Papers by khodaberdi kalavi
Medical Laboratory Journal
There are several reports about the modulatory effects of intracellular pathogens, such as Leishm... more There are several reports about the modulatory effects of intracellular pathogens, such as Leishmania spp., to help survival and replication inside host cells. They alter host cells' defense and offence mechanisms, and in case of leishmaniasis, the pathogen creates a hostile environment inside macrophages. We performed an RNA sequencing analysis of transcriptome changes in Leishmania major-infected human macrophages at four hour post infection. In this study, we investigated gene expression pattern of the infected macrophages against microbead (4.16 µm) polystyrene particles phagocytized and non-polarized macrophages as controls. Monocytes with high purity were isolated from healthy donors by magnetic-activated cell sorting and then differentiated into macrophages after 6-9 days of incubation at 37 ˚C. In this study, we focused on some key interaction events between host cell and the pathogen; so some feature steps resulting from dictation strategies of the pathogen are presented below.
Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology
2016 IEEE Symposium on VLSI Circuits (VLSI-Circuits)
Low-power embedded memory and an ARM Cortex-M0 core that operate at 30 MHz were fabricated in com... more Low-power embedded memory and an ARM Cortex-M0 core that operate at 30 MHz were fabricated in combination with a 60-nm c-axis aligned crystalline indium-gallium-zinc oxide FET and a 65-nm Si CMOS. The embedded memory adopted a structure in which oxide semiconductor-based 1T1C cells are stacked on Si sense amplifiers. This memory achieved a standby power of 3 nW while retaining data and an active power of 11.7 μW/MHz by making each bitline as short as each sense amplifier. The M0 core adopted the flip-flop in which an oxide semiconductor-based 3T1C cell is stacked on the Si scan flip-flop cell without area overhead and achieved a standby power of 6 nW while retaining data. The combination of the embedded memory and the M0 core provided high-performance, low-power Internet of Things devices operating with a broad range of active standby power ratios.
Pakistan Journal of Biological Sciences, 2007
Cell Journal (Yakhteh), 2021
Objective Leishmaniasis is caused by members of the Leishmania species and constitute a group of ... more Objective Leishmaniasis is caused by members of the Leishmania species and constitute a group of infective diseases that range from cutaneous lesions to lethal visceral forms. In infected persons, macrophages recognize and eliminate the parasites via phagocytosis. In order to change a hostile environment into an environment adequate for survival and reproduction, the engulfed Leishmania species needs to modulate the function of its host macrophage. The expression patterns of cytokine genes such as interleukin-12 (IL-12), tumour necrosis factor-alpha (TNF-α), IL-1, and interferon-gamma (IFNγ) represent the immune response. In this study, we employed an RNA-seq approach for human monocyte-derived macrophages infected with Leishmania major (L. major) to decipher cytokine gene expression alterations in host macrophages. Materials and Methods In this descriptive study, human monocytes were isolated by magnetic activated cell sorting (MACS) and cultured in the presence of monocyte colony ...
International wound journal, 2010
Dear sirs Professor Paul Davis has in his article (1) considered advances and improvements in imm... more Dear sirs Professor Paul Davis has in his article (1) considered advances and improvements in immunology a major contribution towards the therapeutics of diseases, especially wound healing. We have been working along similar lines for more than a decade in drug design and have registered such drugs as patents (2–5). Several well-known documents indicate that cellular and molecular impairments of immunologic origin are seen in chronic wounds such as diabetic foot ulcers (DFUs) (6). They are cytokine defects (mainly Th-1 mediated), tendency to be associated with other chronic diseases [tuberculosis in diabetes mellitus (DM) (7)], overexpression of matrix metalloproteinases (MMPs) (1,8) etc. On the basis of worldwide literature, we have been working on reverting or correcting the defects responsible for DFU. We hypothesised that the defect could be corrected by repairing a biologic (immunologic based) mechanism that has been assumed to be impaired in patients with DFU and chronic wounds (6). To prove such a hypothesis, we proposed that factors such as decrease in macrophages and fibroblasts and other cell recruitment impairments and leukocyte function defects might have been defective in these patients. Disturbances in pro-inflammatory and inflammatory cytokines such as interleukin (IL)-1, tumour necrosis factor (TNF)/alpha, and others such as