Papers by mojtaba hedayati
International Pharmacy Acta, Mar 4, 2018
Introduction: An important issue in cancer therapy is the achievement of desired therapeutic resp... more Introduction: An important issue in cancer therapy is the achievement of desired therapeutic response with the least adverse effects. To achieve this goal, targeted drug delivery systems were developed. Aptamers, mainly DNA/RNA aptamers, are the attractive affinity ligands for the cancer cell surface specific antigens. Besides, microRNAs are another type of therapeutic and diagnostic oligonucleotides that have been recently studied in various cancers. miRNAs are small double stranded RNAs with important roles in cell regulatory pathways. Profile changes of miRNAs can result in cancer development. External addition of miRNAs or their elimination using antagomiR can lead to the efficient treatment of related disease. Targeted delivery of therapeutic agents to the site of action with less adverse effects is the most challenging issue in anticancer chemotherapeutic agents as well as miRNA therapy. In addition, miRNAs stability in biological systems can be improved by targeting strategy. In this study, a cancer specific aptamer (anti-nucleolin aptamer) and a functional miRNA in cell growth and proliferation (miRNAlet-7d) were used in the development of targeted nano-molecules as an efficient anti-proliferative agent for cancer cells. Methods and Results: Sequences of A1411 aptamer and miRNA let-7d were extracted from related databanks and were chemically synthesized with amine and thiol modification in the 3' terminals or with a 17 nucleotides sticky extension at 3' terminal. The sequences were conjugated covalently using SM(PEG) 2 hetero-bifunctional cross-linker or un-covalently by annealing the sticky ends. Conjugation was confirmed using polyacrylamide gel electrophoresis 15%. The serum stability of these two types of conjugates were evaluated using up to 48 h incubation of conjugates in human serum (AB +). Stability of covalent conjugate using SM(PEG) 2 linker was at least two hours more than the un-covalent one. Conclusions: Remarkable advantages of this nano-molecule were targeted and relative stable delivery of miRNA as the therapeutic agent with probable synergistic effect of two oligonucleotides of miRNA and aptamer in the proliferation inhibition of cancer cells.
ChemistrySelect, Dec 1, 2022
Lipopolysaccharide (LPS) is a component of Gram‐negative bacteria‘s outer membrane that, when rel... more Lipopolysaccharide (LPS) is a component of Gram‐negative bacteria‘s outer membrane that, when released into aqueous solutions, can have devastating consequences for human health. In this study, sulfonic acid‐functionalized mesoporous silica nanocomposites bound to polymixin‐B was synthesized and their functionality to absorb the bacterial LPS from human serum was investigated. Briefly, Pseudomonas aeruginosa was isolated from burn wounds and its LPS was extracted. Then, Mesoporous MCM‐41 nanocomposites were fabricated and functionalized with the sulfonic acid groups. Finally, nanocomposites were used to extract LPS from human serum and statistical data were analyzed using the Taguchi method. Extraction of bacterial LPS with a modified method, optimization of factors affecting the extraction of endotoxin using Taguchi method, introduction of new nano‐composites with nanometer pores and obtaining results with 96 % effectiveness in removing LPS in LAL and MTT tests were the most important findings. The obtained calibration curve was linear in the ranges of 1–300 μg L‐1 with reasonable linearity (r2&gt;0.998). The limit of detection (LOD) based on S/N=3 was 0.03 μg L‐1 for 10 mL sample volumes.
Journal of Personalized Medicine, Oct 31, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of Nursing Reports in Clinical Practice
Antimicrobial resistance (AMR) is one of the growing challenges of modern medicine, which causes ... more Antimicrobial resistance (AMR) is one of the growing challenges of modern medicine, which causes thousands of deaths world-wide annually [1, 2].
Journal of Personalized Medicine
Background: Mental and physical health are both important for overall health. Mental health inclu... more Background: Mental and physical health are both important for overall health. Mental health includes emotional, psychological, and social well-being; however, it is often difficult to monitor remotely. The objective of this scoping review is to investigate studies that focus on mental health and stress detection and monitoring using PPG-based wearable sensors. Methods: A literature review for this scoping review was conducted using the PRISMA (Preferred Reporting Items for the Systematic Reviews and Meta-analyses) framework. A total of 290 studies were found in five medical databases (PubMed, Medline, Embase, CINAHL, and Web of Science). Studies were deemed eligible if non-invasive PPG-based wearables were worn on the wrist or ear to measure vital signs of the heart (heart rate, pulse transit time, pulse waves, blood pressure, and blood volume pressure) and analyzed the data qualitatively. Results: Twenty-three studies met the inclusion criteria, with four real-life studies, eightee...
