kurtulus buruk

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Ekstrakardiak aterosklerotik arter hastalıklarında Chlamydia pneumoniae'nin rolü

Türk Göğüs Kalp Damar Cerrahisi Dergisi, 2001

Sağlık Çalışanlarında Nazal Metisiline Dirençli Staphylococcus Aureus (MRSA) Taşıyıcılığının Araştırılması

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi, 2021

Çalışmada ilgili kurumdan ve Üniversitenin Etik Kurulu'ndan izin alınmıştır. Bu çalışma birinci y... more

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Investigation of Antimicrobial Resistance Profiles of Gram Negative Bacteria Isolated from Ornamental Fish Aquariums in Petshops

Turkish Bulletin of Hygiene and Experimental Biology, 2017

Objective: In aquariums in shops which sell ornamental fish, a large number of fish are present t... more Objective: In aquariums in shops which sell ornamental fish, a large number of fish are present together, and many microorganisms can grow in them. Moreover, since the fish which are sold are transferred with some water the microorganisms are transported to different aquariums as well. A variety of antimicrobials can be added to aquariums to prevent or treat fish diseases during the breeding of ornamental fish, and this can lead to the development of antimicrobial resistance. The aim of this study is to determine the presence and antimicrobial susceptibility of Gram negative bacteria isolated from water samples taken from ornamental fish aquariums in stores where pets are sold. Methods: Water samples were collected from 27 ornamental fish aquariums in five pet shops serving in Trabzon. These specimens were inoculated on 5% Sheep Blood Agar and Eosin-Methylene-Blue Agar media. Colonies from plaques were identified using MALDI-TOF MS. Antimicrobial susceptibility tests were performed using the Kirby-Bauer disc diffusion method. Results: A total of 31 Gram negative bacteria were identified in the 27 samples of ornamental fish aquarium water taken from the five pet shops. Citrobacter freundii was detected most frequently with a rate of 25.8%.

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New Coronavirus: SARS-COV-2

Mucosa, 2020

Dünya Sağlık Örgütü, 2020 yılının ilk günlerinde, dünyanın potansiyel pandemi ajanı olan yeni bir... more Dünya Sağlık Örgütü, 2020 yılının ilk günlerinde, dünyanın potansiyel pandemi ajanı olan yeni bir koronavirüs ile karşı karşıya olduğunu bildirmiştir. Etiyolojik ajanı Coronaviridae ailesinden, 2002 yılı Kasım ayında yine Çin'de ortaya çıkan SARS-CoV ile aynı alt aileye konumlandırılan SARS-CoV-2'dir. Etken zarflı, pozitif polariteli bir RNA virüsüdür. Yarasa kökenli olduğu düşünülmektedir. Yaptığı hastalık başlangıçta ölümcül olmayan bir pnömoni olarak tanımlanırken günümüzde %3.4 ölüm oranına sahip Covid-19 hastalığı olarak nitelendirilmektedir. Virüs, dolayısıyla hastalık, 2019 yılının sonlarında Çin'de başlamış, 2020'nin Mart ayının ortalarında Türkiye dâhil 133 ülke ve özerk bölgeye yayılmıştır. Yüksek ateş, takip eden kuru öksürük, yorgunluk, balgam üretimi, solunum güçlüğü, boğaz, baş, kas ve eklem ağrıları en sık görülen hastalık belirtileridir. Yaşlı ve bazı komorbid hastalığı olanlarda ölümcül seyredebilmektedir. Tedavisinde çeşitli antiviraller denenmesine karşın henüz onaylı tedavi bulunmamaktadır, daha çok semptomatik etkili ajanlar kullanılmaktadır. Aşı geliştirme çalışmaları sürdürülmektedir. Bu nedenle günümüzde en etkili korunma yolu, doğru kişisel hijyen davranışlarını önemseyerek uygulama ve bazı sosyal davranışların ertelenmesidir.

