Iranian journal of pediatric hematology and oncology, 2013
Several factors may cause infertility and fetal loss. Blood groups antigens seem to be implied in... more Several factors may cause infertility and fetal loss. Blood groups antigens seem to be implied in the mechanisms of infertility and fetal loss. Maternal natural antibody can react against father's blood group antigens on spermatozoa. The effects of parental blood group system on infertility and fetal surveillance perceived by its manifestation in prezygotic (caused infertility) and postzygotic (caused fetal loss) stages. Objective of the present study is to determine the effect of parental ABO blood group on fetal surveillance and men infertility. This is a retrospective, cross sectional study. Our study was carried out in fertility and infertility center of Yazd city. Blood group of 118males (group1:100 males with infertility and group 2: 18 males with abortion history in female partners) that referred to this center was evaluated based on medical document's patients. Data were analyzed with SPSS 16 software using chi-square test. The results were considered significant whe...
International Journal of Reproductive Biomedicine, 2021
Background Recurrent pregnancy loss (RPL) refers to the incidence of two or more abortions before... more Background Recurrent pregnancy loss (RPL) refers to the incidence of two or more abortions before the first half of pregnancy. Oxidative stress has been hypothesized to play a central role in RPL. Objective To investigate the relationship between Q192R and L55M polymorphisms of PON1 as antioxidant enzyme and the risk of RPL. Materials and Methods In this case–control study, 110 women with RPL (case) and 110 healthy fertile women (control) referred to the Research and Clinical Center for Infertility, Shiraz, Iran were enrolled. Genomic DNA was extracted from the peripheral blood in all participants. Polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Results Statistical analysis of Q192R polymorphism showed a significant difference for the RR genotype between the case and control group (OR = 11, CI = 1.39–86.87, p = 0.005) but none for the QR and QQ genotypes. No significant association was observed between the R and Q allelic fr...
Effectiveness of aspirin compare with heparin plus aspirin in recurrent pregnancy loss treatment:... more Effectiveness of aspirin compare with heparin plus aspirin in recurrent pregnancy loss treatment: A Quasi experimental study
International Journal of Reproductive BioMedicine (IJRM)
Background: Premature ovarian failure (POF), is menopause occurring before the age of 40, affecti... more Background: Premature ovarian failure (POF), is menopause occurring before the age of 40, affecting 1-3% of women worldwide. The risk of POF increases with altered immunological parameters such as FAS and FASL genes, which play a fundamental role in embryogenesis and cellular homeostasis. Objective: The study aimed to investigate the potential role of FAS and FASL genes in POF pathogenesis. Materials and Methods: In this case-control study, the polymorphisms of FAS-670A/G and FASLIVS2nt_124A/G apoptotic genes were analyzed in 51 Iranian women suffering from POF, and 61 healthy controls. Isolation of DNA was done using the salting-out method, and genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method. Results: Our results revealed that homozygous FAS-670A/A and G/G, and heterozygous FAS-670A/G are not significantly different between cases and controls (p = 0.99). Also, in different genotyping models of...
Ultra-rare biallelic pathogenic variants in geranylgeranyl diphosphate synthase 1 (GGPS1) have re... more Ultra-rare biallelic pathogenic variants in geranylgeranyl diphosphate synthase 1 (GGPS1) have recently been associated with muscular dystrophy/hearing loss/ ovarian insufficiency syndrome. Here, we describe 11 affected individuals from four unpublished families with ultra-rare missense variants in GGPS1 and provide follow-up details from a previously reported family. Our cohort replicated most of the previously described clinical features of GGPS1 deficiency; however, hearing loss was present in only 46% of the individuals. This report consolidates the disease-causing role of biallelic variants in GGPS1 and demonstrates that hearing loss and ovarian insufficiency might be a variable feature of the GGPS1-associated muscular dystrophy.
International Journal of Reproductive BioMedicine (IJRM)
Background: A large proportion of cases of recurrent pregnancy loss (RPL) are associated with imm... more Background: A large proportion of cases of recurrent pregnancy loss (RPL) are associated with immunological factors. Objective: This study investigated the association between single nucleotide polymorphisms of cytotoxic T lymphocyte-associated protein (CTLA)-4 gene in women with a history of RPL compared to healthy women. Materials and Methods: A case-control study was performed on 2 groups consisting of 120 healthy women with no history of abortion and at least one delivery (control) and 120 women with a history of 2 or more primary RPLs (case). In addition, 5 mL of peripheral blood sample was taken from all subjects. The frequencies of CTLA-4 rs3087243 and rs231775 polymorphisms were assayed by restriction fragment length polymorphism polymerase chain reaction and rs5742909 using the high-resolution melting real-time polymerase chain reaction method. Results: The mean age of the women in the control and RPL groups were 30.03 ± 4.23 (range 21-37), and 28.64 ± 3.61 yr (range 20-35)...
