Papers by sushma pednekar
MedPulse International Journal of Microbiology, 2019
Journal of Evolution of Medical and Dental Sciences, 2016
Periodontitis is the most frequent oral health problem in the world. The infection is primarily c... more Periodontitis is the most frequent oral health problem in the world. The infection is primarily caused by anaerobic microorganisms. Metronidazole is the most commonly used drug to treat the infection but recently the anaerobes have shown the resistance to this drug. Therefore, the present study was undertaken to isolate and identify the anaerobes associated with periodontitis and study their susceptibility pattern to the Metronidazole. Total 90 samples were collected from chronic periodontitis cases. Anaerobes were isolated in 71% of periodontitis cases. Gram positive organisms were more predominantly isolated than Gram negative organisms. The Gram negative anaerobes were found to be 100% sensitive to Metronidazole while Gram positive anaerobes showed 8% resistance to Metronidazole.
Indian Journal of Medical Microbiology, 2017
Background: Diarrhoea remains the second most common cause of death among children below 5 years ... more Background: Diarrhoea remains the second most common cause of death among children below 5 years globally. Among various enteric pathogens, rotavirus appears to be the most important aetiological agent of acute gastroenteritis in infants and young children. Increased understanding of epidemiology of rotavirus infections is needed to improve the vaccine efficacy. Aim: This study aims to determine prevalence rotavirus infection and prevalent circulating strains of rotavirus in and around Pune. Setting and Design: Prospective hospital-based study. The study was approved by Institutional Ethical Committee. Materials and Methods: Stool samples (n = 100) were collected from children aged <5 years, hospitalised for acute diarrhoea in paediatric ward at a tertiary care hospital. Samples were subjected for rotavirus antigen capture ELISA. The viral RNA was subjected to multiplex reverse transcription polymerase chain reaction to amplify VP7 genotypes G1–G4, G8–G10 and G12 and VP4 genotypes P[4], P[6], P[8], P[9], P[10] and P[11]. Nontypable rotavirus strains were sequenced. Results: About 35% stool samples were positive for rotavirus antigen by ELISA. G9P[4] (28.6%) was found to be the most prevalent rotavirus strain. The detection of emerging strain G12P[6] (14.3%) and rare reassortant strain G9P[4] was the significant finding. Conclusion: Genotypes found in circulation are not present in the currently used vaccine. Thus, an emergence of newer genotypes over a period calls for the continued surveillance and genomic characterisation of rotaviruses to improve the vaccine efficacy.
Introduction: Poorly controlled diabetes and associated peripheral neuropathy often results in fo... more Introduction: Poorly controlled diabetes and associated peripheral neuropathy often results in foot ulcers which get infected frequently resulting in gas gangrene and sometimes require amputation. Polymicrobial nature of infection is usually considered to start the empirical treatment. The aerobic culture and sensitivity is commonly practiced to guide the treatment but no efforts are taken to look for the anaerobic infection. The upcoming resistance of anaerobes to the commonly used antibiotics is under diagnosed leading to treatment failure in diabetic foot ulcers. The present study was undertaken to investigate the role of anaerobic infections in 50 clinically diagnosed diabetic foot ulcer patients admitted in a tertiary care hospital. Aims & Objectives: To isolate & identify the aerobic & anaerobic bacteria from diabetic foot ulcers as per the standard microbiological technique. To study the antimicrobial susceptibility pattern of both aerobic & anaerobic bacteria isolated from t...
INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH, 2021
Fonsecaea pedrosoi (F. pedrosoi) is dematiaceous fungus and is the most common cause for chromobl... more Fonsecaea pedrosoi (F. pedrosoi) is dematiaceous fungus and is the most common cause for chromoblastomycosis. It affects the exposed skin, mostly of the lower extremities. Arare case of mycotic keratitis was diagnosed in our hospital caused by F. pedrosoi. Corneal sample received in the laboratory was processed by standard mycological methods, F. pedrosoi was isolated, patient was started on antifungals his condition improved and there was no relapse. This case report shows that F. pedrosoi can infect cornea. Further, a prompt diagnosis and vigorous treatment improves patient's clinical condition.
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Papers by sushma pednekar