Papers by salman ahmady asbchin
Probiotics and Antimicrobial Proteins, 2016
The main drawback of current antibiotic therapies is the emergence and rapid increase in antibiot... more The main drawback of current antibiotic therapies is the emergence and rapid increase in antibiotic resistance. Nocardiae are aerobic, Gram-positive, catalase-positive, non-motile actinomycetes. Nocardia brasiliensis was reported as antibiotic producer. The purpose of the study was to determine antibacterial activity of N. brasiliensis PTCC 1422 against isolated Enterobacteriaceae from urinary tract infections (UTIs). The common bacteria from UTIs were isolated from hospital samples. Antimicrobial susceptibility test was performed for the isolated pathogens using Kirby–Bauer disk diffusion method according to clinical and Laboratory Standards Institute guideline. Antagonistic activity of N. brasiliensis PTCC 1422 was examined with well diffusion methods. Supernatant of N. brasiliensis PTCC 1422 by submerged culture was analyzed with gas chromatography–mass spectrometry. Isolated strains included Escherichia coli, Klebsiella pneumoniae, Serratia marcescens and Proteus mirabilis. The most common pathogen isolated was E. coli (72.5 %). Bacterial isolates revealed the presence of high levels of antimicrobial resistances to ceftriaxone and low levels of resistance to cephalexin. Supernatant of N. brasiliensis PTCC 1422 showed antibacterial activity against all of the isolated microorganisms in well diffusion method. The antibiotic resistance among the uropathogens is an evolving process, so a routine surveillance to monitor the etiologic agents of UTI and the resistance pattern should be carried out timely to choose the most effective empirical treatment by the physicians. Our present investigation indicates that the substances present in the N. brasiliensis PTCC 1422 could be used to inhibit the growth of human pathogen. Antibacterial resistance among bacterial uropathogen is an evolving process. Therefore, in the field on the need of re-evaluation of empirical treatment of UTIs, our present. The study has demonstrated that N. brasiliensis PTCC 1422 has a high potential for the treatment of UTIs.
Helminthes are important cause of reducing the body weight and producing the disease, anemia in y... more Helminthes are important cause of reducing the body weight and producing the disease, anemia in young or debilitated animals. The aim of this study is to perform experimental research to obtain the effects of plant extraction of Fumatiaceae on control of Trichostrongylus axei in infected goats and its effects on weight gain and hematological parameters changes due to this parasitic infection.In Ilam province Iran were this experiment had been conducted the farmers traditionally climes that this plant could be use as a anthelmintic medicine. Twenty four draft goats, 10-12 months of age and with the average weight of 15.550 kg divided in two groups (group 1 control and the group 2 as a experimental animal infected with T. axei (5000L3) orally and after 10 weeks of parasitic infection the experimental group of animals were de wormed with 6ml/kgG body weight 1 Fumatiaceae plant extraction. Plasma of blood sample was separated for determination of total protein, plasma total free amino a...
World Journal of Microbiology and Biotechnology
The environmental contamination of heavy metals has grown over the last several decades along wit... more The environmental contamination of heavy metals has grown over the last several decades along with global industrializa- tion and now constitutes a serious threat to human health. In this research, high cadmium (Cd) and nickel (Ni) resistant bacteria that were chosen for heavy metal biosorption were isolated from the Industries Factory in Sari, Mazandaran, Iran. Pseudomonas aeruginosa was recognized as the isolated bacterium based on its morphological, physiological, biochemical, antibiotic resistance testing, and 16S rRNA sequences. The bacteria had the highest concentrations of resistance to Cd (up to 1600 ppm) and Ni, according to the Minimum Inhibition Concentration (MIC) test (up to 2000 ppm). Single-factor studies in single and binary systems were used to examine the effects of temperature, contact duration, pH value, starting Cd and Ni concentration, and biomass dose on the Cd and Ni adsorption by P. aeruginosa. The Cd and Ni biosorption in binary solutions was optimized using the response surface methodology (RSM) based on Central Composite Design (CCD). The investigation revealed that at pH 7.0, 45 °C, and 1.5 gL−1 biomass dose, the greatest biosorption efficiency for Cd and Ni was 92.43 percent and 88.45 percent, respectively. According to the adsorption of Cd and Ni in urban water, under these similarly extreme conditions, Cd adsorption drops to 54% and Ni to 60%. Analysis Potential functional groups involving interactions between cells and metal ions were identified using Fourier transform infrared spectroscopy (FTIR). Different compounds and heavy metal ions were found to have been adsorbed to the surface of the biosorbent by scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDS). It was determined that P. aeruginosa had a high rate of Cd and Ni adsorption and that variations in pH level had a greater impact than other parameters. The findings imply that P. aeruginosa biomass may be an effective, economical, and environmentally friendly method for removing Cd and Ni from contaminated settings. It also has a reasonable capacity for biosorption in both a natural environment and a laboratory environment.
