Papers by Masood Naseripour
Von Hippel-Lindau (VHL) syndrome is an autosomal dominant inherited multisystem neoplasia disorde... more Von Hippel-Lindau (VHL) syndrome is an autosomal dominant inherited multisystem neoplasia disorder caused by the VHL tumor suppressor gene, coding for VHL protein (pVHL), variants. Various types of VHLvariants present different clinical phenotypes that later lead to events resulting in benign or malignant lesions including Retinal Capillary Hemangioblastoma (RCH). In this case series study, 3 families (5 RCH patients) with novel mutations were included. VHL mutational analysis was performed by Sanger sequencing. We reported on 3 novel mutation sites, including c.511A>C, c.514C>T, and c.511A>T in exon 3 of the VHL gene. According to the ACMG classifications, c.514C>T and c.511A>T variations are likely pathogenic, and c.511A>C is a variant of uncertain significance (VUS) and in accordance with autosomal dominant inheritance. The location and impact of the incidence mutations on pVHL were computed using in silico analysis. The obtained structural information and compu...
Journal of Current Ophthalmology
Journal of Biomaterials Science, Polymer Edition
BMC Ophthalmology
Background To report the long-term outcomes of Ru-106 plaque radiotherapy in eyes with uveal mela... more Background To report the long-term outcomes of Ru-106 plaque radiotherapy in eyes with uveal melanoma (UM) and to assess the effect of tumor thickness and location on final outcomes. Methods Medical records of 234 patients undergoing Ru-106 plaque radiotherapy for UM were reviewed, and the visual outcome, globe preservation, and patient survival were evaluated. The results of 2 groups were compared: 1. between thin (small and medium-sized, thickness < 7 mm, 148 eyes [63.2%]) and thick (thickness ≥ 7 mm, 86 eyes [36.8%]) tumors, and 2. between large (largest basal diameter [LBD] > 12 mm, 109 eyes [46.6%]) and medium/small (LBD ≤ 12 mm, 125 eyes [53.4%]). In addition, a comparison of the juxtapapillary location in 46 eyes (19.7%) versus tumors arising elsewhere and between tumors with and without ciliary involvement in 48 eyes (21.5%) were done. Results The patients were followed for a median of 54.2 months (range: 6–194.5 months). After adjusting for baseline visual acuity (VA)...
To study the pattern of perfusion of central retinal artery (CRA) after 0.1 ml intravitreal injec... more To study the pattern of perfusion of central retinal artery (CRA) after 0.1 ml intravitreal injection of bevacizumab to verify the need for any maneuver to decrease the intraocular pressure (IOP) including anterior chamber paracentesis (ACP) Methods: This is a prospective, interventional, noncomparative case series. Patients receiving intravitreal injection of bevacizumab for choroidal neovascularization (CNV) secondary to agerelated macular degeneration, diabetic macular edema and retinal veno-occlusive diseases were included in the study. Each eye received 0.1 ml intravitreal injection of bevacizumab and the status of perfusion of CRA and its pulsation was monitored by indirect ophthalmoscopy until cessation of visible pulsation. Main outcome measures were patency of CRA, its pulsation and time from injection to cessation of pulsation. Results: Seventy seven eyes of 70 patients were studied. At first ophthalmoscopy 30 seconds after injection, CRA was open in all cases with or without pulsation. CRA occlusion was not observed in any case. In 20 eyes (26%) CRA was patent without pulsation. In 57 eyes (74%) CRA pulsation was detected and this visible pulsation of CRA stopped within an average time of 167±99 seconds (range: 30-480 seconds). From 17 eyes which had significant vitreous reflux, only 6 eyes had CRA pulsation which stopped in a mean time of 80±36 seconds. There was a significant difference between pulsation duration in patients with and without vitreous reflux (Mann-Whitney U test, P=0.005). Absence of postinjection vitreous reflux was a risk factor for CRA pulsation after intravitreal injection of 0.1 ml of bevacizumab (relative risk: 2.41, 95% CI: 1.25-4.62). Conclusion: Considering the absence of CRA closure and the short time needed for the cessation of pulsation after intravitreal injection of 0.1 ml bevacizumab, no treatment including ACP is warranted before or after such injections in nonglaucomatous eyes. Indirect ophthalmoscopy is a noninvasive useful maneuver to ascertain patency of CRA after intravitreal injections.
