Papers by Dr. Mysoon Al-Ansari
Process safety and environmental protection/Transactions of the Institution of Chemical Engineers. Part B, Process safety and environmental protection/Chemical engineering research and design/Chemical engineering research & design, May 1, 2024
Polymers for advanced technologies, May 1, 2024
Polymers for advanced technologies, Apr 1, 2024
Cells
The genetic architecture of mitochondrial disease continues to expand and currently exceeds more ... more The genetic architecture of mitochondrial disease continues to expand and currently exceeds more than 350 disease-causing genes. Bi-allelic variants in RTN4IP1, also known as Optic Atrophy-10 (OPA10), lead to early-onset recessive optic neuropathy, atrophy, and encephalopathy in the afflicted patients. The gene is known to encode a mitochondrial ubiquinol oxidoreductase that interacts with reticulon 4 and is thought to be a mitochondrial antioxidant NADPH oxidoreductase. Here, we describe two unrelated consanguineous families from the northern region of Saudi Arabia harboring a missense variant (RTN4IP1:NM_032730.5; c.475G
Chemosphere, Apr 1, 2022
Arsenic (As) contamination in an ecosystem has been a serious threat for the ecosystem as well as... more Arsenic (As) contamination in an ecosystem has been a serious threat for the ecosystem as well as human health. Thus, the present study was established an eco-friendly remediation of As by using As resistant Rhodobacter sp. Accordingly, the growth of Rhodobacter sp. in As stress environment was assessed. Expectedly, enhanced growth order of the Rhodobacter sp., under As stress was found to be control >50 > 100 > 200 > 300 > 400 > 500 mg/L of As. In addition, the present study explored the influence of various light sources (Yellow, light blue, red, green and white) on growth and As removal mechanisms of Rhodobacter sp. The growth profile of the bacteria indicated that the light blue source showed an enhanced growth at 72 h of incubation. Based on optimization experiments, an increased As removal percentage rate was found to be at 87.5% at pH 7.0, 3% of glucose, 1% of citrate supplemented in the medium. The As resistant genetic pattern for arsenic transformation, the genes arsenate reductase (arsC), arsenite oxidase (aio) was investigated. To study the transcript level expression of arsC and aio genes were performed after exposure to different concentrations of As (50, 100, 150, and 200 mg/L) at different time intervals (24, 48, 72 and 96 h). The results showed that both arsC and aio were up regulated from 24-72 h and the down regulation was observed at 96 h. The obtained results indicated that the Rhodobacter sp., possess significant AS tolerance and removal potential would make it is a noteworthy candidate for future As remediation practices.
Chemosphere, 2022
Arsenic (As) contamination in an ecosystem has been a serious threat for the ecosystem as well as... more Arsenic (As) contamination in an ecosystem has been a serious threat for the ecosystem as well as human health. Thus, the present study was established an eco-friendly remediation of As by using As resistant Rhodobacter sp. Accordingly, the growth of Rhodobacter sp. in As stress environment was assessed. Expectedly, enhanced growth order of the Rhodobacter sp., under As stress was found to be control >50 > 100 > 200 > 300 > 400 > 500 mg/L of As. In addition, the present study explored the influence of various light sources (Yellow, light blue, red, green and white) on growth and As removal mechanisms of Rhodobacter sp. The growth profile of the bacteria indicated that the light blue source showed an enhanced growth at 72 h of incubation. Based on optimization experiments, an increased As removal percentage rate was found to be at 87.5% at pH 7.0, 3% of glucose, 1% of citrate supplemented in the medium. The As resistant genetic pattern for arsenic transformation, the genes arsenate reductase (arsC), arsenite oxidase (aio) was investigated. To study the transcript level expression of arsC and aio genes were performed after exposure to different concentrations of As (50, 100, 150, and 200 mg/L) at different time intervals (24, 48, 72 and 96 h). The results showed that both arsC and aio were up regulated from 24-72 h and the down regulation was observed at 96 h. The obtained results indicated that the Rhodobacter sp., possess significant AS tolerance and removal potential would make it is a noteworthy candidate for future As remediation practices.
Endocrine-related Cancer, Apr 1, 2021
The prognosis for lung cancer patients treated with chemotherapy is poor. Single nucleotide polym... more The prognosis for lung cancer patients treated with chemotherapy is poor. Single nucleotide polymorphisms (SNPs) in matrix metalloproteinase (MMP) genes could influence treatment outcome by altering apoptotic pathways. Eight SNPs with known or suspected phenotypic effect in six genes (MMP1, MMP2, MMP3, MMP7, MMP9 and MMP12) were investigated. For 349 Caucasian patients with primary lung cancer, receiving first-line chemotherapy, three different endpoints were analysed: response after the second cycle, progression free survival (PFS) and overall survival (OS). The prognostic value of the SNPs was analysed using multiple logistic regression for all patients and histology-, stage-and treatment-specific subgroups. Hazard ratio estimates for PFS and OS were calculated using Cox regression methods. None of the investigated polymorphisms modified response significantly in the whole patient population. However, tumour stage IIIB variant allele carriers of MMP2 C-735T showed a significantly worse response. PFS was significantly prolonged in MMP1 G-1607GG variant allele carriers and OS in small cell lung cancer patients carrying the MMP12 A-82G variant allele. In conclusion, this study identified SNPs in MMP1, MMP2, MMP7 and MMP12 for further investigation as possible predictors of chemotherapy outcome in lung cancer patients.
