decreased over time in each group on day 1-4 of MWM testing (p < 0.001). On day 2-4, the mean PFT was shortest in group S followed by group AF. In group A, the PFT was significantly longer than in group S on day 3-4 (p = 0.01 and 0.007, respectively). On day 5 of the MWM test, the time spent in the eastern quadrant (which previously contained the platform) was longest in group S followed by groups AF, A, and M; however, the differences between groups were not significant (p = 0.51). After 30 months, none of the rats in groups A or AF had benign or malignant neoplasms. Following the administration of PASCs in rats with experimentally induced HIBI, PASCs migrated to the injured brain regions; however, treatment with PASCs did not have a positive effect on cognitive function. The administration of FGF-2 together with PASCs resulted in positive cognitive results, although not at the level of significance.
Turk Pediatri Arsivi-turkish Archives of Pediatrics, Mar 15, 2012
Türk Pe di at ri Ar şi vi Der gi si, Ga le nos Ya yı ne vi ta ra fın dan ba sıl mış tır. / Tur ki... more Türk Pe di at ri Ar şi vi Der gi si, Ga le nos Ya yı ne vi ta ra fın dan ba sıl mış tır. / Tur kish Arc hi ves of Pe di at rics, pub lis hed by Ga le nos Pub lis hing Yenidoğanda yüksek hızda titreşimli ventilasyon: üç yıllık deneyimlerimiz High-frequency oscillatory ventilation in newborn: three years of experience Ya z›fl ma Ad re si/Ad dress for Cor res pon den ce:
To evaluate effectiveness of tracheally delivered mesenchymal stem cells (MSCs) on lung pathology... more To evaluate effectiveness of tracheally delivered mesenchymal stem cells (MSCs) on lung pathology in hyperoxia-induced lung injury (HILI) model in neonatal rats. For HILI model, rat pups were exposed to 85-95% oxygen during the first 10 days of life. Rats were divided into six groups: (1) room-air normoxia (n = 11); (2) room-air, sham (n = 11); (3) hyperoxia-exposed / normal saline as placebo (n = 9); (4) hyperoxia-exposed/culture medium of MSCs(n = 10); (5) hyperoxia-exposed/ medium remaining after harvesting of MSCs (n = 8); and (6) hyperoxia-exposed/ MSCs (n = 17). Pathologic changes, number and diameter of alveoli, α-smooth muscle actin expression and localization of MSCs in the lungs were assessed. Number of alveoli increased and alveolar diameter decreased in the mesenchymal stem cell group so that there was no differences when compared with the normoxia group (respectively p = 0.126 and p = 0.715). Expression of α-smooth muscle actin decreased significantly in the mesenchymal stem cell group compared with the placebo group…
Turk Pediatri Arsivi-turkish Archives of Pediatrics, Apr 10, 2015
Bu çalışmada orta ya da ağır hipoksik iskemik ansefalopati tanısı alan yenidoğan bebeklere seçici... more Bu çalışmada orta ya da ağır hipoksik iskemik ansefalopati tanısı alan yenidoğan bebeklere seçici baş soğutma ya da tüm beden soğutma tedavisi uygulayarak hangi yöntemin daha üstün olduğunun araştırılması amaçlandı. Gereç ve Yöntemler: Çalışmaya 35. gebelik haftasından büyük, orta ya da ağır hipoksik iskemik ansefalopati tanısı alan yenidoğanlar alındı ve rastlantısal olarak seçici baş soğutma ya da tüm beden soğutma tedavisi uygulandı. Her iki yöntemle soğutma tedavisi uygulanan yenidoğanlar erken dönemde istenmeyen etkiler ve kısa dönem sonuçları açısından karşılaştırıldı. Çalışma için etik kurul izni alındı (06.01.2010/35). Bulgular: Hipoksik iskemik ansefalopati tanısı alan 53 bebek incelendi ve 17 bebeğe seçici baş soğutma, 12 bebeğe tüm beden soğutma uygulandı. Soğutma tedavisine bağlı olarak gelişebilecek istenmeyen etkiler açısından iki grup arasında fark bulunmadı. Erken dönem sonuçları incelendiğinde hastanede yatış süresi seçici baş soğutma grubunda 34 (7-65) gün iken, tüm beden soğutma grubunda 18 (7-57) gün idi ve aralarında anlamlı fark yoktu (p=0,097). Seçici baş soğutma grubundan dört, tüm beden soğutma grubundan iki hasta uzamış mekanik ventilasyon gereksinimi nedeniyle trakeostomi ile taburcu edildiler ve trakeostomi ile taburculuk açısından gruplar arasında fark yoktu (p=0,528). Seçici baş soğutma grubundan beş, tüm beden soğutma grubundan üç hasta ağızdan beslenememeleri nedeniyle gastrostomi tüpü ile taburcu edildiler ve gastrostomi tüpü ile taburculuk açısından gruplar arasında fark yoktu (p=0,586). Seçici baş soğutma tedavisi uygulanan bebeklerden biri, tüm beden soğutma uygulanan bebeklerden biri hastanede yaşamını kaybetti ve ölüm açısından gruplar arasında fark yoktu (p=0,665). Çıkarımlar: Seçici baş soğutma ve tüm beden soğutma yöntemleri arasında istenmeyen etkiler ve kısa dönem sonuçları açısından fark yoktur.
