(Q40789130)

English

In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs

scientific article published on August 15, 1995

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PMC publication ID

23 September 2017

In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs (English)

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23 September 2017

Reinhard Georg Lührmann

4

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Lührmann R

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23 September 2017

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V. Ségault

1

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30 July 2024

C. L. Will

2

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A7664740

30 July 2024

B. S. Sproat

3

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A7664740

30 July 2024

R. Lührmann

4

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30 July 2024

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publication date

1 August 1995

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23 September 2017

15 August 1995

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=7664740

30 July 2024

full work available at URL

https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/j.1460-2075.1995.tb00072.x

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A7664740

30 July 2024

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/7664740/?tool=EBI

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A7664740

30 July 2024

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/7664740/pdf/?tool=EBI

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A7664740

30 July 2024

https://europepmc.org/articles/PMC394479

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A7664740

30 July 2024

https://europepmc.org/articles/PMC394479?pdf=render

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Europe PubMed Central

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A7664740

30 July 2024

https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fj.1460-2075.1995.tb00072.x

1 reference

10.1002/J.1460-2075.1995.TB00072.X

https://api.crossref.org/works/10.1002/J.1460-2075.1995.TB00072.X

30 July 2024

http://onlinelibrary.wiley.com/wol1/doi/10.1002/j.1460-2075.1995.tb00072.x/fullpdf

1 reference

10.1002/J.1460-2075.1995.TB00072.X

https://api.crossref.org/works/10.1002/J.1460-2075.1995.TB00072.X

30 July 2024

The EMBO Journal

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Europe PubMed Central

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23 September 2017

14

1 reference

Europe PubMed Central

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23 September 2017

16

1 reference

Europe PubMed Central

PMC publication ID

23 September 2017

4010-4021

1 reference

Europe PubMed Central

PMC publication ID

23 September 2017

The determinants for Sm protein binding to Xenopus U1 and U5 snRNAs are complex and non-identical.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Small nuclear ribonucleoprotein (RNP) U2 contains numerous additional proteins and has a bipartite RNP structure under splicing conditions

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Evidence that the 60-kDa protein of 17S U2 small nuclear ribonucleoprotein is immunologically and functionally related to the yeast PRP9 splicing factor and is required for the efficient formation of prespliceosomes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Human snRNP polypeptide D1 promotes pre-mRNA splicing in yeast and defines nonessential yeast Smd1p sequences

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Yeast protein splicing factors involved in nuclear pre-mRNA splicing

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Protein composition of mammalian spliceosomal snRNPs

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

m3G cap hypermethylation of U1 small nuclear ribonucleoprotein (snRNP) in vitro: evidence that the U1 small nuclear RNA-(guanosine-N2)-methyltransferase is a non-snRNP cytoplasmic protein that requires a binding site on the Sm core domain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Pseudouridine formation in U2 small nuclear RNA.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Mutations in an essential U2 small nuclear RNA structure cause cold-sensitive U2 small nuclear ribonucleoprotein function by favoring competing alternative U2 RNA structures

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

The association of the U1-specific 70K and C proteins with U1 snRNPs is mediated in part by common U snRNP proteins

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Nucleo-cytoplasmic transport of U snRNPs: definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

U2 RNA shares a structural domain with U1, U4, and U5 RNAs

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Functional analysis of mutant Xenopus U2 snRNAs

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

In vitro reconstitution of functional yeast U2 snRNPs

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

U2 snRNA sequences that bind U2-specific proteins are dispensable for the function of U2 snRNP in splicing

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Structure of spliceosomal snRNPs and their role in pre-mRNA splicing

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Electron microscopy of small nuclear ribonucleoprotein (snRNP) particles U2 and U5: evidence for a common structure-determining principle in the major U snRNP family

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Biochemical Mechanisms of Constitutive and Regulated Pre-mRNA Splicing

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Pseudouridine modification of U5 RNA in ribonucleoprotein particles assembled in vitro

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Evidence for three distinct D proteins, which react differentially with anti-Sm autoantibodies, in the cores of the major snRNPs U1, U2, U4/U6 and U5

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Reconstitution of functional mammalian U4 small nuclear ribonucleoprotein: Sm protein binding is not essential for splicing in vitro

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

3'-terminal labelling of RNA with T4 RNA ligase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

24 September 2017

Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

25 September 2017

A 69-kD protein that associates reversibly with the Sm core domain of several spliceosomal snRNP species.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

15 June 2018

A compensatory base change in human U2 snRNA can suppress a branch site mutation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

15 June 2018

In vitro assembly of yeast U6 snRNP: a functional assay

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

15 June 2018

In vitro reconstitution of snRNPs: a reconstituted U4/U6 snRNP participates in splicing complex formation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

15 June 2018

Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

15 June 2018

Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural element

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

15 June 2018

Reconstituted mammalian U4/U6 snRNP complements splicing: a mutational analysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

15 June 2018

U2 small nuclear RNA 3' end formation is directed by a critical internal structure distinct from the processing site

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

19 August 2018

Phylogenetic conservation of modified nucleotides in the terminal loop 1 of the spliceosomal U5 snRNA

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

19 August 2018

Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=394479

19 August 2018

Conformational changes of U6 RNA during the spliceosome cycle: an intramolecular helix is essential both for initiating the U4-U6 interaction and for the first step of slicing

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Interaction of the human autoantigen p150 with splicing snRNPs

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Purification of small nuclear ribonucleoprotein particles with antibodies against modified nucleosides of small nuclear RNAs

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Formation of pseudouridine in U5 small nuclear RNA

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Isolation of small nuclear ribonucleoproteins containing U1, U2, U4, U5, and U6 RNAs

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavage

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing

1 reference

https://pubmed.ncbi.nlm.nih.gov/7664740

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1002/J.1460-2075.1995.TB00072.X

1 reference

National Center for Biotechnology Information

PubMed publication ID

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=7664740

30 July 2024

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

23 September 2017

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

23 September 2017

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