Telemedicine and e-Health
Journal of Guilan University of Medical Sciences
Background: In women of childbearing age, Polycystic Ovary Syndrome (PCOS) is a common endocrine ... more Background: In women of childbearing age, Polycystic Ovary Syndrome (PCOS) is a common endocrine disorder. The cause of PCOS is still unknown and several factors may be involved. Objective: This study aims to evaluate the effect of synbiotics on serum parameters such as Follicle-Stimulating Hormone (FSH), Luteinizing Hormone (LH), triglyceride, cholesterol, glucose, and insulin in rats with PCOS. Methods: In this study, 40 adult female Wistar rats weighing approximately 180-200 g were randomly divided into five groups of control, PCOS, PCOS+LPS, PCOS+Synbiotic, PCOS+LPS+Synbiotic. To induce PCOS, rats were treated with Lipopolysaccharide (LPS) for 21 days. At the end, serum levels of LH, FSH, and insulin were measured by the ELISA method, and blood glucose, cholesterol, and triglyceride levels were measured by the enzymatic method. The results were analyzed in SPSS v. 22 software using one-way ANOVA and Tukey’s Post Hoc test. Results: The levels of triglycerides, cholesterol, insuli...
International Journal of Radiation Biology
PURPOSE Despite the extensive efforts to treat the leading cause of neurodegenerative diseases (N... more PURPOSE Despite the extensive efforts to treat the leading cause of neurodegenerative diseases (ND), a little progress has been reported. Red light might affect ND through many specific mechanisms. The purpose of this investigation is to explore the effect of red light on the expression of low-density lipoprotein receptor-1 (LRP-1) and transient receptor potential ankyrin-1 (TRPA-1) gene in the hippocampus, and the serum melatonin level (SML) of the LPS-induced neuro-inflammated rats. MATERIALS AND METHODS Red-light therapy was implemented using a wavelength 630 nm under different light conditions and the passive avoidance (PA) and Y-Maze tests were employed to assess memory performance. To evaluate the LRP-1 and TRPA-1 genes expression, q-RT-PCR was performed. To measure the SML, ELISA was performed before and after the red-light radiation. RESULTS Lipopolysaccharide (LPS) caused memory impairment in both behavioral tests. Red-light therapy improved PA memory in all light conditions (p < 0.001). However, in Y-maze, only the red-light radiation during light and dark cycles, improved memory (p < 0.01and p < 0.001, respectively). In addition, red-light radiation caused significant increase in SML (P < 0.05). The LRP-1 and TRPA-1 genes expression increased significantly during the dark phase in the red light radiated group compared to non-radiated group (P < 0.001). CONCLUSIONS Taken together, the results suggest that red-light therapy can reduce the complications of memory impairment in rats. This study has found that red-light therapy demonstrates higher effect during the period of dark phase compared to light phase. No doubt, further experimental studies would help us to establish a greater degree of accuracy on this matter.
Physiology and Pharmacology
Lipopolysaccharide (LPS) has been widely used in animal modeling of inflammation-induced hyperalg... more Lipopolysaccharide (LPS) has been widely used in animal modeling of inflammation-induced hyperalgesia. Proinflammatory cytokines (TNF-α, IL-1β) are involved in the pathogenesis of pain. Cichorium intybus (CI) contains many compounds which inhibit inflammatory mediators. The present study was designed to evaluate the effect of CI root hydro-alcoholic extract (CE) and Esculetin (ESC) - a bioactive coumarin compound of CI- on LPS-induced pain and inflammation in male mice. CE constituents were evaluated by GC-MS. Two different concentration of CE (300 and 500 mg/kg/p.o.) and ESC (40 mg/kg/i.p.,o.) were used for experiments. Drugs were administered once daily for 7 days. On day 7, LPS (0.83 mg/kg/i.p.) was injected 30 min after the last drug administration. Blood samples were collected 90 min after LPS injection. Hot plate and formalin tests were performed 24 hours after LPS injection. Serum TNF-α and IL-1β levels were measured using ELISA. LPS significantly increased the response time ...