Hypoxic-ischemic brain injury that occurs in the perinatal period is one of the leading causes of... more Hypoxic-ischemic brain injury that occurs in the perinatal period is one of the leading causes of mental retardation, visual and auditory impairment, motor defects, epilepsy, cerebral palsy, and death in neonates. The severity of apoptosis that develops after ischemic hypoxia and reperfusion is an indication of brain injury. Thus, it may be possible to prevent or reduce injury with treatments that can be given before the reperfusion period following hypoxia and ischemia. Levetiracetam is a new-generation antiepileptic drug that has begun to be used in the treatment of epilepsy. The present study investigated the effects of levetiracetam on neuronal apoptosis with histopathological and biochemical tests in the early period and behavioral experiments in the late period. This study showed histopathologically that levetiracetam reduces the number of apoptotic neurons and has a neuroprotective effect in a neonatal rat model of hypoxic-ischemic brain injury in the early period. On the other hand, we demonstrated that levetiracetam dose dependently improves behavioral performance in the late period. Based on these results, we believe that one mechanism of levetiracetam's neuroprotective effects is due to increases in glutathione peroxidase and superoxide dismutase enzyme levels. To the best of our knowledge, this study is the first to show the neuroprotective effects of levetiracetam in a neonatal rat model of hypoxic-ischemic brain injury using histopathological, biochemical, and late-period behavioral experiments within the same experimental group.
BACKGROUND: The aim of this study was to determine if levetiracetam (LEV) is neuroprotective in n... more BACKGROUND: The aim of this study was to determine if levetiracetam (LEV) is neuroprotective in neonatal rats with hypoxic-ischemic brain injury (HIBI). METHODS: The study included 7-d-old male Wistar rats that were randomly divided into the LEV400, LEV800, control, and sham groups. All the rats, except those in the sham group, underwent ligation of the carotid artery and were then kept in a hypoxic chamber containing 8% oxygen for 2 h. At the end of the hypoxic period the rats in the control group were administered saline solution 0.5 mL, the rats in the LEV400 group were administered LEV 400 mg.kg-1 , and rats in the LEV800 group were administered LEV 800 mg.kg-1 via the intraperitoneal route. The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) method was used to evaluate neuronal apoptosis in the rats. The Morris Water Maze (MWM) test was performed at age 14 weeks in order to evaluate cognitive function. RESULTS: The number of apoptotic neurons in the right hemispheres was signifi cantly lower in the sham, LEV400, and LEV800 groups than in the control group (p < 0.001, p < 0.001, and p < 0.001, respectively). In addition, the number of apoptotic neurons in the right hemispheres was signifi cantly lower in the LEV800 group than in the LEV400 group (p = 0.001). Platform fi nding time (PFT) during MWM testing was signifi cantly shorter in the sham and LEV800 groups on d 4 than on d 1 (p = 0.001 and p = 0.006, respectively); however, PFT did not signifi cantly change between d 1 and d 4 in the control or LEV400 groups (p = 0.91 and p = 0.096, respectively). CONCLUSION: Based on the present fi ndings, LEV exhibited a dose-dependent neuroprotective effect in neonatal rats with HIBI (Ref. 27).
