Papers by maryani cyccu tobing
Deleted Journal, May 22, 2024
Fall armyworm (Spodoptera frugiperda) is one of the pests that attacks maize. There are many ways... more Fall armyworm (Spodoptera frugiperda) is one of the pests that attacks maize. There are many ways to control this pest problem one of which is to use insecticides. However, biological control this pest with nature conservation based on the concept of integrated pest control. Refugia plants offer pest control properties based on the IPM (Integrated Pest Management) concept by providing food for parasitoids as the natural enemies. This study aimed to know capacity parasitism of egg parasitoid S. frugiperda in refugia system. This research uses non-factorial randomized group design (RGD) study, the number of S. frugiperda egg parasitoids on maize fields with refugia plants around it was counted. There were 5 treatments with 5 replications applied in this study: Control/without refugia, maize plants with red refugia (Zinnia elegans), maize plants with yellow refugia (Melanpodium paludosumi), maize plants with white refugia (Turnera subulata), maize plants with red refugia, yellow and white. This study found two species of S. frugiperda egg parasitoids namely Telenomus remus Nixon (Hymenoptera: Scelionidae) and Telenomus dignus (Hymenoptera: Scelionidae) in the field. The overall rate of parasitism egg S. frugiperda was no significant effect. The treatment of maize plants with white and mixed-colour refugia (red, yellow, white) showed the highest egg parasitization at 15 days after planting (DAP). Meanwhile, the dominance of Telenomus remus Nixon parasitoid was at an average of 0.202% and the lowest dominance of Telenomus dignus at 0.030%.
Jurnal Agroteknologi, Jun 15, 2023
Species of parasitoids on maize is the beginning to control S. frugiperda pests. An Integrated Pe... more Species of parasitoids on maize is the beginning to control S. frugiperda pests. An Integrated Pest Management (IPM) strategy is needed to reduce the population of S. frugiperda. This research was carried out on land owned by farmers in Purwobinangun Village, Sei Bingai District, Langkat Regency (February to June 2021). The research used a Factorial Randomized Block Design with 2 factors are cropping patterns (Monoculture and Intercropping) and varieties (Bisi 18 and Pioneer 32) with six replications. The results showed that 2 genera egg parasitoids of S. frugiperda are Telenomus and Trichogramma. The highest parasitization (28,32%) was in the intercropping treatment of maize variety Pioneer 32 (P1V0) and the lowest parasitization (12,47%) was in the monoculture treatment of maize variety Bisi 18 (P0V1). The egg parasitoid of S. frugiperda was dominated by the Telenomus.
IOP conference series, Jun 1, 2022
The authors conducted a survey of Pineapple Mealybug Wilt-associated Virus (PMWaV) on pineapple p... more The authors conducted a survey of Pineapple Mealybug Wilt-associated Virus (PMWaV) on pineapple plants in four pineapple-producing sub-districts in North Tapanuli, namely Sipahutar, Pangaribuan, Siborongborong, and Tarutung. This survey aims to detect the presence and distribution of PMWaV in North Tapanuli. The survey found PMWaV in pineapple plants in all survey locations with varying symptoms such as wilting to a yellow-red colour and curling leaf tips, curling and drying leaves and leaf tips, and showing symptoms of wilting to a redand yellow colour. The results showed an incidence rate between 15.6 to 80%. Sampang ni Huta V, Sipahutar, showed the lowest incidence rate (15.6%), while Lobu Siregar, Siborongborong, showed the highest (80.0%). Meanwhile, the severity rate was between 14.0 and 65.0%. Pancur Natolu, Pangaribuan, showed the highest severity rate (65.0%, severe), while Siabal-abal II, Sipahutar, showed the lowest (14.0%, moderate).
IOP conference series, Jun 1, 2021
Pineapple Mealybug Wilt-associated Virus (PMWaV) is one of the main diseases that affect pineappl... more Pineapple Mealybug Wilt-associated Virus (PMWaV) is one of the main diseases that affect pineapples all over the world, including Sipahutar, North Tapanuli, Indonesia. Currently, there are no studies or information on PMWaV disease as well as the species of mealybugs found in sipahutar pineapples. Therefore, this study aims to identify the mealybugs causing PMWaV in sipahutar pineapple. Data were obtained from 3 (three) villages, namely Siabal-abal II, Onan Runggu, and Sabunganni Huta V, to assess the disease incidence and collect mealybugs. The results showed that the disease incidence of PMWaV in the 3 villages were between 15.6 - 21.6%. The highest disease incidence was found in Siabal-abal II (21.6%), followed by Onan Runggu (21.3%), and SabunganniHuta V (15.6%). The mealybugs found on pineapple sipahutar with PMWaV symptoms in the 3 surveyed villages were identified as Dysmicoccus brevipes.
