(Q47858917)

6 February 2018

title

The preparation of 19F-labeled proteins for NMR studies (English)

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6 February 2018

1

Carl Frieden

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6 February 2018

author name string

Sydney D Hoeltzli

series ordinal

2

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6 February 2018

James G Bann

series ordinal

3

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6 February 2018

language of work or name

English

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publication date

1 January 2004

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6 February 2018

published in

Methods in Enzymology

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6 February 2018

volume

380

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6 February 2018

page(s)

400-415

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6 February 2018

61 Applications of amino acid analogs for studying co- and posttranslational modifications of proteins

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Effects of fluorine substitution on the structure and dynamics of complexes of dihydrofolate reductase (Escherichia coli)

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19F NMR spectroscopy of [6-19F]tryptophan-labeled Escherichia coli dihydrofolate reductase: equilibrium folding and ligand binding studies

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Refolding of [6-19F]Tryptophan-Labeled Escherichia coli Dihydrofolate Reductase in the Presence of Ligand: A Stopped-Flow NMR Spectroscopy Study

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Real-time and equilibrium (19)F-NMR studies reveal the role of domain-domain interactions in the folding of the chaperone PapD.

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Probing internal water molecules in proteins using two-dimensional 19F-1H NMR

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Assignment and analysis of fluorine nuclear magnetic resonance spectra of 4-fluorotryptophan myoglobins and hemoglobins

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Contribution of the tyrosines to the structure and function of the human U1A N-terminal RNA binding domain

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Fluorine-19 NMR studies of the D-galactose chemosensory receptor. 2. Calcium binding yields a local structural change

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Nuclear magnetic resonance and molecular genetic studies of the membrane-bound D-lactate dehydrogenase of Escherichia coli.

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Fluorine-19 nuclear magnetic resonance spectroscopic study of fluorophenylalanine- and fluorotryptophan-labeled avian egg white lysozymes.

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Differential effects of isomeric incorporation of fluorophenylalanines into PvuII endonuclease

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Atomic Mutations at the Single Tryptophan Residue of Human Recombinant Annexin V: Effects on Structure, Stability, and Activity‡

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Effects of divalent metal ions on the activity and conformation of native and 3-fluorotyrosine-PvuII endonucleases

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An Altered aroA Gene Product Confers Resistance to the Herbicide Glyphosate

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Side chain accessibility and dynamics in the molten globule state of alpha-lactalbumin: a (19)F-NMR study

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Role of entropy in protein thermostability: folding kinetics of a hyperthermophilic cold shock protein at high temperatures using 19F NMR.

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The specific incorporation of labelled aromatic amino acids into proteins through growth of bacteria in the presence of glyphosate. Application to fluorotryptophan labelling to the H(+)-ATPase of Escherichia coli and NMR studies.

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Dynamic NMR spectral analysis and protein folding: identification of a highly populated folding intermediate of rat intestinal fatty acid-binding protein by 19F NMR

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Expression and nitrogen-15 labeling of proteins for proton and nitrogen-15 nuclear magnetic resonance

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Expansion of the genetic code: Site‐directed p‐fluoro‐phenylalanine incorporation in Escherichia coli

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Biosynthesis of a highly stable coiled-coil protein containing hexafluoroleucine in an engineered bacterial host

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Fluoroprolines as Tools for Protein Design and Engineering We thank Mrs. E. Weyher for skillful technical assistance in spectroscopic analyses and Mrs. W. Wenger for her excellent technical assistance in protein preparation. We are indebted to Dr. R

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7 January 2021

Biochemistry: biosynthesis of an organofluorine molecule

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7 January 2021

Expanding the genetic code of Escherichia coli

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7 January 2021

Solution 19F nuclear Overhauser effects in structural studies of the cytoplasmic domain of mammalian rhodopsin

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7 January 2021

NMR spectroscopy in studies of light-induced structural changes in mammalian rhodopsin: applicability of solution (19)F NMR.

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7 January 2021

Amino acid replacements of the glutamic acid residue at position 48 in the tryptophan synthetase A protein of Escherichia coli

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7 January 2021

19F NMR of trifluoroacetyl-labeled cysteine mutants of myoglobin: structural probes of nitric oxide bound to the H93G cavity mutant.

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7 January 2021

Topology and secondary structure of the N-terminal domain of diacylglycerol kinase.

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Thiol as an electron donor in molybdenum oxo-transferase analog reaction systems: observations by fluorine-19 NMR spectroscopy and biological implications

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7 January 2021

Energetics of the equilibrium between two nucleotide-free myosin subfragment 1 states using fluorine-19 NMR

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7 January 2021

Mobility of the N-terminal segment of rabbit skeletal muscle F-actin detected by 1H and 19F nuclear magnetic resonance spectroscopy

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7 January 2021

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Identifiers

DOI

1 reference

6 February 2018

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6 February 2018