Turk Pediatri Arsivi-turkish Archives of Pediatrics, Jul 20, 2015
Ectopia cordis is a rare congenital malformation in which the heart is located partially or total... more Ectopia cordis is a rare congenital malformation in which the heart is located partially or totally outside the thoracic cavity. The estimated prevalence of ectopia cordis is 5.5-7.9 per million births and it comprises 0.1% of congenital heart diseases. Ectopia cordis is associated with other congenital heart diseases and various tissue and organ disorders. Common cardiac anomalies associated with ectopia cordis include ventricular septal defect, atrial septal defect, pulmonary stenosis, right ventricular diverticulum, double right ventricular outflow tract and tetralogy of Fallot. Extracardiac anomalies associated with ectopia cordis reported in the literature include omphalocele, gastrochisis, cleft lip and palate, scollosis and central nervous system malformations. Here we report a newborn with ectopia cordis who was diagnosed prenatally.
Özofagus atrezisi ve trakeoözofageal fistül nedeni ile kliniğimizde tedavi edilmiş hastalarda uyg... more Özofagus atrezisi ve trakeoözofageal fistül nedeni ile kliniğimizde tedavi edilmiş hastalarda uyguladığımız cerrahi yöntemler ve tedavi sonuçlarını irdelemektir.
Johanson Blizzard Sendromu (JBS) ender görülen; burun kanatlarının yokluğu veya az gelişmişliği, ... more Johanson Blizzard Sendromu (JBS) ender görülen; burun kanatlarının yokluğu veya az gelişmişliği, dişte yapısal bozukluklar, orta hat deri defektleri, büyüme ve zekâ geriliği, pankreas yetmezliği, anüsün doğuştan kapalı olması ve doğumsal sağırlıkla kendini gösteren bir durumdur. Otozomal resesif kalıtılır. Anüsün kapalı olması nedeniyle getirilen ve burun kanadında az gelişmişlik saptanan vakada JBS düşünülmüş ve ender rastlanması nedeniyle sunulmuştur.
Jeune sendromu asfiktik torasik distrofi olarak da isimlendirilen otozomal resesif geçişli çoklu ... more Jeune sendromu asfiktik torasik distrofi olarak da isimlendirilen otozomal resesif geçişli çoklu organ tutulumu olan bir iskelet displazisidir. Dar, çan şeklinde göğüs duvarı, farklı derecelerde kol ve bacak kısalığı bu sendromun en karakteristik özelliğidir. Jeune sendromlu çocukların çoğunda erken süt çocukluğu döneminde ağır solunum yetmezliği görülür. Burada doğumun hemen ardından solunum yetmezliği gelişen ve Jeune sendromu tanısı alan bir olgu nadir görülmesi nedeniyle sunulmuştur. Akraba evliliklerinin sık olduğu ülkemizde solunum sıkıntısı bulgularının yanında dar göğüs kafesi, kısa kol ve bacakların görüldüğü bebeklerde Jeune sendromu akla gelmelidir. AnAhtAr sözcüKler: Jeune sendromu, asfiktik torasik distrofi, yenidoğan Jeune syndrome, also known as asphyxiating thoracic dystrophy, is a rare autosomal recessive skeletal dysplasia with multiorgan involvement. Clinically, Jeune syndrome is characterised by a bell-shaped, narrow chest and variable limb shortness. Most of the children suffering from this syndrome present with severe respiratory failure during the early infancy period. Here we report a neonate with serious postnatal respiratory insufficiency who was diagnosed with Jeune syndrome. Since intermarriage is frequent in Turkey, when a newborn with respiratory failure has a bell-shaped, narrow chest and variable limb shortness, Jeune syndrome should be considered.
Accessory nostril is a very rare congenital anomaly with an unknown etiology also known as supern... more Accessory nostril is a very rare congenital anomaly with an unknown etiology also known as supernumerary nostril. A few accessory nostrils have been reported up to the present time, and extremely rare cases located on columella. A newborn infant with respiratory distress was referred to our hospital. The authors observed that accessory nasal nostril is not related to normal nasal cavity on the median line of columella. In this article, the authors reported accessory nostril case in newborn and review the literature.
