Current Research on Biosciences and Biotechnology, Aug 31, 2022
Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoi... more Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoids. This study aims to develop a method of quercitrin enrichment by utilizing macroporous resin, which is known to be safer, more eco-friendly, economics, and efficient. Evaluations were conducted over the performance and separation characteristics of the macroporous resin in quercitrin enrichment as well as the adsorption and desorption of quercitrin by the macroporous resin. The results showed that the adsorption process of the macroporous resin in relation to the amount of quercitrin in the extract were in accordance with the second order model, which means that the process of adsorption is affected by other compounds. Furthermore, the examination of the isotherm adsorption fit the Freundlich's model (R 2 = 0.9850) rather than the Langmuir's one (R 2 = 0.4334). In the optimal condition, the enrichment of quercitrin by using macroporous resin increased the abundance of quercitrin by nearly five times, from 3.60% of quercitrin content in the extract to 17.02% in the quercitrinrich fraction, with recovery yield of 50.39%.
Tofu is a food product that is easily contaminated by microbial due to its water content. Some ba... more Tofu is a food product that is easily contaminated by microbial due to its water content. Some bacteria that usually grow in tofu are Escherichia coli, Bacillus cereus, or Staphylococcus aureus. Preservatives are added to solve the common storage problem. However, some manufacturers use hazardous substances, such as formalin or other chemical substances, as a preservative. Tiwai onion (Sisyrinchium palmifolium L.) is a plant that grows in Borneo and has a broad range of antibacterial activity. This study aimed to examine the activity and effectiveness of Sisyrinchium palmifolium extract as a preservative in tofu. Sisyrinchium palmifolium was extracted using the maceration method with ethanol three times. The concentrated ethanol extract has a 5% water content and was used for the next step. According to the Clinical and Laboratory Standard Institute (CLSI method), the test was done with agar diffusion and broth microdilution methods. The test solution was diluted using DMSO 5% as the solvent, and tetracycline HCl solution as a positive control was diluted using NaCl 0.9%. Agar diffusion method was done with Sisyrinchium palmifolium ethanol extract 10000 μg/mL and tetracycline HCl 50 μg/mL. The microdilution method was done with Sisyrinchium palmifolium ethanol extract with an initial concentration of 40000 μg/mL and tetracycline HCl 2000 μg/mL. The results showed that Sisyrinchium palmifolium ethanolic extract has antibacterial activity with the minimum inhibitory concentration value of 5000 μg / mL. Then, the effectivity of concentrated ethanol extract of Sisyrinchium palmifolium as a preservative in tofu was tested by determining Total Plate Count at an incubation temperature of 37, 25, and 4 °C in comparison to potassium
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
International Journal of Applied Pharmaceutics, Jul 7, 2023
Objective: This study aims to develop and evaluate the phytosome of Phyllanthus emblica L. fruit ... more Objective: This study aims to develop and evaluate the phytosome of Phyllanthus emblica L. fruit extract. Methods: The formation of phytosome was performed by using three methods, namely solvent evaporation, antisolvent, and thin layer formation method. The phytosome was evaluated for its entrapment efficiency, particle size, polydispersity index, Fourier Transform Infra-Red (FTIR), and Transmission Electron Microscope (TEM) methods. The phytosome was made into cream with a concentration of 1%. The cream stability was evaluated at a room temperature of 40 °C for 28 d. The permeation of the total phenolic content of cream-containing phytosome and creamcontaining extract without phytosome technology was evaluated by diffusion studies. Results: The best method to prepare phytosome was an antisolvent method with the efficiency of entrapment, particle size, and polydispersity index equal to 66.99±0.01%, 298.53 nm±12.04, and 0.323±0.01 respectively. Evaluation by using TEM and FTIR spectroscopy also confirmed the formation of spherical phytosome. There were no significant changes in the cream's organoleptic, pH, and viscosity during storage at room temperature. The permeation test through skin snake and Spangler's membrane showed that cream containing phytosome had a better diffusion percentage than cream containing extract without phytosome technology. Conclusion: The results of this study showed that the antisolvent method was a potential method to develop phytosome of Phyllanthus emblica L. fruit extract and cream containing phytosome was effective in increasing the permeation.