International Journal of Molecular Sciences, Sep 5, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
International Journal of Molecular Sciences
Breast cancer is the most prevalent form of cancer among women. The microenvironment of a cancer ... more Breast cancer is the most prevalent form of cancer among women. The microenvironment of a cancer tumor is surrounded by various cells, including the microbiota. An imbalance between microbes and their host may contribute to the development and spread of breast cancer. Therefore, the objective of this study is to investigate the influence of Enterococcus faecalis on a breast cancer cell line (MCF-7) to mimic the luminal A subtype of breast cancer, using an untargeted proteomics approach to analyze the proteomic profiles of breast cancer cells after their treatment with E. faecalis in order to understand the microbiome and its role in the development of cancer. The breast cancer cell line MCF-7 was cultured and then treated with a 10% bacterial supernatant at two time points (24 h and 48 h) at 37 °C in a humidified incubator with 5% CO2. Proteins were then extracted and separated using two-dimensional difference (2D-DIGE) gel electrophoresis, and the statistically significant proteins...
Environmental Research, Jun 1, 2023
International Journal of Molecular Sciences, Feb 20, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Microbiological Research, Dec 1, 2022
Journal of King Saud University - Science, Apr 1, 2022
Cells, Oct 25, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Environmental Research, May 1, 2023
Applied Nanoscience, Feb 11, 2022
The present study was emphasized to investigate the photocatalytic degradation of malachite green... more The present study was emphasized to investigate the photocatalytic degradation of malachite green (MG) and antibacterial activity of zirconium oxide nanoparticles synthesized from Annona reticulata leaf extract (AR-ZrO2NPs). The biologically synthesized AR-ZrO2NPs were characterized by UV–visible spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and Field emission scanning electron microscope (FESEM). The UV–visible spectrum of AR-ZrO2NPs showed a characteristic surface plasmon resonance (SPR) peak at 256 nm. The XRD analysis exhibited that the AR-ZrO2NPs were crystalline in nature. FT-IR revealed that the AR leaf extract has stabilized with the nanoparticles by a capping agent. In addition, electron microscopic results revealed that the AR-ZrO2NPs were spherical in shape and found to be 13–20 nm range in size. The biologically produced AR-ZrO2NPs have antibacterial action against Salmonella enterica serotype typhi, which is multi-drug-resistant. Furthermore, AR-ZrO2NPs demonstrated exceptional degrading efficiency for MG, with about 87.4% elimination in 150 min of sunshine irradiation. As a result, this research concludes that AR leaf extract is a possible green resource for AR-ZrO2NPs synthesis with multi-potential applications.
Applied Nanoscience, Sep 25, 2021
In the present study, we report a simple, rapid, cost-effective approach for the white synthesis ... more In the present study, we report a simple, rapid, cost-effective approach for the white synthesis of silver nanoparticles (AgNPs) using Capra aegagrus hircus milk. The formation of AgNPs was visually examined and further investigated using UV–visible spectrophotometer, transmission electron microscopy, scanning electron microscopy with energy dispersive X-ray, Fourier infrared spectroscopy, and X-ray diffractometer. Crystalline lattice indices of AgNPs were performed using the XRD analysis. The diffraction peaks at 2θ values of 37.7°, 46.1°, 67.4°, and 76.84° corresponding to lattice planes (111), (200), (220), and (311), respectively. The obtained AgNPs were spherical in shape with the size between 5 and 50 nm. The antibacterial activity of AgNPs against Klebsiella sp. (Accession Number: KC899845), and Staphylococcus sp. (Accession Number: KC688883) were evaluated by means of cell growth.
Journal of Drug Delivery Science and Technology, Feb 1, 2021
Abstract Green synthesized silver nanoparticles (AgNPs) are expected to have rich biomedical appl... more Abstract Green synthesized silver nanoparticles (AgNPs) are expected to have rich biomedical applications as an alternative medicine for health care, with the bioactive compounds of plants acting as moderating agents in the synthesis of silver nanoparticles with high therapeutic potential. In this study, frankincense (also known as Al-liban in Arabic), which is a resin obtained from the bark of the tree Boswellia carterii and a traditional alternative medicine, was used as a moderating agent. The synthesized silver nanoparticles [BW-AgNPs] were characterized using UV–vis, EDAX, FT-IR, and SEM analyses. The antimicrobial activities of the synthesized nanoparticles were tested against Streptococcus mutans, Pseudomonas aeruginosa, Enterococcus faecalis, and Candida albicans. Additionally, the anti-S. mutans activities were traced at the molecular level; the impact of the nanoparticles on eight functional genes, brpA, comDE, spaP, smu360, gtfB, gtfCc, gtfDd and gbpB, was probed; and the minimum inhibitory concentration was quantified using microdilution and resazurin staining techniques. The diameter of the zone of inhibition for S. mutans was 22.0 mm at 200 μg/NP treatment, and the SEM study showed that the NPs were irregular to spherical. The size range of the synthesized NPs was 14.19–85.36 nm, with an average of 14.8 ± 0.3 nm based on the TEM study. The FTIR spectra of crude frankincense and frankincense-mediated AgNPs showed peak bands with closely related wavelengths, which indicates that frankincense participates as capping and surface-attached components in the NPs. The NPs showed high antibacterial action against the oral pathogen S. mutans, which causes dental caries. This finding may promote the development of commercial products that incorporate NPs, such as toothpaste and mouth wash. NPs in a size range of less than 30 nm have been reported to easily target SARS-CoV-2; thus, the AgNPs synthesized here with a size range of 14.188–15.202 nm may pave the way for further research.
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Papers by Dr. Mysoon Al-Ansari