Papers by Rosilaine Carrenho
Acta Botanica Brasilica, Dec 1, 2021
Arbuscular mycorrhizal fungi (AMF) and dark septate fungi (DSF) are symbionts that are associated... more Arbuscular mycorrhizal fungi (AMF) and dark septate fungi (DSF) are symbionts that are associated with the roots of plants, including epiphytic lycophytes and ferns. Paris-type mycorrhiza and glomoid structures are the most common forms of colonization in these plants. This work aimed to evaluate the occurrence of these symbionts in the roots of epiphytic lycophytes and ferns as well as the diversity of AMF spores recovered from substrate associated with the roots of eleven species. Roots of Asplenium gastonis, Campyloneurum aglaolepis, C. nitidum, Niphidium crassifolium, Pecluma pectinatiformis, Phlegmariurus mandiocanus, Pleopeltis hirsutissima, P. pleopeltifolia and Selaginella microphylla had hyphae and vesicles typical of AMF colonization, but not arbuscules. Campyloneurum nitidum, Pecluma pectinatiformis, Phlegmariurus mandiocanus, Pleopeltis pleopeltifolia and Selaginella microphylla had melanized hyphae and microsclerotia typical of DSF. All species colonized by DSF were also colonized by AMF. Seventeen spore morphotypes of AMF were identified, of which six were acaulosporoid and eleven glomoid. Glomus aff. formosanum and Acaulospora aff. lacunosa were the most abundant and frequent species. Epiphytic lycophytes and ferns host concurrently AMF and DSF but colonization is scanty in their roots. For the first time, acaulosporoid spores and intraradical vesicles are reported for this group of plants.
Pesquisa Agropecuaria Brasileira, Nov 1, 2017
The objective of this work was to evaluate the influence of aqueous extracts of nonmycorrhizal we... more The objective of this work was to evaluate the influence of aqueous extracts of nonmycorrhizal weedy species on dry matter accumulation and assimilate partitioning of maize plants, with or without Cetraspora pellucida inoculation. The experiment was carried out in pots, in a completely randomized design, in a 5x2 factorial arrangement consisting of four plant extracts (purple nutsedge, guinea-hen weed, slender amaranth, and knotweed), a control irrigated with water, and two conditions (mycorrhizal and nonmycorrhizal maize plants), with four replicates of each treatment. Plants were irrigated with aqueous extracts diluted at 15%. Root colonization (RC), shoot dry matter (SDM), root dry matter (RDM), and RDM/ SDM were evaluated. Root colonization was not influenced by the extracts, and ranged from 41.5 to 65.2%. Shoot dry matter of mycorrhizal (AM) and nonmycorrhizal (NM) plants was not influenced by the extracts; however RDM showed varying responses. Mycorrhization favored the production of RDM, and increased plant sensitiveness to the extracts. Guinea-hen weed extract increased RDM of AM plants, while the other extracts inhibited it. In NM plants, the production of RDM was benefited by slender amaranth extract, to the detriment of SDM. Mycorrhizal and nonmycorrhizal maize plants respond differently to aqueous extracts of nonmycorrhizal weeds.
Este trabalho teve como objetivo avaliar a relação entre as características morfológicas de raíze... more Este trabalho teve como objetivo avaliar a relação entre as características morfológicas de raízes de gramíneas e os fungos micorrízicos arbusculares (FMA). Amostras de solo e de raízes foram coletadas nas camadas superficiais para determinação do volume, comprimento total e específico, diâmetro e massa seca das raízes, colonização radical por FMA e número de esporos. Os caracteres morfológicos das raízes se mostraram pouco relacionados com a colonização micorrízica. Observou-se correlação negativa entre colonização radical e comprimento total de raízes, e correlação entre número de esporos e diâmetro de raízes.
