Papers by ramovatar meena
Oxidative Medicine and Cellular Longevity
Cadmium selenium quantum dots (CdSe QDs) with modified surfaces exhibit superior dispersion stabi... more Cadmium selenium quantum dots (CdSe QDs) with modified surfaces exhibit superior dispersion stability and high fluorescence yield, making them desirable biological probes. The knowledge of cellular and biochemical toxicity has been lacking, and there is little information on the correlation between in vitro and in vivo data. The current study was carried out to assess the toxicity of CdSe QDs after intravenous injection in Wistar male rats (230 g). The rats were given a single dose of QDs of 10, 20, 40, and 80 mg/kg and were kept for 30 days. Following that, various biochemical assays, hematological parameters, and bioaccumulation studies were carried out. Functional as well as clinically significant changes were observed. There was a significant increase in WBC while the RBC decreased. This suggested that CdSe quantum dots had inflammatory effects on the treated rats. The various biochemical assays clearly showed that high dose induced hepatic injury. At a dose of 80 mg/kg, bioaccu...
Journal of Functional Biomaterials
Considerable attention has been given to Magnesium oxide nanoparticles lately due to their antimi... more Considerable attention has been given to Magnesium oxide nanoparticles lately due to their antimicrobial potential, low toxicity to humans, high thermal stability, biocompatibility, and low cost of production. However, their successful transformation into sustainable drugs is limited due to their low membrane permeability, which reduces their bioavailability in target cells. Herein we propose Cerium-doped magnesium oxide nanoparticles (MgOCeNPs) as a powerful solution to above mentioned limitations and are compared with MgO NPs for their membrane permeability and antimicrobial activity. Both pure and Ce-doped were characterized by various spectroscopic and microscopic techniques, in which an X-ray diffraction (XRD) examination reveals the lattice patterns for doped nanoparticles. Furthermore, Atomic Force Microscopy (AFM) revealed the three-dimensional (3D) structure and height of the nanoparticle. The crystal structure (FCC) of MgO did not change with Ce doping. However, microstruc...
Applied Biochemistry and Biotechnology, 2022
Betel leaf is consumed as a mouth freshener due to its characteristic flavor, aromaticity, and me... more Betel leaf is consumed as a mouth freshener due to its characteristic flavor, aromaticity, and medicinal values. Abundance of phytochemicals in betel leaf contributes towards unique qualitative features. Screening of metabolites is quintessential for identifying flavoring betel leaves and their origin. Metabolomics presently lays emphasis on the cumulative application of gas chromatography–mass spectrometry and nuclear magnetic resonance spectroscopic approaches. Here we adopted different protocols based on the above-mentioned analytical metabolomics platform for untargeted plant metabolite profiling followed by multivariate analysis methods and a phytochemical characterization of Piper betel leaf cultivars endemic to coastal Odisha, India. Based on variation in the solvent composition, concentration of solvent, extraction temperature, and incubation periods, five extraction methods were followed in GC–MS and NMR spectroscopy of betel leaf extracts. Phytochemical similarities and differences among the species were characterized through multivariate analysis approaches. Principal component analysis, based on the relative abundance of phytochemicals, indicated that the betel cultivars could be grouped into three groups. Our results of FTIR-, GC–MS-, and NMR-based profiling combined with multivariate analyses suggest that untargeted metabolomics can play a crucial role in documenting metabolic signatures of endemic betel leaf varieties.
Treatment: L-BPA and fructose were dissolved in disstileed water at a molar ratio of 1:1.5, and t... more Treatment: L-BPA and fructose were dissolved in disstileed water at a molar ratio of 1:1.5, and then a 1.15 M ratio of 1 N NaOH was added. The mixture was stirred until L-BPA had completely dissolved, and the pH value was titrated to 7.6 with 1 N HCl. the solution was filtered through a 0.22 μm syringe filter for sterilization (Merck Millipore, Massachusetts, USA). The final concentration was set to 30 mg/mL. Tumor volumes were calculated using the formula π/6 x Length x Width x Height. Treatments were started when the tumor reached a volume of 100-150 mm3 in the B16-Luc model and 150-220 mm3 in the SCC-VII model. For BNCT group, 500 mg/kg of BPA was subcutaneously injected into mice into the nuchal sites. Tumor was irradiated by thermal and epi-thermal neutron (5MW, Institutes for Integrated Radiation and Nuclear Science, Kyoto University) 45 min after BPA injection for 15 min. For in vivo depletion of CD8+ T cells, CD8+ cell-depleting antibody was injected intraperitoneally(i.p.) ...
