Papers by sorayya ghasemi
EXCLI Journal, 2022
Polycystic ovary syndrome (PCOS) is the most common cause of women's infertility. Some inflam... more Polycystic ovary syndrome (PCOS) is the most common cause of women's infertility. Some inflammatory pathways play a pivotal role in the pathogenesis of PCOS. This study aimed to investigate the possible beneficial effects of minocycline on chemokine-like receptor 1 (CMKLR1) and Insulin Receptor (INSR) in a PCOS model. A molecular docking study was implemented using Molecular Operating Environment (MOE) software. The PCOS was induced in NMRI mice (mean body weight 14.47±0.23) by 28 days estradiol valerate injection (2 mg/kg/day). The mice were then divided into six groups (n=8 per group, mean body weight 17.77± 0.26): control (received normal saline), PCOS model, control for minocycline, minocycline treated PCOS (50 mg/kg), letrozole treated PCOS (0.5 mg/kg), and metformin-treated PCOS (300 mg/kg). Serum FSH, LH, estradiol (E2), and testosterone were detected by ELISA. The ovarian tissues were stained by hematoxylin and eosin. The CMKLR1 and INSR expression levels were determined...
Current Drug Targets
Background: A large number of studies have been conducted on the treatment of glio-blastoma multi... more Background: A large number of studies have been conducted on the treatment of glio-blastoma multiforme (GBM). Chemotherapeutic drugs cannot penetrate deeply into the brain paren-chyma due to the presence of the blood-brain barrier (BBB). Hence, crossing BBB is a significant obstacle in developing new therapeutic methods for GBM. Objective: Cell-penetrating peptides (CPPs) have emerged as new tools that can efficiently deliver various substances across BBB. CPPs beneficial properties, such as BBB penetration capacity, low toxicity, and the ability to achieve active targeting and controllable drug release, have made them worthy candidates for GBM treatment. However, their application is limited by several drawbacks, including lack of selectivity, insufficient transport efficacy, and low stability. In order to overcome the selectivity issue, tumor targeting peptides and sequences that can be activated at the target site have been embedded into the structure of CPPs. To overcome their i...
BACKGROUND AND OBJECTIVE: Tricine, as a flavonoid compound in many food sources, has anti-inflamm... more BACKGROUND AND OBJECTIVE: Tricine, as a flavonoid compound in many food sources, has anti-inflammatory and anti-proliferative effects in some cancer cell lines. Considering the importance of using natural anti-cancer drugs in therapy-resistant cancers such as prostate cancer, the aim of this study is to investigate the effect of tricine on cell growth and proliferation and induction of apoptosis in PC3 human prostate cancer cell line. METHODS: In this experimental study, the PC3 cell line was prepared and cultured from the Pasteur Institute of Iran. Extraction and purification of tricine were done by column chromatography and recrystallization of Allium atroviolaceum extract. The apoptotic effect of tricine at concentrations of 60, 80, 100, 120 and 140 μM was evaluated by MTT method. The apoptotic effect was evaluated in the cell group treated with IC50 concentration of tricine and untreated cells (control group) using Annexin-V kit and flow cytometry. FINDINGS: The viability of cel...
Nanomaterials
Resistance to temozolomide (TMZ) is the main cause of death in glioblastoma multiforme (GBM). The... more Resistance to temozolomide (TMZ) is the main cause of death in glioblastoma multiforme (GBM). The use of nanocarriers for drug delivery applications is one of the known approaches to overcome drug resistance. This study aimed to investigate the possible effect of selenium–chitosan nanoparticles loaded with TMZ on the efficacy of TMZ on the expression of MGMT, E2F6, and RELA genes and the rate of apoptosis in the C6 cell line. Selenium nanoparticles (SNPs) were loaded with TMZ and then they were coated by Eudragit® RS100 (Eud) and chitosan (CS) to prepare Se@TMZ/Eud-Cs. Physicochemical properties were determined by scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDAX), thermal gravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) methods. Se@TMZ/Eud-Cs was evaluated for loading and release of TMZ by spectrophotometric method. Subsequently, SNPs loaded with curcumin (as a fluorophore) were analyzed for ...