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Investigation of Human Papillomavirus (HPV) and Epstein-Barr Virus (EBV) in antrochoanal polyps

American Journal of Otolaryngology, 2019

Methods: A total of 100 patients (including 43 patients undergoing surgery for antrochoanal polyp... more Methods: A total of 100 patients (including 43 patients undergoing surgery for antrochoanal polyp, 27 patients undergoing surgery for nasal polyp, and 30 patients undergoing surgery for hypertrophic inferior turbinate) were included in this study. DNA was isolated from formalinfixed, paraffin-embedded samples with the aid of the Bioneer's AccuPrep Genomic DNA Extraction Kit. In the obtained genomic DNAs, while the detection of HPV DNA was performed using the nested-PCR method, the detection of HPV types 11/16 and EBV DNA was performed using the RT-PCR method. Results: The mean age of the patients with antrochoanal polyp was 26.7±15.4 years (range 7-70). There were 20 (46.5%) women and 23 (53.5%) men in the antrochoanal polyp group. HPV DNA was positively detected using the nested-PCR method in 14 (32.6%) of the patients with antrochoanal polyp and in 3 (11.1%) of the patients with nasal polyp. HPV DNA was not detected in the hypertrophic inferior turbinate group (control group). There was a statistically significant difference between all groups in terms of HPV DNA positivity. In the antrochoanal polyp group, 2 patients had HPV 11 positivity and 12 patients had HPV 16 positivity. In the nasal polyp group, 1 patient had HPV 11 positivity and 2 patients had HPV 16 positivity. EBV DNA was positively detected in 16 (37.2%) of the patients with antrochoanal polyp, in 11 (40.7%) of the patients with nasal polyp and in 8 (26.7%) of the patients with hypertrophic inferior turbinate, respectively. There was no statistically significant difference between the groups in terms of EBV DNA positivity. Conclusions: This study demonstrates that there is a need for further studies investigating the presence of viruses in antrochoanal polyps.

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Correlation between architect hepatitis C virus (HCV) core antigen and HCV Ribonucleic acid levels in Anti-HCV reactive patients in Turkey

African Journal of Microbiology Research, Oct 11, 2013

Quantitative tests for the detection of hepatitis C virus ribonucleic acid (HCV-RNA) levels and H... more Quantitative tests for the detection of hepatitis C virus ribonucleic acid (HCV-RNA) levels and HCV core antigen have been mainly used for the management of patients with HCV infection. The aim of this study was to evaluate the correlation between HCV core antigen and HCV RNA levels in patients reactive for anti-HCV antibodies. Three hundred and twenty-five anti-HCV reactive sera samples were included in the study. HCV core antigen and HCV RNA levels were determined using the Architect HCV Ag test and Abbott RealTime TM HCV RNA test (RT-PCR), respectively. The correlation coefficient between the levels of HCV core antigen and HCV RNA test results was calculated using Spearman's rank test, and linear regression analysis was applied. One hundred and sixteen of the 325 samples were detected positive by both methods. Three additional samples by RT-PCR, and 4 samples by Architect HCV Ag, the negative samples were found positive by the other method. All of these contradictory results were obtained from the low level HCV RNA or HCV core antigen including samples. A correlation coefficient (r) was determined as 0.899 between the levels of HCV core antigen and HCV RNA (p<0.0001). The sensitivity, specificity, positive predictive value and negative predictive value of the HCV core antigen test were 97.48, 98.06, 96.67 and 98.54%, respectively, using the HCV RNA test as a reference. The Architect HCV core antigen test exhibits a good correlation with the HCV RNA test. It can be used as an alternative method, especially when the HCV RNA test is unavailable.

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Chemical composition and antimicrobial activity of Anzer tea essential oil

Journal of the Science of Food and Agriculture, May 1, 2007

Anzer tea (Thymus praecox, subsp. caucasicus var. caucasicus) naturally grows in the eastern Blac... more Anzer tea (Thymus praecox, subsp. caucasicus var. caucasicus) naturally grows in the eastern Black Sea region of Turkey. Anzer tea, a creeping plant with crimson-pink flowers, is important for honey production in the region. In the present study, content, composition and antimicrobial properties of Anzer tea's essential oil were investigated. Essential oil content of dried aerial plant parts varied between 1.53% and 2.05%. Essential oil composition was studied by means of gas chromatography–mass spectrometry, and 26 components were identified. The major components were thymol (47.45%), γ-terpinene (8.73%), p-cymene (8.30%), terpinyl acetate (4.88%) and carvacrol (4.66%). Essential oil was also screened for its antibacterial activity. In a screen for antibacterial activity, Anzer tea essential oil had significant activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Candida albicans. Copyright © 2007 Society of Chemical Industry