Elemental analysis of soils and leafs is widely employed to quantify the fertilizer and nutrients... more Elemental analysis of soils and leafs is widely employed to quantify the fertilizer and nutrients imbalance pertaining to deficit in agricultural productivity. EDXRF methodology offers an ideal technique to evaluate the direct detection of dried powdered leaf samples. This strategy enabled the concurrent determination of Mg2+, Ca 2+, P-3, K+, Fe2+, Cu2+, B-1, Si+1, Al+3, Ti+4, Mn+4 etc. This present study discriminates depositional behavior of macro minerals like P, S leaf varieties of Bali, Chandrakana and Jhanji. However, Chandrakana variety promotes highest micro mineral accumulation and Jhanji one the least. Vineyard sandy soils of BLS and BUS impart more MnO as compared to clayey soils of PLS and PUS. It also reflects high abundance of Cl in clayey soils relative to sandy soils. Thus, the present findings promote EDXRF employment for direct detection of agricultural samples. The outcomes also motivate further studies for trialing other crops for nutrition and deficiency analysis.
The Piper betle L. leaves and their significance were described in various ayurvedic studies of I... more The Piper betle L. leaves and their significance were described in various ayurvedic studies of India and China for its diverse use in cultural practices and treatment of various health disorders. The leaves of P. betle were used as post-meal mouth freshener in India for centuries. However, it offers economic benefits to farmers of Coastal India at large. Betel leaves cultivated agricultural soils play a significant role for their mineralogical composition. So, this present study aimed to find out the soil physicochemical characteristics and C/N contents of Betel vineyards of coastal Odisha. The soil and water samples were collected from local varieties of P. betle L. cultivated vineyards of Balasore, Ganjam, and Puri districts of Odisha and investigated their mineralogical composition. The soil mineralogy plays crucial role to understand the soil-plant relations. Coastal soil samples also contain the most prized mineral aggregations from economical perspectives. The mineralogical composition involves chemical composition, essential elemental composition, and surface morphology. The mineralogical and elemental composition of soil samples were carried out by using various techniques like Fourier transform infrared spectroscopy, X-ray diffraction (XRD) and energy-dispersive X-ray fluorescence, scanning electron microscopy attached with energy-dispersive X-ray system. CHNS analyzer for quantification of hydrogen, nitrogen, carbon, and sulfur content. H 2 O 2 (30%)-treated soil is employed to eliminate the organic carbon from mass soil samples. The scanning electron microscopy analysis revealed the presence of heterogeneity shape and size of surface soils of both treated and untreated soils. For estimation of total organic carbon (TOC), inorganic carbon, total nitrogen, and total carbon, water samples were analyzed through TOC analyzer. Percentage variation arises in GAN and PUR sites soil due to more assumption of organic matter from clay soils in comparison with sandy soils of BAL. The spectra of FTIR point out Kaolinite and Quartz as the key components and others are minor components. The common minerals like quartz, hematite, kaolinite, montmorillonite, calcite, organic matter and illite in diverse compositions are recognized. Further, the presence of these above minerals was confirmed by the XRD analysis. Morphological analysis of kaolinite indicated euhedral, hexagonal, and pseudo-hexagonal-shaped plates. The mineralogical data revealed the relative abundance of phosphorus and nitrogen was less in all soils. Depletion of P and N may be resulted due to introduction of fresh plowed soil from grazed pastoral land. The present research uncovers that soils requires adequate input of additional compost, manures, and fertilizers for maximal vegetative growth and economic yield as well. As P. betle species is very precious medicinal plant, and this research suggested not to use contaminated water for betel cultivation. Our results also helpful for the improvement in soil management in the vineyards to determining the mineral nutrients that affect plant growth and development.
