Papers by Sachin O. Khairnar
Journal of Crop and Weed, Feb 1, 2021
Indian journal of animal health, Jul 4, 2023
Freshwater black molly (Poecilia sphenops) is considered one of the most important commercial orn... more Freshwater black molly (Poecilia sphenops) is considered one of the most important commercial ornamental fish due to its high popularity among aquaculturists, hobbyists, and breeders across the globe. Inland saline groundwater, akin to brackish water, with salinities ranging from 3 to 15 ppt, is abundant in India's South Western Punjab, which is now considered as the thrust area for aquaculture. The laboratory study has been designed to investigate the tolerance, survivability and behavior of black molly at different salinities i.e., 0 (control), 2, 4, 6, 8, 10, 12, 14 and 16 ppt for 10 days. Significant differences were observed in water quality variables, ionic compositions, and the survival rate of fish in all the treatments. Though the survival rate was 100% in 0 to 8 ppt, it gradually decreased from 10-16 ppt (99.29-89.72%) and drastically decreased on fourth day onwards in 12 to 16 ppt. Low swimming activity was observed from the third to fifth day onwards in 6-16 ppt. Feeding response was high to moderate in 0 to 8 ppt and moderate to less in 10-16 ppt. No threat response was observed in 0 to 8 ppt, whereas modest and weak response was noticed in 10-16 ppt. The findings of the study suggest that black molly can tolerate salinity up to 14 ppt (> 90% survival rate) and the optimum salinity range can be up to 8 ppt to achieve good swimming activity, feeding response and without any threat response.
Journal of Experimental Zoology, India, 2020
The influence of three different initial stocking densities (low stocking density [LSD] = 1.5 kg/... more The influence of three different initial stocking densities (low stocking density [LSD] = 1.5 kg/m 2 ; medium stocking density [MSD] = 3 kg/m 2 ; and high stocking density [HSD] = 6 kg/m 2) in flow-through systems was evaluated on growth and welfare in beluga, Huso huso, and ship sturgeon, Acipenser nudiventris, juveniles for 2 mo. Fish were kept in 18 concrete square tanks (2.0 × 1.0 × 1.2 m 3) at 22.3 ± 0.4 C and under a natural photoperiod. In both species, the growth performance in terms of final body weight, weight gain, specific growth rate, and feed intake significantly decreased with increasing stocking density (P < 0.05). In both species, the percent of neutrophils increased after 60 d of trial (P < 0.05). Moreover, hematocrit and white blood cell counts increased after 60 d of trial in ship sturgeon (P < 0.05). Plasma immunoglobulin significantly decreased with increasing stocking density in both species. Plasma insulin-like growth factor I decreased with increasing stocking density in beluga; however, it was not affected in ship sturgeon. Overall, these results showed that the LSD group in both species demonstrated more homogeneous and higher growth rate than the MSD and HSD groups.
Ecology, Environment and Conservation, 2023
An experimental trial was conducted in triplicate to determine the effect of different salinity l... more An experimental trial was conducted in triplicate to determine the effect of different salinity levels 0 (SA0), 2(SA2), 4(SA4), 6(SA6), 8(SA8) and 10 (SA10) ppt on survival, behaviour, and morphological changes in mrigal, Cirrhinus mrigala fingerlings during short-term rearing in inland saline water in glass aquaria for 10 days. Healthy fingerlings (average length-11.18 cm, average weight-11.05 g) of mrigal, procured from the Instructional cum Research Farm, College of Fisheries, were conditioned for one week at 0 ppt (freshwater) in FRP pools and conditioned fingerlings were gradually acclimated to varying salinities by raising the salinity by 1 ppt hourly and then stocked into glass aquaria of respective salinity levels @ 10 fingerlings aquarium-1. Predetermined salinity levels and the water level were maintained in all the treatments and fish were fed with pelleted feed (crude protein 26.12%) @ 0.5% of fish body weight, once a day, as sustenance ration throughout the experimental period. The results indicated that all the water quality parameters, except temperature, varied significantly (P0.05) across treatments. Furthermore, no fish mortality occurred up to 6 ppt salinity during the experiment. In contrast, survival rates were 93.33% and 86.67% at 8 and 10 ppt salinity, respectively at the termination of the experiment. Normal swimming and feeding (feed intake) behaviour were recorded up to 6 ppt, whereas no adverse morphological changes were observed in fish during the tolerance test in all the treatments. From the above results, it can be concluded that mrigal, C. mrigala can tolerate salinity up to 6 ppt during short term (10 days) rearing in inland saline water.