Background: The aim of the study is to predict prognosis of newborns with congenital diaphragmati... more Background: The aim of the study is to predict prognosis of newborns with congenital diaphragmatic hernia (CDH) with the left ventricular diameter and estimated pulmonary artery pressure values. Methods: Patients in the newborn unit in 2012-2016 were screened retrospectively. Echocardiographic measurements of 35 patients with isolated left lateral CDH and 25 healthy newborn were compared in the first 24h of life. Results: The mean values of left ventricular end-diastolic (LVEDD) and end-systolic (LVESD) diameters, and ejection fraction were significantly lower in the patients. Moreover, tricuspid insufficiency (TR) and pulmonary insufficiency (PR) were significantly higher than controls (p<0.001). Six patients died within the next 40 days after birth. Mean values of LVEDD and LVESD were significantly lower in patients who died when compared to discharged (p<0.001, p=0.016). Also, mean values of TR and PR (p<0.001) and the frequency of pulmonary hypertension (PPH) (p=0.001) were significantly higher in these patients. In the ROC analysis of the patients who died LVEDD<11 mm [sensitivity 100% (95% CI:87.9-100) and specificity 100% (95% CI:54.1-100) and TR>3.5m/s [sensitivity 89.66% (95% CI:72.6-97.7) and specificity 100% (95% CI:54.1-100)] were demonstrated to be associated with poor prognosis. Conclusions: In this study, decrease in LV diameter and presence of pulmonary hypertension was shown to be associated with poor prognosis. Also, presence of PPH was found to be associated with mortality in patients with CDH. Thus, it is assumed that, in the follow-up prognosis may be predicted by a carefully echocardiographic evaluation of the LV diameters and pulmonary pressures.
Acta Orthopaedica et Traumatologica Turcica, Mar 5, 2020
The role of the platelet-to-lymphocyte ratio and neutrophil-to-lymphocyte ratio in the prediction... more The role of the platelet-to-lymphocyte ratio and neutrophil-to-lymphocyte ratio in the prediction of length and cost of hospital stay in patients with infected diabetic foot ulcers: A retrospective comparative study
Turk Pediatri Arsivi-turkish Archives of Pediatrics, Dec 15, 2010
Y Ye en ni id do o¤ ¤a an nd da a v vo ol lü üm m g ga ar ra an nt ti il li i v ve en nt ti il la... more Y Ye en ni id do o¤ ¤a an nd da a v vo ol lü üm m g ga ar ra an nt ti il li i v ve en nt ti il la as sy yo on n i il le e b ba as s› ›n nç ç k ko on nt tr ro ol ll lü ü v ve en nt ti il la as sy yo on nu un n k ka ar rfl fl› ›l la afl flt t› ›r r› ›l lm ma as s› › V Vo ol lu um me e g gu ua ar ra an nt te ee e v ve en nt ti il la at ti io on n v ve er rs su us s p pr re es ss su ur re e l li im mi it t v ve en nt ti il la at ti io on n i
Journal of Clinical Research in Pediatric Endocrinology, Mar 5, 2015
Thanatophoric dysplasia (TD) is a lethal form of skeletal dysplasia with shortlimb dwarfism. Two ... more Thanatophoric dysplasia (TD) is a lethal form of skeletal dysplasia with shortlimb dwarfism. Two types distinguished with their radiological characteristics have been defined clinically. The femur is curved in type 1, while it is straight in type 2. TD is known to be due to a mutation in the fibroblast growth factor receptor 3 (FGFR3) gene. We report a male patient who showed clinical findings congruent with TD type 2 and a new mutation in the FGFR3 gene, a finding which has not been reported previously.
Mersin üniversitesi sağlık bilimleri dergisi, Apr 15, 2018
Amaç: Doğum ağırlığı 1 500 g altında olan çok düşük doğum ağırlıklı (ÇDDA) bebeklerin uzun dönem ... more Amaç: Doğum ağırlığı 1 500 g altında olan çok düşük doğum ağırlıklı (ÇDDA) bebeklerin uzun dönem izlem sonucunda nöro-gelişimsel özelliklerinin belirlenmesi amaçlandı. Yöntem: 2002-2009 yılları arasında 1500 g altında doğan, doğum ağırlıkları gebelik yaşlarıyla uyumlu ve merkezimizde Yenidoğan Yoğun Bakım Ünitesi'nde tedavi edilen 62 çok düşük doğum ağırlıklı erken doğum öyküsüne sahip çocuklar çalışmaya alındı. ÇDDA'lı çocuklara yaşlarına uygun olan gelişimsel testler (Denver II Gelişimsel Tarama Testi, Bayley Bebeklik ve Erken Çocukluk Dönemi Gelişimsel Değerlendirme Ölçeği III) ve Stanford Binet Zekâ Ölçeği uygulandı. Kontrol grubuna yaş ve cinsiyetleri ÇDDA'lı çocuklarla uyumlu, normal doğum ağırlıklı, zamanında doğmuş çocuklar alınarak bu iki grup birbirleriyle karşılaştırıldı. Bulgular: Bayley III, Denver Gelişimsel Tarama Testi ve Stanford Binet Zekâ Ölçeği'nin tamamında ÇDDA'lı çocukların kontrol grubuna göre anlamlı olarak geri oldukları saptandı. Vajinal yolla doğum sonucunda beyin felci gelişme oranının sezaryen doğuma göre anlamlı olarak yüksek olduğu saptandı (p=0.003). Sepsis tanısı alan ÇDDA'lı çocuklarda Denver Gelişimsel Tarama Testi'nde anormallik oranının daha yüksek olduğu saptandı (p=0.023). Sonuç: ÇDDA'lı erken doğanlarda ileri dönemlerde gelişimsel sorunlar yüksek oranda görüldüğünden, bu bebeklerin uzun süreli izlemleri ve desteklenmeleri gereklidir. Sepsisin nörolojik gelişim üzerine yıkıcı etkileri net olarak görüldüğünden bu bebeklerin sepsisten korunmasına özel önem gösterilmelidir.