Environmental Science and Pollution Research, 2021
Advancements in telecommunication sector result in increasing exposure to electromagnetic (EM) ra... more Advancements in telecommunication sector result in increasing exposure to electromagnetic (EM) radiation, which has been correlated with incidence of male infertility. Therefore, the present study focused on analyzing the consequence of EM radiation (2115 MHz) exposure on the reproductive system of male Wistar rats. Besides, the antioxidant protective effect of Punica granatum juice was also evaluated. For experimental analysis, rats were divided into five groups (control, sham exposed, exposed, herbal plus exposed, and herbal only). Individual group consisted of 6 rats which were exposed to radiation for 45 days (2 h/day). The herbal-treated groups were given 1 ml of Punica granatum extract orally. Various parameters such as organ to body ratio, sperm count, motility, viability, and testis histopathology were studied. Furthermore, oxidative stress parameters and free radical generation were analyzed. The exposed group showed changes in sperm parameters along with decrease in seminiferous tubule diameter. On the contrary, herbal-exposed group showed enhanced sperm count, increased motility, and viability in comparison to exposed group. Histopathology studies also revealed the protective role of herbal juice. Significant alteration in oxidative parameters along with an enhanced free radical generation in exposed group and reduction in herbal groups was observed. The results thus indicate that continuous exposure to EM radiation can lead to oxidative stress which induces biochemical changes in rat sperms. However, Punica granatum extract has a protective role against oxidative damage induced by EM radiation.
Journal of Cluster Science, 2020
Boron nitride (BN) has encouraged researchers to use it in composite materials due to its differe... more Boron nitride (BN) has encouraged researchers to use it in composite materials due to its different properties. BN-based nanocomposites have been synthesized using a modified chemical method. X-ray diffraction (XRD) results confirmed the formation of hexagonal-BN and nickel oxide (NiO) phases in samples. XRD analysis shows that samples are textured along (100) plane which distorts structure and leads to formation of defect states. The electron density difference between BN and NiO as well as textural growth are two main reasons for variation in diffraction peak intensities. Raman spectroscopic results also revealed that peak intensity and position shifts with increase in NiO content, due to the distortions induced by NiO incorporation confirming the presence of defect states. Field emission scanning electron microscopy (FESEM) results showed that morphology of synthesized nanocomposites varies with increase of NiO concentration. Ultraviolet (UV)visible results revealed that the absorption edge is red-shifted with NiO content, consequently, the band-gap energy of composites get decreased. Distorted crystal structure and defect states are responsible for variation in structural, morphological as well as optical properties. Results suggested that the nanocomposites can be explored for photodetection as well as gas sensing applications.
Journal of Cluster Science, 2020
Boron carbide enriched with 10 B isotope (10 B 4 C) was synthesized by simple and low cost solvot... more Boron carbide enriched with 10 B isotope (10 B 4 C) was synthesized by simple and low cost solvothermal method. Structural, morphological and optical analysis has been done. Hexagonal phase of the synthesized material show emission in visible region and makes it a promising material for cancer diagnosis. Cytotoxicity analysis has been done to analyze its suitability for biomedical applications. Boron neutron capture therapy (BNCT) has been performed using thermal neutron source in comparison to clinically studied compound on tumor cell lines (HeLa and U-87MG).
Journal of Cluster Science, 2019
In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Ponga... more In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Pongamia pinnata (PLE) and characterized using various analytical techniques such as UV–visible spectroscopy, TEM, SEM, EDX, and XRD. UV–Visible spectra showed surface plasmon resonance (SPR) peaks in the range of 520–540 nm signifying the synthesis of colloidal AuNPs stabilised by PLE. Further, we investigated the cytotoxic effects of AuNPs against human cervical cancer cell line (HeLa). MTT assay screened IC50 value of 200 μg/mL at 24 h for AuNPs formed at varying HAuCl4 concentration. Flow cytometry measurements using H2DCFDA showed that the toxicity of AuNPs was attributed to the generation of reactive oxygen species (ROS). Besides, the in vitro anticancer activities of AuNPs were studied in HeLa cells by several assays which resulted in altered cell morphology, reduced potential of wound healing, inactive mitochondria due to loss of mitochondrial membrane potential (MMP) and cell cycle arrest. AuNPs showed dose-dependent selective toxicity towards HeLa cells and were non-toxic against human embryonic kidney cell line (HEK293). The study revealed an efficient, ecofriendly and simple method for synthesis of multifunctional AuNPs using green synthetic approach with high anticancer potential for cervical cancer cell line.