Eleutherine americana has been shown to have many biological activities such as anti-inflammatory... more Eleutherine americana has been shown to have many biological activities such as anti-inflammatory, antipyretic, antiproliferation, antibiotic, and antivirus activities. Many chemical constituents of E. americana have also been identified, of which several may be responsible for these biological activities. In this study, we investigated the potential for these compounds to be developed as anti-inflammatory and antiviral agents through interaction study using molecular docking simulations and in silico toxicity predictions. Four protein target molecules were used in the study; cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) were used to screen potential compounds for antiinflammatory activity and polymerase acidic protein N-terminal domain (PAN) and polymerase basic protein 2 (PB2) were used to screen for antiviral activity. One compound, β-sitosterol, was shown to have a high affinity for COX-1, COX-2, and PB2; β-sitosterol returned a lower binding free energy score than celecoxib, and a binding free energy score on par with pimodivir. Another compound, compound 9, was also identified as having a higher affinity for PAN than baloxavir. Toxicity predictions of both of these compounds showed none to low toxicity, and thus these compounds have the potential to be developed further as anti-inflammatory and antiviral agents.
Research journal of pharmacy and technology, May 31, 2023
Artocarpus is a genus of Moraceae which is scientifically proven to have pharmacological activiti... more Artocarpus is a genus of Moraceae which is scientifically proven to have pharmacological activities including antioxidants and antibacterials. The purpose of this article review on Artocarpus aims to determine the species of Artocarpus that have been studied and proven to have antioxidant and antibacterial activity. Body parts that have been studied include stems, bark, roots, fruit, leaves, twigs and latex. The results of this review concluded that Artocarpus has potential to be developed as an antioxidant related to diseases caused by the effect of free radicals and antibacterial to treat infectious diseases.
IOP Conference Series: Earth and Environmental Science
Bantimurung is located in the early tertiary isolated small land area in Sulawesi, and this is th... more Bantimurung is located in the early tertiary isolated small land area in Sulawesi, and this is the factor that caused the endemism of its flora and fauna. Pothos tener Wall is an excellent aquatic plant used as a bioindicator in rain tropical forests and its lives under the Bantimurung waterfalls. Aquatic plant biodiversity has a great potential for bringing about new pharmaceutical inventions derived from freshwater aquatic flora. The genus Pothos is quite popular as a medicinal plant in India and Srilanka similarly P. scandens and P.chinensis as an anti-inflammatory, anticancer, cytotoxic potential, anti-oxidant, antibacterial, anthelmintic potential, antipyretic, anti-diabetic, and bronchodilator. However, the research regarding P.tenner is quite lacking. The study aimed to analyze the antioxidant activity from three extracts (methanol, ethyl acetate, and n-hexane) of P.tenner as an endemic plant from Indonesia. The TLC chromatogram pattern of three extracts had identified the fr...
International Journal of Applied Pharmaceutics, 2021
Objective: This study was to describe the histochemical and morpho-anatomical of kabau seeds orig... more Objective: This study was to describe the histochemical and morpho-anatomical of kabau seeds originating from Lampung, Sumatra Indonesia. Methods: Microscopic anatomical analysis of kabau seeds was carried out on the parts of kabau seeds with an incision as thick as 100 μm. The sample was placed on a glass object and aquadest, glycerin and choral hydrate were added and then covered with a glass cover, then observed under the light microscope equipped with digital camera, and analysis using the S-Viewer program. Histochemical tests are carried out with cross sections, which are colored with the following: Lugol iodine solution; ferric chloride; dragendr of; ninhydrin; K2Cr2O7. Results: Macroscopic characteristics, neatly arranged cylindrical kabau seeds consisting of five to six seeds on each pod. Yellowish-white kabau seeds are covered in a black seed coat, have a distinctive odor like jengkol or jering, have a slightly bittersweetness and a soft texture. The size of kabau seeds is ...