Animal Nutrition and Feed Technology, 2020
International Journal of Fisheries and Aquatic Studies, 2018
Composite farming is poly-species farming at any scale, which is found to be highly profitable be... more Composite farming is poly-species farming at any scale, which is found to be highly profitable because of the low input costs. But no longer continues, as the cost of basic inputs like cow dung, poultry manure, ground nut oil cake, mustard oil cake, rice bran and land costs has increased by many folds while the gate value of the fish remained almost constant. Quality of water, fish seed, feed and health management is essential prerequisite for optimizing production and productivity from inland fisheries and aquaculture in the country. Composite fish farming in pond culture system consists of Indian major carps (IMC) (Catla catla, Labeo rohita and Cirrhinus mrigala) and exotic major carps (Hypophthalmicthys molitrix, Ctenopharyngodon idella and Cyprinus carpio). This system is a practical alteration of the original three species IMC polyculture so as to fulfill the vacant niches in a pond ecosystem for optimizing internal resource utilization and thereby a means of increasing product...
Indian Journal of Animal Health, 2021
The present study was conducted to evaluate the minimum concentration and lethal toxicity of cast... more The present study was conducted to evaluate the minimum concentration and lethal toxicity of castor bean seed (CBS) effect on tilapia (Oreochromis mossambicus: 3.81±0.01 cm, 1.76±0.2 g) for 24 hours in glass aquaria with four treatments with three doses i.e.T1 (225 mgL-1), T2 (250 mgL-1), T3 (275 mgL-1) followed by residual effect of CBS on Amur carp (Cyprinus carpio haematopterus: 4.32±0.03 cm, 1.82±0.4 g) for 14 days in same glass aquaria with same treatments and one control (without CBS). The absolute mortality rate was recorded highest in T3 (95%) and lowest in T1 (60%). The 50% mortality was recorded within 3-4 hours of CBS powder application (ca.235 mgL-1-240 mgL-1) which can be considered as LD 50 of CBS powder. The mortality rate of Amur carp decreased below 10% after the 7 th day of CBS application in all the treatments. Water qualities were within the optimum range except for pH which decreased to acidic levels (5.7-6.8) in all the treatments. Nitrogen and phosphorus ratio in water increased with the increase of CBS doses which could be due to more nitrogen input through CBS. It is concluded that CBS can be used as a potential piscicide in aquaculture with minimal residual effects with optimum doses at 225-250 mgL-1 which subsequently will act as organic manure upon decomposition as culture progress.
Journal of Experimental Zoology, India, 2020
The present study was conducted to study the effect of molasses-fermented water hyacinth feed on ... more The present study was conducted to study the effect of molasses-fermented water hyacinth feed on growth and body composition of common carp, Cyprinus carpio. The experiment was conducted in FRP pool (1.5x1.0x0.75 m), consisted of 5 treatments (D1, D2, D3, D4 and D5) with 3 replicates each. Fermented water hyacinth (FWH) was incorporated into test diet, as an energy source, at different levels (@ 0 (D1), 40 (D2), 80(D3), 120 (D4) and 160 (D5) g/kg test diet) as a replacement for equal amount of rice bran fish were fed 3-5% of body weight for 120.All the water quality parameters were within the range for carp culture. Net weight gain (NWG) and Specific growth rate (SGR) were maximum in (24.63 g and 0.79) in D5 and minimum (16.12 g and 0.60) in D1 and the differences in NWG and SGR among treatments were significant (P≤0.05). FCR were recorded significantly (P≤0.05)better in D5 (1.18) and maximum in D1 (1.28).FWH supplementation in common carp improved flesh quality in terms of total pr...
Indian Journal of Animal Helath, 2023
Freshwater black molly (Poecilia sphenops) is considered one of the most important commercial orn... more Freshwater black molly (Poecilia sphenops) is considered one of the most important commercial ornamental fish due to its high popularity among aquaculturists, hobbyists, and breeders across the globe. Inland saline groundwater, akin to brackish water, with salinities ranging from 3 to 15 ppt, is abundant in India's South Western Punjab, which is now considered as the thrust area for aquaculture. The laboratory study has been designed to investigate the tolerance, survivability and behavior of black molly at different salinities i.e., 0 (control), 2, 4, 6, 8, 10, 12, 14 and 16 ppt for 10 days. Significant differences were observed in water quality variables, ionic compositions, and the survival rate of fish in all the treatments. Though the survival rate was 100% in 0 to 8 ppt, it gradually decreased from 10-16 ppt (99.29-89.72%) and drastically decreased on fourth day onwards in 12 to 16 ppt. Low swimming activity was observed from the third to fifth day onwards in 6-16 ppt. Feeding response was high to moderate in 0 to 8 ppt and moderate to less in 10-16 ppt. No threat response was observed in 0 to 8 ppt, whereas modest and weak response was noticed in 10-16 ppt. The findings of the study suggest that black molly can tolerate salinity up to 14 ppt (> 90% survival rate) and the optimum salinity range can be up to 8 ppt to achieve good swimming activity, feeding response and without any threat response.