Materials Letters, 2019
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Journal of Cluster Science, 2019
Plant-based photo fabrication of noble metal nanoparticles is a dominating research area in nanob... more Plant-based photo fabrication of noble metal nanoparticles is a dominating research area in nanobiotechnology as they offer immense applications in biomedicine, drug delivery and bioimaging. In this study, we report the synthesis of gold nanoparticles (GNPs) using Piper betle leaf extract. Extraction of secondary metabolites was performed by both acetonic and ethanolic extract to establish a comparative assessment. UV-Visible spectroscopic, Transmission Electron Microscopy, Energy Dispersive X-ray and X-ray diffraction characterization confirmed the synthesis of GNPs. The results thus suggested that the metabolites of P. betle L. leaf can effectively catalyze the conversion of Au 3? to Au 0 ions and serve as excellent stabilizing agents. Morphological modulation was found with varying tetrachloroauric acid concentration and the solvent medium used. Cytotoxicity assessment of GNPs on HeLa and HEK293 signified the selective toxic behaviour of GNPs towards cancerous cell lines. Further, biocompatibility towards HEK293 cells signified that the GNPs can be used for drug delivery as well as bio-imaging applications.
Journal of Cluster Science, 2019
Synthesis of biometal nanoparticles (BM-NPs) in a green-synthetic approach is the favorite resear... more Synthesis of biometal nanoparticles (BM-NPs) in a green-synthetic approach is the favorite research in the scope nanobiotechnology. Based on the literature, numerous microorganisms have been introduced for fabrication of BM-NPs. But, to our knowledge, no comprehensive review is available to classify the species of any microorganism genera of producing BM-NPs. Hence, the present approach systematically reviewed the biogenic preparation and pharmaceutical potential of BM-NPs synthesized and stabilized by exploiting Penicillium species. Records identified through databases involving Cochrane, Web of Science, PubMed, ProQuest, Scopus, Embase, Science Direct, and Google Scholar. In this study, 238 papers were identified following the initial search of databases, whereas 179 studies were excluded and eventually 59 papers were included in the study. Remarkably, 25 Penicillium species were introduced for biosynthesis of BM-NPs. Notably, silver NPs with 70.76% of the studies were the first predominant which produced by exploiting Penicillium species. Significantly, among the studies that explored applications of NPs, most of them investigated antimicrobial efficacy of BM-NPs. Moreover, a hefty 91.22% studies reported spherical NPs, while only 8.77% refers to other morphologies. Furthermore, pharmaceutical potentials of BM-NPs were discussed in details.
Materialia, 2018
Carbides have noteworthy manifold properties, solely adjusted to the requirements of different us... more Carbides have noteworthy manifold properties, solely adjusted to the requirements of different users. Nanostructured Boron Carbide (B 4 C) is one of them which possess unique characteristics to move towards industrial wastewater treatment from its traditional uses. Present work deals with the first photocatalytic study of biocompatible nanostructured B 4 C for organic (Methylene blue, MB) as well as industrial (Synazol yellow, SY) dye. The textile industry excretes non-biodegradable waste into water bodies, so effective and economical catalyst for the removal of industrial pollutants is the need of the hour. B 4 C shows remarkable results for the degradation of MB and SY dyes. The high degradation efficiency of 91.09 % and 76.04% was achieved with 0.5 g/L catalyst for SY and MB dyes respectively loading due to the presence of defect states in the material. The effect of concentration of catalyst loading on the extent of photo-degradation has been considered for this study. As the concentration of B 4 C catalyst increases, the number of active sites on the surface of catalyst also increased which leads to improvement in degradation of dyes. Reusability of the same catalyst showed good results for its cost-effective use. The toxicity assessment revealed superior biocompatibility of B 4 C nanostructures upto the concentration ~ 800 ppm. These findings indicate
Journal of cellular biochemistry, Jan 11, 2018
N-benzhydrylpiperazine and 1,3,4-oxadiazoles are pharmacologically active scaffolds which exhibit... more N-benzhydrylpiperazine and 1,3,4-oxadiazoles are pharmacologically active scaffolds which exhibits significant inhibitory growth effects against various cancer cells, however, antiproliferation effects and the underlying mechanism for inducing apoptosis for aforementioned scaffolds addressing HeLa cancer cells remains uncertain. In this study, N-benzhydrylpiperazine clubbed with 1,3,4-oxadiazoles (4a-4h) were synthesized, subsequently characterized using high resolution spectroscopic techniques and eventually evaluated for their antiproliferation potential by inducing apoptosis in HeLa cancer cells. The MTT assay screening results revealed that among all, compound 4d ( N-benzhydryl-4-((5-(4-aminophenyl)-1,3,4-oxadiazol-2-yl)methyl)piperazine) in particular, exhibited IC value of 28.13 ± 0.21 μg/mL and significantly inhibited the proliferation of HeLa cancer cells in concentration-dependent manner. The in vitro anticancer assays for treated HeLa cells resulted in alterations in the c...