transforming growth factor alfa and beta (TGF), interleukine 8 (IL-8), granulocyte monocyte colony stimulating factor (GM-CSF), and interferon gamma (IFN/gamma) and many other vital cells and molecules were all indicative of an immunological defect in DFU. On the other hand, based on the nature of the abnormalities, we considered these patients to be relatively immunocompromised, especially with innate immune defects. We postulated that many signs in these patients indicate that they may lack proper cell immunity. Other studies had also shown this to be true. The immune defects included a lack of CD3+ T lymphocytes, macrophage recruitment defect, neutrophils and other phagocytic dysfunctions. Disturbances in neutrophil function such as failure of respiratory burst, INF/gamma, interleukin 12 (IL-12), and deficiency of IL-1, IL-2 receptors (IL-1 R, IL-2 Rs) and some other critical factors led us to confirm that these patients had innate cellular immunity failure, as already discussed in our hypothesis (6). We then suggested the possible importance of gaining the effector functions of some critical cells as phagocytes. In our subsequent works, we postulated that this can be achieved with Th-1 cell activation as the mechanism of cellular and molecular conduction. It could be concluded that one of the defects in DFU might be the nonspecific immunity in its cellular and molecular aspects. To achieve our goal in this direction we proposed that Th-1 cells are the major gateway through which the body may refresh itself. In this stage, we used activators of Th-1 cells as adjuvants. The non-specific immunity defects were previously described in some reports. Some interesting features observed apart from the healing of non-healing wounds using the available protocols are blockage of proteolytic activity of enzymes associated with clot formation as an appropriate mechanism for cells and molecules necessary to act on wound healing, autolytic debridement of necrotic lesions without use of surgical tools, control of infection without use of antibiotics and interestingly control of interdigital fungal infection resistant to all used antifungal agents.
Research Journal of Microbiology, 2007
Present survey basically focused on women between 15-45 years of age resident in a town of Sistan... more Present survey basically focused on women between 15-45 years of age resident in a town of Sistan and Baluchistan province named as Saravan city located in border of Pakistan-Iran in order to find out the seroposit:ivity against the viruses in child bearing ages in the above stated under study community. This descriptive cross-sectional study was carried-out from 2001 up to 2002. Saravan town was divided into 4 geographical areas and each area was further subdivided into 10 blocks and in each block 10 families were chosen randomly. In the next step by refening to each family from the chosen married women with specified age i.e., 15-45 years, 5 mL blood was collected. Serum was then separated and stored at-20°C before the assay. ELISA kit was employed to detect anti B19, anti rubella, anti measles, anti HBV and anti HCV antibody. Furthermore during samples collection a questionnaire filled for each woman llllder study. This study showed that 89 .6% of women llllderstudywere seropositive against measles, rubella (96.2%), B19 (59.2%), HCV (0.8%) and HBV (19 .8%), respectively. According to the results of no serious problem with rubella in this area; But, about measles, the present immunity against measles in this area is insufficient. It seems that incidence of B 19 infection in this region is same as other places in Iran. The rate of seropositivity against HBV and HCV indicated of these viruses circulating in the population in this area.
Journal of Gorgan University of Medical Sciences, 2009
Abbas Ali Keshtkar (PhD), Shahryar Semnani (MD), Gholamreza Roshandel (MD)* Maryam Aboomardani (P... more Abbas Ali Keshtkar (PhD), Shahryar Semnani (MD), Gholamreza Roshandel (MD)* Maryam Aboomardani (PhD) , Nafiseh Abdolahi (MD), Sima Besharat (MD) Abdolvahab Moradi (PhD), Khodaberdi Kalavi (MSc), Saba Besharat (BSc) Honeyeh Sadat Mirkarimi Assistant Professor, Department of Social Medicine, Gorgan University of Medical Sciences, Gorgan, Iran. Associate Professor, Department of Gastroenterology & Hepatology, Golestan Research Center of Gastroenterology & Hepatology, Gorgan University of Medical Sciences, Gorgan, Iran. General Physician, Golestan Research Center of Gastroenterology & Hepatology, Gorgan University of Medical Sciences, Gorgan, Iran. Nutritionist, Gorgan University of Medical Sciences, Gorgan, Iran. Assistant of Internal Medicine, Iran University of Medical Sciences, Tehran, Iran. Associate Professor, Department of Virology, Golestan Research Center of Gastroenterology & Hepatology, Gorgan University of Medical Sciences, Gorgan, Iran. Academic Instructor, Department of He...