Six-year distribution pattern of hepatitis C virus in Turkey: a multicentre study

Biotechnology & Biotechnological Equipment, 2015

ABSTRACT Hepatitis C infection is a public health problem. The aim of this retrospective study wa... more ABSTRACT Hepatitis C infection is a public health problem. The aim of this retrospective study was to determine the distribution of hepatitis C virus (HCV) genotypes in seven regions of Turkey, by evaluating 7002 patients with chronic HCV in a six-year period. During the 2009–2014 period, serum/plasma samples from 7002 new consecutive HCV RNA positive patients were collected. The female patients were 3867 (55.2%). The genotype distribution of HCV patiens was evaluated by ages and years. Statistical analysis was performed by using the Mann–Whitney test and the χ2 analysis. During the six-year period, genotype 1b was the most common genotype (67.7%) followed by untypeable genotype 1 (7.7%), genotype 4 (7.3%) and genotype 3 (6.7%). In 2014, genotype 3 was the second most common one (11.3%) and genotype 4 was the third most common one (9.8%). In the group with <25 years old patients, genotype 1b was most common (78.48%, 62/79) between the years of 2009 and 2011, whereas genotype 3 (34.8%, 86/247), between the years of 2012 and 2014. Genotype 1b was the most common in the groups between 26 and 35 years, 36 and 45 years, 46 and 55 years, 56 and 65 years. The rate of genotype 3 was increased from 4.78% to 10.06% and the rate of genotype 4 was increased from 1.3% to 3.84%, from 2009–2011 to 2012–2014. In recent years, genotypes 3 and 4 have gained importance. New therapeutic strategies and survey studies may be required for the modified HCV genotype pattern.

On Dört Aylık Bir Çocukta Primer Neisseria meningitidis Konjonktiviti

Mikrobiyoloji Bulteni, 2015

Meningokok konjonktiviti, ciddi komplikasyonlara yol açması ve halk sağlığını tehdit etmesi ile b... more Meningokok konjonktiviti, ciddi komplikasyonlara yol açması ve halk sağlığını tehdit etmesi ile büyük önem taşıyan nadir görülen bir enfeksiyondur. Primer ve sistemik bir enfeksiyondan sonra gelişen sekonder tip olmak üzere iki şekilde ortaya çıkabilmektedir. Primer meningokok konjonktivitinin, görme kaybı ile sonuçlanabilen oküler komplikasyonlara ilaveten sistemik meningokokal hastalık gibi ciddi komplikasyonlara da yol açabileceğinden, erken ve doğru tanısı çok önemlidir. Ancak, meningokok konjonktivitinin tanısında, diğer bakteriyel konjonktivitlerden ayıran özgül belirtilerin olmaması, kültür yapılmadan ampirik antibiyotik tedavisine başlanması ve laboratuvarlarda tanımlamada kullanılan ticari kitler/sistemler ile Neisseria gonorrhoeae ve Neisseria meningitidis'in ayrımının her zaman doğru olarak yapılamaması nedeniyle sorunlar yaşanmaktadır. Bu raporda, 14 aylık bir kız çocukta, serogruplandırılamayan N.meningitidis ile oluşan ve tedavi sonrasında sekelsiz iyileşen primer tek tarafl ı konjonktivit olgusunun sunulması amaçlanmıştır. Öncesinde sağlıklı olan 14 aylık kız çocuğu, iki gün önce başlayan sol gözünde kızarıklık, çapaklanma ve akıntı şikayetiyle pediatri acil birimine getirilmiştir. Yapılan göz muayenesinde sol konjonktivada hiperemi ve pürülan akıntı saptanmıştır. Pürülan konjonktivit tanısı konulan hastadan kültür için konjonktival sürüntü örneği alındıktan sonra, topikal netilmisin (4x1), topikal fusidik asit (2x1) ve suni gözyaşı başlanmıştır. Konjonktival sürüntü örneğinin mikroskobik incelemesinde polimorf nüveli lökositler görülmüş, ancak bakteri görülmemiştir. Kültürde saf olarak katalaz ve oksidaz pozitif, gram-negatif diplokok üremiştir. Üremeden sonra tekrar ilk Gram boyama preparatı değerlendirildiğinde, az sayıda