Bioremediation Journal, Nov 1, 2022
DESALINATION AND WATER TREATMENT
Assisted phytoremediation technique is greatly progressed, but is still in development and needs ... more Assisted phytoremediation technique is greatly progressed, but is still in development and needs further study. Thereby, in this study, Ligustrum texanum (Wax-leaf Privet) was exposed to soil treated with the various concentrations of lead nitrate (Pb(NO3) 2) (0, 100, 300, 500, and 700 mg L-1) and humic acid (HA) (0 and 300 mg L-1) irrigation solution for the three-month period, in order to evaluate the species' ability to phytoremediation of soil Pb 2+ ; and the effect of HA on this process. Un-planted pots were also used and treated with Pb in order to investigate the direct role of plants in soil remediation. The effect of Pb and HA on various plant morphological traits (plant tissue's fresh and dry weight, plant and root length, number of leaves, and collar diameter) were also detected. Up to 200 µg g-1 Pb in soil (as a result of 500 mg L-1 Pb irrigation solution), Pb led to a positive (stimulative) effect on various plant morphological traits, in contrast, Pb in higher concentrations had a negative (repressive) effect on plant growth. Humic acid had increased plant morphological traits whilst is not promising for contaminated soil phytoremediation process due to reducing uptake and accumulation of Pb through plant roots. According to the results, this evergreen species is prominent for Pb phytoremediation in contaminated soils due to its high tolerance to Pb contamination, high Pb uptake, and plant affordability.
A We have presented a comparative study of silver nanoparticles (NPs) and corona discharge for de... more A We have presented a comparative study of silver nanoparticles (NPs) and corona discharge for decomposition of Eosin Y (EY) as a model dye and bactericidal activity of a series of gram-positive and gram-negative bacteria as representative microorganisms. Despite the fact that silver NPs demonstrate a great antibacterial activity, corona discharge process showed a significant decrease in survived bacteria in a few minutes. Furthermore, the results revealed that corona discharge at ambient air significantly enhanced degradation rate of model dye compared to silver NPs. Dye removal was about 88% after 10 min in corona discharge process compared to 36% dye removal after 2 h in the presence of silver NPs. It was suggested that corona discharge treatment, which is based on the most efficient energetic species, was more powerful than silver NPs in environmental and antibacterial applications which shown potential to kill different bacteria and degrade EY dye in less than 10 min.
journal of ilam university of medical sciences, 2020
The present study aimed to investigate the association of oral administration of cereal grain and... more The present study aimed to investigate the association of oral administration of cereal grain and seeds including wheat, alfalfa, barley, oat, red bean, white bean, clover, corn, pea, lentil, chickling, vetch and the function of avian spermatozoa morphology and sperm motility, sperm ejaculate, phospholipids content and thiobarbituric acid substances. During a period of 50 weeks time, 100 Kurdish cocks were equally divided in 5 dietary groups. The first group (control) fed routine diet. Enrichment was made on the diet of the 2 nd , 3 rd , 4 th and 5 th groups by 100g, 200g, 300g and 400g, germ of cereal grains and seeds respectively. The outcomes of conduction tests were pooled semen and testes’ weight. Weight of testes decreased by increasing the amount of given germ of cereal grains and seeds (p<0.05). The total amount of spermatozoa and morphology of sperm were decreased by adding more amount of germ of cereal grain and seeds (p<0.01). Increased germ of cereal grain and seed...
