Papers by mohammad reza Rahmany
Immunology Letters, Feb 1, 2018
In vivo and in vitro aging of the mesenchymal stromal cells (MSCs) can affects their properties. ... more In vivo and in vitro aging of the mesenchymal stromal cells (MSCs) can affects their properties. We investigated the immunomodulatory properties of the term and preterm human umbilical cord vein MSCs (UCV-MSCs) at the passages (P) 2 and 5. Term and preterm UCV-MSCs at P2 and 5 were co-cultured with two-way mixed lymphocyte reaction. Proliferation, IFN-γ and IL-10 protein levels, mRNA levels of the COX-2, TGF-β1, TNF-α, IL-4 and FoxP3 were assessed. The term UCV-MSCs and P5 of the term and preterm UCV-MSCs had stronger inhibitory effects on cell proliferation than the preterm UCV-MSC and P2, respectively (P = 0.001). In supernatants of the co-cultures, IFN-γ was higher in the term UCV-MSC than the preterm UCV-MSC, while IL-10 was higher in the preterm UCV-MSCs than the term UCV-MSCs. Also in the co-cultures, COX-2 expression in the term UCV-MSCs and P2 was higher than the preterm UCV-MSCs and P5, respectively and TGF-β1 expression in the term UCV-MSCs was higher than preterm. Conclusively it appears that the term UCV-MSCs, and P5 of the term and preterm UCV-MSCs showed a higher immunomodulatory ability than the preterm UCV-MSCs and P2, respectively.
Food and Agricultural Immunology, 2016
Licorice has immunomodulatory and anti-asthmatic properties. This study aims to test the effect o... more Licorice has immunomodulatory and anti-asthmatic properties. This study aims to test the effect of licorice on pulmonary fibrosis (PF). PF was induced in mice through bleomycin instillation. One group of the mice received bleomycin (positive control), another one received phosphate-buffered saline and the other two received 300 mg/kg of the extracts for 20 days after the bleomycin instillation. Hematoxylin-eosin and Masson's staining were used in the lung tissues. The expression of α-SMA, TGF-β1, IL-1β, and TNFα were evaluated using reverse-transcriptase-polymerase-chainreaction. Bleomycin led to tissue-degenerative changes, collagen deposition, and overexpression of the genes in the lung tissues. In the mice receiving the aqueous extract, the weight gain was higher (p < .004), and tissue-degenerative changes (p < .001) and collagen deposition were lower than the positive control. The expressions of the genes were lower than the positive control. These results were not observed in the hydroalcoholic extract. Aqueous extract of licorice alleviated the severity of PF.
The initiating event in multiple sclerosis (MS) pathogenesis is not known yet. However, in genera... more The initiating event in multiple sclerosis (MS) pathogenesis is not known yet. However, in general, breakdown of the blood-brain barrier (BBB) and subsequent infiltration of immune cells into the central nervous system (CNS) has been thought to be the main initiating event. Nonetheless, the mechanism by which the BBB gets disrupted and allows immune cells to infiltrate into the CNS is not fully understood. Evidence indicates that prior to cellular infiltration, over passing peripherally generated cross-reactive immunoglobulin G (IgG) through the transiently permeable BBB during systemic inflammation, hypoxia, hyperthermia, transient hypertension or acute stresses may cause CNS inflammation, BBB breakdown and then initiation of MS disease. Here, we discuss the possible detailed mechanisms that may be involved in cross-reactive IgG-mediated MS autoimmunity.
Journal of The Australian Traditional-medicine Society, 2014
Introduction: Helicobacter pylori is a common human pathogen associated with many gastrointestina... more Introduction: Helicobacter pylori is a common human pathogen associated with many gastrointestinal (GI) problems, especially gastric ulcers and cancer. Gastric cancer is one of the major causes of death throughout the world. Gum of Commiphora myrrha, as a medicinal plant, is reported to have antibacterial and anticancer effects. Materials and methods: The ethanol extract of gum of Commiphora myrrha was prepared using 85% ethanol hydroalcoholic solvent. The anti-H. pylori effect was evaluated using the disc diffusion and agar dilution methods against three clinical isolates of H. pylori. Effects of the extract on the human gastric carcinoma AGS cells were assessed using staining with the trypan blue method and MTT assay. Furthermore, effects of the extract on peripheral blood mononuclear cells (MNCs) and mesenchymal stem cells (MSCs) as normal cells were evaluated. Results: The mean inhibition zone diameter of the extract against H. pylori was 30 mm, and the minimum inhibitory concen...
