Papers by Pankajkumar Waghmare
Chinese Journal of Biotechnology, Jun 25, 2022
Journal of Microbiology and Biotechnology, 2021
Efficient cellulolytic enzyme production is important for the development of lignocellulosedegrad... more Efficient cellulolytic enzyme production is important for the development of lignocellulosedegrading enzyme mixtures. However, purification of cellulases from their native hosts is time-and labor-consuming. In this study, a constitutive expression system was developed in Penicillium oxalicum for the secreted production of proteins. Using a constitutive polyubiquitin gene promoter and cultivating with glucose as the sole carbon source, nine cellulolytic enzymes of different origins with relatively high purity were produced within 48 h. When supplemented to a commercial cellulase preparation, cellobiohydrolase I from P. funiculosum and cellobiohydrolase II from Talaromyces verruculosus showed remarkable enhancing effects on the hydrolysis of steam-exploded corn stover. Additionally, a synergistic effect was observed for these two cellobiohydrolases during the hydrolysis. Taken together, the constitutive expression system provides a convenient tool for the production of cellulolytic enzymes, which is expected to be useful in the development of highly efficient lignocellulose-degrading enzyme mixtures.
Applied Biochemistry and Biotechnology, 2020
We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulo... more We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars. Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp. KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes. Water hyacinth produced (U ml −1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml −1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp. KNU. Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids. The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml −1 and exoglucanase; 12.20 U ml −1), and by T. viride (filter paperase; 1.57 U ml −1). Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml −1). The highest xylanase (1129 U ml −1) was observed in T. viride + P. chrysosporium co-culture. This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.
Biofuel Research Journal, 2018
HIGHLIGHTS Biological pretreatment of sorghum husk using Phanerochaete chrysosporium was investi... more HIGHLIGHTS Biological pretreatment of sorghum husk using Phanerochaete chrysosporium was investigated. Activities of different ligninolytic and hydrolytic enzymes during biological pretreatment were evaluated. Significant increase in reducing sugar production as a result of biological pretreatment of sorghum husk vs. untreated biomass. Biological pretreatment led to substantial surface morphological changes.
3 Biotech, 2018
In this study, we have described three steps to produce ethanol from Pogonatherum crinitum, which... more In this study, we have described three steps to produce ethanol from Pogonatherum crinitum, which was derived after the treatment of textile wastewater. (a) Production of biomass: biomass samples collected from a hydroponic P. crinitum phytoreactor treating dye textile effluents and augmented with Ca-alginate immobilized growth-promoting bacterium, Bacillus pumilus strain PgJ (consortium phytoreactor), and waste sorghum husks were collected and dried. Compositional analysis of biomass (consortium phytoreactor) showed that the concentration of cellulose, hemicelluloses and lignin was 42, 30 and 17%, respectively, whereas the biomass samples without the growth-promoting bacterium (normal phytoreactor) was slightly lower, 40, 29 and 16%, respectively. (b) Hydrolysate (sugar) production: a crude sample of the fungus, Phanerochaete chrysosporium containing hydrolytic enzymes such as endoglucanase (53.25 U/ml), exoglucanase (8.38 U/ml), glucoamylase (115.04 U/ ml), xylanase (83.88 U/ml), LiP (0.972 U/ml) and MnP (0.459 U/ml) was obtained, and added to consortium, normal and control phytoreactor derived biomass supplemented with Tween-20 (0.2% v/v). The hydrolysate of biomass from consortium phytoreactor produced maximum reducing sugar (0.93 g/l) than hydrolysates of normal phytoreactor biomass (0.82 g/l) and control phytoreactor biomass (0.79 g/l). FTIR and XRD analysis confirmed structural changes in treated biomass. (c) Ethanol production: the bioethanol produced from enzymatic hydrolysates of waste biomass of consortium and normal phytoreactor using Saccharomyces cerevisiae (KCTC 7296) was 42.2 and 39.4 g/l, respectively, while control phytoreactor biomass hydrolysate showed only 25.5 g/l. Thus, the amalgamation of phytoremediation and bioethanol production can be the truly environment-friendly way to eliminate the problem of textile dye along with bioenergy generation.