IOP conference series, Jun 1, 2021
Fungi are heterotrophic organisms that can find in many habitat likes soil rhizosphere, and insec... more Fungi are heterotrophic organisms that can find in many habitat likes soil rhizosphere, and insect larvae in the field. The purpose of this study was to determine the diversity of fungi in the soil rhizosphere of corn and Spodeptera frugiperda larvae that are attacked by fungi in the field. Sampling of soil and insects infested by fungi in this study was carried out in a corn cropping in Gedung Johor Sub-district, Medan Johor District, North Sumatra Province, Indonesia. Isolation and identification were conducted at the Biology Laboratory, Universitas Negeri Medan. The results showed that the number of fungi found from the soil rhizosphere of corn plants was higher than from S. frugiperda larvae that were attacked by the fungus. There were 16 fungal isolates found from the soil rhizosphere of corn, namely: Penicillium (1 isolate), Trichoderma (2 isolates), Fusarium (3 isolates), Mucor (1 isolate), Aspergillus (3 isolates), and 6 isolates have not identified yet. Meanwhile, 9 fungal isolates found from S. frugiperda, consisting of 6 isolates from Aspergillus, and 3 other isolates have not been identified.
Journal of physics, Mar 1, 2020
This study aims to compare the effectiveness of two attractants and three different heights trap ... more This study aims to compare the effectiveness of two attractants and three different heights trap to trap coffee berry borer Hypothenemus hampei Ferr. (Coleoptera: Scolytidae). This research was conducted at a coffee plantation in Soban Village, Dairi Regency, North Sumatra, using a Factorial Randomized Block Design (RBD) with two factors. The first factor was the type of attractants (control, coffee bean, and coffee bean pod (exocarp)), and the second factor was the height of the traps (1, 1.5, and 2 meters). The results showed that the attractant from coffee beans with a trap height of 1.5 meters gave the best results with the number of adult coffee bean borer trap catches was 1,377.00 adults, and significantly reduced the percentage of attack by 26.95% compared to other treatments.
Jurnal Entomologi Indonesia, Jun 8, 2023
ABSTRAK Metarizhium anisopliae (Metsch.) adalah cendawan entomopatogen yang umum menyerang kumban... more ABSTRAK Metarizhium anisopliae (Metsch.) adalah cendawan entomopatogen yang umum menyerang kumbang Oryctes rhinoceros (Linnaeus). Cendawan ini adalah salah satu yang berpotensi dalam mewujudkan pengelolaan hama berkelanjutan karena ramah terhadap lingkungan. Tujuan penelitian ini adalah mengisolasi cendawan M. anisopliae dari berbagai sampel tanah pertanaman kelapa sawit dan melihat keragaman genetiknya berdasarkan penanda random amplification of polymorphic DNA (RAPD). Metode yang dilakukan dalam penelitian ini adalah identifikasi M. anisopliae secara morfologi dan molekuler (ITS), dan pengujian keragaman genetik menggunakan penanda RAPD. Lima belas isolat M. anisopliae berasal dari lima belas perkebunan rakyat kelapa sawit di Sumatera Utara diuji keragaman genetiknya. Berdasarkan karakter morfologinya semua isolat diidentifikasi sebagai Metarhizium sp. Hasil sekuens dengan primer ITS menunjukkan semua isolat M. anisopliae yang yang berasal dari tanah memiliki homologi 85-99% dengan strains M. anisopliae dan M. brunneum rujukan dari database bank gen National Center Biotechnology Information (NCBI). Delapan primer RAPD menghasilkan 39 pita yang mana 38 pita (98,44%) dari pita tersebut polimorfik. Kisaran jarak genetik atau ketidaksamaan genetik antara isolat M. anisopliae adalah 0,051 sampai 0,520%. Analisis pengelompokan RAPD menggunakan metode Neighbour-Joining Tree yang membentuk tiga kelompok.
Jurnal Agroekoteknologi Universitas Sumatera Utara, Jun 8, 2016
The objective of this research was to study the efficacy of botanical pesticide Ocimum sanctum le... more The objective of this research was to study the efficacy of botanical pesticide Ocimum sanctum leaves on rice field rat mortality in laboratory. This research was conducted in Laboratory of Vertebrate Pest Control Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan (BP2TP), Medan (± 25 m asl) from December 2015 until February 2016.The method used Randomized Complete Design non Factorial with three replications. The results showed that O. sanctum leaves affected reduction in body weight of Rattus argentiventer. The highest bait consumption was showed on 60% O.sanctum in bait (19.33 g) and the lowest control (13.67 g) on first day after application. The highest 60% O. sanctum in bait mortality percentage (16.67%) and the lowest was showed on control (0.00%) on day eighteen after application. Behaviour of rats rest (46.64%), shiver (15.06%), along the floor (13.87%), bitting cage (8.39%), bitting food/water box (6.75%), repeat salto (5.79%), lack of appetite (1.86%), inactivity (0.98%), and bleeding on eye/nose (0.66%).