Background and Aims: One of the neurotransmitters in the brain is Histamine which acts as several... more Background and Aims: One of the neurotransmitters in the brain is Histamine which acts as several biological mechanism regulators like inflammation, gastric acid secretion, and neuromodulation. Inactivation of Histamine occurs by histamine N-methyltransferase (HNMT) enzyme. The HNMT transfers a methyl group from S-adenosyl-L-methionine to Histamine and is the main process for the termination of neurotransmission actions of Histamine in the mammalian central nervous system. Materials and Methods: In this case, a family was referred to the genetic clinic to diagnose the cause of their disorder. The clinical form, pedigree, and questionnaire were completed for the family, and the parents gave their written consent for all tests and photographs publication. Both siblings have moderate learning and intellectual disability. Whole exome sequencing was performed and Sanger sequencing for co-segregation was used. Results: Bioinformatics analysis revealed a homozygous missense variant in HNMT...
Objective: Ovarian cancer is one of the most common gynaecological malignancies among females wor... more Objective: Ovarian cancer is one of the most common gynaecological malignancies among females worldwide. Early diagnostic of ovarian cancer is challenging, and miRNAs could serve as a potential biomarker for early detection of ovarian cancer regarding easy to detect. The present study investigated the expression pro le of miRNA-34a, miRNA-143, miRNA-212, Sox4, BCL-2, and E2f5 in the serum of participants to nd new biomarkers for early diagnosis of ovarian cancer. Materials and Methods: Five milliliters of whole blood were collected from each patient (n=30) and control (n=30), and total RNA was extracted using Ambion™ TRIzol™ Reagent. Real-time PCR methods evaluated the expression of targeted miRNAs and their targeted genes. Result: The Disease status of all ovarian cancer patients were classi ed as FIGO stage III (21) and (IV 9) according to imaging studies and surgical pathological ndings. Our results showed that expression levels of miRNA-34a (p<0.0001), miRNA-143 (p=0.028), and miRNA-212 (p<0.0001) were signi cantly decreased in patients with ovarian cancer compared to controls and expression levels of Sox4(p<0.0001), BCL-2(p<0.0001) and E2f5(p<0.0001) were signi cantly increased in patients with ovarian cancer compared to controls. In addition, Receiver Operating Characteristic (ROC) curve revealed that the expression pro le of miRNA-34a (AUC= 0.82; P <0.0001), miRNA-143 (AUC= 0.66; P = 0.026) and miRNA-212 (AUC= 0.78; P = 0.0001) could be used as potential biomarker for discriminating patients with ovarian cancer. Conclusion: In conclusion, our data showed that the expression pro le of miRNA-34a, miRNA-143, and miRNA-212 could act as a potential biomarker for diagnosing ovarian cancer patients.
International Journal of Reproductive BioMedicine (IJRM)
Recurrent pregnancy loss is a distinct disorder defined as the loss of at least 2 pregnancies bef... more Recurrent pregnancy loss is a distinct disorder defined as the loss of at least 2 pregnancies before the 20th wk of gestation. With half of the genome of the embryo belonging to the father, the integrity of the sperm genome is crucial for a successful pregnancy. Semen analysis is recommended for men in such cases to evaluate sperm concentration, morphology, vitality and motility. However, other important sperm parameters such as sperm epigenetics, aneuploidy, Y chromosome microdeletion and chromatin integrity also correlate with successful pregnancy and delivery rate. This article examines the use of different sperm tests and their importance in male partners of women suffering from recurrent pregnancy loss. Key words: DNA fragmentation, Sperm, Y chromosome, Recurrent pregnancy loss.