Journal of Shahrekord University of Medical Sciences, 2014
Background and aims: P19 cells are a line of pluripotent embryonal carcinoma stem cells that are ... more Background and aims: P19 cells are a line of pluripotent embryonal carcinoma stem cells that are able to grow continuously in serum-supplemented media and can be induced to differentiate into all three lineages, mesoderm, endoderm and ectoderm. The sequence of foreign DNA can be introduced into these cells using of standard methods of transfection, through examineing the cell function and differentiation. This study aimed to induce neuronal phenotype in P19 cells transfected with green fluorescent protein (GFP) gene, using an antiparkinson drug, deprenyl. Methods: In this laboratory descriptive study, calcium phosphate precipitation method was used to introduce pML8 plasmid, encoding GFP and puromycin resistance gene, into these cells. The cells were cultured using MEM-A medium, supplemented with 15% fetal bovine serum. Here 10 8 M concentrations of deprenyl were used to induce embryoid body differentiation to neuronal lineage. Cresyl violet staining was used to evaluate the morphology of differentiated P19 cells. In addition, immunofluorescence techniques was used to evaluate neuron specific proteins, synaptophysin and β-III tubulin. Results: In addition to production of stable transfected P19 cells, neuronal differentiation of these cells was carried out by the effect of deprenyl induction. GFP-positive neurons derived from embryonic carcinoma cells expressed neuron specific markers. Conclusions: GFP-positive differentiated cells can be used in transplantation studies and basic researches in association with cell therapy and neurodegenerative diseases.
Journal of reproductive immunology, 2021
Polycystic ovary syndrome (PCOS) is the most common cause of ovulatory infertility. Inflammation ... more Polycystic ovary syndrome (PCOS) is the most common cause of ovulatory infertility. Inflammation may be involved in the pathogenesis and development of PCOS. We investigated the anti-inflammatory effect of minocycline on TNF-α, TNFR2, and TLR4 expression levels and the key features of PCOS in a mouse model. Molecular docking was performed by Molecular Operating Environment software. PCOS was induced by estradiol valerate injection (EV) (2 mg/kg/day) in 40 mice. After 28 days, the mice were divided into five groups, including control, PCOS, minocycline control, minocycline PCOS model (50 mg/kg), and letrozole PCOS (0.5 mg/kg). The Levels of FSH, LH, E2, and testosterone were determined by ELISA. H&E staining was used for histological analysis in the ovarian tissues. Docking scores were -10.35, -10.57, and -12.45 kcal/mol for TNFα, TLR-4, and TNFR2, respectively. The expression levels of TNF-α, TNFR2, and TLR4 were detected by Real-Time PCR. PCOS models exhibited acyclicity, a signifi...
Genetics in the Third Millennium, 2007
Retinoic acid (RA) is a vitamin A derivative that exerts profound influences in vertebrate develo... more Retinoic acid (RA) is a vitamin A derivative that exerts profound influences in vertebrate development and physiology. All-trans RA and 9-cis RA, are important regulators of embryonic development, cellular activity and tissue homeostasis. Their biological effects are mediated by two families of nuclear hormone receptors, retinoic acid (RAR) and retinoid X (RXR) receptors, which are ligand-dependent transcription factor. In the form of RAR-RXR or RXR-RXR dimers, the receptors act through binding to retinoic acid response elements (RAREs) present in the transcription regulatory region (promoter) of target genes. The identification of the pattern of expression of each isoform is crucial to our complete understanding of retinoid physiology. Compound null mutations of retinoic acid receptor (RAR) genes lead to lethality in uterus or shortly after birth and to numerous developmental abnormalities. The role of RXRs in the mediation of the developmental retinoid signal is less clear, becaus...
Iranian Journal of Medical Education, 2017
دستیابی به تکنولوژیهای جدید در علوم زیستی و پزشکی، همچون توالییابی نسل بعد، زمینه پیشرفت سریع... more دستیابی به تکنولوژیهای جدید در علوم زیستی و پزشکی، همچون توالییابی نسل بعد، زمینه پیشرفت سریعتر از پیش مطالعات را فراهم نموده است. از این رو، حجم بسیار عظیمی از اطلاعات اولیه فراهم میگردد و همچنان نیز رو به افزایش است. طراحی سیستمهای پیشرفتهای که تلفیقی از محاسبات کامپیوتری، علم آمار، تکنولوژی اطلاعات وهوش مصنوعی باشند، جهت ذخیره، پردازش و تفسیر این اطلاعات روبه افزایش، الزامی است(1). علوم ذکرشده با عنوان ابزارهای بیوانفورماتیکی جهت حل مسائل پیچیده زیستی و نتیجهگیری منطقی به خدمت گرفته میشوند. ژنومیک، ترانس کریپتومیک، پروتئومیک و متابولومیک، زمینههای اصلی مطالعات بیوانفورماتیک تلقی میگردند(2). امروزه، ابزارهای بیوانفورماتیکی متعددی به صورت دسترسی آزاد وonline توسط پایگاه دادهها و نرمافزارهای تحت وب، یا به صورت قابل ذخیره و اجرا در ویندوز با عنوان نرمافزار دسک تاپ و یا نرمافزارهایی قابل خریداری در اختیار محققان قرارگرفتهاند(3). ظهور ابزارهای بیوانفورماتیکی و محاسبات کامپیوتری انقلابی در مطالعات زیستی و پزشکی ایجادکرده است. این ابزارها برای ذخیره، مدیریت، پردازش و تجزی...
The clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 (CRISPR... more The clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 (CRISPR-Cas9) used for genome editing. The usage of CRISPR-Cas9 in gene editing is faced with certain limitations including off-target mutation, decreased homologous recombination (HR) repair, and immune system responses. It seems that if Cas9 expressed in an inducible manner, off-target mutations may decrease. P53 decreases the activity of the HR pathway in the cell cycle, so, the decrease in P53 level may increase the activity of this pathway. Based on this topic, for the first time, we designed ''px601-Turbo GFP-TRE-shRNA P53'' as a CRISPR-based vector. The use of this vector can simultaneously induce expression of Cas9 and shutdown transiently P53 under an inducible promoter and an inducing agent. Therefore, shutdown transiently P53 may be leading to reduced off-targets and increased accuracy of genome editing. In the human gastric cancer MKN45 cell line, the P53 gene expres...
Prostate cancer is the third most common cancer among men. Risk factors for prostate cancer conta... more Prostate cancer is the third most common cancer among men. Risk factors for prostate cancer contain environmental factors such as diet rich of reactive oxygen species. Epidemiological studies recommend dietary consumption of plant-derived foods may decrease the risk of prostate, colon, pancreas, breast and lung cancers. However, it has been difficult to identify specific components responsible for this effect. Many phytochemicals have been shown to be biologically active compounds and they may act as a protective factor against cancer. Recent researches have revealed that diets based on flavonoids have the potential benefits for cancer prevention. Flavonoids are part of a large class of polyphenols that are found widely in diet. Nowadays, experimental researches have suggested developing proof for the valuable action of flavonoids on multiple cancer-related biological pathways. Although the outcomes of studies about flavonoids and prostate cancer are still limited and conflicting, s...
Background: Stress gradually suppresses the immune system making the body susceptible to various ... more Background: Stress gradually suppresses the immune system making the body susceptible to various diseases. The aim of this study was to show the effects of cold and immobilization stress on growth of malignant cells in Balb/c mice. Methods: In an experimental study which was carried out in the Molecular & Cellular Research Center of Shahrekord University of Medical Sciences in 2008, 3 groups of 10 Balb/c mice were selected to investigate the effects of cold stress and immobilization stress on growth of WEHI – 164 fibrosarcoma cells in groups of 10, Balb/c mice. Each mouse was injected with tumor cells subcutaneously in the chest for 2 hours/day mice in group 1were subjected to cold stress and mice in group 2 were immobilized the control group received tumor cells with no stresses. Tumor sizes were measured every two days and case groups were compared with the control group. Results: Results showed that the tumor sizes in stressed mice were significantly different from controls. Afte...
BACKGROUND AND OBJECTIVE: The expression of some circulating microRNAs (miRNAs) in biological flu... more BACKGROUND AND OBJECTIVE: The expression of some circulating microRNAs (miRNAs) in biological fluids of healthy individuals is different from cancerous patients. circulating miRNAs are a new class of cancer biomarkers because of their high stability and sensitivity, ease of measurement and specificity due to their correlation with various cancer states. According to the miR-451 and miR-21 functions in the metastasis of some cancers, the aim of this study was to investigate the differences of expression levels of miR-451 and miR-21 in the plasma of breast cancer (BC) patients with and without lymph nodes metastasis (LNM). METHODS: In this descriptive-analytical study, blood samples were collected from 47 women with BC and 24 healthy women with mammography confirmation. The presence/or absence of LNM was recognized from patients' medical records. The expression levels of miR-451 and miR-21 in the plasma, were investigated using Real-Time PCR. FINDINGS: The median of expression of ...
Cancer is caused by aberrant genetic and epigenetic changes in genes expression. DNA methylation,... more Cancer is caused by aberrant genetic and epigenetic changes in genes expression. DNA methylation, histone modification, and microRNAs gene deregulation are the most known epigenetic changes in different stages of cancer. Since every tumor has its own specific epigenome, any abnormal pattern is a potential biomarker for classification of different types of tumors. Despite, tumorigenesis, abnormal epigenetic changes are highly correlated with drug resistance in various stages of cancer. But, reversible nature of these abnormalities is the basis of epigenetic cancer treatment. Drugs affecting the epigenome are the new hopes in cancer treatment. The aim of this study was to investigate the role of epigenetics in tumorigenesis and also drug resistance in cancers.