Two cases with macroscopic hematuria as complication of acute mumps infection is reported. The pa... more Two cases with macroscopic hematuria as complication of acute mumps infection is reported. The patients have neither been vaccinated against mumps nor had mumps infection earlier. Macroscopic hematuria resolved spontaneously and renal functions did not deteriorate in both the patients. Although mumps has a benign course, mild and rarely severe renal involvement may occur. Therefore, renal functions in patients with hematuria and mumps should be followed closely.
Journal of Maternal-fetal & Neonatal Medicine, Sep 12, 2015
This study aimed to determine the effect of pluripotent astrocytic stem cells (PASCs) and fibrobl... more This study aimed to determine the effect of pluripotent astrocytic stem cells (PASCs) and fibroblast growth factor-2 (FGF-2) on cognitive function in neonatal rats with hypoxic-ischemic brain injury (HIBI). The study was performed on 7-d-old rats that were randomly divided into four groups. All rats, except those in the sham group, were kept in a hypoxic chamber containing 8% oxygen for 2 h after the ligation of the right carotid artery. Next, 5 d after HIBI was induced, PASCs were administered to the motor cortex, and FGF-2 was administered intraperitoneally to group AF; PASCs were administered to the motor cortex, and salt solution buffered with phosphate was administered intraperitoneally to group A; and fresh cell culture solution (medium) was administered to group M. Immunofluorescence was used to localize the administered PASCs in the brains of rats from groups A and AF. The Morris water maze tank (MWM) test was performed to assess the rats&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; cognitive functions at week 12. The rats that were administered PASCs were observed for the development of neoplasms and autopsies were performed after 30 months. PASCs migrated to damaged brain regions surrounding the hippocampus in groups A and AF. The mean platform finding time (PFT) significantly decreased over time in each group on day 1-4 of MWM testing (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). On day 2-4, the mean PFT was shortest in group S followed by group AF. In group A, the PFT was significantly longer than in group S on day 3-4 (p = 0.01 and 0.007, respectively). On day 5 of the MWM test, the time spent in the eastern quadrant (which previously contained the platform) was longest in group S followed by groups AF, A, and M; however, the differences between groups were not significant (p = 0.51). After 30 months, none of the rats in groups A or AF had benign or malignant neoplasms. Following the administration of PASCs in rats with experimentally induced HIBI, PASCs migrated to the injured brain regions; however, treatment with PASCs did not have a positive effect on cognitive function. The administration of FGF-2 together with PASCs resulted in positive cognitive results, although not at the level of significance.
