(Q34497847)

2 August 2017

title

The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli (English)

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2 August 2017

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1 reference

Chris R Reisch

1

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2 August 2017

Kristala L J Prather

series ordinal

2

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2 August 2017

language of work or name

English

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publication date

14 October 2015

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2 August 2017

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2 August 2017

volume

5

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2 August 2017

page(s)

15096

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Creative Commons Attribution 4.0 International

2 August 2017

copyright license

start time

14 October 2015

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April 2022 Public Data File from Crossref

copyright status

copyrighted

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cites work

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

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Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system

7 July 2018

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Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites

7 July 2018

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Genome editing. The new frontier of genome engineering with CRISPR-Cas9

7 July 2018

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A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica.

7 July 2018

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CRISPR-Cas9-assisted recombineering in Lactobacillus reuteri

7 July 2018

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Harnessing CRISPR-Cas9 immunity for genetic engineering

7 July 2018

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Conditional DNA repair mutants enable highly precise genome engineering

7 July 2018

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Programmable removal of bacterial strains by use of genome-targeting CRISPR-Cas systems

7 July 2018

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Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli

7 July 2018

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DNA targeting specificity of RNA-guided Cas9 nucleases

7 July 2018

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Cytotoxic chromosomal targeting by CRISPR/Cas systems can reshape bacterial genomes and expel or remodel pathogenicity islands

7 July 2018

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Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression

7 July 2018

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RNA-guided editing of bacterial genomes using CRISPR-Cas systems

7 July 2018

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7 July 2018

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Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases

7 July 2018

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Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering.

7 July 2018

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A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

7 July 2018

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Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection

7 July 2018

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Genome-scale promoter engineering by coselection MAGE

7 July 2018

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Precise manipulation of chromosomes in vivo enables genome-wide codon replacement

7 July 2018

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Multiplexed genome engineering and genotyping methods applications for synthetic biology and metabolic engineering

7 July 2018

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Preparation of highly efficient electrocompetent Escherichia coli using glycerol/mannitol density step centrifugation

7 July 2018

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Probing Cellular Processes with Oligo-Mediated Recombination and Using the Knowledge Gained to Optimize Recombineering

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Single-stranded heteroduplex intermediates in lambda Red homologous recombination

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Automated design of synthetic ribosome binding sites to control protein expression

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Circular polymerase extension cloning of complex gene libraries and pathways

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Programming cells by multiplex genome engineering and accelerated evolution

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Enzymatic assembly of DNA molecules up to several hundred kilobases

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Recombineering: a homologous recombination-based method of genetic engineering

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

A simple and fast method for determining colony forming units

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Rapid and efficient construction of markerless deletions in the Escherichia coli genome

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(R)/ET(R) Recombination

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Making ends meet: repairing breaks in bacterial DNA by non-homologous end-joining

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosome

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Spectra of spontaneous mutations in Escherichia coli strains defective in mismatch correction: the nature of in vivo DNA replication errors.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Mapping and sequencing of mutations in the Escherichia colirpoB gene that lead to rifampicin resistance

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS)

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4604488

Synergy between tetA and rpsL provides high-stringency positive and negative selection in bacterial artificial chromosome vectors

7 July 2018

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FSREP15096

Bacterial DNA polymerases participate in oligonucleotide recombination

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FSREP15096

21 January 2018

Identifiers

DOI

10.1038/SREP15096

1 reference

Dimensions Publication ID

2 August 2017

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1 reference

2 August 2017

1 reference