Background and objectives: Human T-Lymphocyte Virus-1 (HTLV1) is known as the etiologic factor of... more Background and objectives: Human T-Lymphocyte Virus-1 (HTLV1) is known as the etiologic factor of acute T-Lymphocytic Leukemia (ATL) and tropical spastic paralysis. (TSP). Endemic factors causing infection with Human T Lymphocyte Virus-1 (HTLV-1) is based on environmental, socio-economical and health behaviors of the individuals. This virus is well distributed in families with involved members. Golestan province is located in North West part of Northern Khorasan province that had already been known as an endemic area for HTLV-1. This virus is also known as the main etiologic factor for cancers and ATL, therefore; we studied the prevalence of HTLV-1 seroepidemiology in Golestan province. Material and Methods: The subjects selected by cluster sampling were 2034 healthy cases residing in different parts of Golestan province. ELISA method using Diapro anti HTLV-1 antibody kits was applied for serological assessment. Western Blot (HTLV BLOT 2.4) was used for confirmation purposes. Result...
Iranian journal of cancer prevention, 2010
Background: Colorectal cancer (CRC) is the third common malignancy all over the world. Modern lif... more Background: Colorectal cancer (CRC) is the third common malignancy all over the world. Modern life styles affecting nutritional habits have been providing a potential impact on CRC. This study aims to assess the nutritional characteristics in CRC patients of Golestan province, Northeast of Iran. Methods: All incident CRC cases (N=47) in northeast of Iran (2004-2005) and a matched number of healthy persons were selected as cases and controls respectively. The subjects filled the Questionnaires containing socio-demographic and nutritional characteristics (FFQ). Results: Data analysis showed that Total Energy Expenditure (TEE) higher than 1837.5 Kcal/ day (first quartile of TEE) was significantly related to CRC risk (OR= 3.8; 95% Cl:l.2-ll.3); no other relationship was reported. Conclusions: Findings suggested that higher levels of total energy expenditure may increase the risk of CRC about 4-fold. We also found that there are relationships between CRC and high fat diets or cooking met...
Cell J. 2021; 22(4): 476-481., 2021
Objective: Leishmaniasis is caused by members of the Leishmania species and constitute a group of... more Objective: Leishmaniasis is caused by members of the Leishmania species and constitute a group of infective diseases that range from cutaneous lesions to lethal visceral forms. In infected persons, macrophages recognize and eliminate the parasites via phagocytosis. In order to change a hostile environment into an environment adequate for survival and reproduction, the engulfed Leishmania species needs to modulate the function of its host macrophage. The expression patterns of cytokine genes such as interleukin-12 (IL-12), tumour necrosis factor-alpha (TNF-α), IL-1, and interferon-gamma (IFNγ) represent the immune response. In this study, we employed an RNA-seq approach for human monocyte-derived macrophages infected with Leishmania major (L. major) to decipher cytokine gene expression alterations in host macrophages. Materials and Methods: In this descriptive study, human monocytes were isolated by magnetic activated cell sorting (MACS) and cultured in the presence of monocyte colon...
Turkish Journal of …, 2007
Present study was carried out in three years' duration (2002 2004) to investigate the risk factor... more Present study was carried out in three years' duration (2002 2004) to investigate the risk factors of esophageal cancer in Iranian Turkmens (Northeast of Iran; where the esophageal cancer is the most common world wide). Concerning regis tered cases of esophageal cancer in the region, 139 cases were selected. Controls matching in terms of age and sex were 500 healthy individuals. Furthermore, a questionnaire was designated with several questions including: personal characteristics, family history of cancer, possible disease background, and addiction to cigarettes or drugs, and presence of any particular dietary habits. The questionnaires filled with free will (voluntarily) by cases and controls. The collected data was analyzed by SPSS 10.0 software. The mean age of people under study was 60 years. The studied cases were significantly correlated with their first line relatives in term of cancer contraction (P=0.001 and OR=7.6). It was also significant in second and third line relatives (P=0.042 and OR=3.38). There was a significant correlation consid ering the consumption of stale food and butter with esoph ageal cancer. As a conclusion, in addition to mentioned risk factors, some other factors as mode of nourishment, em ployment status, special hygienic habits and inheritance were important factors in esophageal cancer etiology among the studied subjects.
Journal of Applied …, 2008
Iranian Journal of …, 2008
... Khodaberdi Kalavi1, Abdolvahab Moradi1, Abbas Ali Keshtkar1, Mergen Kalavi1, Golnaz ... 4 Bre... more ... Khodaberdi Kalavi1, Abdolvahab Moradi1, Abbas Ali Keshtkar1, Mergen Kalavi1, Golnaz ... 4 Brem H, Olivera Stojadinovic, Robert F Diegelmann, Hyacinth Entero, Brian Lee, Irena Pastar I, Golinko M, Rosenberg H, Tomic-Comic M. Molecular Markers in patients with chronic ...