Journal of chemical and pharmaceutical research, 2015
The objectives of this research were to study antio xidant activities from various fruit extracts... more The objectives of this research were to study antio xidant activities from various fruit extracts of th ree organs of S. edule using two methods of antioxidant assays which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and CUPR AC (Cupric ion Reducing Antioxidant Capacity) and corr elation of their total flavonoid, phenolic and caro tenoid content with IC50 of DPPH antioxidant activities and EC 50 of CUPRAC capacities. Extraction was performed by reflux apparatus using different polarity solvents. The extracts were evaporated using rotary evaporat or. Antioxidant capacities were tested using DPPH and C UPRAC assays. Determination of total phenolic, flav onoid and carotenoid content was performed by spectropho tometer UV-visible and their correlation with IC 50 of DPPH scavenging capacities and EC 50 of CUPRAC capacities were analyzed by Pearson’s me thod. Ethyl acetate pedicel extract of S. edule (PD2) had the lowest IC 50 of DPPH scavenging activity 1.3 μg/ml, while ethyl acetate fruit...
The objectives of this study were to determine antioxidative activities of waste products (peel a... more The objectives of this study were to determine antioxidative activities of waste products (peel and seed) and flesh of arumanis mango (Mangifera indica L. 'Arumanis') using 2,2-diphenyl-1-picrylhydrazyl (DPPH), Cupric Reducing Antioxidant Capacity (CUPRAC) methods and to analyze the correlation between total phenolic content (TPC) and total flavonoid content (TFC) with their antioxidative activities. TPC, TFC and antioxidative activities were observed by UV-Vis spectrophotometry. Pearson's method was used to elaborate the relationship between phytochemical content with their antioxidative activities. The ethanol seed extract gave the top antioxidative activity using DPPH and ethyl acetate flesh extract by CUPRAC assay. The highest TPC was showed by ethyl acetate seed extract, meanwhile, ethanol peel extract gave the highest TFC. The TFC in arumanis mango peel, flesh, and seed extracts had a significant and positive correlation with their AAI (antioxidant activity index) CUPRAC. In general, the ethyl acetate and ethanol extracts of waste products of arumanis mango were greatly potent antioxidants by DPPH and CUPRAC assays. Flavonoid compounds in arumanis mango peel, flesh and seed were the primary contributors in antioxidative activities by CUPRAC method. The waste products (peel and seed) of arumanis mango fruit were potential sources as natural antioxidants.
Current Research on Biosciences and Biotechnology, Aug 31, 2022
Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoi... more Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoids. This study aims to develop a method of quercitrin enrichment by utilizing macroporous resin, which is known to be safer, more eco-friendly, economics, and efficient. Evaluations were conducted over the performance and separation characteristics of the macroporous resin in quercitrin enrichment as well as the adsorption and desorption of quercitrin by the macroporous resin. The results showed that the adsorption process of the macroporous resin in relation to the amount of quercitrin in the extract were in accordance with the second order model, which means that the process of adsorption is affected by other compounds. Furthermore, the examination of the isotherm adsorption fit the Freundlich's model (R 2 = 0.9850) rather than the Langmuir's one (R 2 = 0.4334). In the optimal condition, the enrichment of quercitrin by using macroporous resin increased the abundance of quercitrin by nearly five times, from 3.60% of quercitrin content in the extract to 17.02% in the quercitrinrich fraction, with recovery yield of 50.39%.
Objective: The objective of the present study was to isolate and determine diterpene compound and... more Objective: The objective of the present study was to isolate and determine diterpene compound and essential oils from Hedychium roxburghii Blume rhizome and investigated those antimicrobial activities. Methods: The essential oils were obtained by steam distillation method, the residual was then extracted by reflux with ethanol. The content of essential oils was analyzed by gas chromatography-mass spectrometry (GC/MS) method. Ethanolic residual-distillation extract was concentrated then used to isolate compound 1 by vacuum liquid chromatography and centrifugal chromatography. It was characterized by infrared spectrophotometry, 1H-nuclear magnetic resonance (NMR), 13C-NMR, heteronuclear single quantum coherence-NMR, heteronuclear multiple bond correlation-NMR and carbon coupling13C-NMR. The antimicrobial activity of essential oils, ethanolic residual-distillation extract and compound 1 were carried out by microdilution method. Results: The oils exhibited antimicrobial activity against...