European Journal of Ophthalmology
Background/ Objectives Despite being the most common intraocular malignancy in childhood, there i... more Background/ Objectives Despite being the most common intraocular malignancy in childhood, there is a substantial disparity between developing and developed countries in terms of patient and globe survival in retinoblastoma (Rb). The current study intends to determine patient and globe survival before and after the introduction of the new targeted treatment modalities in a developing country. Methods Medical records of 350 patients (516 eyes) with retinoblastoma referred to a tertiary referral center for Rb in Tehran, Iran, were reviewed. In order to compare patient and globe survival before and after the availability of the new treatment modalities, including intra-arterial and intravitreal chemotherapy, the patients were divided into group 1 (2001–2007) and group 2 (2008–2018) based on the calendar period of diagnosis. Results Two-hundred-twenty-three eyes of 149 patients and 293 eyes of 201 patients were categorized into groups 1 and 2, respectively. The 5-year patient survival wa...
Stem Cell Research & Therapy
Retinal pigment epithelium (RPE) degeneration is the hallmark of age-related macular degeneration... more Retinal pigment epithelium (RPE) degeneration is the hallmark of age-related macular degeneration (AMD). AMD, as one of the most common causes of irreversible visual impairment worldwide, remains in need of an appropriate approach to restore retinal function. Wet AMD, which is characterized by neovascular formation, can be stabilized by currently available therapies, including laser photocoagulation, photodynamic therapy, and intraocular injections of anti-VEFG (anti-vascular endothelial growth factor) therapy or a combination of these modalities. Unlike wet AMD, there is no effective therapy for progressive dry (non-neovascular) AMD. However, stem cell-based therapies, a part of regenerative medicine, have shown promising results for retinal degenerative diseases such as AMD. The goal of RPE cell therapy is to return the normal structure and function of the retina by re-establishing its interaction with photoreceptors, which is essential to vision. Considering the limited source of...
Backgrounds: Retinal capillary hemangioblastoma (RCH) although may also be observed sporadically,... more Backgrounds: Retinal capillary hemangioblastoma (RCH) although may also be observed sporadically, is the most frequent and earliest manifestation of the Von Hippel-Lindau disease (VHL). This is the first report of different types of variants spectrum and genotype-phenotype correlations in the Iranian families with RCH. Methods: In this Prospective Observational Case Series study, 17 families with RCH were included. PCR was performed to amplify 3 exons of the VHL gene. Afterwards, Sanger sequencing was performed on all the PCR products. For the detection of VHL copy number variations, MLPA was used. Results: Our study identified 10 different types of predominantly pathogenic VHL variants. Missense mutations were the most common variants located on the α domain of the VHL protein (pVHL) in exon 3. Also, the pathogenic variants in RCH patients with CNS hemangioblastoma (CHB) mostly (72.7%) were located on the α domain. In a family, Proband’s sister had a different mutation with more se...
Eye, 2020
Background/objectives Early diagnosis, care and treatment of retinoblastoma is a challengeable is... more Background/objectives Early diagnosis, care and treatment of retinoblastoma is a challengeable issue for Iranian health system. This study was designed and conducted in a referral multidisciplinary centre in the capital city of Iran to evaluate management, care, prognosis and survival rates of paediatric patients with retinoblastoma. Methods In this retrospective study, a total number of 309 patients younger than 15 years, diagnosed with retinoblastoma, who referred for diagnosis and treatment to MAHAK's Pediatric Cancer Treatment and Research Center (MPCTRC) from 2007 to 2017 were evaluated. All data were analyzed via SPSS version 22 software in regard of parametric and nonparametric data. Survival rates were analyzed using the Kaplan-Meyer method. Results The mean age of patients was 20 months and the majority of patients (77%) had leukocoria as a common clinical symptom at the time of diagnosis. Primary treatment methods were systemic chemotherapy (94%), laser (35%) and primary enucleation (28%). Relapses occurred in nearly 42% of cases, and the median time from diagnosis to the first relapse was 9 months. At the time analyzing the data, 11% of patients died. Patients' 5-year OS and RFS rates were 79.6% and 41.5%, respectively. Conclusion Comparing results with other conducted studies identifies that the recurrence rate was high in our considered patients. Also, OS and RFS rates in our study were not as considerable as other reports. Screening methods, updating protocols and follow-up of patients may lead to improvements in survival rates of patients with retinoblastoma.