Methods of physical and chemical adsorption of heavy metals have disadvantages in some ways- such... more Methods of physical and chemical adsorption of heavy metals have disadvantages in some ways- such as high cost and ineffectiveness at low concentrations. In recent decades methods of biological uptake of heavy metals have been investigated. The biological adsorbents include bacteria, fungi and alga, among which algae have the highest efficiency of metal uptake. This study indicated that Alginic acid is most responsible for the uptake of metals, such as nickel (II) and cadmium (II). Fucus serratus, brown alga, used as a cost-effective adsorbent for the biological uptake of cadmium and nickel ions simultaneously in a batch reactor in this study. Surface structure of algae has also been investigated. Adsorption kinetics have been measured and the results have indicated that the equilibrium time is about 300 minutes. The adsorption isotherm was interpreted by means of the Langmuir equation. The maximum adsorption rate for cadmium (II) and nickel (II) turned out to be about 0.85 and 0.95...
European Journal of Biological Research, 2020
Among biodegradable plastics polyhydroxy alkanate and its polymers have received more attention t... more Among biodegradable plastics polyhydroxy alkanate and its polymers have received more attention than other biodegradable polymers because of their complete degradability, flexibility, water resistance and also the ease of production process. Polyhydroxybutyrate is one of the types of polyhydroxy alkanates that is seen as a storage granule in many microorganisms. In this study, Bacillus megaterium was prepared from Iranian microbial collection. Glucose and yeast extract were used as the main components of the medium in seed media 9 and 2.5 g/l and in fermentation medium 30 and 7.5 g/l respectively. GC-MASS and FTIR were used to identify the PHB produced. The results showed that the highest amount of biomass (0.221 g/l) and PHB (0.080 g/l) were obtained with glucose at 37°C and shaker speed of 150 rpm for 72 h incubation. The results of GC MASS and FTIR showed the production of PHB by Bacillus under investigation. Based on the mean of data on total cell growth conditions, the rate of ...
International Journal of Molecular and Clinical Microbiology, 2011
Article history: Received 26 November 2011 Accepted 5 December 2011 Available online 28 Dec 2011 ... more Article history: Received 26 November 2011 Accepted 5 December 2011 Available online 28 Dec 2011 Clinical signs, para-clinical tests and gross lesion can be used for identification of parasite contamination. In most parasite contamination the clinical signs are not obvious. Thus, para-clinical tests and gross lesion play important roles in identifying parasite disease as all organs of animals such as skin, eyes, kidneys, liver and digestive tube can be affected by the parasites. Consequently, there is a possibility to use the feces of animals in order to observe parasite ovum and larva to identify the disease. This research was conducted from May to September 2011, and the number of 250 faecal samples of pigeon from mixed companion birds keeping in the cages. Faecal samples were examined by direct smear method whereas egg per gram (EPG) was counted by modified McMaster technique and centrifugal flotation method using Sheather's saturated sugar solution. In this study, Raillietin...
aim of this work was to investigate Bacillus sp. strain MGL-75 as biosorbent, for the fixation of... more aim of this work was to investigate Bacillus sp. strain MGL-75 as biosorbent, for the fixation of Ni ion in batch reactor. Pollution of the environment by toxic metals is a major environmental concern. In a first step, biosorption kinetics and isotherms have been performed at pH 7. The equilibrium time was about 5 min and the adsorption equilibrium data were well described by the Langmuirs equation. The point of zero net proton charge (PZNPC) was found close to pH 5.7. Using the single extrapolation method, three kinds of acidic functional groups with three intrinsic pka were determined at 4.4, 6.9 and 11.2. The maximum capacity has been extrapolated to 0/52 mmol/g. Finally the effect of autoclave, 2, 4 Dinitrophenol (DNF) and Na-Azid (NaN3), and the effect of pH values, were studied. These results indicated that the Bacillus sp. strain MGL-75 is an excellent candidate for use in reactor to remove Nickel ions from polluted aqueous effluents.