Agriculture and Natural Resources, 2018
A cellulolytic bacterium was isolated from plant litter soil and identified as Enterobacter sp. S... more A cellulolytic bacterium was isolated from plant litter soil and identified as Enterobacter sp. SUK-Bio. This isolate was investigated for its utilization of different cellulosic materials (carboxymethyl cellulose, sugarcane trash, grass powder, sorghum husk, wheat straw and water hyacinth). Utilization of sorghum husk was comparatively more than for the other cellulosic materials used, producing higher cellulolytic and hemicellulolytic enzymes (filter paperase (0.15 U/mL), b-glucosidase (37.10 U/mL), endoglucanase (12.24 U/mL), exoglucanase (2.52 U/mL), xylanase (26.26 U/mL) and glucoamylase (33.26 U/mL)) on day 8 of incubation. Furthermore, it produced the maximum reducing sugar production (554 mg/L) at a rate of 3.84 mg/h/L. Fourier-transform infrared spectroscopy analysis of sorghum husk revealed functional groups changes and a decrease in the total crystallinity ratio after microbial degradation. The effects of supplementation of different metals additives, thermal stability and pH on cellulolytic enzymes were also studied.
Gene Reports, 2017
Aspergillus cellulase was observed to show superior hydrolysis of microcrystalline cellulose (Avi... more Aspergillus cellulase was observed to show superior hydrolysis of microcrystalline cellulose (Avicel PH101) when compared to Trichoderma cellulase. In silico physicochemical characterization revealed the acidic nature of selected fungal cellulases. GRAVY index for Aspergillus and Trichoderma cellulase was-0.314 and-0.374, respectively. Negative values of GRAVY index for both the fungal cellulases indicate that these enzymes have better interaction with water. Secondary structures such as alpha helices (34.44%) and random coils (54.21%) were found to be dominant in A. niger and T. longibrachiatum cellulase, respectively. Validation results for homology modeled 3D structures of selected cellulases indicated their fine quality. The analyses revealed that 96.7 and 92.4 % residues of the constructed cellulase models of A. niger and T. longibrachiatum were placed in the favored region, respectively with >82 ERRAT overall quality factor and have more than 0.7 QMEAN 4 score. ProSA evaluation revealed that the Z-score for A. niger and T. longibrachiatum cellulase was-9.2 and-6.78, respectively. Energy minimization results revealed that the potential energies-7.140 X 10 5 kJ/mol and-1.228 X 10 6 kJ/mol were found remaining constant after 744 and 1431 steps for A. niger and T. longibrachiatum cellulases, respectively. Molecular docking study revealed that TRP 200, THR 201 and GLU 160 residues from A. niger cellulase and ASP 220, GLN 196, TYR 192 and TYR 168 residues from T. longibrachiatum cellulase interact with the cellulose. Studies such as this could prove to be helpful in terms of formulating cellulase enzyme for the purpose of industrial use.
Journal of Materials Science: Materials in Electronics, 2015
ABSTRACT In this article, ZnO nanostructures with diverse morphologies have been synthesized via ... more ABSTRACT In this article, ZnO nanostructures with diverse morphologies have been synthesized via a simple, rapid and cost effective solid state thermal decomposition method. The as-synthesized ZnO nanostructures were characterized by means of X-ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscope (SEM), high-resolution transmission electron microscope (HR-TEM), UV–Vis. diffuse reflectance spectra (UV–Vis-DRS) and photoluminescence (PL). The XRD patterns and HR-TEM image indicated a hexagonal wurtzite structure of ZnO. The SEM images of ZnO samples show the different morphologies such as nanowire, nanorods, spherical and irregular microsphere forat different calcination temperatures. Room temperature PL spectra of the samples exhibited characteristics blue and green emission bands in accordance with calcination temperature. Moreover, photocatalytic as well as antimicrobial activities were evaluated using ZnO synthesized at different calcination temperatures. The effects of calcination temperature, catalyst loading and pH on the photodegradation efficiency were systematically studied. A highest (99 %) photocatalytic activity of ZnO nanorods towards the Acid Green 25 (AG-25) was achieved within 35 min at optimal conditions under UV light. As-synthesized ZnO nanorods are found to be more efficient than TiO2 (P25) towards the degradation of AG-25. It was found that the antimicrobial activity of ZnO nanorods (13 mm) obtained at 300 °C showed significantly higher inhibition efficiencies than the other samples. The mineralization of AG-25 was confirmed from a reduction of 85 % the chemical oxygen demand within 35 min. In addition, ZnO nanorods could be easily reusable up to four runs without changing its photocatalytic activity.