IOP Conference Series: Earth and Environmental Science, 2022
The coffee plant is a commodity that is widely grown in Toba Regency, Province of North Sumatera.... more The coffee plant is a commodity that is widely grown in Toba Regency, Province of North Sumatera. Coffee berry borer (CBB) Hypothenemus hampei (Coleoptera: Scolytidae) is an essential pest in coffee plants which can reduce the quantity and quality of coffee fruit up to 50%. This research was to study the use of attractants derived from extract coffee bean and outer skin (pericarp) at various height traps to control CBB in Parsaoran Sibisa Village, Lumban Julu District, Toba Regency. This research method uses a factorial randomized block design with two treatments and three replications. For the first factor is the type of attractants (aqua-des, coffee beans and pericarp). The second factor is the height of the trap (0.5; 1.0 and 1.5 m). The results showed that the interaction of attactant type and trap height had no significant effect, but the attractiveness of coffee beans significantly affected CBB population trapped. The attractant of coffee beans caught 16.40 adults, 6.57 adults...
Agroekoteknologi, Aug 27, 2013
PENGARUH PERANGKAP WARNA BERPEREKAT DAN AROMA REMPAH UNTUK MENGENDALIKAN HAMA GUDANG Lasioderma s... more PENGARUH PERANGKAP WARNA BERPEREKAT DAN AROMA REMPAH UNTUK MENGENDALIKAN HAMA GUDANG Lasioderma serricorne F. (Coleoptera: Anobiidae) DI GUDANG TEMBAKAU
Jurnal Online Agroekoteknologi, May 2, 2017
The objective of the research was to study the biology of insect pollinator Elaeidobius kamerunic... more The objective of the research was to study the biology of insect pollinator Elaeidobius kamerunicus after 33 years introduction in North Sumatera. The research was conducted at Indonesian Oil Palm Resesarch Institute (IOPRI), Marihat from March to May 2016. It was done by using descriptive method. The results showed that life cycle is 9-22 (+ 12.25) days; eggs 1-2 (+ 1.01) days, first instar larvae 1-2 (+ 1.05) days, second instar larvae 1-2 (+ 1.06) days, third instar larvae 2-11 (+ 4.75) days and pupae 2-3 (+ 2.35) days. Age of males 31-61 (+ 52.53) days longer than females 29-55 (+ 37.87) days.
Talenta Conference Series: Agricultural and Natural Resources (ANR), 2018
Penelitian untuk mengetahui toksisitas metabolit skunder Penicillium sp. pada berbagai media kult... more Penelitian untuk mengetahui toksisitas metabolit skunder Penicillium sp. pada berbagai media kultur yang akan digunakanuntuk mengendalikan Spodoptera sp. in vitro telah dilakukandi Laboratorium Penyakit Tumbuhan Fakultas Pertanian Universitas Sumatera Utara pada bulan Januari – Agustus 2016. Berbagai media biakan Penicillium sp. Yaitu Potato Dextrose Agar (Kontrol), Sabouraud Dextrose Agar, D0C2, Czapek Dox Agar, dan tepung yang dibuat dari tubuh Spodoptera sp. telah digunakan. Hasil penelitian menunjukkan media tepung yang dibuat dari tubuh Spodoptera sp. memiliki toksisitas tertinggi dengan persentase mortalitas 100% pada pengamatan jam ke enam. Lt50 didapat pada 3,545 jam.
