Papers by saeed bagherikia
Journal of Crop Breeding, Oct 10, 2020
Environmental Stresses in Crop Sciences, 2021
Introduction The existence of drought stress at the beginning of the growing season is one of the... more Introduction The existence of drought stress at the beginning of the growing season is one of the most important threatening factors in wheat production of Iran. The coleoptile length is the most important morphological trait in determining sowing depth, emergence power and seedling establishment. The coleoptile length has been used as an effective indicator for selecting the drought tolerant genotypes in wheat breeding programs. Various indices have been developed to evaluate crop response to various stresses, including tolerance index (TOL), productivity mean (MP), geometric mean productivity (GMP), harmonic mean (HM), stress tolerance index (STI), stress sensitivity index (SSI), yield index (YI), yield stability index (YSI) and relative stress index (RSI). The efficiency of each indices depends on the breeding objectives and the target environment. Materials and methods In order to evaluate some of the landrace wheat genotypes under drought stress conditions at the seedling stage...
Physiology and Molecular Biology of Plants, 2020
The aim of this study was to investigate soil lead pollution on biochemical properties and gene e... more The aim of this study was to investigate soil lead pollution on biochemical properties and gene expression pattern of antioxidant enzymes in three wheat cultivars (Morvarid, Gonbad and Tirgan) at flag leaf sheath swollen stage. Lead (Pb(NO3)2) was used at four different concentrations (0, 15, 30 and 45 mg/kg of soil). The leaf and roots samples were taken at late-booting stage (Zadoks code, GS: 45). The results showed that lead heavy metal toxicity increased the expression of some genes and the activity of key enzymes of the antioxidant defense system in wheat. Moreover, the cell oxidation levels (MDA, LOX) enhanced under lead stress conditions. The relative gene expression and activity of antioxidant enzymes (CAT, SOD, GPX and APX) increased significantly in the both leaves and root tissues under lead stress conditions. The level of gene expression and enzymatic activity were higher in the root than the leaf tissue. There was no significant difference among cultivars in each of lea...
Journal of Plant Growth Regulation, 2018
Maintaining photosynthetic performance and remobilization of assimilates stored in vegetative tis... more Maintaining photosynthetic performance and remobilization of assimilates stored in vegetative tissues are strategies of superior wheat genotypes under drought stress conditions. To better understand the response of vegetative tissues to drought stress at the grain filling period, transcript profiling of genes encoding fructan and sucrose metabolism were studied in the stem (penultimate internode) and root of two drought-tolerant genotypes. Based on a preliminary screening, the cultivars T-65-7-1 (the mutant line) and Tabasi (wild type) were selected for further study with respect to the parameters associated with photosynthesis and stem remobilization under rain-fed conditions. The expression of photosynthetic genes, chlorophyll content and relative water content were sharply reduced in the T-65-7-1 compared to Tabasi, as a result of drought-induced leaf senescence. Under drought stress, fructan remobilization in the stem and root of T-65-7-1 was significantly higher than Tabasi, wh...
Journal of Computational Biology, 2020
The aim of this study was to determine the haplotype groups and their relationship with phenotypi... more The aim of this study was to determine the haplotype groups and their relationship with phenotypic traits in order to obtain the alleles associated with desirable agronomic characteristics in bread wheat. For this purpose, 34 local bread wheat genotypes belong to the Middle East region along with Chinese Spring variety (reference genotype) were cultivated in the form of augmented design and evaluated based on their 12 phenotypic traits. The results of descriptive statistics showed that awn length and day to flowering had the highest and lowest phenotypic coefficient of variation respectively. Ten microsatellite markers were used to investigate the haplotype variation of QTLs associated with phenotypic traits located on wheat chromosomes 4B. The mean of polymorphic alleles, polymorphic information content (PIC) and resolving power (RP) were 4.5, 0.29 and 1.58, respectively. The genotypes were classified into 21 haplotype groups according to the allelic comparison with the reference g...
Journal of Plant Process and Function, 2018
ABSTRACT: Salinity and drought stresses are common environmental factors which reduce germination... more ABSTRACT: Salinity and drought stresses are common environmental factors which reduce germination and growth of plants. This experiment was conducted to study the effects of salinity and drought stress on seed germination and seedling growth of mugwort (Artemisia vulgaris L.). The salinity and drought treatments were applied by NaCl and PEG-6000, respectively. Different concentration of NaCl (0 to 100 mM) and PEG-6000 (0 to -0.25 Mpa) were used for seed treatment. The purpose of the present research was to survey the influence of salt and water stresses on seed germination, seedling growth, fresh and dry weight in mugwort.