Journal of Current Ophthalmology, 2018
Purpose: To present a rare anaplastic form of retinal pleomorphic xanthoastrocytoma (PXA) unassoc... more Purpose: To present a rare anaplastic form of retinal pleomorphic xanthoastrocytoma (PXA) unassociated with phakomatosis. Methods: A 9-year-old girl, presented with a rapidly growing unilateral intraocular white mass unresponsive to intra-arterial chemotherapy, underwent enucleation with the clinical suspicion of retinoblastoma versus malignant astrocytoma. Results: Histopathology revealed pleomorphic cells with rosenthal fibers, mitosis, and necrosis. Immunohistochemistry confirmed the diagnosis of anaplastic pleomorphic xanthoastrocytoma (aPXA). The patient had no signs of phakomatosis. Conclusion: Retinal PXA may occur in patients without phakomatosis and rarely progress toward malignant transformation.
Iranian Journal of Ophthalmology. سامانه جامع مدیریت اطلاعات پژوهشی. http://irjo.jo.research.ac.i... more Iranian Journal of Ophthalmology. سامانه جامع مدیریت اطلاعات پژوهشی. http://irjo.jo.research.ac.ir. ...
Journal of contemporary brachytherapy, 2016
To evaluate the outcomes of ruthenium-106 ((106)Ru) brachytherapy in terms of radiation parameter... more To evaluate the outcomes of ruthenium-106 ((106)Ru) brachytherapy in terms of radiation parameters in patients with thick uveal melanomas. Medical records of 51 patients with thick (thickness ≥ 7 mm and < 11 mm) uveal melanoma treated with (106)Ru brachytherapy during a ten-year period were reviewed. Radiation parameters, tumor regression, best corrected visual acuity (BCVA), and treatment-related complications were assessed. Fifty one eyes of 51 consecutive patients including 25 men and 26 women with a mean age of 50.5 ± 15.2 years were enrolled. Patients were followed for 36.1 ± 26.5 months (mean ± SD). Mean radiation dose to tumor apex and to sclera were 71 (± 19.2) Gy and 1269 (± 168.2) Gy. Radiation dose rates to tumor apex and to sclera were 0.37 (± 0.14) Gy/h and 6.44 (± 1.50) Gy/h. Globe preservation was achieved in 82.4%. Preoperative mean tumor thickness of 8.1 (± 0.9) mm decreased to 4.5 (± 1.6) mm, 3.4 (± 1.4) mm, and 3.0 (± 1.46) mm at 12, 24, and 48 months after bra...
Indian Journal of Ophthalmology, 2021
Purpose: Plaque therapy is a well-recognized treatment for intraocular tumors. In current study, ... more Purpose: Plaque therapy is a well-recognized treatment for intraocular tumors. In current study, we aimed to prospectively investigate the short-term effects of ruthenium 106 (Ru-106) plaque therapy on the cornea and ocular surface parameters. Methods: Twenty-five patients diagnosed with choroidal melanoma which undergone Ru-106 plaque therapy from 2016 to 2018 were included. Tear osmolarity, tear film break-up time, Schirmer test I, fluorescein dye staining based on Oxford staining method; Ocular Surface Disease Index (OSDI) questionnaire and corneal specular microscopy were performed. These tests were assessed preoperatively and then 3 months postoperatively. Results: The mean (±SD) age of subjects was 48.52 ± 15.18 years. The patients were followed for a mean(±SD) period of 3.64 ± 2.40 months. Total mean (+SD) delivered radiation dose to the tumor apex and total received radiation by the sclera was 83.20 ± 26.31 and 640.65 ± 472.69 Gray (Gy), respectively. In longitudinal analysi...
Purpose: To report a rare case of large vascularized astrocytoma on optic disc. Case Report: A 12... more Purpose: To report a rare case of large vascularized astrocytoma on optic disc. Case Report: A 12-year-old boy with history of decreased vision in the left eye since 5-6 months ago was referred to the ocular oncology service at Rassoul Akram Hospital with provisional diagnosis of retinoblastoma. On examination, visual acuity was counting fingers at 50 cm, and a 3+ relative afferent pupillary defect response as well as an exodeviation was evident in the left eye. Fundus examination of the left eye revealed a large yellow-white mass with superficial vessels overhanging the optic disc. Right eye slit lamp exam and funduscopy were unremarkable. No clinical manifestation of tuberous sclerosis was found. Orbital CT scan, MRI and ultrasonography of the globe was done. Enucleation was performed because of the development of iris neovascularization and progressive enlargement of the tumor. Histologic exam confirmed the diagnosis of astrocytoma.