In nature, there are some intraor interspecific ecological relations with different dependence le... more In nature, there are some intraor interspecific ecological relations with different dependence levels. Plant roots are good examples of this, because they develop ecological relations with many soil microorganisms, mainly with the fungi, forming mycorrhizae. The interest in studying this association has risen up due to increasing necessity of alternative handling methods that promote improvement in the plant growth and productivity. Arbuscular mycorrhiza have been known and explored for a long time due to the ecological significance and effects in the growth and nutrition of plants, playing an important role for the nutrient uptake system. The mycorrhizal establishment in eucalyptus has been known for many years, and the benefits of this symbiosis have been commercially explored. The most recent outcomes regarding the occurrence, importance and use of arbuscular mycorrhiza in Eucalyptus spp. are presented in this paper. Firstly, these will be grouped in various research topics (stat...
Pesquisa Agropecuária Brasileira, Nov 1, 2017
The objective of this work was to evaluate the influence of aqueous extracts of nonmycorrhizal we... more The objective of this work was to evaluate the influence of aqueous extracts of nonmycorrhizal weedy species on dry matter accumulation and assimilate partitioning of maize plants, with or without Cetraspora pellucida inoculation. The experiment was carried out in pots, in a completely randomized design, in a 5x2 factorial arrangement consisting of four plant extracts (purple nutsedge, guinea-hen weed, slender amaranth, and knotweed), a control irrigated with water, and two conditions (mycorrhizal and nonmycorrhizal maize plants), with four replicates of each treatment. Plants were irrigated with aqueous extracts diluted at 15%. Root colonization (RC), shoot dry matter (SDM), root dry matter (RDM), and RDM/ SDM were evaluated. Root colonization was not influenced by the extracts, and ranged from 41.5 to 65.2%. Shoot dry matter of mycorrhizal (AM) and nonmycorrhizal (NM) plants was not influenced by the extracts; however RDM showed varying responses. Mycorrhization favored the production of RDM, and increased plant sensitiveness to the extracts. Guinea-hen weed extract increased RDM of AM plants, while the other extracts inhibited it. In NM plants, the production of RDM was benefited by slender amaranth extract, to the detriment of SDM. Mycorrhizal and nonmycorrhizal maize plants respond differently to aqueous extracts of nonmycorrhizal weeds.
Acta Botanica Brasilica, 2021
Arbuscular mycorrhizal fungi (AMF) and dark septate fungi (DSF) are symbionts that are associated... more Arbuscular mycorrhizal fungi (AMF) and dark septate fungi (DSF) are symbionts that are associated with the roots of plants, including epiphytic lycophytes and ferns. Paris-type mycorrhiza and glomoid structures are the most common forms of colonization in these plants. This work aimed to evaluate the occurrence of these symbionts in the roots of epiphytic lycophytes and ferns as well as the diversity of AMF spores recovered from substrate associated with the roots of eleven species. Roots of Asplenium gastonis, Campyloneurum aglaolepis, C. nitidum, Niphidium crassifolium, Pecluma pectinatiformis, Phlegmariurus mandiocanus, Pleopeltis hirsutissima, P. pleopeltifolia and Selaginella microphylla had hyphae and vesicles typical of AMF colonization, but not arbuscules. Campyloneurum nitidum, Pecluma pectinatiformis, Phlegmariurus mandiocanus, Pleopeltis pleopeltifolia and Selaginella microphylla had melanized hyphae and microsclerotia typical of DSF. All species colonized by DSF were also colonized by AMF. Seventeen spore morphotypes of AMF were identified, of which six were acaulosporoid and eleven glomoid. Glomus aff. formosanum and Acaulospora aff. lacunosa were the most abundant and frequent species. Epiphytic lycophytes and ferns host concurrently AMF and DSF but colonization is scanty in their roots. For the first time, acaulosporoid spores and intraradical vesicles are reported for this group of plants.
Pesquisa Agropecuária Tropical, 2016
The adoption of biological resources in agriculture may allow less dependence and better use of f... more The adoption of biological resources in agriculture may allow less dependence and better use of finite resources. This study aimed at evaluating the effects of inoculation with arbuscular mycorrhizal fungi native to the Brazilian Savannah associated with the application of mycorrhizal stimulant (7-hydroxy, 4'-methoxy-isoflavone), in the early growth of common bean and soybean. The experiment was carried out in a greenhouse, in a completely randomized design, with a 7 x 2 factorial arrangement, consisting of five arbuscular mycorrhizal fungi species, joint inoculation (junction of all species in equal proportions) and native fungi (without inoculation), in the presence and absence of stimulant. The following traits were evaluated: shoot dry matter, root dry matter, mycorrhizal colonization, nodules dry matter and accumulation of calcium, zinc and phosphorus in the shoot dry matter. The increase provided by the arbuscular mycorrhizal fungi and the use of stimulant reached over 200...
