Papers by Kittiphong Paiboonsukwong
Rasayan journal of Chemistry, 2021
Osteoporosis is one of the major health problems, especially considering geriatric populations. N... more Osteoporosis is one of the major health problems, especially considering geriatric populations. Nowadays, the finding of an effective and cost-efficient drug from natural medicines has been conducting. One of the strong candidates is Peperomia pellucida. Aqueous and ethanol extracts of this plant have been shown to accelerate bone healing events in vivo. However, there is no data regarding the comparison of effect from its various extracts directly on bone-related cells. The aims of this study are to observe the effect of four types of extracts with different polarity from P. pellucida on the culture of bone-related cells. Effect of the extracts on the proliferation of osteoblast cells and inhibition to osteoclastogenesis were performed in UMR-106 and RAW 264.7 cells respectively, which is induced by receptor activator of NF-kappaB ligand then analyses with TRAP Staining Assay. The determination of phytochemical compounds was performed using HPLC. The extracts can decrease the viability of both cells at a concentration 100 µg/mL. Water extract caused the morphology change of RAW 264.7 cells into M1 macrophage. No increase in osteoblast proliferation was observed. Meanwhile, each extract showed a different effect on osteoclastogenesis. Water extract successfully inhibits osteoclast formation. The activity was 92.45 ± 1.82% at a concentration of 10 µg/mL. The induction of macrophage M1 polarization was suggested as a mechanism of action. Apigenin was detected present in the extract by HPLC analysis. This study suggested the potential usage of water extract of P. pellucida as an alternative source of antiosteoporosis agents.
British Journal of Haematology
Biomedicine & Pharmacotherapy
Southeast Asian Journal of Tropical Medicine and Public Health, 2017
International Journal of Laboratory Hematology, 2020
Several DNA‐based approaches including a reverse dot‐blot hybridization (RDB) have been establish... more Several DNA‐based approaches including a reverse dot‐blot hybridization (RDB) have been established for detection of β‐thalassemia genotypes to provide accurate genetic counseling and prenatal diagnosis for prevention and control of severe β‐thalassemia. However, one of major concerns of these techniques is a risk of misdiagnosis due to a lack of DNA controls. Here, we constructed positive DNA controls for β‐thalassemia genotyping in order to ensure that all steps in the analysis are performed properly.
Biomedicine & Pharmacotherapy, 2022
The oxidative status of twenty-three β-thalassemia/hemoglobin E patients was evaluated after admi... more The oxidative status of twenty-three β-thalassemia/hemoglobin E patients was evaluated after administration of 75 mg/kg deferiprone (GPO-L-ONE®) divided into 3 doses daily for 12 months. Serum ferritin was significantly decreased; the median value at the initial and final assessments was 2842 and 1719 ng/mL, respectively. Progressive improvement with significant changes in antioxidant enzyme activity, including plasma paraoxonase (PON) and platelet-activating factor acetylhydrolase (PAF-AH), and in antioxidant enzymes in red blood cells (glutathione peroxidase (GPx), catalase and superoxide dismutase (SOD)) were observed at 3-6 months of treatment. The levels of total GSH in red blood cells were significantly increased at the end of the study. Improved red blood cell membrane integrity was also demonstrated using the EPR spin labeling technique. Membrane fluidity at the surface and hydrophobic regions of the red blood cell membrane was significantly changed after 12 months of treatment. In addition, a significant increase in hemoglobin content was observed (6.6 ± 0.7 and 7.5 ± 1.3 g/dL at the initial assessment and at 6 months, respectively). Correlations were observed between hemoglobin content, membrane fluidity and antioxidant enzymes in red blood cells. The antioxidant activity of deferiprone may partly be explained by progressive reduction of redox active iron that catalyzes free radical reactions, as demonstrated by the EPR spin trapping technique. In conclusion, iron chelation therapy with deferiprone notably improved the oxidative status in thalassemia, consequently reducing the risk of oxidative-related complications. Furthermore, the improvement in red blood cell quality may improve the anemia situation in patients.
Aging Cell, 2014
Neutrophil extracellular traps (NETs) are a recently discovered addition to the defensive armamen... more Neutrophil extracellular traps (NETs) are a recently discovered addition to the defensive armamentarium of neutrophils, assisting in the immune response against rapidly dividing bacteria. Although older adults are more susceptible to such infections, no study has examined whether aging in humans influences NET formation. We report that TNF-a-primed neutrophils generate significantly more NETs than unprimed neutrophils and that lipopolysaccharide (LPS)and interleukin-8 (IL-8)-induced NET formation exhibits a significant age-related decline. NET formation requires generation of reactive oxygen species (ROS), and this was also reduced in neutrophils from older donors identifying a mechanism for reduced NET formation. Expression of IL-8 receptors (CXCR1 and CXCR2) and the LPS receptor TLR4 was similar on neutrophils from young and old subjects, and neutrophils challenged with phorbol-12-myristate-13-acetate (PMA) showed no age-associated differences in ROS or NET production. Taken together, these data suggest a defect in proximal signalling underlies the age-related decline in NET and ROS generation. TNF-a priming involves signalling through p38 MAP kinase, but activation kinetics were comparable in neutrophils from young and old donors. In a clinical setting, we assessed the capacity of neutrophils from young and older patients with chronic periodontitis to generate NETs in response to PMA and hypochlorous acid (HOCL). Neutrophil extracellular trap generation to HOCL, but not PMA, was lower in older periodontitis patients but not in comparison with age-matched controls. Impaired NET formation is thus a novel defect of innate immunity in older adults but does not appear to contribute to the increased incidence of periodontitis in older adults.
International Journal of Laboratory Hematology
British Journal of Haematology
Frontiers in Molecular Biosciences
Uploads
Papers by Kittiphong Paiboonsukwong