Cymbopogon is a genus of aromatic plants that famed because of the essential oil production that ... more Cymbopogon is a genus of aromatic plants that famed because of the essential oil production that has many benefit in industrial, daily life, or even for disease treatment. The Cymbopogon genus consists of about 180 plants, including different species, subspecies, varieties, and subvarieties. The aim of this review is to present chemical properties, pharmacological, and development of the Cymbopogon genus from books and research studies. There were many types of research that have been done to prove the benefits of the Cymbopogon genus, including studies about the extract, essential oil, the chemical compound biological activity, and development studies. Some pharmacological studies that have been confirmed are regarding the pharmacological effects of Cymbopogon in the central nervous system, anti-inflammatory, antimicrobial, antioxidant, and anticancer activity. In addition, there have been studied to improve Cymbopogon benefits.
Open Access Macedonian Journal of Medical Sciences
BACKGROUND OF THE STUDY: Antioxidants are able to fight against free radicals which then prevent ... more BACKGROUND OF THE STUDY: Antioxidants are able to fight against free radicals which then prevent degenerative diseases. Antioxidants can be found in many plants such as water apples. AIM OF THE STUDY: This research is aimed to determine the antioxidant activity of green honey deli water apple (Syzygium aqueum) leaves, branches, fruits extracts through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Cupric Reducing Antioxidant Capacity (CUPRAC) methods, total phenolic content (TPC), total flavonoid content (TFC), correlation of TPC and TFC on antioxidant activity, correlation between DPPH and CUPRAC methods, and content of flavonoid compounds found in ethanol fruit extract of green honey deli water apple. METHODOLOGY: Antioxidant activities were examined by determining ascorbic acid equivalent (AAE) through DPPH and CUPRAC methods. TPC and TFC were determined using UV-vis spectrophotometry. Correlation of TPC and TFC on antioxidant activity and correlation between DPPH and CUPRAC results we...
Objective: The objective of the present study were to isolate and determine diterpene compound an... more Objective: The objective of the present study were to isolate and determine diterpene compound and essential oils from H. roxburghii Bl. rhizome, and investigated those antimicrobial activity.Methods: The essential oils was obtained by steam distillation method, the residual was then extracted by reflux with ethanol. The content of essential oils was analyzed by GC/MS method. Ethanolic residual-distillation extract was concentrated then used to isolate compound 1 by vacuum liquid chromatography and centrifugal chromatography. It was characterized by infrared spectrophotometry, 1H-NMR, 13C-NMR, HSQC-NMR, HMBC-NMR and carbon coupling NMR. The antimicrobial activity of essential oils, ethanolic residual-distillation extract and compound 1 were carried out by microdilution method.Results: The oils exhibited antimicrobial activity against Bacillus subtilis ATCC 6633 (MIC 1,750 μg/ml), Staphylococcus aureus ATCC 6538 (MIC 1,750 μg/ml), Escherichia coli ATCC 8939 (MIC 3,500 μg/ml), Ps...
The objectives of this research were to study antioxidant capacity from various leaves extracts o... more The objectives of this research were to study antioxidant capacity from various leaves extracts of legumes using two methods of antioxidant testing which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) and correlation of total flavonoid, phenolic and carotenoid content in various leaves extracts of legumes with IC50 of DPPH and EC50 of FRAP antioxidant capacities. Extraction was performed by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant capacities using DPPH and FRAP assays, determination of total phenolic, flavonoid and carotenoid content were performed by spectrophotometry UVvisible and its correlation with IC50 of DPPH scavenging capacities and EC50 of FRAP capacities were analyzed by Pearson’s method. Ethanolic leaves extract of green bean (GB3) had the lowest IC50 of DPPH scavenging capacity with IC50 1.9 g/ml and the lowest EC50 of FRAP capacity with EC50 46.9 g/ml. Ethyl ace...