New Journal of Chemistry, 2017
Boron nitride and titanium oxide composite (BN–TiO2) photocatalyst endowed with high specific sur... more Boron nitride and titanium oxide composite (BN–TiO2) photocatalyst endowed with high specific surface area and large pore size was synthesized by ice bath method.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 11, 2017
Triple-negative breast cancers (TNBC) are aggressive cancers, which do not control by hormonal th... more Triple-negative breast cancers (TNBC) are aggressive cancers, which do not control by hormonal therapy or therapies that target HER-2 receptors. Curcumin (Cur) has shown cytotoxic effects in multiple cancer cell lines. However, its medical uses remain limited due to low aqueous solubility and poor bioavailability. Therefore, present study was aimed to fabricate the small positive charge curcumin nanoparticles (CN) by nanoprecipitation methods using PLGA and CTAB, and to evaluate its anticancer efficacy and underlying the mechanism in triple negative breast cancer cell lines (MDA-MB-231 cells). In in-vitro drug release assay, Cur was released from CN by flicking diffusion and anomalous transport process. CN showed a higher cellular incorporation than free Cur resulted in higher cytotoxicity. Checking the anticancer activity at the molecular level, Cur has shown to induce the reactive oxygen species production that subsequently causes the DNA damage and resulting in p38-MAPK activatio...
Scientific reports, Oct 19, 2016
Highly water dispersible boron based compounds are innovative and advanced materials which can be... more Highly water dispersible boron based compounds are innovative and advanced materials which can be used in Boron Neutron Capture Therapy for cancer treatment (BNCT). Present study deals with the synthesis of highly water dispersible nanostructured Boron Nitride (BN). Unique and relatively low temperature synthesis route is the soul of present study. The morphological examinations (Scanning/transmission electron microscopy) of synthesized nanostructures showed that they are in transient phase from two dimensional hexagonal sheets to nanotubes. It is also supported by dual energy band gap of these materials calculated from UV- visible spectrum of the material. The theoretically calculated band gap also supports the same (calculated by virtual nano lab Software). X-ray diffraction (XRD) analysis shows that the synthesized material has deformed structure which is further supported by Raman spectroscopy. The structural aspect of high water disperse ability of BN is also studied. The ultra...
Journal of agricultural and food chemistry, Jan 7, 2016
Normal tissue damage from ionizing radiation during radiotherapy is a major concern in cancer tre... more Normal tissue damage from ionizing radiation during radiotherapy is a major concern in cancer treatment. Tea polyphenols (TPs) have shown to reduce radiation-induced damage in multiple studies, but its pharmacological application is still limited due to poor bioavailability. Present study was aimed to increase TPs bioavailability by nanoformulation using BSA as matrix and chitosan as external shell. Encapsulated TPs nanoparticles were spherical in size and promoted TPs stability in normal and gastrointestinal condition without losing antioxidant activity. Oral administration of nanoparticles for 3 days prior to irradiation exposure has shown to protect mice from haematological injuries that result in reduction of radiation-induced lethality. TPs reduces radiation-induced oxidative damage and apoptosis by restoring the redox status through Nrf2-ERK pathway and reducing Bax expression respectively. Regarding potency, encapsulated TPs have shown a significantly higher level of radiopro...
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Papers by ramovatar meena