Iranian journal of parasitology, 2013
The effect of frequent examinations on the students' learning has had inconsistent results. T... more The effect of frequent examinations on the students' learning has had inconsistent results. This study aimed to assess the effectiveness of frequent announced quizzes on the learning of a representative sample of Iranian medical students. This experimental study was conducted among 37 fifth semester medical students who had taken the course in Protozoology and Helminthology, in which the same basic information were provided about different types of protozoa and worms. Initially, in the teaching of helminthology, ten routine sessions were handled with lectures and interactive questions and answers. Then at the beginning of the protozoology topic in the beginning of all of the next 9 sessions, the students were informed that they will have a quiz at the end of each session. At the end of the semester, the total scores of quizzes were compared with the mean final scores of protozoology and helminthology using paired t and repeated measure tests. The mean final scores of the protozo...
Immunology letters, 2018
In vivo and in vitro aging of the mesenchymal stromal cells (MSCs) can affects their properties. ... more In vivo and in vitro aging of the mesenchymal stromal cells (MSCs) can affects their properties. We investigated the immunomodulatory properties of the term and preterm human umbilical cord vein MSCs (UCV-MSCs) at the passages (P) 2 and 5. Term and preterm UCV-MSCs at P2 and 5 were co-cultured with two-way mixed lymphocyte reaction. Proliferation, IFN-γ and IL-10 protein levels, mRNA levels of the COX-2, TGF-β1, TNF-α, IL-4 and FoxP3 were assessed. The term UCV-MSCs and P5 of the term and preterm UCV-MSCs had stronger inhibitory effects on cell proliferation than the preterm UCV-MSC and P2, respectively (P = 0.001). In supernatants of the co-cultures, IFN-γ was higher in the term UCV-MSC than the preterm UCV-MSC, while IL-10 was higher in the preterm UCV-MSCs than the term UCV-MSCs. Also in the co-cultures, COX-2 expression in the term UCV-MSCs and P2 was higher than the preterm UCV-MSCs and P5, respectively and TGF-β1 expression in the term UCV-MSCs was higher than preterm. Conclus...
Leukocyte infiltration and acinar cell injury are characteristic features of acute pancreatitis (... more Leukocyte infiltration and acinar cell injury are characteristic features of acute pancreatitis (AP). However, the signaling pathways regulating inflammation and accumulation of leukocytes into pancreas tissue remains poorly elucidated. In the current study, we investigated the effects of Glycyrrhizin (GZ) on cerulein-induced AP in mice. AP was induced in male C57BL/6 by intraperitoneal injection of 50 μg/kg cerulein hourly, with a total of 7 times. 1 hour after the last injection of cerulean, mice were treated with either 35 or 70 mg/kg of GZ. Serum amylases and lipases were measured using automated chromogenic assay, MCP-1 and MIP-2 concentrations were measured in the se- rum by ELISA, and the number of infiltrated inflammatory cells in the pancreas were evaluated by flow cytometry. We found that GZ treatment resulted in reduction (i) both amylase and lipase activities, (ii) the serum levels of both MCP-1 and MIP-2; and (iii) markedly attenuated cerulein-induced histopathological ...
Iranian Journal of Allergy, Asthma and Immunology, 2020
Co-inhibitory molecules modulate immune responses. Immunomodulatory properties of mesenchymal ste... more Co-inhibitory molecules modulate immune responses. Immunomodulatory properties of mesenchymal stem cells (MSCs) turn them into ideal candidates for cell therapy. This study was designed to investigate the immunomodulatory effect of adipose-derived stem cells (ASCs) on inflammatory environment of a co-culture of allogenic peripheral blood mononuclear cells (PBMCs) in a two-way mixed leukocyte reaction (twMLR) setting. ASCs were co-cultured with allogenic PBMCs in twMLR setting for four days. The proliferation of peripheral blood mononuclear cells (PBMCs), levels of interleukin (IL)-10, and expression of interferon-gamma (IFN-γ), B7-1, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), programmed death-ligand 1 (PD-L1), +, and CD200R1 genes, as well as cell surface expression of CD200 and CD200R1, were measured in twMLR as control, and co-culture groups on days 0, 2 and 4 of the experiment. The proliferation of PBMCs was suppressed on days 2 and 4 of co-culture. The expression of ...