Current Research on Biosciences and Biotechnology, Aug 31, 2022
Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoi... more Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoids. This study aims to develop a method of quercitrin enrichment by utilizing macroporous resin, which is known to be safer, more eco-friendly, economics, and efficient. Evaluations were conducted over the performance and separation characteristics of the macroporous resin in quercitrin enrichment as well as the adsorption and desorption of quercitrin by the macroporous resin. The results showed that the adsorption process of the macroporous resin in relation to the amount of quercitrin in the extract were in accordance with the second order model, which means that the process of adsorption is affected by other compounds. Furthermore, the examination of the isotherm adsorption fit the Freundlich's model (R 2 = 0.9850) rather than the Langmuir's one (R 2 = 0.4334). In the optimal condition, the enrichment of quercitrin by using macroporous resin increased the abundance of quercitrin by nearly five times, from 3.60% of quercitrin content in the extract to 17.02% in the quercitrinrich fraction, with recovery yield of 50.39%.
Tofu is a food product that is easily contaminated by microbial due to its water content. Some ba... more Tofu is a food product that is easily contaminated by microbial due to its water content. Some bacteria that usually grow in tofu are Escherichia coli, Bacillus cereus, or Staphylococcus aureus. Preservatives are added to solve the common storage problem. However, some manufacturers use hazardous substances, such as formalin or other chemical substances, as a preservative. Tiwai onion (Sisyrinchium palmifolium L.) is a plant that grows in Borneo and has a broad range of antibacterial activity. This study aimed to examine the activity and effectiveness of Sisyrinchium palmifolium extract as a preservative in tofu. Sisyrinchium palmifolium was extracted using the maceration method with ethanol three times. The concentrated ethanol extract has a 5% water content and was used for the next step. According to the Clinical and Laboratory Standard Institute (CLSI method), the test was done with agar diffusion and broth microdilution methods. The test solution was diluted using DMSO 5% as the solvent, and tetracycline HCl solution as a positive control was diluted using NaCl 0.9%. Agar diffusion method was done with Sisyrinchium palmifolium ethanol extract 10000 μg/mL and tetracycline HCl 50 μg/mL. The microdilution method was done with Sisyrinchium palmifolium ethanol extract with an initial concentration of 40000 μg/mL and tetracycline HCl 2000 μg/mL. The results showed that Sisyrinchium palmifolium ethanolic extract has antibacterial activity with the minimum inhibitory concentration value of 5000 μg / mL. Then, the effectivity of concentrated ethanol extract of Sisyrinchium palmifolium as a preservative in tofu was tested by determining Total Plate Count at an incubation temperature of 37, 25, and 4 °C in comparison to potassium
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
International Journal of Applied Pharmaceutics, Jul 7, 2023
Objective: This study aims to develop and evaluate the phytosome of Phyllanthus emblica L. fruit ... more Objective: This study aims to develop and evaluate the phytosome of Phyllanthus emblica L. fruit extract. Methods: The formation of phytosome was performed by using three methods, namely solvent evaporation, antisolvent, and thin layer formation method. The phytosome was evaluated for its entrapment efficiency, particle size, polydispersity index, Fourier Transform Infra-Red (FTIR), and Transmission Electron Microscope (TEM) methods. The phytosome was made into cream with a concentration of 1%. The cream stability was evaluated at a room temperature of 40 °C for 28 d. The permeation of the total phenolic content of cream-containing phytosome and creamcontaining extract without phytosome technology was evaluated by diffusion studies. Results: The best method to prepare phytosome was an antisolvent method with the efficiency of entrapment, particle size, and polydispersity index equal to 66.99±0.01%, 298.53 nm±12.04, and 0.323±0.01 respectively. Evaluation by using TEM and FTIR spectroscopy also confirmed the formation of spherical phytosome. There were no significant changes in the cream's organoleptic, pH, and viscosity during storage at room temperature. The permeation test through skin snake and Spangler's membrane showed that cream containing phytosome had a better diffusion percentage than cream containing extract without phytosome technology. Conclusion: The results of this study showed that the antisolvent method was a potential method to develop phytosome of Phyllanthus emblica L. fruit extract and cream containing phytosome was effective in increasing the permeation.
