Papers by Ziba Hedayatnasab
International Journal of Nanomedicine
Introduction: Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) with multiple functionalities are intriguing ... more Introduction: Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) with multiple functionalities are intriguing candidates for various biomedical applications. Materials and Methods: This study introduced a simple and green synthesis of Fe 3 O 4 NPs using a low-cost stabilizer of plant waste extract rich in polyphenols content with a well-known antioxidant property as well as anticancer ability to eliminate colon cancer cells. Herein, Fe 3 O 4 NPs were fabricated via a facile co-precipitation method using the crude extract of Garcinia mangostana fruit peel as a green stabilizer at different weight percentages (1, 2, 5, and 10 wt.%). The samples were analyzed for magnetic hyperthermia and then in vitro cytotoxicity assay was performed. Results: The XRD planes of the samples were corresponding to the standard magnetite Fe 3 O 4 with high crystallinity. From TEM analysis, the green synthesized NPs were spherical with an average size of 13.42±1.58 nm and displayed diffraction rings of the Fe 3 O 4 phase, which was in good agreement with the obtained XRD results. FESEM images showed that the extract covered the surface of the Fe 3 O 4 NPs well. The magnetization values for the magnetite samples were ranging from 49.80 emu/g to 69.42 emu/g. FTIR analysis verified the functional groups of the extract compounds and their interactions with the NPs. Based on DLS results, the hydrodynamic sizes of the Fe 3 O 4 nanofluids were below 177 nm. Furthermore, the nanofluids indicated the zeta potential values up to −34.92±1.26 mV and remained stable during four weeks of storage, showing that the extract favorably improved the colloidal stability of the Fe 3 O 4 NPs. In the hyperthermia experiment, the magnetic nanofluids showed the acceptable specific absorption rate (SAR) values and thermosensitive performances under exposure of various alternating magnetic fields. From results of in vitro cytotoxicity assay, the killing effects of the synthesized samples against HCT116 colon cancer cells were mostly higher compared to those against CCD112 colon normal cells. Remarkably, the Fe 3 O 4 NPs containing 10 wt.% of the extract showed a lower IC 50 value (99.80 µg/mL) in HCT116 colon cancer cell line than in CCD112 colon normal cell line (140.80 µg/mL). Discussion: This research, therefore, introduced a new stabilizer of Garcinia mangostana fruit peel extract for the biosynthesis of Fe 3 O 4 NPs with desirable physiochemical properties for potential magnetic hyperthermia and colon cancer treatment.
Chemical Papers
Superparamagnetic iron oxide nanoparticles (SPIONs) are considered as promising magnetic nanoheat... more Superparamagnetic iron oxide nanoparticles (SPIONs) are considered as promising magnetic nanoheating agents for diagnostic as well as therapeutic applications due to their biocompatibility and tunability of magnetic properties. These nanoheating agents are commonly synthesized by coprecipitation of two iron precursors, though applying less amount of these chemicals may minimize the toxicity risks for biomedical purposes. The aim of this study is to address this issue by considering the high oxidation vulnerability of ferrous ions to ferric ions to synthesize SPIONs via a single-iron precursor under four varied oxidative conditions. The obtained results implied that the properties of SPIONs can be modified by the variation in the oxidizing conditions. Thereby, the optimal sample was produced as the oxygen/nitrogen flow ratio adjusted to 50% in the synthesis environment. The induction heating efficiency of this optimal sample was investigated under the exposure of varied alternating magnetic field (AMF), which resulted in a remarkable specific absorption rate (SAR) of 168.96 W g−1 while maintaining the temperature medium within the secure hyperthermia range. The cytotoxic effect of the optimal SPIONs sample against human liver carcinoma (HepG2) cells was determined using MTT assay, leading to a considerable decrement in the cell viability. The incorporation of SPIONs under the AMF exposure is, therefore, considered as an effectual mechanism to the tumor intervention.