International Journal of Reproductive BioMedicine (IJRM)
Background: Infertility is a common problem in testicular cancer. Affected men often decide to un... more Background: Infertility is a common problem in testicular cancer. Affected men often decide to undergo sperm banking before chemo/radiotherapy. The cumulative effects of therapy can considerably reduce fertility. Objective: Testicular cancers impair fertilizing ability, even before diagnosis. This study tries to verify individual traits and semen quality in patients with testicular cancer. Materials and Methods: This observational study analyzed 190 semen of patients with testicular cancer (16 to 47 yr old) referred to the sub-fertility laboratory at the St. Mary hospital for semen banking prior to treatment carcinoma. Several aspects of their semen analyses were examined. The cases were divided into four different categories: seminoma, teratoma, mixed germ cell tumors and others. Results: The results showed that 23 cases were azoospermic, and 13 of the patients who were not azoospermic, their sperm of “normal” morphology were too few to count. Among patients that could produce sper...
International Journal of Reproductive BioMedicine (IJRM)
Background: Polycystic ovary syndrome (PCOS) is a heterogeneous disorder, which affects about 15-... more Background: Polycystic ovary syndrome (PCOS) is a heterogeneous disorder, which affects about 15-20% of women of reproductive age. The most important etiopathogenesis factor in its incidence is hyperandrogenism; over 70 candidate genes are known to be associated with this syndrome, such as the androgen receptor (AR) gene which encodes a steroid receptor and is located on the Xq11-12 chromosome. The N-terminus of exon 1 of AR contains a polymorphic trinucleotide repeat (CAG)n region that encodes glutamine tract. There are some studies showing that shorter AR CAG repeats are significantly related to enhanced AR sensitivity. Objective: This study investigated the frequency of the polymorphic expansion of the trinucleotide CAG repeats of AR in PCOS. Materials and Methods: 160 Iranian women aged 17-40 yr participated in this casecontrol study: 80 women as PCOS patients and 80 women as healthy controls according to the Rotterdam criteria. Other similar phenotype factors such as hyperandro...
Background Intellectual disability is characterized by impairments in adaptive behavior and cogni... more Background Intellectual disability is characterized by impairments in adaptive behavior and cognitive functioning manifested during the developmental period. Since disabilities are heterogeneous, variant analysis can help us confirm and accurately diagnose children with intellectual disabilities. Some papers reported that bi-allelic variants of the NSUN2 gene caused a group of neurological disorders, including non-syndromic autosomal recessive intellectual disability (NS-ARID), Dubowitz syndrome, and familial restrictive cardiomyopathy 1 (RCM1). We report on a consanguineous family with three siblings diagnosed with intellectual disability. Case presentation The 7-year-old female was referred to Ali-Asghar hospital, Zahedan, Iran, with clinical manifestations comprising moderate intellectual disability, ptosis, long face, and short stature. Chromosome banding, metabolic testing, and magnetic resonance imaging examinations revealed no abnormalities. Accordingly, other affected siblin...
International Journal of Reproductive BioMedicine (IJRM), 2021
Background: Surgery and chemotherapy are the two most common treatments for cancers, including ov... more Background: Surgery and chemotherapy are the two most common treatments for cancers, including ovarian cancer. Although most ovarian cancers occur over the age of 45 yr, it may involve younger women and affect their reproductive ability. Objective: To assess the expression of Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), Forkhead Box O1 (FOXO1), and miR-340 genes in the ovarian cancer tissues as well as ovarian cancer cell lines. Materials and Methods: In this case-control study, 30 ovarian cancer samples (with the average age of 37 ± 2.5 years) coupled with their non-tumor marginal tissue (as a control) were collected. Proliferated cell lines were treated with several concentrations of cisplatin, and the half maximal inhibitory concentration (IC50) of cisplatin was quantified by MTTassay. After RNA extraction, cDNA synthesis and qRT-PCR were done. Finally, the results were analyzed. Results: While the expression levels of miR-340 and FOXO1 genes in tumor sampl...
Objective: There are still worldwide controversial attitudes regarding embryo donation (ED) progr... more Objective: There are still worldwide controversial attitudes regarding embryo donation (ED) program. The aim of this descriptive study was to evaluate the infertile couples’ opinions regarding ED program for infertility treatment in Iran. Methods: A total of 120 infertile patients (women, n=61; men, n=59) residing in Iran were asked to fill out the questionnaires. Part I of the questionnaire form contained general demographic information. Part II contained 17 questions to reveal their knowledge and attitudes about ED program in Iran. Results: The vast majority of the subjects had formal education. One-third had complete objection toward ED. 21% and 14% of the women and men thought that ED is not permitted by their religion, respectively. Nearly half of participants believed in informing the general public about ED in mass media. In addition, majority of the participants were in favor of ED over adopting a child. Psychological counseling was strongly recommended by vast majority of r...