Eleutherine americana has been shown to have many biological activities such as anti-inflammatory... more Eleutherine americana has been shown to have many biological activities such as anti-inflammatory, antipyretic, antiproliferation, antibiotic, and antivirus activities. Many chemical constituents of E. americana have also been identified, of which several may be responsible for these biological activities. In this study, we investigated the potential for these compounds to be developed as anti-inflammatory and antiviral agents through interaction study using molecular docking simulations and in silico toxicity predictions. Four protein target molecules were used in the study; cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) were used to screen potential compounds for antiinflammatory activity and polymerase acidic protein N-terminal domain (PAN) and polymerase basic protein 2 (PB2) were used to screen for antiviral activity. One compound, β-sitosterol, was shown to have a high affinity for COX-1, COX-2, and PB2; β-sitosterol returned a lower binding free energy score than celecoxib, and a binding free energy score on par with pimodivir. Another compound, compound 9, was also identified as having a higher affinity for PAN than baloxavir. Toxicity predictions of both of these compounds showed none to low toxicity, and thus these compounds have the potential to be developed further as anti-inflammatory and antiviral agents.
Research journal of pharmacy and technology, May 31, 2023
Artocarpus is a genus of Moraceae which is scientifically proven to have pharmacological activiti... more Artocarpus is a genus of Moraceae which is scientifically proven to have pharmacological activities including antioxidants and antibacterials. The purpose of this article review on Artocarpus aims to determine the species of Artocarpus that have been studied and proven to have antioxidant and antibacterial activity. Body parts that have been studied include stems, bark, roots, fruit, leaves, twigs and latex. The results of this review concluded that Artocarpus has potential to be developed as an antioxidant related to diseases caused by the effect of free radicals and antibacterial to treat infectious diseases.
IOP Conference Series: Earth and Environmental Science
Bantimurung is located in the early tertiary isolated small land area in Sulawesi, and this is th... more Bantimurung is located in the early tertiary isolated small land area in Sulawesi, and this is the factor that caused the endemism of its flora and fauna. Pothos tener Wall is an excellent aquatic plant used as a bioindicator in rain tropical forests and its lives under the Bantimurung waterfalls. Aquatic plant biodiversity has a great potential for bringing about new pharmaceutical inventions derived from freshwater aquatic flora. The genus Pothos is quite popular as a medicinal plant in India and Srilanka similarly P. scandens and P.chinensis as an anti-inflammatory, anticancer, cytotoxic potential, anti-oxidant, antibacterial, anthelmintic potential, antipyretic, anti-diabetic, and bronchodilator. However, the research regarding P.tenner is quite lacking. The study aimed to analyze the antioxidant activity from three extracts (methanol, ethyl acetate, and n-hexane) of P.tenner as an endemic plant from Indonesia. The TLC chromatogram pattern of three extracts had identified the fr...
International Journal of Applied Pharmaceutics, 2021
Objective: This study was to describe the histochemical and morpho-anatomical of kabau seeds orig... more Objective: This study was to describe the histochemical and morpho-anatomical of kabau seeds originating from Lampung, Sumatra Indonesia. Methods: Microscopic anatomical analysis of kabau seeds was carried out on the parts of kabau seeds with an incision as thick as 100 μm. The sample was placed on a glass object and aquadest, glycerin and choral hydrate were added and then covered with a glass cover, then observed under the light microscope equipped with digital camera, and analysis using the S-Viewer program. Histochemical tests are carried out with cross sections, which are colored with the following: Lugol iodine solution; ferric chloride; dragendr of; ninhydrin; K2Cr2O7. Results: Macroscopic characteristics, neatly arranged cylindrical kabau seeds consisting of five to six seeds on each pod. Yellowish-white kabau seeds are covered in a black seed coat, have a distinctive odor like jengkol or jering, have a slightly bittersweetness and a soft texture. The size of kabau seeds is ...