Chemical Papers , 2020
Superparamagnetic iron oxide nanoparticles (SPIONs) are considered as promising magnetic nanoheat... more Superparamagnetic iron oxide nanoparticles (SPIONs) are considered as promising magnetic nanoheating agents for diagnostic as well as therapeutic applications due to their biocompatibility and tunability of magnetic properties. These nanoheating agents are commonly synthesized by coprecipitation of two iron precursors, though applying less amount of these chemicals may minimize the toxicity risks for biomedical purposes. The aim of this study is to address this issue by considering the high oxidation vulnerability of ferrous ions to ferric ions to synthesize SPIONs via a single-iron precursor under four varied oxidative conditions. The obtained results implied that the properties of SPIONs can be modified by the variation in the oxidizing conditions. Thereby, the optimal sample was produced as the oxygen/nitrogen flow ratio adjusted to 50% in the synthesis environment. The induction heating efficiency of this optimal sample was investigated under the exposure of varied alternating magnetic field (AMF), which resulted in a remarkable specific absorption rate (SAR) of 168.96 W g−1 while maintaining the temperature medium within the secure hyperthermia range. The cytotoxic effect of the optimal SPIONs sample against human liver carcinoma (HepG2) cells was determined using MTT assay, leading to a considerable decrement in the cell viability. The incorporation of SPIONs under the AMF exposure is, therefore, considered as an effectual mechanism to the tumor intervention.
Materials Research Bulletin, 2020
Co-precipitation of ferrous and ferric ions is the most common approach for synthesis of superpar... more Co-precipitation of ferrous and ferric ions is the most common approach for synthesis of superparamagnetic iron oxide nanoparticles (SPIONs). However, reducing the amount of applied chemicals could lessen toxicity risks for biomedical applications. This study aimed to apply the high oxidation vulnerability of ferrous ions for synthesis of SPIONs via a single-precursor route in varied oxidative conditions. The obtained results indicated that physicochemical and magnetic properties of SPIONs were purposefully tunable through alteration of oxidative conditions and the optimized SPIONs were produced when the O2:N2 flow ratio adjusted to 5:5 in synthesis environment. The induction heating efficiency of the optimal SPIONs with different concentrations was evaluated under exposure of various alternating magnetic field (AMF). The cytotoxic activity of these SPIONs on human liver carcinoma (HepG2) cells under hyperthermia condition indicated a noticeable reduction in cell viability to 49 ± 0.3 %, rendering these magnetic nano-heating agents as promising candidates for cancer therapy purposes.
European Polymer Journal, 2020
Magnetic hyperthermia therapy of cancer is a promising alternative for the current chemotherapy a... more Magnetic hyperthermia therapy of cancer is a promising alternative for the current chemotherapy and radiation options, due to its targeting capability and lower systemic toxicity. However, agglomeration vulnerability of the nano-heating agents in aqueous solutions, low control over the heat generation and dissipation at the target region, and eschewed clearance by the renal and reticuloendothelial systems remain as the main challenges for clinical translation of this approach. Here we report superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by using a single precursor, stabilized with a controlled micellar conformation, and coated with a thin polycaprolactone biopolymer shell to achieve enhanced cytocompatibility and thermosensitivity under external alternating magnetic field (AMF) of varied intensities. The in vitro investigations indicated a negligible influence of the polymer-coated SPIONs on cell viability of human liver cancer cells (HepG2) at a maximum concentration of 100 μg∙mL-1. However, the cell viability was reduced significantly to 40.1 ± 0.9% within the secure hyperthermia temperature range achieved by the heating effect of polymer-coated SPIONs in presence of AMF, depicting the on/off cytotoxicity phenomenon of polymer-coated SPIONs under AMF exposure. Therefore, the developed nanostructure presents a step-change as potential targeted nano-heating agents for new therapeutic options.
The present study reports a new idea for synthesis of highly stable superparamagnetic iron oxide ... more The present study reports a new idea for synthesis of highly stable superparamagnetic iron oxide nanoparticles (IONPs) in a mild alkaline anaerobic (N2) environment and compare their crystalline structure, zeta potential, aggregation vulnerability, and magnetic characteristics with those of IONPs produced through common methods, using either strong alkaline reagent (NaOH) and/or aerobic environment. The IONPs were precipitated at 80 °C temperature from Ferric and ferrous precursors using ammonium hydroxide (NH4OH) or sodium hydroxide as mild and strong alkaline reagents, respectively. The obtained results indicated the crucial impact of the alkaline reagents on the zeta potential and particle morphology, while the synthesis atmosphere mainly governed the crystalline structure and magnetic properties. Although the IONPs precipitated with NaOH under anaerobic environment exhibited higher zeta potential and thus slightly less aggregation susceptibility, those synthesized using NH4OH in anaerobic environment provided smaller particle size with enhanced crystallinity and magnetic properties. Therefore, the anaerobic environment with NH4OH as the precipitating agent was suggested as the optimal synthesis condition, resulting in particles that possessed superior crystallinity with crystallite size of 14 nm and high stable superparamagnetic properties, with saturation magnetization of 59.62 emu·g–1 and nearly zero coercivity.