Iranian Journal of Pharmaceutical Research : IJPR, 2018
miRNAs as one of the potential therapeutic agents have been recently considered for cancer treatm... more miRNAs as one of the potential therapeutic agents have been recently considered for cancer treatment. AS1411 (aptNCL) is a DNA aptamer specifically binding to nucleolin protein on the cancer cell surface with antiproliferative effect. The aim of the study was to develop a conjugate consisting of aptNCL (as targeted delivery of therapeutic agent) and miRNA let-7d (as a tumor suppressor) using two different linking methods and also to evaluate the potential effect of the conjugates on the proliferation of gastric cancer (MKN-45) cell line compared to negative control cell line of human dermal fibroblast (HDF). Conjugation was performed covalently by SM (PEG)2 as a bifunctional crosslinker (conjugate-1) and noncovalently, using 19bp complementary sticky end sequences (conjugate-2). Nucleolin positive MKN-45 and nucleolin negative HDF cells were cultured and treated with the conjugates. Then, the changes in let-7d expression and cell proliferation were determined using Real-time PCR and...
Introduction: An important issue in cancer therapy is the achievement of desired therapeutic resp... more Introduction: An important issue in cancer therapy is the achievement of desired therapeutic response with the least adverse effects. To achieve this goal, targeted drug delivery systems were developed. Aptamers, mainly DNA/RNA aptamers, are the attractive affinity ligands for the cancer cell surface specific antigens. Besides, microRNAs are another type of therapeutic and diagnostic oligonucleotides that have been recently studied in various cancers. miRNAs are small double stranded RNAs with important roles in cell regulatory pathways. Profile changes of miRNAs can result in cancer development. External addition of miRNAs or their elimination using antagomiR can lead to the efficient treatment of related disease. Targeted delivery of therapeutic agents to the site of action with less adverse effects is the most challenging issue in anticancer chemotherapeutic agents as well as miRNA therapy. In addition, miRNAs stability in biological systems can be improved by targeting strategy....
Basic and Clinical Neuroscience Journal, 2021
Introduction: Viola plant has been used traditionally to treat neurological disorders. We aimed a... more Introduction: Viola plant has been used traditionally to treat neurological disorders. We aimed at determining whether pretreatment with Viola spathulata extract can alleviate the severity of ischemic-reperfusion damages and exert its protective effects through the regulation of a sodium/calcium exchanger (NCX3) gene expression in a rat brain. Methods: Male Wistar rats were divided into two main groups: one main group for evaluating Neurologic Deficit Score (NDS) and Infarct Volume (IV) and the other group for the evaluation of NCX3 gene expression in the brain tissue. The latter group was subdivided into the intact, control (vehicle), sham, V5, and V10. The vehicle (control) subgroup received Dimethyl Sulfoxide (DMSO), and V5 and V10 subgroups received V. spathulata extract at the doses of 5 and 10 mg/kg (IP), respectively, for 7 days. After pretreatment, we carried out Middle Cerebral Artery Occlusion (MCAO) for 60 min. Results: In the V5 and V10 subgroups, NDS and IV significantl...
Caspian Journal of Neurological Sciences, 2021
Background: Pathological conditions, including ischemic stroke, are associated with severe Endopl... more Background: Pathological conditions, including ischemic stroke, are associated with severe Endoplasmic Reticulum (ER) stress that induces apoptosis and cell death. Herbal medicines are natural treatments with few side effects in such situations. Objectives: In the present study, we examined the probable neuroprotective effects of Viola spathulata, Lamium album, and Urtica dioica on splicing of ER stress mRNA gene marker (X-box Binding Protein-1 [XBP-1]) and Unfolded Protein Response (UPR) activation in the brain of the rat stroke model. Materials & Methods: Sixty male Wistar albino rats (weight: 220-250 g) were selected for this study and divided into two major groups. The first major group comprised the healthy animals that are subdivided into groups number 1 to 5. The second major group comprised the Middle Cerebral Artery Occlusion (MCAO) group subdivided into groups number 6-10. The reverse transcriptionpolymerase chain reaction was performed after pretreatment with different he...