Turk Pediatri Arsivi-turkish Archives of Pediatrics, Mar 15, 2012
Türk Pe di at ri Ar şi vi Der gi si, Ga le nos Ya yı ne vi ta ra fın dan ba sıl mış tır. / Tur ki... more Türk Pe di at ri Ar şi vi Der gi si, Ga le nos Ya yı ne vi ta ra fın dan ba sıl mış tır. / Tur kish Arc hi ves of Pe di at rics, pub lis hed by Ga le nos Pub lis hing Yenidoğanda yüksek hızda titreşimli ventilasyon: üç yıllık deneyimlerimiz High-frequency oscillatory ventilation in newborn: three years of experience Ya z›fl ma Ad re si/Ad dress for Cor res pon den ce:
To evaluate effectiveness of tracheally delivered mesenchymal stem cells (MSCs) on lung pathology... more To evaluate effectiveness of tracheally delivered mesenchymal stem cells (MSCs) on lung pathology in hyperoxia-induced lung injury (HILI) model in neonatal rats. For HILI model, rat pups were exposed to 85-95% oxygen during the first 10 days of life. Rats were divided into six groups: (1) room-air normoxia (n = 11); (2) room-air, sham (n = 11); (3) hyperoxia-exposed / normal saline as placebo (n = 9); (4) hyperoxia-exposed/culture medium of MSCs(n = 10); (5) hyperoxia-exposed/ medium remaining after harvesting of MSCs (n = 8); and (6) hyperoxia-exposed/ MSCs (n = 17). Pathologic changes, number and diameter of alveoli, α-smooth muscle actin expression and localization of MSCs in the lungs were assessed. Number of alveoli increased and alveolar diameter decreased in the mesenchymal stem cell group so that there was no differences when compared with the normoxia group (respectively p = 0.126 and p = 0.715). Expression of α-smooth muscle actin decreased significantly in the mesenchymal stem cell group compared with the placebo group…
Turk Pediatri Arsivi-turkish Archives of Pediatrics, Apr 10, 2015
Bu çalışmada orta ya da ağır hipoksik iskemik ansefalopati tanısı alan yenidoğan bebeklere seçici... more Bu çalışmada orta ya da ağır hipoksik iskemik ansefalopati tanısı alan yenidoğan bebeklere seçici baş soğutma ya da tüm beden soğutma tedavisi uygulayarak hangi yöntemin daha üstün olduğunun araştırılması amaçlandı. Gereç ve Yöntemler: Çalışmaya 35. gebelik haftasından büyük, orta ya da ağır hipoksik iskemik ansefalopati tanısı alan yenidoğanlar alındı ve rastlantısal olarak seçici baş soğutma ya da tüm beden soğutma tedavisi uygulandı. Her iki yöntemle soğutma tedavisi uygulanan yenidoğanlar erken dönemde istenmeyen etkiler ve kısa dönem sonuçları açısından karşılaştırıldı. Çalışma için etik kurul izni alındı (06.01.2010/35). Bulgular: Hipoksik iskemik ansefalopati tanısı alan 53 bebek incelendi ve 17 bebeğe seçici baş soğutma, 12 bebeğe tüm beden soğutma uygulandı. Soğutma tedavisine bağlı olarak gelişebilecek istenmeyen etkiler açısından iki grup arasında fark bulunmadı. Erken dönem sonuçları incelendiğinde hastanede yatış süresi seçici baş soğutma grubunda 34 (7-65) gün iken, tüm beden soğutma grubunda 18 (7-57) gün idi ve aralarında anlamlı fark yoktu (p=0,097). Seçici baş soğutma grubundan dört, tüm beden soğutma grubundan iki hasta uzamış mekanik ventilasyon gereksinimi nedeniyle trakeostomi ile taburcu edildiler ve trakeostomi ile taburculuk açısından gruplar arasında fark yoktu (p=0,528). Seçici baş soğutma grubundan beş, tüm beden soğutma grubundan üç hasta ağızdan beslenememeleri nedeniyle gastrostomi tüpü ile taburcu edildiler ve gastrostomi tüpü ile taburculuk açısından gruplar arasında fark yoktu (p=0,586). Seçici baş soğutma tedavisi uygulanan bebeklerden biri, tüm beden soğutma uygulanan bebeklerden biri hastanede yaşamını kaybetti ve ölüm açısından gruplar arasında fark yoktu (p=0,665). Çıkarımlar: Seçici baş soğutma ve tüm beden soğutma yöntemleri arasında istenmeyen etkiler ve kısa dönem sonuçları açısından fark yoktur.