Iranian journal of pediatric hematology and oncology, 2013
Several factors may cause infertility and fetal loss. Blood groups antigens seem to be implied in... more Several factors may cause infertility and fetal loss. Blood groups antigens seem to be implied in the mechanisms of infertility and fetal loss. Maternal natural antibody can react against father's blood group antigens on spermatozoa. The effects of parental blood group system on infertility and fetal surveillance perceived by its manifestation in prezygotic (caused infertility) and postzygotic (caused fetal loss) stages. Objective of the present study is to determine the effect of parental ABO blood group on fetal surveillance and men infertility. This is a retrospective, cross sectional study. Our study was carried out in fertility and infertility center of Yazd city. Blood group of 118males (group1:100 males with infertility and group 2: 18 males with abortion history in female partners) that referred to this center was evaluated based on medical document's patients. Data were analyzed with SPSS 16 software using chi-square test. The results were considered significant whe...
International Journal of Reproductive Biomedicine, 2021
Background Recurrent pregnancy loss (RPL) refers to the incidence of two or more abortions before... more Background Recurrent pregnancy loss (RPL) refers to the incidence of two or more abortions before the first half of pregnancy. Oxidative stress has been hypothesized to play a central role in RPL. Objective To investigate the relationship between Q192R and L55M polymorphisms of PON1 as antioxidant enzyme and the risk of RPL. Materials and Methods In this case–control study, 110 women with RPL (case) and 110 healthy fertile women (control) referred to the Research and Clinical Center for Infertility, Shiraz, Iran were enrolled. Genomic DNA was extracted from the peripheral blood in all participants. Polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Results Statistical analysis of Q192R polymorphism showed a significant difference for the RR genotype between the case and control group (OR = 11, CI = 1.39–86.87, p = 0.005) but none for the QR and QQ genotypes. No significant association was observed between the R and Q allelic fr...
Effectiveness of aspirin compare with heparin plus aspirin in recurrent pregnancy loss treatment:... more Effectiveness of aspirin compare with heparin plus aspirin in recurrent pregnancy loss treatment: A Quasi experimental study
International Journal of Reproductive BioMedicine (IJRM)
Background: Premature ovarian failure (POF), is menopause occurring before the age of 40, affecti... more Background: Premature ovarian failure (POF), is menopause occurring before the age of 40, affecting 1-3% of women worldwide. The risk of POF increases with altered immunological parameters such as FAS and FASL genes, which play a fundamental role in embryogenesis and cellular homeostasis. Objective: The study aimed to investigate the potential role of FAS and FASL genes in POF pathogenesis. Materials and Methods: In this case-control study, the polymorphisms of FAS-670A/G and FASLIVS2nt_124A/G apoptotic genes were analyzed in 51 Iranian women suffering from POF, and 61 healthy controls. Isolation of DNA was done using the salting-out method, and genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method. Results: Our results revealed that homozygous FAS-670A/A and G/G, and heterozygous FAS-670A/G are not significantly different between cases and controls (p = 0.99). Also, in different genotyping models of...
Ultra-rare biallelic pathogenic variants in geranylgeranyl diphosphate synthase 1 (GGPS1) have re... more Ultra-rare biallelic pathogenic variants in geranylgeranyl diphosphate synthase 1 (GGPS1) have recently been associated with muscular dystrophy/hearing loss/ ovarian insufficiency syndrome. Here, we describe 11 affected individuals from four unpublished families with ultra-rare missense variants in GGPS1 and provide follow-up details from a previously reported family. Our cohort replicated most of the previously described clinical features of GGPS1 deficiency; however, hearing loss was present in only 46% of the individuals. This report consolidates the disease-causing role of biallelic variants in GGPS1 and demonstrates that hearing loss and ovarian insufficiency might be a variable feature of the GGPS1-associated muscular dystrophy.
International Journal of Reproductive BioMedicine (IJRM)
Background: A large proportion of cases of recurrent pregnancy loss (RPL) are associated with imm... more Background: A large proportion of cases of recurrent pregnancy loss (RPL) are associated with immunological factors. Objective: This study investigated the association between single nucleotide polymorphisms of cytotoxic T lymphocyte-associated protein (CTLA)-4 gene in women with a history of RPL compared to healthy women. Materials and Methods: A case-control study was performed on 2 groups consisting of 120 healthy women with no history of abortion and at least one delivery (control) and 120 women with a history of 2 or more primary RPLs (case). In addition, 5 mL of peripheral blood sample was taken from all subjects. The frequencies of CTLA-4 rs3087243 and rs231775 polymorphisms were assayed by restriction fragment length polymorphism polymerase chain reaction and rs5742909 using the high-resolution melting real-time polymerase chain reaction method. Results: The mean age of the women in the control and RPL groups were 30.03 ± 4.23 (range 21-37), and 28.64 ± 3.61 yr (range 20-35)...