Scientia Horticulturae
The structure of arbuscular mycorrhizal fungi (AMF) communities in areas under strawberry cultiva... more The structure of arbuscular mycorrhizal fungi (AMF) communities in areas under strawberry cultivation in the Brazilian subtropics has been poorly studied. Thus, we characterized cultivated and native forest soils, of reference-sites in strawberry cultivation in southern Brazil, regarding the AMF community and investigate if there is variability in the diversity in the mycorrhizal communities of these soils. For this, we evaluated eight soils, in a completely randomized design experiment. We observed that the species Claroideoglomus aff. luteum, Claroideoglomus claroideum, C. etunicatum, Funneliformis mosseae and Glomus sp2 were the most frequent. Native forest soils had more fungal species than cultivated soils. Soils little anthropized under strawberry cultivation resembled the natural soils regarding the AMF heterogeneity. We conclude that there are AMF diversity in soils of reference-sites in strawberry cultivation, and that C. claroideum and C. etunicatum are generalist, independent of the ecosystem. This study contributes significantly to our knowledge about the community composition of AMF in soils of reference-sites in the strawberry cultivation in the Brazilian subtropics. Thus, it becomes possible the development of inoculants for this horticultural crop, with the role of making their cultivation more sustainable.
Climate Change and Soil Interactions, 2020
Abstract The environmental variables associated with the global warming influence on every life l... more Abstract The environmental variables associated with the global warming influence on every life level. Growth and developmental alteration of plants and main responsible components for the atmosphere accumulated carbon sequestration interfere not only on quantity and quality of those as a food source for the first-level animal consumers but also on the settling of trophic interactions from next levels. The carbon flux through both construction and deconstruction of organic matter depends on the primary and secondary metabolism of plants, as well as present organism’s actions in the soil on the accumulated superficial matter. The recalcitrant materials’ production is directly associated with the plant’s life condition. Stresses caused by the increase in temperature commonly stimulate the formation of protective secondary compounds on plants. Those matters can stimulate the organic carbon persistence both by the molecule chemical structure and by hindering the development and action of decomposers on dead matter.
Bio-Geotechnologies for Mine Site Rehabilitation, 2018
Abstract Rehabilitation of mined areas depends directly on the reestablishment of vegetation cove... more Abstract Rehabilitation of mined areas depends directly on the reestablishment of vegetation cover to areas that were denuded or degraded. However, the presence of unfavorable environmental conditions, such as low water availability and high concentrations of toxic metals, is limiting factors for the success of rehabilitation programs. This chapter highlights the role of mycorrhizal fungi in abandoned mine land remediation and discusses evidence that mycorrhizal plants are more efficient than nonmycorrhizal plants at phytoremediation of contaminated soils. By stimulating lateral root formation, increasing soil exploration and the supply of nutrients to the plant, mycorrhizal fungi can promote not only greater plant growth but also increase phytoremediation capacity. The importance of prospecting indigenous species of mycorrhizal fungi from areas to be remediated is also discussed.
Os Fungos Micorrizicos Arbusculares (FMA) desempenham papel fundamental para a manutencao da prod... more Os Fungos Micorrizicos Arbusculares (FMA) desempenham papel fundamental para a manutencao da produtividade e saude dos vegetais. Portanto, o manejo adequado da associacao micorrizica pode reduzir a utilizacao de fertilizantes e pesticidas quimicos, chave para a producao sustentavel. Com o objetivo de verificar o impacto de duas formas de manejo, convencional e orgânica, sobre esta associacao, realizou-se um levantamento em pomares e viveiros de citros localizados em duas regioes pertencentes ao municipio de Montenegro/RS. Foram realizadas coletas de raizes e solo adjacente as raizes, nos meses de marco e agosto de 2002, para avaliar as comunidades de FMA, a colonizacao radicular no citros e as caracteristicas quimicas do solo. Como resultado, observou-se que os sistemas produtivos, orgânico e convencional, apresentaram comunidades de FMA semelhantes, e as estruturas dos FMA mais afetadas pelos manejos foram as vesiculas e os arbusculos. Palavras-chave: citros, comunidades de FMA, co...