Journal of Interferon & Cytokine Research, 2022
Cortisol is secreted in prolonged stress and has therapeutic effects in inflammatory diseases. Co... more Cortisol is secreted in prolonged stress and has therapeutic effects in inflammatory diseases. Considering the immunomodulatory effects of mesenchymal stem cells, here we investigated the effect of hydrocortisone (HC) long-term treatment on immunomodulatory properties of human adipose-derived mesenchymal stromal/stem cells (ASCs). Isolated ASCs from healthy subjects were treated with different HC concentrations for 14 days. The effect of HC-treated ASCs on the proliferative response of peripheral blood mononuclear cells (PBMCs) was evaluated in ASCs/2-way mixed leukocyte reaction coculture using 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT)-assay. HC-treated ASCs were further divided into interferon gamma (IFN-γ) stimulated and unstimulated groups. Transforming growth factor beta 1 (TGF-β1) and interleukin (IL)-6 levels were measured in culture supernatants by enzyme-linked immunosorbent assay. Relative expression of cyclooxygenase-2 (COX-2), hepatocyte growth factor, indoleamine dioxygenase, and programmed death-ligand 1 genes was assessed by real-time PCR. Levels of TGF-β1 and COX-2 expression were elevated in unstimulated ASCs, while exposure to high concentration of HC significantly increased TGF-β1 levels and reduced COX-2 expression. Unstimulated HC-5-μM-treated ASCs increased PBMC proliferation ratio on day 2 of coculture compared to the control group (P = 0.05). In IFN-γ stimulated condition, pretreatment with HC-5 μM resulted in a significantly increased IL-6 and significantly decreased COX-2 expression compared to the HC untreated control group. In conclusion, our results showed various alterations of ASC immunomodulatory related features as a result of long-term exposure of different concentrations of HC. It seems that HC at low concentration pushed the balance toward extended immune response in ASCs, while this observation wasn't persistent in ASCs treated with higher concentrations of HC.
Aims: During brucellosis, infection macrophages and T lymphocytes perform effectively to eliminat... more Aims: During brucellosis, infection macrophages and T lymphocytes perform effectively to eliminate microbes. Cytokines are small proteins that have role in activation, differentiation and performance of immune cells. The cytokine secretory pattern can be important in clinical brucellosis outcome. The purpose of this study was to determine the levels of some important cytokines in acute and chronic brucellosis patients during immune response. Methods: In this case-control study which was performed in Sanandaj's Tohid Hospital, Iran, 63 patients referred to infectious department were studied. 30 healthy persons were selected as control. 12ml of blood was taken from each patient for performing brucellosis diagnosing tests like serological tests, blood cultures and PCR. Cytokines were measured by using Sandwich-ELISA. Data were analyzed by SPSS 12 software using independent T-test and Mann-Whitney U tests. Results: The mean serum levels of IL-6, IL-8, IL-10, IFN-γ and TGF-β in acute...
Advanced Pharmaceutical Bulletin
Purpose: Acute pancreatitis (AP) which is distinguished by local pancreatic necrosis, following b... more Purpose: Acute pancreatitis (AP) which is distinguished by local pancreatic necrosis, following by systemic organ failure is known as an inflammatory disease. Up to now, there are only a few treatment options accessible for patients suffering from AP. In this study, we aimed to examine the anti-inflammatory capacities of human bone marrow-derived mesenchymal stromal cells (hBM-MSC) in a detailed AP model experiment. Methods: AP was induced in C57BL/6 mice by intraperitoneal administration of cerulein (100 µg/kg/h × 7 doses) at intervals of 1 hour (h). Then, 2×105 MSCs were infused in the AP mice by tail vein 6 h after the last cerulein injection. Mice were sacrificed 12 h following the injection of hBM-MSC, and blood samples and pancreas tissues were obtained. Results: We first determined the presence of transplanted hBM-MSC in the pancreas of mice with AP, but not the control mice. Our data indicate that administration of hBM-MSCs to mice with AP lead to (i) decreased serum levels ...
Physiology and Pathology of Immunology, 2017
Journal of the Egyptian National Cancer Institute, 2022
Background Despite antitumor properties, chemotherapy medication can create conditions in tumor c... more Background Despite antitumor properties, chemotherapy medication can create conditions in tumor cells that work in favor of the tumor. Doxorubicin, commonly prescribed chemotherapy agents, can increase the risk of migration and invasion of tumor cells through overexpression of the CXCR4 gene by affecting downstream signaling pathways. The regulatory role of CXCR7 on CXCR4 function has been demonstrated. Therefore, it is hypothesized that combining doxorubicin with another anticancer drug could be a promising approach. Methods In this research, we evaluated the anti-invasive property of pioglitazone along with antitumor effects of doxorubicin on MDA-MB-231 breast cancer cell lines. Results There was no significant difference between two treatment groups in neither the expression nor changes in the expression of CXCR7 and CXCR4 genes (P < 0.05). Pioglitazone-doxorubicin combination reduced cell migration in tumor cells to a significantly higher extent compared to doxorubicin alone ...