Israeli journal of aquaculture-Bamidgeh, 2014
As from January 2010 The Israeli Journal of Aquaculture-Bamidgeh (IJA) will be published exclusiv... more As from January 2010 The Israeli Journal of Aquaculture-Bamidgeh (IJA) will be published exclusively as an on-line Open Access (OA) quarterly accessible by all AquacultureHub (http://www.aquaculturehub.org) members and registered individuals and institutions. Please visit our website (http://siamb.org.il) for free registration form, further information and instructions. This transformation from a subscription printed version to an on-line OA journal, aims at supporting the concept that scientific peer-reviewed publications should be made available to all, including those with limited resources. The OA IJA does not enforce author or subscription fees and will endeavor to obtain alternative sources of income to support this policy for as long as possible.
Aquatic Food Studies, Jul 28, 2022
GIFT (Genetically Improved Farmed Tilapia) mince and protein isolates obtained from pangas proces... more GIFT (Genetically Improved Farmed Tilapia) mince and protein isolates obtained from pangas processing waste (fillet frames) were used to prepare fish patties and the quality was evaluated during storage at 4°C. Patties were prepared in three lots i.e. 100% GIFT mince (T1), mince with 5% protein isolate (T2), and mince with 10% protein isolate (T3). An increase in pangas protein isolate content increased the crude protein content of patties, while it decreased the fat and moisture content (P<0.05). No significant difference was found in the color values of patties with and without protein isolates throughout the storage period. An increase in protein isolate content to 10% caused a slight decrease in cooking yield and textural quality. All the lots showed a decreasing trend in cooking yield, textural quality, and sensory scores with an increase in storage period. Patties from all the lots had no significant differences in sensory scores and were acceptable for up to 12 days during storage at 4°C. GIFT mince can be used to prepare patties with good quality, and shelf life and pangas protein isolates can be incorporated up to 10% of the mince, without affecting the quality, acceptability and shelf life.
Spanish Journal of Agricultural Research, Jun 1, 2016
has been associated with enhanced reactive oxygen species (ROS) generation (Livingstone, 2001). I... more has been associated with enhanced reactive oxygen species (ROS) generation (Livingstone, 2001). In order to counteract the potential damage resulting from excess reactive oxygen and to maintain its homeostasis, living organisms have evolved a delicate antioxidant mechanism consisting mainly of two systems: the enzyme system and the non-enzyme system. In biological research,
International Journal of Current Microbiology and Applied Sciences, Sep 10, 2018
National Academy Science Letters
Journal of Dairying, Foods & Home Sciences, Jul 13, 2022
Background: The use of probiotics in aquaculture is increasingly acknowledged, thanks to rising d... more Background: The use of probiotics in aquaculture is increasingly acknowledged, thanks to rising demand for environment friendly aquaculture. However, there is an obvious need to improve our understanding of gut microbiology, as well as the effective preparation and safety assessment of probiotics. Methods: The experiment comprised of five treatments to evaluate the influence of probiotic on water quality, growth and body composition. L. plantarum probiotic was sprayed over test diets at various concentrations @ 10 6 to 10 9 CFU/g, (D0 to D4 respectively). Water quality parameters were analysed (APHA, 2012) and growth parameters formulae (Halver, 1957) whereas fish feed and fish flesh nutritional composition, were measured (AOAC, 2012). Result: All of the water quality parameters were within acceptable limits. Net weight gain (NW G), NW G %, specific growth rate, Condition factor and survival rate were significantly (P<0.05) highest in D4 and lowest in D0. Similarly, Feed conversion ratio and protein efficiency ratio also improved significantly (P<0.05). Moreover, there was a considerable rise in crude protein and lipid content in D4. In general, supplementation of L. plantarum @ 10 9 cfu/g diet improved growth, survival and nutritional composition of Cirrhinus mrigala.
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Papers by Sachin O. Khairnar