(Q101104390)

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RAPD MAKERS FOR HERMAPHRODITE GENE IN PAPAYA

scientific article published in 2001

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Bulked segregant analysis was used to identify RAPD markers linked to the hermaphrodite gene (M2) in papaya (Carica papaya L.). (English)
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应用混合分离群体分析法, 在205个10碱基随机引物中, 寻找番木瓜两性基因(M2)的RAPD标记;发现两个多态性RAPD条带:OPQ 071800和OPE 061050,它们仅能在两性DNA池中扩增到,而不能在雌性DNA池中扩增到.用上述两引物检测了210株番木瓜单株DNA, 结果表明, OPQ 071800和OPE 061050与M2基因紧密连锁,它们位于M2基因的两侧,其遗传距离分别为4.5和1.9 cM.. (Chinese)
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