Immunological Investigations, 2020
We describe a cohort of 25 Iranian patients with infantile inflammatory bowel disease (IBD), 14 (... more We describe a cohort of 25 Iranian patients with infantile inflammatory bowel disease (IBD), 14 (56%) of whom had monogenic defects. After proper screening, patients were referred for whole exome sequencing (WES). Four patients had missense mutations in the IL10 RA, and one had a large deletion in the IL10 RB. Four patients had mutations in genes implicated in host:microbiome homeostasis, including TTC7A deficiency, and two patients with novel mutations in the TTC37 and NOX1. We found a novel homozygous mutation in the SRP54 in a deceased patient and the heterozygous variant in his sibling with a milder phenotype. Three patients had combined immunodeficiency: one with ZAP-70 deficiency (T + B + NK −), and two with atypical SCID due to mutations in RAG1 and LIG4. One patient had a G6PC3 mutation without neutropenia. Eleven of the 14 patients with monogenic defects were results of consanguinity and only 4 of them were alive to this date.
The immunomodulatory properties of type 2 diabetic patients' adipose-derived mesenchymal stem... more The immunomodulatory properties of type 2 diabetic patients' adipose-derived mesenchymal stem cells (D-ASCs) has not been extensively studied. In this study, we compared the immunomodulatory properties of D-ASCs and non-diabetic subjects mesenchymal stem cells (ND-ASCs) in co-culture with mixed leukocyte reaction (MLR). ASCs were isolated from adipose tissue samples of type 2 diabetic and non-diabetic subjects (age: 40-55). D-ASCs and ND-ASCs were co-cultured with two-way MLR. Peripheral blood mononuclear cells (PBMCs) proliferation ratio, protein levels of IFN-γ and IL-10, mRNA expression of COX-2, TNF-α, TGF-β1 and IL-6 genes in MLR, D-ASCs and ND-ASCs co-cultures were assessed using XTT, ELISA and Real-time qRT-PCR, respectively. PBMCs proliferation on days 2 and 4 of D-ASCs co-culture was higher than ND-ASCs co-culture of the same days (p < 0.001). IFN-γ level decreased on day 4 compared to day 2 of ND-ASCs co-culture, but its level had not changed in D-ASCs co-culture. C...
Advanced Pharmaceutical Bulletin, 2019
Purpose: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disorder with few available tr... more Purpose: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disorder with few available treatments. Mesenchymal stem cell therapy (MSCT), an innovative approach, has high therapeutic potential when used to treat IPF. According to recent data, preconditioning of MSCs can improve their therapeutic effects. Our research focuses on investigating the anti-inflammatory and antifibrotic effects of H2 O2 -preconditioned MSCs (p-MSCs) on mice with bleomycin-induced pulmonary fibrosis (PF). Methods: Eight-week-old male C57BL/6 mice were induced with PF by intratracheal (IT) instillation of bleomycin (4 U/kg). Human umbilical cord vein-derived MSCs (hUCV-MSCs) were isolated and exposed to a sub-lethal concentration (15 μM for 24 h) of H2 O2 in vitro. One week following the injection of bleomycin, 2×105 MSCs or p-MSCs were injected (IT) into the experimental PF. The survival rate and weight of mice were recorded, and 14 days after MSCs injection, all mice were sacrificed. Lung tissue was...
International Journal of ADVANCED AND APPLIED SCIENCES, 2018
Aspergillus is a filamentous fungus of wide distribution having widespread species diversity. Amo... more Aspergillus is a filamentous fungus of wide distribution having widespread species diversity. Among pathogenic species of Aspergillus, Aspergillus fumigatus, Aspergillus niger, and Aspergillus flavus are regarded as the most important pathogenic agents. The aim of this research is to study the antifungal effects of Artemisia dracunculus L, Achillea wilhelmsii C. Koch, Bunium persicum, Cuminum cyminum L, Zataria multiflora Boiss, and Satureja hortensis extracts against Aspergillus Sp. The herbal extracts were prepared using maceration method. 50gr of ground plant was introduced into a 1-litre flask and macerated with 400ml of 70% ethanol for 24h and then was shaken on shaker. Afterwards, different concentrations of the extract were prepared in dimethyl sulfoxide solvent (DMSO). The extracts were filtered and sterilized. Suspension of each fungus was separately prepared with 1×10 6 CFU/ml concentration. The antifungal effects of the extracts were measured through NCCLS protocols and Broth dilution method. The control and comparison were done between the antifungal effects of the mentioned herbal extracts and Amphotericin B and Nystatin. The results indicated that the range of MIC and MFC values of these drugs was significantly higher (p≤0.05) compared to the prepared herbal extracts of different dilutions and control group of drug solvent, too. Results showed that the extracts of all herbal extracts have antifungal properties against Aspergillus sp.