Journal of chemical and pharmaceutical research, 2015
The objectives of this research were to study antio xidant activities from various fruit extracts... more The objectives of this research were to study antio xidant activities from various fruit extracts of th ree organs of S. edule using two methods of antioxidant assays which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and CUPR AC (Cupric ion Reducing Antioxidant Capacity) and corr elation of their total flavonoid, phenolic and caro tenoid content with IC50 of DPPH antioxidant activities and EC 50 of CUPRAC capacities. Extraction was performed by reflux apparatus using different polarity solvents. The extracts were evaporated using rotary evaporat or. Antioxidant capacities were tested using DPPH and C UPRAC assays. Determination of total phenolic, flav onoid and carotenoid content was performed by spectropho tometer UV-visible and their correlation with IC 50 of DPPH scavenging capacities and EC 50 of CUPRAC capacities were analyzed by Pearson’s me thod. Ethyl acetate pedicel extract of S. edule (PD2) had the lowest IC 50 of DPPH scavenging activity 1.3 μg/ml, while ethyl acetate fruit...
The objectives of this study were to determine antioxidative activities of waste products (peel a... more The objectives of this study were to determine antioxidative activities of waste products (peel and seed) and flesh of arumanis mango (Mangifera indica L. 'Arumanis') using 2,2-diphenyl-1-picrylhydrazyl (DPPH), Cupric Reducing Antioxidant Capacity (CUPRAC) methods and to analyze the correlation between total phenolic content (TPC) and total flavonoid content (TFC) with their antioxidative activities. TPC, TFC and antioxidative activities were observed by UV-Vis spectrophotometry. Pearson's method was used to elaborate the relationship between phytochemical content with their antioxidative activities. The ethanol seed extract gave the top antioxidative activity using DPPH and ethyl acetate flesh extract by CUPRAC assay. The highest TPC was showed by ethyl acetate seed extract, meanwhile, ethanol peel extract gave the highest TFC. The TFC in arumanis mango peel, flesh, and seed extracts had a significant and positive correlation with their AAI (antioxidant activity index) CUPRAC. In general, the ethyl acetate and ethanol extracts of waste products of arumanis mango were greatly potent antioxidants by DPPH and CUPRAC assays. Flavonoid compounds in arumanis mango peel, flesh and seed were the primary contributors in antioxidative activities by CUPRAC method. The waste products (peel and seed) of arumanis mango fruit were potential sources as natural antioxidants.
Current Research on Biosciences and Biotechnology, Aug 31, 2022
Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoi... more Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoids. This study aims to develop a method of quercitrin enrichment by utilizing macroporous resin, which is known to be safer, more eco-friendly, economics, and efficient. Evaluations were conducted over the performance and separation characteristics of the macroporous resin in quercitrin enrichment as well as the adsorption and desorption of quercitrin by the macroporous resin. The results showed that the adsorption process of the macroporous resin in relation to the amount of quercitrin in the extract were in accordance with the second order model, which means that the process of adsorption is affected by other compounds. Furthermore, the examination of the isotherm adsorption fit the Freundlich's model (R 2 = 0.9850) rather than the Langmuir's one (R 2 = 0.4334). In the optimal condition, the enrichment of quercitrin by using macroporous resin increased the abundance of quercitrin by nearly five times, from 3.60% of quercitrin content in the extract to 17.02% in the quercitrinrich fraction, with recovery yield of 50.39%.
Objective: The objective of the present study was to isolate and determine diterpene compound and... more Objective: The objective of the present study was to isolate and determine diterpene compound and essential oils from Hedychium roxburghii Blume rhizome and investigated those antimicrobial activities. Methods: The essential oils were obtained by steam distillation method, the residual was then extracted by reflux with ethanol. The content of essential oils was analyzed by gas chromatography-mass spectrometry (GC/MS) method. Ethanolic residual-distillation extract was concentrated then used to isolate compound 1 by vacuum liquid chromatography and centrifugal chromatography. It was characterized by infrared spectrophotometry, 1H-nuclear magnetic resonance (NMR), 13C-NMR, heteronuclear single quantum coherence-NMR, heteronuclear multiple bond correlation-NMR and carbon coupling13C-NMR. The antimicrobial activity of essential oils, ethanolic residual-distillation extract and compound 1 were carried out by microdilution method. Results: The oils exhibited antimicrobial activity against...