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[Genotypic identification and distribution patterns of Candida parapsilosis complex species (C.parapsilosis sensu stricto, C.metapsilosis and C.orthopsilosis) isolated from clinical samples]

Mikrobiyoloji bülteni, 2011

Candida parapsilosis, which has recently gained increasing importance, is the second most common ... more Candida parapsilosis, which has recently gained increasing importance, is the second most common fungal pathogen isolated from clinical specimens. C.parapsilosis strains exhibiting genetic heterogeneity were previously considered as a complex of three genetically different groups (group I, II, III). However, they have recently been reclassified as new species and named as C.parapsilosis sensu stricto (Grup I), C.orthopsilosis (Grup II) and C.metapsilosis (Grup III). In the present study we aimed to identify C.parapsilosis complex species by PCR-RFLP (Polymerase chain reaction-Restriction fragment lenght polymorphism) method and to determine the distribution of new species isolated from clinical specimens. A total of 68 samples (44 blood, 10 urine, 5 wound, 2 paracentesis fluids, 2 tympanocentesis samples and one of each cerebrospinal fluid, peritoneal fluid, surgical material, oral lesion and nail sample) in which C.parapsilosis had been isolated and identified with API 20C AUX (bio...

[Pandemic influenza A/H1N1/2009 virus RNA isolation rate in specimens of patients diagnosed as flu in a University Hospital in Eastern Black Sea Region, Turkey]

Mikrobiyoloji bülteni, 2010

Swine origin influenza virus (S-OIV) has been of global concern towards the end of 2009 with its ... more Swine origin influenza virus (S-OIV) has been of global concern towards the end of 2009 with its high morbidity rate and pandemic aspect. In this study, the presence of pandemic influenza A/H1N1/2009 virus RNA was investigated in patients clinically diagnosed as influenza infection in the university hospital in Trabzon province (located at Eastern Black Sea Region, Turkey). Oropharyngeal and nasal swab samples were collected from 211 patients (mean age: 18.5 years) who were admitted to our hospital between 16 November 2009 and 10 January 2010. Pandemic influenza A/H1N1/2009 virus RNA in the samples was investigated by real-time polymerase chain reaction. Viral RNA was detected in 41 of the patients (19.4%). The mean age of the cases was 11.7 years old. The highest positivity rate (44%) was seen in samples collected between 23-29 December 2009, while no positive samples were detected after 29 December 2009.

[Resistance rates of Mycobacterium tuberculosis complex strains isolated from clinical specimens]

Mikrobiyoloji bülteni, 2011

The aim of this study was to investigate isoniazid, rifampicin, streptomycin and ethambutol resis... more The aim of this study was to investigate isoniazid, rifampicin, streptomycin and ethambutol resistance rates of Mycobacterium tuberculosis complex strains isolated from clinical specimens in the Mycobacteriology Laboratories of Karadeniz Technical University Hospital between January 2005-March 2010. A total of 212 M.tuberculosis complex strains, 152 (71.7%) of which were isolated from respiratory specimens and 60 (28.3%) from non-respiratory system specimens, were included to the study. M.tuberculosis complex strains were determined by polymerase chain reaction using primers specific for the IS6110 gene region. Single isolate of each patient were enrolled in the study. Antituberculosis drug susceptibility testing was performed by BACTEC MGIT 960 (Becton Dickinson, USA). Of the 212 isolates, 157 (74.05%) were susceptible to all of the four antimycobacterial agents while 55 (25.9%) were found resistant to one or more of the drugs. The monodrug resistance rates were found as 6.1% for i...