Purpose: Although magnetite nanoparticles (MNPs) are promising agents for hyperthermia therapy, i... more Purpose: Although magnetite nanoparticles (MNPs) are promising agents for hyperthermia therapy, insufficient drug encapsulation efficacies inhibit their application as nanocarriers in the targeted drug
delivery systems. In this study, porous magnetite nanoparticles (PMNPs) were synthesized and coated with a thermosensitive polymeric shell to obtain a synergistic effect of hyperthermia and chemotherapy.
Materials and methods: PMNPs were produced using cetyltrimethyl ammonium bromide template and then coated by a polyethylene glycol layer with molecular weight of 1500Da (PEG1500) and phase transition temperature of 48 ± 2 C to endow a thermosensitive behavior. The profile of drug release from the nanostructure was studied at various hyperthermia conditions generated by waterbath, magnetic resonance-guided focused ultrasound (MRgFUS), and alternating magnetic field (AMF). The in vitro cytotoxicity and hyperthermia efficacy of the doxorubicin-loaded nanoparticles (DOXPEG1500-PMNPs) were assessed using human lung adenocarcinoma (A549) cells.
Results: Heat treatment of DOX-PEG1500-PMNPs containing 235 ± 26mgg1 DOX at 48 C by waterbath, MRgFUS, and AMF, respectively led to 71 ± 4%, 48 ± 3%, and 74 ±5% drug release. Hyperthermia treatment of the A549 cells using DOX-PEG1500-PMNPs led to 77% decrease in the cell viability due to the synergistic effects of magnetic hyperthermia and chemotherapy.
Conclusion: The large pores generated in the PMNPs structure could provide a sufficient space for encapsulation of the chemotherapeutics as well as fast drug encapsulation and release kinetics, which together with thermosensitive characteristics of the PEG1500 shell, make DOX-PEG1500-PMNPs promising adjuvants to the magnetic hyperthermia modality.
The application of superparamagnetic nanoparticles as heating agents in hyperthermia therapy has ... more The application of superparamagnetic nanoparticles as heating agents in hyperthermia therapy has made a therapeutic breakthrough in cancer treatment. The high efficiency of this magnetic hyperthermia therapy has derived from a great capability of superparamagnetic nanoparticles to generate focused heat in inaccessible tumors being effectively inactivated. The main challenges of this therapy are the improvement of the induction heating power of superparamagnetic nanoparticles and the control of the hyperthermia temperature in a secure range of 42 °C to 47 °C, at targeted area. The variation of these hyperthermia properties is principally dependent on the magnetic nanoparticles as well as the magnetic field leading to enhance the efficiency of magnetic hyperthermia therapy at targeted area and also avoid undue heating to healthy cells. The present study evaluates the magnetic hyperthermia therapy through the determination of superparamagnetic nanoparticles properties and magnetic field' parameters.
Hyperthermia cancer atherapy designed by magnetic particles as heating nano-mediators has been gr... more Hyperthermia cancer atherapy designed by magnetic particles as heating nano-mediators has been greatly applied for in vitro purposes to make reliable and certain conditions for in vivo trials. This intracellular treatment has found higher efficiency as compared to conventional ones due to generating heat locally through superparamagnetic nanoparticles for inaccessible tumors with minimal damage to the healthy cells nearby. The main challenges of this novel cancer therapy are the enhancement of heating power of such nanoparticles and the control of the local tumoral temperature. Those hyperthermia factors basically derived from magnetic nanoparticles as well as magnetic field. Thereby, the efficiency of magnetic hyperthermia is principally dependent on the proper determination of their features. This study tried to provide a comprehensive evaluation on the magnetic hyperthermia therapy through the determination of magnetic nanoparticles such as surface chemistry, intrinsic and extrinsic magnetic properties. In addition, the features of the magnetic field that substantially play on induction heating power and hyperthermia temperature are reviewed.