Background and Aims: One of the neurotransmitters in the brain is Histamine which acts as several... more Background and Aims: One of the neurotransmitters in the brain is Histamine which acts as several biological mechanism regulators like inflammation, gastric acid secretion, and neuromodulation. Inactivation of Histamine occurs by histamine N-methyltransferase (HNMT) enzyme. The HNMT transfers a methyl group from S-adenosyl-L-methionine to Histamine and is the main process for the termination of neurotransmission actions of Histamine in the mammalian central nervous system. Materials and Methods: In this case, a family was referred to the genetic clinic to diagnose the cause of their disorder. The clinical form, pedigree, and questionnaire were completed for the family, and the parents gave their written consent for all tests and photographs publication. Both siblings have moderate learning and intellectual disability. Whole exome sequencing was performed and Sanger sequencing for co-segregation was used. Results: Bioinformatics analysis revealed a homozygous missense variant in HNMT...
Objective: Ovarian cancer is one of the most common gynaecological malignancies among females wor... more Objective: Ovarian cancer is one of the most common gynaecological malignancies among females worldwide. Early diagnostic of ovarian cancer is challenging, and miRNAs could serve as a potential biomarker for early detection of ovarian cancer regarding easy to detect. The present study investigated the expression pro le of miRNA-34a, miRNA-143, miRNA-212, Sox4, BCL-2, and E2f5 in the serum of participants to nd new biomarkers for early diagnosis of ovarian cancer. Materials and Methods: Five milliliters of whole blood were collected from each patient (n=30) and control (n=30), and total RNA was extracted using Ambion™ TRIzol™ Reagent. Real-time PCR methods evaluated the expression of targeted miRNAs and their targeted genes. Result: The Disease status of all ovarian cancer patients were classi ed as FIGO stage III (21) and (IV 9) according to imaging studies and surgical pathological ndings. Our results showed that expression levels of miRNA-34a (p<0.0001), miRNA-143 (p=0.028), and miRNA-212 (p<0.0001) were signi cantly decreased in patients with ovarian cancer compared to controls and expression levels of Sox4(p<0.0001), BCL-2(p<0.0001) and E2f5(p<0.0001) were signi cantly increased in patients with ovarian cancer compared to controls. In addition, Receiver Operating Characteristic (ROC) curve revealed that the expression pro le of miRNA-34a (AUC= 0.82; P <0.0001), miRNA-143 (AUC= 0.66; P = 0.026) and miRNA-212 (AUC= 0.78; P = 0.0001) could be used as potential biomarker for discriminating patients with ovarian cancer. Conclusion: In conclusion, our data showed that the expression pro le of miRNA-34a, miRNA-143, and miRNA-212 could act as a potential biomarker for diagnosing ovarian cancer patients.
International Journal of Reproductive BioMedicine (IJRM)
Recurrent pregnancy loss is a distinct disorder defined as the loss of at least 2 pregnancies bef... more Recurrent pregnancy loss is a distinct disorder defined as the loss of at least 2 pregnancies before the 20th wk of gestation. With half of the genome of the embryo belonging to the father, the integrity of the sperm genome is crucial for a successful pregnancy. Semen analysis is recommended for men in such cases to evaluate sperm concentration, morphology, vitality and motility. However, other important sperm parameters such as sperm epigenetics, aneuploidy, Y chromosome microdeletion and chromatin integrity also correlate with successful pregnancy and delivery rate. This article examines the use of different sperm tests and their importance in male partners of women suffering from recurrent pregnancy loss. Key words: DNA fragmentation, Sperm, Y chromosome, Recurrent pregnancy loss.