Iranian Biomedical Journal, 2019
Background: Mesenchymal stem cells (MSCs) can be used to treat premature ovarian failure (POF). D... more Background: Mesenchymal stem cells (MSCs) can be used to treat premature ovarian failure (POF). Different methods have already been applied to detect MSCs in tissues. This study aimed to investigate the quantitative distribution of CM-DiI-labeled human umbilical cord vein MSCs (hUCV-MSCs) in different regions of the ovarian tissue of the cyclophosphamide (CTX)-induced POF in mice. Methods: Adult female C57BL/6 mice (n = 40) were divided into four groups: (1) Mice receiving PBS as control (Ctrl) group; (2) mice receiving hUCV-MSCs intravenously as Ctrl + hUCV-MSCs group; (3) mice receiving CTX intraperitoneally (i.p.) as CTX group; (4) mice receiving CM-DiI-labeled hUCV-MSCs after CTX injection as CTX + hUCV-MSCs group. Histological changes and CM-DiI-labeled hUCV-MSCs distribution were analyzed in the ovarian tissues. Quantitative real-time PCR was performed to detect human mitochondrial cytochrome b (MTCYB) gene in the ovarian tissues of the mice. Results: The mean number of the fluorescent hUCV-MSCs was 20 ± 2.5 (57.1%) in the medulla, 11.3 ± 2.8 (32.2%) in the cortex, and 5.5 ± 1 (15%) in the germinal epithelium of the ovarian tissue (p < 0.05). Moreover, MTCYB gene was detected in the mice ovaries of the CTX + hUCV-MSCs group, but not in other groups. Conclusion: Our findings suggest that the distribution of the transplanted hUCV-MSCs in different regions of the ovarian tissue is not equal, and it is greater in the medulla than the cortex and germinal epithelium. This is the first report of quantitative distribution of MSCs in different regions of ovarian tissue in the POF model.
Advances in medical sciences, Jan 6, 2017
Migration ability of mesenchymal stem cells (MSCs) towards chemotactic mediators is a determinant... more Migration ability of mesenchymal stem cells (MSCs) towards chemotactic mediators is a determinant factor in cell therapy. MSCs derived from different sources show different properties. Here we compared the migration ability of the term and the pre-term human umbilical cord vein MSCs (hUCV-MSCs). MSCs were isolated from term and pre-term umbilical cord vein, and cultured to passage 3-4. Migration rate of both groups was assessed in the presence of 10% FBS using chemotaxis assay. Surface expression of CXCR4 was measured by flow cytometery. The relative gene expression of CXCR4, IGF1-R, PDGFRα, MMP-2, MMP-9, MT1-MMP and TIMP-2 were evaluated using real time PCR. The isolation rate of the pre-term hUCV-MSCs was higher than the term hUCV-MSCs. Phenotype characteristics and differentiation ability of the term and pre-term hUCV-MSCs were not different. The migration rate of the pre-term hUCV-MSCs was more than the term hUCV-MSCs. Gene and surface expressions of the CXCR4 were both signific...
Iranian journal of allergy, asthma, and immunology, 2017
In recent studies, mesenchymal stromal cells (MSCs) have been increasingly employed to treat vari... more In recent studies, mesenchymal stromal cells (MSCs) have been increasingly employed to treat various diseases like pulmonary fibrosis (PF). There are very few MSCs in tissues so in order to obtain their sufficient numbers for therapeutic applications, their in vitro expansion is necessary. The aim of this study was to investigate the effects of long-term culture of the human umbilical cord vein MSCs (hUCV-MSCs) on pulmonary fibrosis in mice. MSCs were first isolated from human umbilical cord vein and cultured up to 18 passages. In C57BL/6 mice, 15 min after belomycin instillation, UCV-MSCs at passages (P) 0, 4, 8, 12, and 18 (long-term culture) were transplanted intratracheally. Mice were weighted every 5 days and were euthanized on day 21. For histopathological examination, the lung sections were stained with hematoxylin-eosin (HE) and Masson's trichrome. The mRNA expression of TGF-β1, alpha-smooth muscle actin (α-SMA), and collagen type I alpha 1 (COL1A1) in lung tissues were ...
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Papers by mohammad reza Rahmany