Investigation of Pneumocystis jirovecii in Respiratory Samples of Immunocompromised Patients with PCR, IFA and Giemsa Staining Methods

Mikrobiyoloji Bulteni, 2013

Pneumocystis jirovecii is an important opportunistic agent leading to pneumonia in immunocompromi... more Pneumocystis jirovecii is an important opportunistic agent leading to pneumonia in immunocompromised patients. In this study, the presence of P.jirovecii were investigated by using Giemsa stain, indirect fluorescent antibody (IFA) test and two different nested polymerase chain reaction (nPCR) assays in respiratory samples obtained from 50 immunocompromised patients presenting with respiratory symptoms. The target genes used for nested PCR were mitochondrial large subunit ribosomal RNA (MtLSUrRNA) and internal transcribed spacer (ITS) region. P.jirovecii was detected in 7 (14%) and 11 (22%) respiratory samples by IFA and PCR, respectively, although all samples were negative with Giemsa stain. As a result, IFA and PCR were found to be rapid and reliable tests for the diagnosis of P.jirovecii infections and they should better be used together for accurate diagnosis.

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İmmünkompetan Bireylerde Cyclospora cayetanensis Enfeksiyonu

Mediterranean Journal of Infection Microbes and Antimicrobials, 2014

Cyclospora cayetanensis uzamış sulu ishal, karın ağrısı, iştahsızlık, halsizlik, bulantı gibi gas... more Cyclospora cayetanensis uzamış sulu ishal, karın ağrısı, iştahsızlık, halsizlik, bulantı gibi gastrointestinal semptomlarla seyreden s bir coccidian parazittir. Hastalık sıklıkla HIV/AIDS hastaları gibi immünsüpresif bireylerde görülmektedir. Asemptomatik seyredebildiği gibi, özellikle immünsüpresif konaklarda hayatı tehdit eden enfeksiyonlara da neden olabilmektedir. Hastalığın ayırıcı tanısı dikkatle yapılmalı, semptomatik olgularda uygun antibiyotik tedavisi mutlaka uygulanmalıdır. Dışkı incelemesinde C. cayetanensis kistlerinin s Cryptosporidium kistleriyle ayırıcı tanısında özellikle çok dikkatli olunmalıdır. Çalışmamızda 22 ve 80 yaşında m C. cayetanensis enfeksiyonu teşhis edilen semptomatik iki erkek olgu sunulmuştur. Olgularımızda tanı direkt mikroskopinin s yanında, dışkının modifiye Ziehl-Neelsen boyaması ve ultraviyole mikroskopisiyle konulmuştur. Her iki hastamızın da herhangi bir seyahat ve antibiyotik kullanım öyküsü mevcut olmayıp, genç olan olgumuzun fast-food yeme alışkanlığı mevcuttu. Olgularımızın dışkı kültürlerinde patojen bir bakteri üremesi olmadı. Serumlarında bakılan anti-HIV antikoru negatif bulundu. Olgularımız immün statü açısından normal olan bireylerdi. Semptomları uzun süredir devam eden olgumuz oral yoldan kullanılan bir haftalık trimetoprim-sülfametoksazol (TMP-SMZ) 2 x 160/800 mg/gün tedavisi ile başarıyla tedavi edilmiştir. Diğer olgumuzda da esas olarak selülit tedavisi amacıyla intravenöz (IV) başlanan moksifloksasin 1 x 400 mg/gün IV tedavisine olumlu yanıt alınmış ve bu tedaviye bir hafta süreyle devam edilmiştir. Olgularımızda klinik bulgulardaki düzelmeye paralel olarak yapılan kontrol mikroskopilerinde, dışkıda C. cayetanensis kistlerinin tamamen kaybolduğu görülmüştür. Her iki olgumuzun üç aylık takibinde s relaps görülmemiştir. İshal yakınması veya bulantı, karın ağrısı gibi diğer gastrointestinal sistem şikayetleri olan immünkompetan hastalarda da C. cayetanensis'in etken olabileceği düşünülmelidir. Uzun süreli semptomları olan immünkompetan bireyler de TMP-SMZ ile tedavi edilmelidir.

