(Q101104970)
Statements
香蕉果实SMART cDNA文库的构建及利用PCR方法筛选香蕉Actin2基因 (Chinese)
The Construction of SMART cDNA Library from Banana Fruit and the Screening of Actin2 Gene by Using PCR Method (English)
徐碧玉/Xu BiYu
苏伟/Su Wei
张建斌/Zhang JianBin
刘菊华/Liu JuHua
金志强/Jin ZhiJiang*
利用SMART(switching mechanism at5'end of RNA transcript)技术,提取果实少量总RNA,经15-25轮LD-PCR扩增获得全长ds-cDNA,构建了海南主栽的食用香蕉巴西蕉(MusaAAA Group Cavendish)果实的cDNA文库.所构建的文库容量为5×106 Pfu ml-1,重组率93%.利用此cDNA文库,采用96孔板PCR法筛选香蕉Actin2基因,测序结果显示,序列全长1723 bp,编码区长1… (Chinese)
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In this paper, SMART （switching mechanism at 5＇ end of RNA transcript）was used in the construction of cDNA library of postharvested banana fruit. (English)
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