Journal of Crop Breeding, Sep 1, 2018
Water deficient stress is one of the major limiting factors of wheat production in arid and semia... more Water deficient stress is one of the major limiting factors of wheat production in arid and semiarid areas of the world such as Iran. Under water deficient stress photosynthesis is limited and remobilization of assimilates stored in stems is more important to grain filling. In order to molecular analysis of fructan remobilization in wheat stem (penultimate) under terminal water deficit stress, T-65-7-1 mutant line (M 7 , the results of gamma irradiation) along with its wild type (cv. Tabasi), in terms of relative gene expression involved in biosynthesis and hydrolysis of fructan and transport of sucrose were studied. Water deficit treatment (30-40% field capacity) initiated at full heading stage (Zadoks 60) and sampling was conducted at two stages (7 and 21 days post anthesis). In the T-65-7-1 mutant line, a significant increase was observed in expression levels of genes involved in fructan biosynthesis (1-SST and 6-SFT), fructan biosynthesis (6-FEH) as well as sucrose transport (SUT1 and SUT2) compared to the wild type (cv. Tabasi). This event is due to more remobilization in T-65-7-1 mutant compared to the wild type. In T-65-7-1 mutant, during early grain filling period, 1-SST and 6-SFT produces more storage of fructan in the stem. Also, during rapid grain filling stage, 6-FEH produces more sucrose and finally, SUT1 and SUT2 transports more sucrose from stem to grain. In wheat breeding programs, 1-SST, 6-SFT, 6-FEH, SUT1 and SUT2 can be used as indicators for selecting genotypes with high fructan content and more remobilization.
Journal of Crop Breeding
Identification of the appropriate dose for mutagen material is the most important step in setting... more Identification of the appropriate dose for mutagen material is the most important step in setting experiment and creating mutant genetic material. According to a definition the most appropriate dose of mutagen is a dose that causing a reduction of 50% survival or 30% growth, compared to control. The aim of this study was to evaluate the effect of different doses of gamma radiation (0 as a control, 100, 200, 300 and 400 Gary) on the early growth characteristics for determining the appropriate dose of gamma irradiation in wheat cultivar Sardari. Therefore, an experiment was conducted based on completely randomized design with three replications at the laboratory and greenhouse. Data analysis of seed germination experiment showed significant difference in all traits including rootlet length, stemlet length, fresh weight of rootlet, fresh weight of stemlet, dry weight of rootlet and dry weight of stemlet, the exception of germination rate. Analysis of data obtained from a greenhouse, also showed significant difference in plant height and survival percentage. Based on the best fitted regression model and probit analysis determined a dose causing reduction of 50% survival or 30% growth, compared to control. It was in the range between 200 to 300 Gary, for Sardari cultivar. It could help to create adequate genetic diversity by applying this range of gamma radiation, for using in the wheat mutation breeding programs.
Biologia
Sketching an appropriate genotyping strategy needs a comprehensive understanding of plant enduran... more Sketching an appropriate genotyping strategy needs a comprehensive understanding of plant endurance under stress. This study was performed to investigate the physiological and molecular responses of two rice genotypes (Hashemi parental and Hashemi advanced mutant line) with different sensitivity to salinity stress. Twenty five-day-old seedlings were treated with 0 (as control), 5 and 10 dS/m of NaCl and their characteristics corresponding to antioxidant enzymes were evaluated three times including 6 h, 48 h and 1 w after salinity treatment. The results showed that salinity stress significantly enhanced the cell oxidation levels [as thiobarbituric acid reactive substances (TBARS) content and lipoxygenase (LOX) activity] as well as antioxidant enzymes activities [superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and glutathione reductase (GR)] and ascorbate content. Moreover, the relative expression of SOD , CAT , ascorbate peroxidase ( APX ) and Oryza sativa dehydration responsive element binding ( OsDREB2 ) genes significantly increased in response to salinity stress. Hashemi advanced mutant line (as tolerant genotype) showed the highest antioxidant enzymes activities and relative genes expression compared with Hashemi parental (as sensitive genotype). It can be concluded that overexpression of SOD , CAT , APX and OsDREB2 genes could be applied to enhance tolerance to salt stress.
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Papers by saeed bagherikia