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Detection of Human Herpesvirus 6 DNA but not Human Herpesvirus 7 or 8 DNA in Atherosclerotic and Nonatherosclerotic Vascular Tissues

The Heart Surgery Forum, 2010

Introduction: Various viral infections are thought to play a role in the development of atheroscl... more Introduction: Various viral infections are thought to play a role in the development of atherosclerosis. A number of studies suggest that certain viruses from the Herpesviridae family in particular may lead to atherosclerosis. Methods: We investigated the presence of human herpesvirus 6 (HHV-6), human herpesvirus 7 (HHV-7), and human herpesvirus 8 (HHV-8) DNA in carotid, iliac, and coronary artery specimens obtained from a group of adult autopsy cases by means of polymerase chain reaction (PCR) analysis and nested PCR techniques. A 28-subject study group with at least type IV atherosclerosis and a 25-subject control group with no visible atherosclerosis were enrolled. Results: HHV-6 DNA was found in the carotid artery specimen of 1 subject with atherosclerosis, in an iliac artery specimen of another subject, and in the iliac artery specimen of one of the control subjects. HHV-7 or HHV-8 DNA was not found in either the atherosclerosis or control cases. Conclusions: This study is the fi rst to demonstrate the presence of HHV-6 in atherosclerotic vascular tissues. HHV-7 and HHV-8 were not found in atherosclerotic tissues; however, further research on broader study groups and with different protocols is needed to determine whether these viruses play a role in the formation of atherosclerosis.

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Does Cold Environment Affect Streptococcus pneumoniae Adherence to Rat Buccal Epithelium?

Respiration, 2006

Background: Colonization ofthe nasopharyngeal flora by Streptococcus pneumoniae increases in wint... more Background: Colonization ofthe nasopharyngeal flora by Streptococcus pneumoniae increases in winter. However, in vitro studies suggest that bacterial adherence decreases in low temperatures. These contras may be related to the difference between in vitro and physiological conditions. Objectives: We investigated whether pneumococcal adherence to buccal epithelial cells of rats increases in cold temperature. Methods:The adherence of S. pneumonia (ATCC 49619) was studied in six groups of rats. Basal pneumococcal adherence was checked after 1 h both at room temperature and in a cold environment (at 10°C) for the evaluation of changes in basal flora in the first two groups (basal control). In the remaining four groups, the effect of cold treatment before or after inoculation of S. pneumoniae, or cold treatment during the whole study period was investigated. Results: The adherence of inoculated pneumococci was found to be significantly decreased in rats exposed to cold air during the whol...

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Comparison of the sealing ability of three filling techniques in canals shaped with two different rotary systems: A bacterial leakage study

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2009

This study compared the sealing ability of 3 current filling techniques in root canals shaped wit... more This study compared the sealing ability of 3 current filling techniques in root canals shaped with 2 different rotary systems. Study design. Eighty human extracted mandibular premolars were divided randomly into 2 similar groups of 40 each and instrumented with either ProTaper (Dentsply Maillefer, Tulsa, OK; group A) or Mtwo (VDW; Antaeos, Munich, Germany; group B) rotary systems. Each group was divided into 3 subgroups (n ϭ 10) and 2 control groups (n ϭ 5). Canals were filled either with the tapered single-cone technique (SC; subgroups A1 and B1), with lateral condensation (LC; subgroups A2 and B2), or warm vertical compaction (WVC; subgroups A3 and B3). AH Plus was used as a root canal sealer in all groups. Samples were sterilized in an ethylene oxide sterilizer for 12 hours. The apical 3-4 mm of the roots were immersed in brain-heart infusion culture medium with phenol red indicator within culture chambers. The coronal access of each specimen was inoculated every 48 hours with a suspension of Enterococcus faecalis. Bacterial leakage was monitored every 24 hours for 8 weeks. The data obtained were analyzed using a chi-squared test, and P was set at .05. Results. In group A, 70% of the specimens filled with SC (subgroup A1), 50% of the specimens filled with LC (subgroup A2), and 20% of the specimens filled with WVC (subgroup A3) leaked. There was no statistically significant difference between the subgroups (P Ͼ .05). In group B, bacterial leakage was observed in 50% of SC samples (subgroup B1), 40% of LC samples (subgroup B2), and 50% of WVC samples (subgroup B3). There was no statistically significant difference between subgroups B1, B2, and B3 (P Ͼ .05). There was also no statistically significant difference between group A and group B (P Ͼ .05). Conclusion. Filling with SC, LC, and WVC techniques in canals treated with ProTaper or Mtwo rotary instruments showed similar levels of sealing efficacy. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:e129-e134)