RSC Advances, 2015
Efficient conversion of fermentable sugars from cheap lignocellulosic biomass is a current need i... more Efficient conversion of fermentable sugars from cheap lignocellulosic biomass is a current need in viable ethanol production technology.
Journal of Hazardous Materials, 2015
A static hydroponic bioreactor using nursery grown plants of Pogonatherum crinitum along with imm... more A static hydroponic bioreactor using nursery grown plants of Pogonatherum crinitum along with immobilized Bacillus pumilus cells was developed for the treatment of textile wastewater. Independent reactors with plants and immobilized cells were also kept for performance and efficacy evaluation. The effluent samples characterized before and after their treatment showed that the plant-bacterial consortium reactor was found to be more efficient than those of individual plant and bacterium reactors. COD, BOD, ADMI, conductivity, turbidity, TDS and TSS of the textile effluent was found to be reduced by 78, 70, 93, 4, 90, 13 and 70 % respectively within 12 d by the consortial set. HPTLC analysis revealed the transformation of the textile effluent to new products. The phytotoxicity study on Phaeseolus mungo and Sorghum vulgare seeds showed reduced toxicity of treated effluents. The animal toxicity study performed on Etheostoma olmstedi fishes showed the toxic nature of untreated effluent giving extreme stress to fishes leading to death. Histology of fish gills exposed to treated effluent was found to be less affected. The oxidative stress related enzymes like superoxide dismutase and catalase were found to show decreased activities and less lipid peroxidation in fishes exposed to treated effluent.
Bioresource Technology, 2014
h i g h l i g h t s Adsoption of textile dye RB172 on agricultural waste SCB biomass. Biodegradat... more h i g h l i g h t s Adsoption of textile dye RB172 on agricultural waste SCB biomass. Biodegradation of SCB adsorbed RB172 under solid state fermentation by P. stuarti. Production of cellulolytic crude enzyme from Phanerochaete chrysosporium NCIM 1106. Enzymatic hydrolysis of waste biomass (ddSCB) obtained after bioremediation of RB172. HPTLC, FTIR, XRD and SEM characterization of SCB and ddSCB after enzymatic hydrolysis.
3 Biotech, 2017
Nocardiopsis sp. KNU was found to degrade various lignocellulosic waste materials, namely, sorghu... more Nocardiopsis sp. KNU was found to degrade various lignocellulosic waste materials, namely, sorghum husk, sugarcane tops and leaves, wheat straw, and rice husk very efficiently. The strain was found to produce high amounts of cellulase and hemicellulase. Augmentation of cotton seed cake as an organic nitrogen source revealed inductions in activities of endoglucanase, glucoamylase, and xylanase up to 70.03, 447.89, and 275.10 U/ml, respectively. Nonionic surfactant Tween-80 addition was found to enhance the activity of endoglucanase enzyme. Cellulase produced by Nocardiopsis sp. KNU utilizing sorghum husk as a substrate was found to retain its stability in various surfactants up to 90%. The produced enzyme was further tested for saccharification of mild alkali pretreated rice husk. The changes in morphology and functional group were analyzed using scanning electron microscopy and Fourier transform infrared spectroscopy. Enzymatic saccharification confirmed the hydrolytic potential of ...
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Papers by Pankajkumar Waghmare