Systemic chemotherapy regimens and focal treatment modalities such as radioactive plaque therapy ... more Systemic chemotherapy regimens and focal treatment modalities such as radioactive plaque therapy have dramatically improved the outcomes of treatment for retinoblastoma such that patient survival with modern monitoring and treatment now exceeds 95%. Although the vast majority of patients respond adequately to current therapeutic strategies, treatment for a subset of patients with advanced retinoblastoma remains challenging. Consider an infant with advanced bilateral retinoblastoma, group D international classification (diffuse vitreous and subretinal seedings of the tumor) in one eye and group E (anterior segment invasion of the tumor) in the fellow eye; treatment options for such a patient are limited to repetitive courses of systemic and periocular chemotherapy, and/or external beam radiation therapy. These modalities are usually associated with significant adverse effects, including bone marrow suppression, hearing loss, systemic infections and increased risk of secondary cancers...
Eye
Optical coherence tomography angiography (OCTA) is a revolutionary method in the visualization of... more Optical coherence tomography angiography (OCTA) is a revolutionary method in the visualization of the vascular system in different retinal and choroidal layers. During the last 4 years since the commercial availability of different OCTA devices, attempts have been made to utilize this technology in various aspects of ocular oncology from the differentiation of benign and malignant lesions to assisting in evaluation of post-treatment complications, such as radiation retinopathy. However, current OCTA technology is restricted by various artefacts and inherent limitations, some of which are more pronounced in the presence of elevated tumoural lesions. Imminent advancements in OCTA systems and image acquisition processes promise a great potential for application of OCTA in ocular oncology.
Current Drug Therapy
Objective: The present study was designed to evaluate the effects of Atorvastatin (ATO) plus Mela... more Objective: The present study was designed to evaluate the effects of Atorvastatin (ATO) plus Melatonin (MEL) on streptozocin-induced Diabetic Retinopathy (DR) in rats. Methods: Diabetes was induced in Wistar rats with an intraperitoneal injection of streptozocin (50 mg/kg). Animals were randomly assigned to one of the following groups (8 rats/group): Control group, Diabetic group, Diabetic + MEL group (20 mg/kg/day), Diabetic + ATO group (10 mg/kg/day), Diabetic + MEL + ATO group (as above). Treatments were started one week after induction of diabetes and continued for 7 weeks. At the end of the experiment, angiography was performed and the rats were killed and retinas were harvested for pathological and molecular examinations. Results: Administration of MEL reduced the fluorescein leakage, MDA and ROS levels compared to diabetic group. Treatment with ATO only reduced ROS levels compared to diabetic group. In addition, administration of ATO plus MEL decreased these indices compared ...
Current Pharmaceutical Design
Optic neuropathies refer to the dysfunction or degeneration of optic nerve fibers caused by any r... more Optic neuropathies refer to the dysfunction or degeneration of optic nerve fibers caused by any reasons including ischemia, inflammation, trauma, tumor, mitochondrial dysfunction, toxins, nutritional deficiency, inheritance, etc. Post-mitotic CNS neurons, including retinal ganglion cells (RGCs) intrinsically have a limited capacity for axon growth after either trauma or disease, leading to irreversible vision loss. In recent years, an increasing number of laboratory evidence has evaluated optic nerve injuries, focusing on molecular signaling pathways involved in RGC death. Trophic factor deprivation (TFD), inflammation, oxidative stress, mitochondrial dysfunction, glutamate-induced excitotoxicity, ischemia, hypoxia, etc. have been recognized as important molecular mechanisms leading to RGC apoptosis. Understanding these obstacles provides a better view to find out new strategies against retinal cell damage. Melatonin, as a wide-spectrum antioxidant and powerful freeradical scavenger...
VirusDisease
Retinoblastoma tumor (RB) is one of the most prevalent ocular cancers among children. RB may be c... more Retinoblastoma tumor (RB) is one of the most prevalent ocular cancers among children. RB may be caused by inherited mutations in RB1 gene as well as some environmental risk factors. Human papillomaviruses (HPV) are suspected as a risk factor of RB due to their pRb inactivating protein. This study evaluated the molecular prevalence of HPV among the RB tumor specimens in Iran. The RB tumor samples were tested for detection of HPV-L1 gene using a nested-PCR approach, and then followed by sequencing and phylogenetic analysis to reveal HPV types. Overall, there were 61 RB tumor samples; 54/61 (88.5%) had unilateral and 7/61 (11.5%) bilateral RB; 55/61 cases (90.2%) had sporadic non-familial RB tumor. HPV-DNA was detected in 6/61 (9.8%) of patients’ tumors; the HPV positive RB cases all had unilateral and unfamiliar sporadic RB tumor. HPV type 16 was the most prevalent type identified across the RB tumor samples (3/61, 4.9%). The rate of detected HPV among the RB specimens seems to be considerable. Further investigations are required to elucidate the exact association between HPV and progression to RB.
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Papers by Masood Naseripour