Klimik Dergisi/Klimik Journal, 2021
Amaç: Sistemik bakteriyel endotoksin maruziyeti hepatik dokularda LUCOGS ("lipopolysaccharide (LP... more Amaç: Sistemik bakteriyel endotoksin maruziyeti hepatik dokularda LUCOGS ("lipopolysaccharide (LPS)-unfolded protein response (UPR)-calcium overload-gene splicing") açısından risk oluşturmaktadır. LUCOGS mekanizması ve sepsisle ilişkili süreci başlatmak üzere birbirleriyle etkileşimi tam olarak açığa kavuşmamıştır. Bu in vivo çalışma, hepatik uçbirleştirme ve UPR arasındaki ilişkinin aydınlatılmasını amaçlamaktadır. Yöntemler: Aydınlatılmış onam sonrasında hastaların yanık yaralarından Pseudomonas aeruginosa izolasyonu gerçekleştirilmiş ve LPS elde edilmiştir (hLPS). Erkek c57/BL6 fareler 6 gruba ayrılmıştır: 1. negatif kontrol (steril, apirojen normal salin) 2. pozitif kontrol (Tunikamisin) , 3. hLPS (3 mg/kg/intraperitoneal), 4. hLPS+ Dantrolen sodyum (kalsiyum kanal blokeri, CCI1) (A), 5. hLPS+2-aminometil fenil borinat (1 mg/kg) (CCI2) (B) ve 6. hLPS+CCI1/CCI2 (C). Uygulamalardan 2, 8 ve 24 saat sonra farelerin karaciğerleri adaptif UPR aktivasyonunun göstergesi olan XBP-1 birleştirmesinin değerlendirilmesi için çıkarılmıştır. Bulgular: hLPS grubuna kıyasla kombine hLPS ve CCl gruplarında XBP-1 birleştirme sayısı anlamlı olarak artış göstermiştir. Kontrollere kıyasla en yüksek XBP-1 birleştirme sayısı, Grup C' de 8 ve 24 saat sonra görülmüştür. Sonuçlar: Elde ettiğimiz veriler, CCl ile kombinasyonun, UPR gen uçbirleştirmesi modifikasyonu aracılığıyla hLPS ile indüklenmiş proinflamatuar aktivasyonu engelleyebileceğini göstermektedir. İki tür CCl uygulaması yaklaşımı ile indüklenmiş endotoksemi sonrasında hücre içi kalsiyum aşırı yüklenmesinin engellenmesi mümkün olabilir. Ayrı ayrı uygulanmalarına kıyasla kalsiyum kanal blokerlerinin birlikte uygulanmaları, (pro) inflamatuar süreçleri inhibe etme etkinliğini artırdığından sistemik dolaşımda LUGOGS yükselmesinin durdurulması ve organ yetmezliğinin engellenmesi için yeni bir strateji olarak değerlendirilebilir. Öne sürülen bu etkileşim mekanizmasının in vivo olarak daha detaylı ortaya konması için yeni uygulamanın etkinliğinin başka çalışmalarla desteklenmesi gereklidir.
Advances in Experimental Medicine and Biology, 2021
In the COVID-19 era, while we are encouraged to be physically far away from each other, social an... more In the COVID-19 era, while we are encouraged to be physically far away from each other, social and scientific networking is needed more than ever. The dire consequences of social distancing can be diminished by social networking. Social media, a quintessential component of social networking, facilitates the dissemination of reliable information and fighting against misinformation by health authorities. Distance learning, telemedicine, and telehealth are among the most prominent applications of networking during this pandemic. Additionally, the COVID-19 pandemic highlights the importance of collaborative scientific efforts. In this chapter, we summarize the advantages of harnessing both social and scientific networking in minimizing the harms of this pandemic. We also discuss the extra collaborative measures we can take in our fight against COVID-19, particularly in the scientific field.
Disaster Medicine and Public Health Preparedness, 2020
Journal of Current Biomedical Reports, 2021
The novel coronavirus, termed severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) is a m... more The novel coronavirus, termed severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) is a major public health challenge all over the world and is the causative agent of coronavirus disease 2019 (COVID-19). Since December2019 the outbreak of COVID-19 has become a major epidemic issue all over world. In this pandemic, preterm and term neonates with infected mothers are becoming more vulnerable each day. Although we mostly witness asymptomatic neonates, getting symptomatic may not be a rarity in the future. After entrance of COVID-19 into the body it could affect the balance of microbiota combination which can result in microbiota dysbiosis and eventually leads to immune imbalance. Intrauterine microbiome dysbiosis in COVID-19 positive mothers and transmission of lipopolysaccharide (LPS) may restructure the environment of the developing fetus with possible short/long-term impact on the individual's health and disease. Therefore, assessing the changes in microbiome of neonates from infected mothers via exploring meconium could be valuable. It is also logical to measure LPS level and balanced its levels by using prebiotics and probiotics as a supplemental therapeutic procedure to prevent medical challenges in future. The aim of this essay is to review the potential conception that detection of COVID-19 and the meconium microbiota and LPS quantity could be used as a source of prognostic information about the COVID-19 severity in the fetus of pregnant women with COVID-19.
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Papers by mojtaba hedayati