scientific journal of ilam university of medical sciences, 2014
Introduction: Heavy metals are the most important bioenvironmental pollutants that are a serious ... more Introduction: Heavy metals are the most important bioenvironmental pollutants that are a serious threat for human health and aquatic systems. In the present research, the biosorption of heavy metals by Saccharomyces carlsbergensiswas (S. carlsbergensis) evaluated. Materials & Methods: The yeast, S. carlsbergensis PTCC 5051 was prepared as lyophilized powder and was cultured in the YEDPA medium. Then, for the proliferation of the yeast malt extract broth was used. The effect of pH, temperature, kinetics and isotherm parameters on the absorption of copper by S. carlsbergensis was evaluated. Findings: Maximum absorption was observed at pH 5 and temperature 25°C. Kinetic study showed that biosorption of copper was occurred by the biomass of S. carlsbergensis and the absorption was done in the first 30 minutes of the experiment. Surface functional groups of the fungus were identified by using FT-IR method. By comparing the absorption of copper by active and passive S. carlsbergensis, it ...
Biosorption of Nickel was studied by using free with variation in the parameters of pH, initial m... more Biosorption of Nickel was studied by using free with variation in the parameters of pH, initial metal ion concentration and agitation speed, and immobilized biomass of Bacillus sp. strain MGL-75 at pH 7. The aim of this work was to find the biosorption characteristics of selected a biomaterial against to heavy metals for the removal of nickel ions. The obtained results showed that, the alginate calcium, entrapped microorganism (Bacillus sp.) is found as a good adsorbing medium for these metal ions and has high adsorption yields for the treatment of wastewater containing nickel ions, as compared to beads without biomass and cell free. Biosorption of metal ions on alginate beads was investigated by using a batch stirred system at pH 6.0, 25°C, in initial metal concentration of 0.4 mmol/l of nickel ions. The equilibrium biosorption level was determined as a function of contact time at several initial metal ion concentrations. All these observations indicate that the nickel biosorption ...
Arak Medical University Journal, 2014
Background: Biosurfactants are amphiphilic biological compounds produced extracellularly by a var... more Background: Biosurfactants are amphiphilic biological compounds produced extracellularly by a variety of microorganisms. Because their use in various industries is of a particular importance, the aim of this study was to identify a strain of bacteria of the genus Bacillus Cereus biosurfactant producers and investigate the antibacterial effects of their biosurfactants. Material & Methods: To do the study, different samples of oil, water, and soil (contaminated with oil) were obtained. Measures of hemolytic activities, emulsification and surface tension were used and selected strains were identified by biochemical tests. The nature and antibacterial effect of biosurfactants produced by selected strains were assessed. Results: In this study, 88 bacterial strains were isolated of which 24 strains had hemolytic activities, 14 strains had above 70% emulsification activities, and four strains were able to change the surface tension to less than 40 mN/m. According to biochemical tests, a st...
Biomedical Research and Clinical Practice, 2020
In this present study the effects of pH, temperature, single and dual metal ion concentrations, d... more In this present study the effects of pH, temperature, single and dual metal ion concentrations, dose of Planococcus biomass and desorption agents on the uptake of Cd(II) and Ni(II) was measured using atomic absorption spectrophotometry. To show surface adsorption of bacteria, SEM-EDX and FTIR analysis were done before and after metals adsorption. The maximum biosorption capacity of Planococcus sp determined as 0.67, 0.48 mmol g −1 for cadmium(II) and as 0.58, 0.47 mmol g −1 for nickel(II) for single and binary ion situation. The correlation coefficient for the second-order kinetic model was 0.993. The Langmuir and Freundlich isotherm models were also applied to the equilibrium of system and data were better fitted with the Langmuir isotherm.