In this article, the polypropylene (PP) nanocomposites containing nanoclay particles and basalt f... more In this article, the polypropylene (PP) nanocomposites containing nanoclay particles and basalt fiber (BF) are prepared in the presence of maleic anhydride grafted PP (PP-g-MA) as compatibilizing agent. The Charpy impact behavior of PP/PP-g-MA/clay nanocomposites and PP/PP-g-MA/Clay/BF nanocomposites at room temperature (25°C), cryogenic temperature (–196°C), and high temperature (120°C) is presented. The BFs prevented the PP matrix from plastic deformation, but the impact strength improvement of nanocomposites is obtained when nanoclay in the PP matrix resists the propagation of cracks. The fracture surfaces are examined with scanning electron microscopy (SEM).
In this paper, a comparative study on the tensile properties of clay reinforced polypropylene (PP... more In this paper, a comparative study on the tensile properties of clay reinforced polypropylene (PP) nanocomposites (PPCN) and chopped basalt fiber reinforced PP–clay nanocomposites (PPCN-B) is presented. PP matrix are filled with 1, 3 and 5 wt.% of nanoclays. The ultimate tensile strength, yield strength, Young’s modulus and toughness are measured at various temperature conditions. The thermal conditions are included the room temperature (RT), low temperature (LT) and high temperature (HT). The basal spacing of clay in the composites is measured by X-ray diffraction (XRD). Nanoscale morphology of the samples is observed by transmission electron microscopy (TEM). Addition of nanoclay improves the yield strength and Young’s modulus of PPCN and PPCN-B; however, it reduces the ultimate tensile strength. Furthermore, the addition of chopped basalt fibers to PPCN improves the Young’s modulus of the composites. The Young’s modulus and the yield strength of both PPCN and PPCN-B are significantly high at LT (−196 °C), descend at RT (25 °C) and then low at HT (120 °C).
This paper focuses on the influence of temperature conditions and the clay contents on enhancemen... more This paper focuses on the influence of temperature conditions and the clay contents on enhancement of mechanical characterization of polypropylene (PP) nanocomposites. The nanocomposites were prepared using the melt mixing technique in a co-rotating intermeshing twin screw extruder followed by injection moulding. Nanocomposites properties such as impact strength and ultimate tensile strength, yield strength, failure strain, Young’s modulus and toughness are calculated. The addition of clay to PP matrix was showed remarkable enhancement in mechanical properties at the temperature of 25 oC and 120 °C. Nearly 36 % and 160 % increase in the Young’s modulus and about 45 % and 62 % increase in the impact strength were observed at both room temperature (RT) and high temperature (HT), respectively. But, the tensile strength was not affected much. The basal spacing of clay in the composites was measured by X-ray diffraction (XRD). Scanning electron microscopy (SEM) was used to assess the surface morphology of the fractured surfaces and dispersion of the nanoclay.
Polymer–clay nanocomposites are a new class of materials which improve the properties at low leve... more Polymer–clay nanocomposites are a new class of materials which improve the properties at low levels of addition of nanoclay as compared with conventional filler composites. In this article, a comparative study on the mechanical behavior of nanoclay filled polypropylene composites at both room and cryogenic temperatures is presented. Nanoclays of 1–5% by weight are added to polypropylene matrix using maleic anhydride grafted polypropylene as compatibilizer. Polypropylene/clay nanocomposites are prepared by melt intercalation in a twin-screw extruder followed by injection molding. Composite properties such as tensile and impact at both room (25°C) and cryogenic (−196°C) temperatures are calculated. The addition of clay to polypropylene matrix showed a remarkable enhancement in the mechanical properties at temperatures of 25°C and −196°C. Nearly 36.4% and 17.6% increase in the Young’s modulus and about 45% and 62.5% increase in the impact strength are observed at both room and cryogenic temperatures, respectively. Scanning electron microscopy is used to assess the surface morphology of the fractured surfaces and dispersion of the nanoclay. The morphology of polypropylene/clay nanocomposites is also characterized by X-ray diffraction analysis and transmission electron microscopy.