Hypoxic-ischemic brain injury that occurs in the perinatal period is one of the leading causes of... more Hypoxic-ischemic brain injury that occurs in the perinatal period is one of the leading causes of mental retardation, visual and auditory impairment, motor defects, epilepsy, cerebral palsy, and death in neonates. The severity of apoptosis that develops after ischemic hypoxia and reperfusion is an indication of brain injury. Thus, it may be possible to prevent or reduce injury with treatments that can be given before the reperfusion period following hypoxia and ischemia. Levetiracetam is a new-generation antiepileptic drug that has begun to be used in the treatment of epilepsy. The present study investigated the effects of levetiracetam on neuronal apoptosis with histopathological and biochemical tests in the early period and behavioral experiments in the late period. This study showed histopathologically that levetiracetam reduces the number of apoptotic neurons and has a neuroprotective effect in a neonatal rat model of hypoxic-ischemic brain injury in the early period. On the other hand, we demonstrated that levetiracetam dose dependently improves behavioral performance in the late period. Based on these results, we believe that one mechanism of levetiracetam&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s neuroprotective effects is due to increases in glutathione peroxidase and superoxide dismutase enzyme levels. To the best of our knowledge, this study is the first to show the neuroprotective effects of levetiracetam in a neonatal rat model of hypoxic-ischemic brain injury using histopathological, biochemical, and late-period behavioral experiments within the same experimental group.
BACKGROUND: The aim of this study was to determine if levetiracetam (LEV) is neuroprotective in n... more BACKGROUND: The aim of this study was to determine if levetiracetam (LEV) is neuroprotective in neonatal rats with hypoxic-ischemic brain injury (HIBI). METHODS: The study included 7-d-old male Wistar rats that were randomly divided into the LEV400, LEV800, control, and sham groups. All the rats, except those in the sham group, underwent ligation of the carotid artery and were then kept in a hypoxic chamber containing 8% oxygen for 2 h. At the end of the hypoxic period the rats in the control group were administered saline solution 0.5 mL, the rats in the LEV400 group were administered LEV 400 mg.kg-1 , and rats in the LEV800 group were administered LEV 800 mg.kg-1 via the intraperitoneal route. The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) method was used to evaluate neuronal apoptosis in the rats. The Morris Water Maze (MWM) test was performed at age 14 weeks in order to evaluate cognitive function. RESULTS: The number of apoptotic neurons in the right hemispheres was signifi cantly lower in the sham, LEV400, and LEV800 groups than in the control group (p < 0.001, p < 0.001, and p < 0.001, respectively). In addition, the number of apoptotic neurons in the right hemispheres was signifi cantly lower in the LEV800 group than in the LEV400 group (p = 0.001). Platform fi nding time (PFT) during MWM testing was signifi cantly shorter in the sham and LEV800 groups on d 4 than on d 1 (p = 0.001 and p = 0.006, respectively); however, PFT did not signifi cantly change between d 1 and d 4 in the control or LEV400 groups (p = 0.91 and p = 0.096, respectively). CONCLUSION: Based on the present fi ndings, LEV exhibited a dose-dependent neuroprotective effect in neonatal rats with HIBI (Ref. 27).
Turk Pediatri Arsivi-turkish Archives of Pediatrics, Jul 20, 2015
Ectopia cordis is a rare congenital malformation in which the heart is located partially or total... more Ectopia cordis is a rare congenital malformation in which the heart is located partially or totally outside the thoracic cavity. The estimated prevalence of ectopia cordis is 5.5-7.9 per million births and it comprises 0.1% of congenital heart diseases. Ectopia cordis is associated with other congenital heart diseases and various tissue and organ disorders. Common cardiac anomalies associated with ectopia cordis include ventricular septal defect, atrial septal defect, pulmonary stenosis, right ventricular diverticulum, double right ventricular outflow tract and tetralogy of Fallot. Extracardiac anomalies associated with ectopia cordis reported in the literature include omphalocele, gastrochisis, cleft lip and palate, scollosis and central nervous system malformations. Here we report a newborn with ectopia cordis who was diagnosed prenatally.
Özofagus atrezisi ve trakeoözofageal fistül nedeni ile kliniğimizde tedavi edilmiş hastalarda uyg... more Özofagus atrezisi ve trakeoözofageal fistül nedeni ile kliniğimizde tedavi edilmiş hastalarda uyguladığımız cerrahi yöntemler ve tedavi sonuçlarını irdelemektir.
Johanson Blizzard Sendromu (JBS) ender görülen; burun kanatlarının yokluğu veya az gelişmişliği, ... more Johanson Blizzard Sendromu (JBS) ender görülen; burun kanatlarının yokluğu veya az gelişmişliği, dişte yapısal bozukluklar, orta hat deri defektleri, büyüme ve zekâ geriliği, pankreas yetmezliği, anüsün doğuştan kapalı olması ve doğumsal sağırlıkla kendini gösteren bir durumdur. Otozomal resesif kalıtılır. Anüsün kapalı olması nedeniyle getirilen ve burun kanadında az gelişmişlik saptanan vakada JBS düşünülmüş ve ender rastlanması nedeniyle sunulmuştur.