International Journal of Reproductive BioMedicine (IJRM)
Background: Infertility is a common problem in testicular cancer. Affected men often decide to un... more Background: Infertility is a common problem in testicular cancer. Affected men often decide to undergo sperm banking before chemo/radiotherapy. The cumulative effects of therapy can considerably reduce fertility. Objective: Testicular cancers impair fertilizing ability, even before diagnosis. This study tries to verify individual traits and semen quality in patients with testicular cancer. Materials and Methods: This observational study analyzed 190 semen of patients with testicular cancer (16 to 47 yr old) referred to the sub-fertility laboratory at the St. Mary hospital for semen banking prior to treatment carcinoma. Several aspects of their semen analyses were examined. The cases were divided into four different categories: seminoma, teratoma, mixed germ cell tumors and others. Results: The results showed that 23 cases were azoospermic, and 13 of the patients who were not azoospermic, their sperm of “normal” morphology were too few to count. Among patients that could produce sper...
International Journal of Reproductive BioMedicine (IJRM)
Background: Polycystic ovary syndrome (PCOS) is a heterogeneous disorder, which affects about 15-... more Background: Polycystic ovary syndrome (PCOS) is a heterogeneous disorder, which affects about 15-20% of women of reproductive age. The most important etiopathogenesis factor in its incidence is hyperandrogenism; over 70 candidate genes are known to be associated with this syndrome, such as the androgen receptor (AR) gene which encodes a steroid receptor and is located on the Xq11-12 chromosome. The N-terminus of exon 1 of AR contains a polymorphic trinucleotide repeat (CAG)n region that encodes glutamine tract. There are some studies showing that shorter AR CAG repeats are significantly related to enhanced AR sensitivity. Objective: This study investigated the frequency of the polymorphic expansion of the trinucleotide CAG repeats of AR in PCOS. Materials and Methods: 160 Iranian women aged 17-40 yr participated in this casecontrol study: 80 women as PCOS patients and 80 women as healthy controls according to the Rotterdam criteria. Other similar phenotype factors such as hyperandro...
Background Intellectual disability is characterized by impairments in adaptive behavior and cogni... more Background Intellectual disability is characterized by impairments in adaptive behavior and cognitive functioning manifested during the developmental period. Since disabilities are heterogeneous, variant analysis can help us confirm and accurately diagnose children with intellectual disabilities. Some papers reported that bi-allelic variants of the NSUN2 gene caused a group of neurological disorders, including non-syndromic autosomal recessive intellectual disability (NS-ARID), Dubowitz syndrome, and familial restrictive cardiomyopathy 1 (RCM1). We report on a consanguineous family with three siblings diagnosed with intellectual disability. Case presentation The 7-year-old female was referred to Ali-Asghar hospital, Zahedan, Iran, with clinical manifestations comprising moderate intellectual disability, ptosis, long face, and short stature. Chromosome banding, metabolic testing, and magnetic resonance imaging examinations revealed no abnormalities. Accordingly, other affected siblin...
International Journal of Reproductive BioMedicine (IJRM), 2021
Background: Surgery and chemotherapy are the two most common treatments for cancers, including ov... more Background: Surgery and chemotherapy are the two most common treatments for cancers, including ovarian cancer. Although most ovarian cancers occur over the age of 45 yr, it may involve younger women and affect their reproductive ability. Objective: To assess the expression of Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), Forkhead Box O1 (FOXO1), and miR-340 genes in the ovarian cancer tissues as well as ovarian cancer cell lines. Materials and Methods: In this case-control study, 30 ovarian cancer samples (with the average age of 37 ± 2.5 years) coupled with their non-tumor marginal tissue (as a control) were collected. Proliferated cell lines were treated with several concentrations of cisplatin, and the half maximal inhibitory concentration (IC50) of cisplatin was quantified by MTTassay. After RNA extraction, cDNA synthesis and qRT-PCR were done. Finally, the results were analyzed. Results: While the expression levels of miR-340 and FOXO1 genes in tumor sampl...
Objective: There are still worldwide controversial attitudes regarding embryo donation (ED) progr... more Objective: There are still worldwide controversial attitudes regarding embryo donation (ED) program. The aim of this descriptive study was to evaluate the infertile couples’ opinions regarding ED program for infertility treatment in Iran. Methods: A total of 120 infertile patients (women, n=61; men, n=59) residing in Iran were asked to fill out the questionnaires. Part I of the questionnaire form contained general demographic information. Part II contained 17 questions to reveal their knowledge and attitudes about ED program in Iran. Results: The vast majority of the subjects had formal education. One-third had complete objection toward ED. 21% and 14% of the women and men thought that ED is not permitted by their religion, respectively. Nearly half of participants believed in informing the general public about ED in mass media. In addition, majority of the participants were in favor of ED over adopting a child. Psychological counseling was strongly recommended by vast majority of r...
Uploads
Papers by nasrin ghasemi