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Effect of smear layer and root-end cavity thickness on apical sealing ability of MTA as a root-end filling material: A bacterial leakage study

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2010

The aim of this study was to investigate the effect of the cavity thickness and smear layer on ap... more The aim of this study was to investigate the effect of the cavity thickness and smear layer on apical sealing ability of mineral trioxide aggregate (MTA) as a root-end filling material. Study design. Seventy single-rooted maxillary central teeth were used in this study. All teeth were instrumented to size 50 using a step-back technique. The selected teeth were randomly divided into 4 groups, each containing 15 experimental samples and 5 positive and 5 negative control samples. In the first and second groups (smearϩ), the teeth were irrigated with only 5.25% NaOCl. In the third and fourth groups (smearϪ), the teeth were irrigated with 17% EDTA and 5.25% NaOCl to remove the smear layer. Also, in the first and third groups, cavities were prepared as 3 mm. In the second and fourth groups, cavities were prepared as 5 mm. All the root-end cavities were then filled with MTA. Nail varnish was applied to all external root surfaces to the level of the resected root-ends to prevent lateral microleakage. Samples were sterilized in an ethylene oxide sterilizer for 12 hours. The apical 3-4 mm of the roots were immersed in brain heart infusion culture medium with phenol red indicator within culture chambers. The coronal access of each specimen was inoculated every 48 hours with a suspension of Enterococcus faecalis. Bacterial leakage was monitored every 24 hours for 4 weeks. The data obtained were analyzed using a chi-squared test, with ␣ ϭ .05 as the level for statistical significance. Results. There were no statistically significant differences in rate of bacterial leakage among the experimental groups at 1-4 weeks (P Ͼ .05). Also, there was no difference between the groups when the 2 thickness groups were combined (P Ͼ .05). However, there was statistically significant differences when the 2 smear groups were combined for 4-week observation periods (P Ͻ .05). Removal of the smear layer caused significantly more apical microleakage than when the smear layer was left intact for 4 weeks. Conclusion. The thickness of root-end cavity (3 or 5 mm) had no influence in the bacterial leakage of the root end filled with MTA. Removing the smear layer may not be necessary in root-end cavities filled with MTA. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:e67-e72)

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CEP152 is a genome maintenance protein disrupted in Seckel syndrome

Nature Genetics, 2010

Functional impairment of DNA damage response pathways leads to increased genomic instability. Her... more Functional impairment of DNA damage response pathways leads to increased genomic instability. Here we describe the centrosomal protein CEP152 as a new regulator of genomic integrity and cellular response to DNA damage. Using homozygosity mapping and exome sequencing, we identified CEP152 mutations in Seckel syndrome and showed that impaired CEP152 function leads to accumulation of genomic defects resulting from replicative stress through enhanced activation of ATM signaling and increased H2AX phosphorylation.

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Direct and Transdentinal (Indirect) Antibacterial Activity of Commercially Available Dental Gel Formulations against Streptococcus mutans

Medical Principles and Practice, 2013

Objective: To evaluate the direct and transdentinal (indirect) agar diffusion antibacterial activ... more Objective: To evaluate the direct and transdentinal (indirect) agar diffusion antibacterial activity of different commercially available antibacterial dental gel formulations against Streptococcus mutans. Materials and Methods: The commercially available dental gel formulations were Corsodyl® (COG, 1% chlorhexidine), Cervitec® (CEG, 0.2% chlorhexidine + 0.2% sodium fluoride), Forever Bright® (FOB, aloe vera), Gengigel® (GEG, 0.2% hyaluronic acid), 35% phosphoric acid gel and distilled water (control). Direct agar diffusion was performed by isolating three wells from brain-heart infusion agar plates using sterile glass pipettes attached to a vacuum pump and adding 0.1 ml of the gels to each well. Transdentinal (indirect) agar diffusion was performed by applying gel to 0.2- and 0.5-mm-thick human dentin discs previously etched with phosphoric acid and rinsed with distilled water. Zones formed around the wells and the dentin discs were measured and analyzed using Kruskal-Wallis and Man...

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Ekstrakardiak aterosklerotik arter hastalıklarında Chlamydia pneumoniae'nin rolü

Türk Göğüs Kalp Damar Cerrahisi Dergisi, 2001