In this paper, the polypropylene (PP)nanocomposites containing 1, 3 and 5 wt % of nanoclay partic... more In this paper, the polypropylene (PP)nanocomposites containing 1, 3 and 5 wt % of nanoclay particles are prepared via direct melt mixing in the presence of maleic anhydride grafted PP (PP-g-MA) as compatibilizing agent. PP-g-MA is known to facilitate the dispersion of clay particles in a nonpolar PP matrix and to increase the adhesion between PP and the clay particles.The effect of different nanoclay contents on the PP composites are investigated for tensile characterization at both room temperature(RT) and cryogenic temperature (CT).The results showed that the cryogenic tensile strength, Young’s modulus, percentage of displacement at break and the energy absorptionat cryogenic temperature are all enhanced ascompared to the neat PP by the addition of clay at appropriate contents
In this paper, Polypropylene (PP) nanocomposites are prepared by melt mixing in a twin-screw extr... more In this paper, Polypropylene (PP) nanocomposites are prepared by melt mixing in a twin-screw extruder by injection molding. The role of compatibilizing agent is performed by maleic anhydride grafted polypropylene (PP-g-MA) between nanoclay and PP. The effect of nanoclay particles (1, 3, 5 wt %) on the PP composites is investigated for tensile test at high temperature for the first time. Mechanical behaviors of PP/clay nanocomposites at both room temperature (RT) and high temperature (HT) are investigated in terms of tensile properties. Addition of nanoclay showed a significant enhancement in stiffness of PP/clay nanocomposites. Nearly 36% and 157% increase in the tensile modulus at both RT and HT are observed, respectively. But, the increase in tensile strength is almost negligible.
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Papers by Ziba Hedayatnasab
delivery systems. In this study, porous magnetite nanoparticles (PMNPs) were synthesized and coated with a thermosensitive polymeric shell to obtain a synergistic effect of hyperthermia and chemotherapy.
Materials and methods: PMNPs were produced using cetyltrimethyl ammonium bromide template and then coated by a polyethylene glycol layer with molecular weight of 1500Da (PEG1500) and phase transition temperature of 48 ± 2 C to endow a thermosensitive behavior. The profile of drug release from the nanostructure was studied at various hyperthermia conditions generated by waterbath, magnetic resonance-guided focused ultrasound (MRgFUS), and alternating magnetic field (AMF). The in vitro cytotoxicity and hyperthermia efficacy of the doxorubicin-loaded nanoparticles (DOXPEG1500-PMNPs) were assessed using human lung adenocarcinoma (A549) cells.
Results: Heat treatment of DOX-PEG1500-PMNPs containing 235 ± 26mgg1 DOX at 48 C by waterbath, MRgFUS, and AMF, respectively led to 71 ± 4%, 48 ± 3%, and 74 ±5% drug release. Hyperthermia treatment of the A549 cells using DOX-PEG1500-PMNPs led to 77% decrease in the cell viability due to the synergistic effects of magnetic hyperthermia and chemotherapy.
Conclusion: The large pores generated in the PMNPs structure could provide a sufficient space for encapsulation of the chemotherapeutics as well as fast drug encapsulation and release kinetics, which together with thermosensitive characteristics of the PEG1500 shell, make DOX-PEG1500-PMNPs promising adjuvants to the magnetic hyperthermia modality.
delivery systems. In this study, porous magnetite nanoparticles (PMNPs) were synthesized and coated with a thermosensitive polymeric shell to obtain a synergistic effect of hyperthermia and chemotherapy.
Materials and methods: PMNPs were produced using cetyltrimethyl ammonium bromide template and then coated by a polyethylene glycol layer with molecular weight of 1500Da (PEG1500) and phase transition temperature of 48 ± 2 C to endow a thermosensitive behavior. The profile of drug release from the nanostructure was studied at various hyperthermia conditions generated by waterbath, magnetic resonance-guided focused ultrasound (MRgFUS), and alternating magnetic field (AMF). The in vitro cytotoxicity and hyperthermia efficacy of the doxorubicin-loaded nanoparticles (DOXPEG1500-PMNPs) were assessed using human lung adenocarcinoma (A549) cells.
Results: Heat treatment of DOX-PEG1500-PMNPs containing 235 ± 26mgg1 DOX at 48 C by waterbath, MRgFUS, and AMF, respectively led to 71 ± 4%, 48 ± 3%, and 74 ±5% drug release. Hyperthermia treatment of the A549 cells using DOX-PEG1500-PMNPs led to 77% decrease in the cell viability due to the synergistic effects of magnetic hyperthermia and chemotherapy.
Conclusion: The large pores generated in the PMNPs structure could provide a sufficient space for encapsulation of the chemotherapeutics as well as fast drug encapsulation and release kinetics, which together with thermosensitive characteristics of the PEG1500 shell, make DOX-PEG1500-PMNPs promising adjuvants to the magnetic hyperthermia modality.