Jeune sendromu asfiktik torasik distrofi olarak da isimlendirilen otozomal resesif geçişli çoklu ... more Jeune sendromu asfiktik torasik distrofi olarak da isimlendirilen otozomal resesif geçişli çoklu organ tutulumu olan bir iskelet displazisidir. Dar, çan şeklinde göğüs duvarı, farklı derecelerde kol ve bacak kısalığı bu sendromun en karakteristik özelliğidir. Jeune sendromlu çocukların çoğunda erken süt çocukluğu döneminde ağır solunum yetmezliği görülür. Burada doğumun hemen ardından solunum yetmezliği gelişen ve Jeune sendromu tanısı alan bir olgu nadir görülmesi nedeniyle sunulmuştur. Akraba evliliklerinin sık olduğu ülkemizde solunum sıkıntısı bulgularının yanında dar göğüs kafesi, kısa kol ve bacakların görüldüğü bebeklerde Jeune sendromu akla gelmelidir. AnAhtAr sözcüKler: Jeune sendromu, asfiktik torasik distrofi, yenidoğan Jeune syndrome, also known as asphyxiating thoracic dystrophy, is a rare autosomal recessive skeletal dysplasia with multiorgan involvement. Clinically, Jeune syndrome is characterised by a bell-shaped, narrow chest and variable limb shortness. Most of the children suffering from this syndrome present with severe respiratory failure during the early infancy period. Here we report a neonate with serious postnatal respiratory insufficiency who was diagnosed with Jeune syndrome. Since intermarriage is frequent in Turkey, when a newborn with respiratory failure has a bell-shaped, narrow chest and variable limb shortness, Jeune syndrome should be considered.
Accessory nostril is a very rare congenital anomaly with an unknown etiology also known as supern... more Accessory nostril is a very rare congenital anomaly with an unknown etiology also known as supernumerary nostril. A few accessory nostrils have been reported up to the present time, and extremely rare cases located on columella. A newborn infant with respiratory distress was referred to our hospital. The authors observed that accessory nasal nostril is not related to normal nasal cavity on the median line of columella. In this article, the authors reported accessory nostril case in newborn and review the literature.
Background: The aim of the study is to predict prognosis of newborns with congenital diaphragmati... more Background: The aim of the study is to predict prognosis of newborns with congenital diaphragmatic hernia (CDH) with the left ventricular diameter and estimated pulmonary artery pressure values. Methods: Patients in the newborn unit in 2012-2016 were screened retrospectively. Echocardiographic measurements of 35 patients with isolated left lateral CDH and 25 healthy newborn were compared in the first 24h of life. Results: The mean values of left ventricular end-diastolic (LVEDD) and end-systolic (LVESD) diameters, and ejection fraction were significantly lower in the patients. Moreover, tricuspid insufficiency (TR) and pulmonary insufficiency (PR) were significantly higher than controls (p<0.001). Six patients died within the next 40 days after birth. Mean values of LVEDD and LVESD were significantly lower in patients who died when compared to discharged (p<0.001, p=0.016). Also, mean values of TR and PR (p<0.001) and the frequency of pulmonary hypertension (PPH) (p=0.001) were significantly higher in these patients. In the ROC analysis of the patients who died LVEDD<11 mm [sensitivity 100% (95% CI:87.9-100) and specificity 100% (95% CI:54.1-100) and TR>3.5m/s [sensitivity 89.66% (95% CI:72.6-97.7) and specificity 100% (95% CI:54.1-100)] were demonstrated to be associated with poor prognosis. Conclusions: In this study, decrease in LV diameter and presence of pulmonary hypertension was shown to be associated with poor prognosis. Also, presence of PPH was found to be associated with mortality in patients with CDH. Thus, it is assumed that, in the follow-up prognosis may be predicted by a carefully echocardiographic evaluation of the LV diameters and pulmonary pressures.
Acta Orthopaedica et Traumatologica Turcica, Mar 5, 2020
The role of the platelet-to-lymphocyte ratio and neutrophil-to-lymphocyte ratio in the prediction... more The role of the platelet-to-lymphocyte ratio and neutrophil-to-lymphocyte ratio in the prediction of length and cost of hospital stay in patients with infected diabetic foot ulcers: A retrospective comparative study
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