Cymbopogon is a genus of aromatic plants that famed because of the essential oil production that ... more Cymbopogon is a genus of aromatic plants that famed because of the essential oil production that has many benefit in industrial, daily life, or even for disease treatment. The Cymbopogon genus consists of about 180 plants, including different species, subspecies, varieties, and subvarieties. The aim of this review is to present chemical properties, pharmacological, and development of the Cymbopogon genus from books and research studies. There were many types of research that have been done to prove the benefits of the Cymbopogon genus, including studies about the extract, essential oil, the chemical compound biological activity, and development studies. Some pharmacological studies that have been confirmed are regarding the pharmacological effects of Cymbopogon in the central nervous system, anti-inflammatory, antimicrobial, antioxidant, and anticancer activity. In addition, there have been studied to improve Cymbopogon benefits.
Open Access Macedonian Journal of Medical Sciences
BACKGROUND OF THE STUDY: Antioxidants are able to fight against free radicals which then prevent ... more BACKGROUND OF THE STUDY: Antioxidants are able to fight against free radicals which then prevent degenerative diseases. Antioxidants can be found in many plants such as water apples. AIM OF THE STUDY: This research is aimed to determine the antioxidant activity of green honey deli water apple (Syzygium aqueum) leaves, branches, fruits extracts through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Cupric Reducing Antioxidant Capacity (CUPRAC) methods, total phenolic content (TPC), total flavonoid content (TFC), correlation of TPC and TFC on antioxidant activity, correlation between DPPH and CUPRAC methods, and content of flavonoid compounds found in ethanol fruit extract of green honey deli water apple. METHODOLOGY: Antioxidant activities were examined by determining ascorbic acid equivalent (AAE) through DPPH and CUPRAC methods. TPC and TFC were determined using UV-vis spectrophotometry. Correlation of TPC and TFC on antioxidant activity and correlation between DPPH and CUPRAC results we...
Objective: The objective of the present study were to isolate and determine diterpene compound an... more Objective: The objective of the present study were to isolate and determine diterpene compound and essential oils from H. roxburghii Bl. rhizome, and investigated those antimicrobial activity.Methods: The essential oils was obtained by steam distillation method, the residual was then extracted by reflux with ethanol. The content of essential oils was analyzed by GC/MS method. Ethanolic residual-distillation extract was concentrated then used to isolate compound 1 by vacuum liquid chromatography and centrifugal chromatography. It was characterized by infrared spectrophotometry, 1H-NMR, 13C-NMR, HSQC-NMR, HMBC-NMR and carbon coupling NMR. The antimicrobial activity of essential oils, ethanolic residual-distillation extract and compound 1 were carried out by microdilution method.Results: The oils exhibited antimicrobial activity against Bacillus subtilis ATCC 6633 (MIC 1,750 μg/ml), Staphylococcus aureus ATCC 6538 (MIC 1,750 μg/ml), Escherichia coli ATCC 8939 (MIC 3,500 μg/ml), Ps...
The objectives of this research were to study antioxidant capacity from various leaves extracts o... more The objectives of this research were to study antioxidant capacity from various leaves extracts of legumes using two methods of antioxidant testing which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) and correlation of total flavonoid, phenolic and carotenoid content in various leaves extracts of legumes with IC50 of DPPH and EC50 of FRAP antioxidant capacities. Extraction was performed by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant capacities using DPPH and FRAP assays, determination of total phenolic, flavonoid and carotenoid content were performed by spectrophotometry UVvisible and its correlation with IC50 of DPPH scavenging capacities and EC50 of FRAP capacities were analyzed by Pearson’s method. Ethanolic leaves extract of green bean (GB3) had the lowest IC50 of DPPH scavenging capacity with IC50 1.9 g/ml and the lowest EC50 of FRAP capacity with EC50 46.9 g/ml. Ethyl ace...
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Papers by rika hartati