1Doutoranda do Programa de Pós-Graduação em Biologia Comparada, Universidade Estadual de Maringá,... more 1Doutoranda do Programa de Pós-Graduação em Biologia Comparada, Universidade Estadual de Maringá, Maringá, PR. Bolsista CAPES. [email protected] 2Orientadora, Doutora, Docente, Departamento de Biologia, Universidade Estadual de Maringá, Maringá, PR. [email protected] 3 Co-orientador, Doutor, Docente, Departamento de Engenharia Agrícola, Universidade Estadual de Maringá, Campus do Arenito, Cidade Gaúcha, PR. [email protected]
Both the quality and the quantity of organic matter placed in the soil are variables influenced b... more Both the quality and the quantity of organic matter placed in the soil are variables influenced by the alterations in the climate conditions of the environment produced by global warming. The CO2 increase in the atmosphere stimulates the production of biomass on plants that firstly would be supporting a bigger quantity of organisms associated with the food chain. However, significant climate alterations can modify the proportion of the present formatives in plant tissues affecting both their palatability and resistance in the decomposition. Plants are the organisms that mostly contribute to the organic matter input in the soil, and most parts of the decomposers’ functional groups act on the dead matter from these organisms. Increases in temperature, decreases in humidity, and the CO2 raising concentration lead to changes in both diversity and metabolism from the decomposers’ microorganisms, which can be harmful to different ecosystem levels, depending on the type and speed of reacti...
Applied Soil Ecology, 2021
Abstract Control of agricultural pathogens can be achieved by biological control agents, but info... more Abstract Control of agricultural pathogens can be achieved by biological control agents, but information about their impacts on the native microbiota is scarce. In this work, the commercial biocontrol agent Trichoderma harzianum ESALQ-1306 and the experimental strain Trichoderma asperellum BRM-29104 were selected to determine the effects of their application on the edaphic microbial community and on their functional traits in common bean cultivation (Phaseolus vulgaris L.) infected with Sclerotinia sclerotiorum (Lib.) de Bary in the Brazilian savannah. Soil samples were collected before planting and after 90 days of cultivation. The bean yield and disease severity were estimated. Spores of arbuscular mycorrhizal fungi (AMF) were quantified and identified, and the rate of root colonisation was measured. The total colony-forming units (CFU), species richness and alpha diversity, production of hydrolytic enzymes, microbial carbon biomass, microbial soil respiration, metabolic diversity and glomalin mass were quantified. After 90 days of cultivation, the microbial richness, diversity and abundance increased significantly, stimulated by bean plant development. The biocontrol strains did not significantly change the abundance of CFU with enzymatic activity, as well as microbial soil respiration, the microbial carbon biomass, metabolic diversity, the glomalin mass and the root colonisation rates; however, they changed the composition of cultivable microbial communities. S. sclerotiorum was controlled, and bean productivity was maintained. Therefore, the cultivation time is the variable with the major influence over the structure and functionality of cultivable microbial communities and AMF. We identified microbial species not previously reported in this biome and determined that T. asperellum BRM-29104 has the potential to be used as a new eco-friendly commercial product against white mold.
Climate Change and Soil Interactions, 2020
Abstract The emission of excessive volumes of gases from the greenhouse effect not only increases... more Abstract The emission of excessive volumes of gases from the greenhouse effect not only increases the air, water, and soil’s temperatures but also affects the ozone distribution on both troposphere and stratosphere interfering on the living beings in different ways. Higher ultraviolet radiation incidence, the increase in temperature, the reduction in water availability, salt stress, and high CO2 levels are some factors that might affect the development of both plants and root-associated mutualistic fungi. Losses on the vigor of one or both symbionts conceivably imply changes in expected benefits from the mycorrhizal association. Having most part of their bodies inhabiting the aerial environment, the plants are the organisms mostly exposed to the stresses caused by global warming. Mycorrhizal fungi, which inhabit natural extraradical habitats as the soil, are likely to be less affected than the ones which settle on the aerial environment like the orchid epiphytes or saxicolous associated ones.