Microbiology Research, 2017
High occurrence of antibiotic resistance in pathogenic bacteria is caused by the use of natural m... more High occurrence of antibiotic resistance in pathogenic bacteria is caused by the use of natural medicinal plants to eliminate risk of infectious diseases. Glycine max is on the most popular and nutritious foods with high antimicrobial effects. The present research was done to study the antibacterial effects of the methanolic extract of G. max against Gram-negative and positive bacteria. G. max seeds of M7 and M9 varieties were purchased and their methanolic extracts were collected. Diameter of the zone of growth inhibition of extracts were measured for pathogenic bacteria using the CLSI guidelines. Antimicrobial effects of G. max extracts were also compared with several antibiotics. Diameter of the inhibition zone of tested bacteria against different treatments of G. max had a ranges of 5.93±0.18 to 22.61±1.80 mm. M7 variety had a higher antimicrobial effects on tested bacteria (P0.05). The highest diameter of the zone of growth inhibition was seen for L. monocytogenes (22.61±1.80 m...
Hepatitis Monthly, 2020
Background: The role of immune system in natural course of viral hepatitis has been drawn some at... more Background: The role of immune system in natural course of viral hepatitis has been drawn some attention. One of the main diagnostic markers of the immune system function in different diseases might be the ratio of CD4 + to CD8 + T lymphocytes (CD4 + /CD8 + ratio). Objectives: This research aimed to measure and compare CD4 + /CD8 + ratio in the patients with chronic hepatitis B (CHB) and control group. Methods: In this cross-sectional study, thirty-three CHB patients and thirty age and sex-matched healthy controls were included. Immunophenotyping of isolated T cells was performed using specific anti-CD4 and anti-CD8 antibodies by flow cytometry. Consequently, CD4 + , CD8 + and the ratio of CD4 + /CD8 + were counted and compared between the two groups. Results: CD4 + counts (%) were considerably reduced in patients with CHB compared to the healthy controls (51.22 ± 10.5 vs. 63.14 ± 9.9, P = 0.00), whereas CD8 + counts (%) were higher in the patients with CHB than healthy controls (48.8 ± 10.5 vs. 36.85 ± 9.86, P = 0.00). Moreover, CD4 + /CD8 + ratio remarkably decreased in the patients with CHB (1.15 ± 0.5) than healthy controls (1.93 ± 0.9) (P = 0.00). Area under curve (AUC) of 0.79 (SE = 0.06, CI = 0.68-0.90, P value = 0.05) was reported for CD4 + /CD8 + ratio with a sensitivity of 72.73% and specificity of 73.33% in 1.35 cutoff (likelihood ratio = 2.72). Conclusions: The research indicated an impaired balance between T cell subsets associated with a higher proportion of CD8 + T cells and a lower proportion of CD4 + T cells and CD4 + /CD8 + ratio in patients with CHB.
Nova Biologica Reperta, 2014
Methods of physical and chemical adsorption of heavy metals have disadvantages in some ways-such ... more Methods of physical and chemical adsorption of heavy metals have disadvantages in some ways-such as high cost and ineffectiveness at low concentrations. In recent decades methods of biological uptake of heavy metals have been investigated. The biological adsorbents include bacteria, fungi and alga, among which algae have the highest efficiency of metal uptake. This study indicated that Alginic acid is most responsible for the uptake of metals, such as nickel (II) and cadmium (II). Fucus serratus, brown alga, used as a cost-effective adsorbent for the biological uptake of cadmium and nickel ions simultaneously in a batch reactor in this study. Surface structure of algae has also been investigated. Adsorption kinetics have been measured and the results have indicated that the equilibrium time is about 300 minutes. The adsorption isotherm was interpreted by means of the Langmuir equation. The maximum adsorption rate for cadmium (II) and nickel (II) turned out to be about 0.85 and 0.95 mmol/g, respectively.
Industrial Biotechnology, 2019
Abstract The stability of enzymes produced by halophilic bacteria under harsh conditions makes th... more Abstract The stability of enzymes produced by halophilic bacteria under harsh conditions makes them appropriate candidates as biocatalysts. The present study focuses on hydrolysis enzymes productio...
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Papers by salman ahmady asbchin