International Journal of Molecular and Cellular Medicine (IJMCM), 2020
Polycystic ovary syndrome (PCOS) is a gynecological endocrine disorder in women of reproductive a... more Polycystic ovary syndrome (PCOS) is a gynecological endocrine disorder in women of reproductive age. There is adequate evidence that suggests several microRNAs (miRNAs) are of great importance for PCOS. It seems that dysregulated expression of miR-27a, miR-130b, and miR-301a are associated with PCOS. The aim of this study was to investigate whether plasma levels of these miRNAs are different between patients with PCOS and healthy controls. 53 women with a definite diagnosis of PCOS, and 53 healthy controls were enrolled. MiRNAs expression levels in plasma were evaluated by real-time PCR. The diagnostic values of each miRNA were calculated by the receiver operating characteristic (ROC) curve and areas under the curves (AUC). The main clinical characteristics were not significantly different between the two groups. The circulating plasma expression levels of miR-27a and miR-301a had a significant increase (P = 0.0008 and P < 0.0001, respectively) but miR-130b expression level decre...
Peroxisomes are organelles present in all eukaryotic cells from yeast to human cells. It is now w... more Peroxisomes are organelles present in all eukaryotic cells from yeast to human cells. It is now well known that approximately fifty different biochemical reactions occur within the peroxisome, including synthesis of bile acids, cholesterol, ether-phospholipids (plasmalogens), docosahexaenoic acid and catabolism of certain fatty acids, particularly very long chain fatty acids (VLCFAs). Proteins involved in peroxisomal function are known as peroxins. At least 29 peroxins are required for peroxisome membrane biogenesis, fission, and protein import. So far, mutations in 13 genes that encode peroxins are associated with human disease. Peroxisomal disorders currently falling into one of three groups peroxisome biogenesis disorders (PBDs), peroxisomal multi-enzyme disorders, and peroxisomal single-enzyme disorders. Infantile Refsum’s disease (IRD), neonatal adrenoleukodystrophy (NALD) and Zellweger’s syndrome (ZS) are different variants of a group of congenital diseases known as peroxisome...
Glioblastoma multiforme (GBM) resistance to anti-angiogenesis drugs results in recurrence of the ... more Glioblastoma multiforme (GBM) resistance to anti-angiogenesis drugs results in recurrence of the disease which leads to death. The resistance to anti-angiogenesis drugs that target the VEGF pathway is due to the influence of other pathways. This study aimed to identify and classify the pathways that are related to the VEGF pathway in GBM recurrent. The identification of differentially expressed miRNAs (DEmiRNAs) based on GBM GSE profiles (GSE32466) were carried out using a LIMMA R package and VEGF pathway genes in the KEGG database. Pathways related to DEmiRNAs and VEGF pathway genes were discovered by DIANA-miRPath v3.0, NetworkAnalyst and ToppGene databases, respectively. Inhibitory or activity affecting pathways relating to VEGF pathway were obtained based on XTalkDB database. The classification was determined by the KEGG database. There were 1014 genes that were found to have interaction with VEGF signaling pathway genes. One hundred ninteen pathways were achieved which have ove...
Current Molecular Medicine
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) has high mortality due to... more Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) has high mortality due to the widespread infection and the strong immune system reaction. Interleukins (ILs) are among the main immune factors contributing to the deterioration of the immune response and the formation of cytokine storms in coronavirus disease 2019 (COVID-19) infections. Introduction: This review article investigated the relationship between virus structure, risk factors, and patient plasma interleukin levels in infections caused by the coronavirus family. Method: The keywords "interleukin," "coronavirus structure," "plasma," and "risk factors" were the main words searched to find a relationship among different interleukins, coronavirus structures, and risk factors in ISI, PUBMED, SCOPUS, and Google Scholar databases. Results: Patients with high-risk conditions with independent panels of immune system markers are more susceptible to death caused by SARS-CoV...
Evidence-Based Complementary and Alternative Medicine
Rutin is a unique antioxidant flavonoid that is mainly found in fruit, vegetables, cereals, and m... more Rutin is a unique antioxidant flavonoid that is mainly found in fruit, vegetables, cereals, and many other plant-based human diets. This review aims to highlight the in vitro anticancer properties of rutin including combination therapeutic strategies. Literature resources were gathered through PubMed, Scopus, Web of Science, and Google Scholar databases that cover the period of 1995–2021. Rutin is demonstrated to inhibit the proliferation of breast, colon, lung, and prostate cancers and other tumors. Furthermore, rutin alone or in combination with other therapeutic agents has been shown to regulate several signalling pathways involving the Ras/Raf and PI3K/Akt, MAPK, and TGF-β2/Smad2/3Akt/PTEN, etc., which are related to the processes of carcinogenesis and induction of apoptosis. The combination of rutin with other chemotherapy drugs may benefit on prevention of tumor cells by decreasing drug resistance and chemotherapy side effects. Moreover, rutin induces apoptosis synergistically...
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Papers by sorayya ghasemi