Rhizosphere, 2020
Abstract This study aimed to evaluate the influence of arbuscular mycorrhizal fungi on the growth... more Abstract This study aimed to evaluate the influence of arbuscular mycorrhizal fungi on the growth and production of capitula and essential oil of chamomile plants, under different growth conditions. The symbiosis between fungi of the phylum Glomeromycota and plant roots may cause changes in secondary metabolism, including the synthesis of essential oils. Increasing biomass production associated with gains in oil content promoted by mycorrhization, makes soil biota management sustainable. Two assays were carried out in a greenhouse, in plastic pots (3 L), for 150 days after emergence of the seedlings. In the first assay, plants were inoculated with soil cultivated with organic chamomile, in three levels (0, 150 and 300 g), with pH adjusted at 6.5, for evaluating the influence of the quantity of inoculum on the growth and production of plants. In the second assay (factorial design with repetition at the central point), three levels of soil pH (6.5, 7.0, 7.5) and three levels of auxin (0; 10; 20 mg L−1) were tested to identify the influence of pH and auxin on the production of chamomile capitula and essential oil. The arbuscular mycorrhizal fungi present as spores in the soil-inoculum were composed of 22 species. In the first assay, mycorrhization favored the growth and production of capitula and such benefits tended to be higher with the increase in concentration of inoculum. In the second assay, the production of capitula and essential oil was promoted by mycorrhization, with the highest content at neutral pH (0.7 mL oil in 30 g of capitula), in synergy with the highest amount of inoculum. It was concluded that the presence of arbuscular mycorrhizal fungi in the soil is the most influential variable in the production of capitula and essential oil of chamomile, but subtle differences on soil pH influenced these parameters. The levels of auxin did not influence significantly plant growth and arbuscular mycorrhizal fungi.
Climate Change and Soil Interactions, 2020
Abstract Both the quality and the quantity of organic matter placed in the soil are variables inf... more Abstract Both the quality and the quantity of organic matter placed in the soil are variables influenced by the alterations in the climate conditions of the environment produced by global warming. The CO2 increase in the atmosphere stimulates the production of biomass on plants that firstly would be supporting a bigger quantity of organisms associated with the food chain. However, significant climate alterations can modify the proportion of the present formatives in plant tissues affecting both their palatability and resistance in the decomposition. Plants are the organisms that mostly contribute to the organic matter input in the soil, and most parts of the decomposers’ functional groups act on the dead matter from these organisms. Increases in temperature, decreases in humidity, and the CO2 raising concentration lead to changes in both diversity and metabolism from the decomposers’ microorganisms, which can be harmful to different ecosystem levels, depending on the type and speed of reactions and on both the immediate and long-term result from the decomposition.
Pesquisa Agropecuária Brasileira, 2004
Os fungos micorrízicos arbusculares (FMA) apresentam um grande potencial biotecnológico, mas, par... more Os fungos micorrízicos arbusculares (FMA) apresentam um grande potencial biotecnológico, mas, para que seu emprego seja bem-sucedido, é necessário conhecer como esses organismos respondem às práticas agrícolas. O objetivo deste trabalho foi avaliar o efeito de duas formas de manejo, convencional e orgânica, em pomares e viveiros de citros, nas comunidades de FMA, comparadas com solo de mata nativa. Um total de 36 amostras de solo foram coletadas, e suas características químicas e a ocorrência de espécies de FMA foram avaliadas. Os sistemas de manejo não alteraram as comunidades de FMA, apesar das modificações químicas no solo causadas pelas aplicações de fertilizantes orgânicos, que elevaram os valores de pH, matéria orgânica, Ca e magnésio. No entanto, as comunidades foram afetadas pelo tempo de implantação e pelas regiões onde se localizam os pomares e viveiros. Estas diferenças se devem provavelmente à estabilidade dos pomares mais antigos e às características evolutivas de cada ...
Uploads
Papers by Rosilaine Carrenho