Jurnal Agroekoteknologi Universitas Sumatera Utara, Jan 4, 2013
ABSTRACT Germination response of some rice (Oryza sativa L.) varieties to concentrations of NaCl.... more ABSTRACT Germination response of some rice (Oryza sativa L.) varieties to concentrations of NaCl. Land in Indonesia is very varied according to the character of the soil, such as acid soils, saline soil, andosol and others. Land use for rice planting resulted in the production of rice can not fulfill the population. For which, a study has been carried out at Seed Technology Laboratory, Faculty of Agriculture, University of Sumatera Utara, Medan with altitude ± 25 m asl, In May 2012 using a completely randomized factorial design 2 factors: varieties (Mekongga, Cigelis, Inpari 1, Cibogo and Ciherang) and NaCl concentrations (0, 1000, 2000, 3000 ppm). Peubah amatans measured were germination rate, germination percentage, sprouts percentage normal, radicle length, fresh weight sprouts. The results showed that the varieties did not significantly affect all peubah amatans were observed. NaCl concentrations significantly affect radicle length and fresh weight sprouts. Keywords: rice, varieties, NaCl concentration ABSTRAK Respons perkecambahan beberapa varietas padi ( Oryza sativa L.) terhadap berbagai konsentrasi garam NaCl. Tanah di wilayah Indonesia sangat beragam sesuai dengan karakter tanahnya masing-masing, misalnya tanah masam, tanah salin, tanah andosol dan lainnya. Ketersediaan lahan yang terbatas untuk pertanaman padi mengakibatkan produksi padi tidak dapat memenuhi jumlah penduduk. Untuk itu suatu penelitian telah dilakukan di Laboratorium Teknologi Benih, Fakultas Pertanian, Universitas Sumatera Utara, Medan dengan ketinggian tempat + 25 m dpl pada Mei 2012 menggunakan rancangan acak lengkap faktorial 2 faktor yaitu varietas (Mekongga, Cigelis, Inpari 1, Cibogo dan Ciherang) dan konsentrasi garam NaCl (0, 1.000, 2.000, 3.000 ppm). Peubah amatan yang diamati adalah laju perkecambahan, persentase perkecambahan, persentase kecambah normal, panjang radikula, berat basah kecambah. Hasil penelitian menunjukkan bahwa varietas tidak berpengaruh nyata terhadap semua peubah amatan yang diamati. Konsentrasi garam NaCl berpengaruh nyata terhadap panjang radikula dan bobot segar kecambah. Kata kunci : padi, varietas, konsentrasi garam NaCl
Jurnal Agroekoteknologi Universitas Sumatera Utara, Mar 2, 2013
The sugarcane stem borer Phragmatoecia castaneae (Lepidoptera : Cossidae) is a key pest on sugarc... more The sugarcane stem borer Phragmatoecia castaneae (Lepidoptera : Cossidae) is a key pest on sugarcane crop in North and West Sumatera. The objective of this research was to study the ability of parasitoid Tetrastichus sp. (Hymenoptera : Eulophidae) on pupa Ph. castaneae H. (Lepidoptera : Cossidae) in the laboratory. This research was carried out at Central Research and Development of Sugarcane Crop Sei Semayang PTPN II Medan from May to July 2012. The method of this research used Randomized Complete Design factorial with 2 factors, the first factor is number of parasitoid (0, 4, 5, 6, and 7 pairs) and the second factor is number of Ph. castaneae (1, 3, 5, 7, and 9 pupa) with three replications. The results showed that the highest percentage parasitisation (100%) on pupa Ph. castaneae was found in treatment 1 pupa with 5, 6, 7 pairs Tetrastichus sp. and the lowest percentage (18,50%) on 9 pupa with 4 pairs Tetrastichus sp. This parasitoid need 10-15 seconds into do copulation. Females of Tetrastichus sp. need 20-25 minutes to put egg into its host. The fastest symptoms in the treatment 1 pupa on 3,47 days and 7 pairs Tetrastichus sp. on 4,73 days, and the lowest in the treatment 9 pupa is 4,47 days. The sex ratio of male and female was 1:3,3. Key words : Phragmatoecia castaneae, Tetrastichus sp, ability of parasitoid.
IOP Conference Series: Earth and Environmental Science, 2022
Spodoptera litura F. is a new pest that potentially becomes a serious pest threatening palm oil i... more Spodoptera litura F. is a new pest that potentially becomes a serious pest threatening palm oil industry. The control can be carried out using biological control agents S.litura Nucleopolyhedrovirus (SlNPV). Molecular approach using polymerase chain reaction (PCR) has been used to characterize the SlNPV isolate. The aim of this study was to determine the molecular characteristics of SlNPV Labuhan Batu isolate based on DNA sequence analysis. The NPV isolate was collected from oil palm plantation Negeri Lama Estate Group, PT. Hari sawit Jaya, Asian Agri, Labuhan Batu. The SlNPV DNA extraction was conducted using the DNA gSYNC kit. The amplification of SlNPV DNA was done by specific primers with oligonucleotide sequences forward 5′-ACGTTACGATCTAGATCC-3′ and reverse 5′-AACGTTAGACAGACATAT-3′ with ±700bp of amplicon. Homology and phylogeny characteristics were used to determine the relationship of SlNPV isolate from Labuhan Batu to NPV from other countries were reported in the GeneBank of...
Uploads
Papers by maryani cyccu tobing