Journal of Clinical and …, 2008
This study was designed to provide the clinical and epidemiological data on Inflammatory Bowel Di... more This study was designed to provide the clinical and epidemiological data on Inflammatory Bowel Disease (IBD) in the Golestan province (northeast of Iran). We carried out a retrospective study with regards to all IBD patients (pathologically confirmed cases) during 2001-2004. Registered cases were 108 [104 with Ulcerative Colitis (UC) and 4 with Crohn's disease (CD)]. Information about age, sex, education, colitis extent, extra intestinal manifestations, the lag between onset of the disease, and time of final diagnosis, were recruited. Data were analyzed using SPSS-V12 analytical software. There was a slight female predominance in UC cases. Most of the cases (83.3%) were highly educated, and 65.7% were living in urban areas. Patients with US mainly presented with diarrhoea, whereas those with CD complained of abdominal pain. The predominant form of UC was left sided colitis, which affected almost 35.4% of patients. The most extensive form (pan colitis) was present in 22.1%. Mean age was significantly different with regards to anatomical sites; patients with proctitis was younger than other groups (p =0.001). The mean lag time between the onset of symptoms and definite diagnosis was 2.2 1.1 and 5 months in UC and CD, respectively. Extra intestinal manifestations were seen in 10 patients. In the Golestan province, IBD is predominant in females, and its most severe form is higher than that documented in European studies.
International Wound Journal, 2010
Medical Laboratory Journal, 2015
Background and Objective: Normal hemoglobin (Hb) is formed of a heme group and a protein group kn... more Background and Objective: Normal hemoglobin (Hb) is formed of a heme group and a protein group known as globin. Globin is made of four polypeptide chains and in hemoglobinopathies, the structure of one of these four polypeptide chain becomes abnormal. Cellulose acetate method is a common way to differentiate haemoglobinopathies. Inability to identify the components of Hb low concentrations and incapability to isolate all Hb types are among the disadvantages of this method. The aim of this study was to report the prevalence of hemoglobinopathies in the North of Iran by capillary electrophoresis method. Methods: All patients with suspected hemoglobinopathies, referred by physicians for electrophoresis, have been studied in a private center in the city of Gorgan, Iran. The level of HbA2, HbA, HbF and other Hb was recorded. Results: Overall, 725 blood samples were analyzed using the capillary method. HbE was reported in 2 patients, HbH was observed in 2 patients and Hb Barts was reported in 3 patients. Using the capillary method, among patients with the SDG area, only 4 of 38 (10.52%) had HbS and the majority of them (89.48%) had HbD. Conclusion: HbD is the most common hemoglobinopathy in the North of Iran.
Annals of Saudi medicine
The aim of the study was to determine the status of low-density lipoprotein cholesterol (LDL-C) a... more The aim of the study was to determine the status of low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in human subjects during Ramadan fasting. Fasting during Ramadan (one month of food and water intake restricted only to night hours) is a religious obligation for Muslims. There are biochemical effects of changes in lifestyle during Ramadan. This report is a study of the effects of Ramadan fasting on the serum LDL-cholesterol and HDL-cholesterol concentrations. The study group consisted of 83 volunteers comprising 57 males (aged 21-55, mean 34.25+/-9.81 years) and 26 females (aged 20-58, mean 34.58+/-8.94 years). A regimen of one month of food and water intake restricted only to night hours was followed by the subjects. Subjects were evaluated one week before Ramadan fasting (pre-RF), two weeks after the start of Ramadan fasting (mid-RF), and at the fourth week of Ramadan fasting (end-RF). Serum specimens were obtained from subjects during ...
Iranian journal of basic medical sciences, 2013
Human T-cell lymphotropic virus type-1 is an oncornavirus that causes adult T cell leukemia (ATL)... more Human T-cell lymphotropic virus type-1 is an oncornavirus that causes adult T cell leukemia (ATL) HTLV-I-associated myelopathy⁄tropical spastic paraparesis (HAM/TSP). Golestan province is located in North West of Khorasan province known as an endemic area for HTLV-I in Iran. This study aimed to evaluate seroprevalence of HTLV-I in Golestan province. In this cross-sectional descriptive study in 2007, blood samples were collected from 2034 healthy people residing in different parts of Golestan province. Sera were assessed for HTLV-I/II-specific antibodies by ELISA method and reactive samples were confirmed by Western blot. Demographic and serologic data were entered in SPSS version 11.5 and statistical analysis was performed. An overall HTLV-I/II prevalence of 0.7% was observed in 15 cases by ELISA. Six out of 15 were confirmed as HTLV-I by western blot. Regional variation in the prevalence of HTLV-I was observed; 0%, 0%, 0.1%, 1.9%, 0.3%, 0%, and 2.6% tested HTLV-I-positive from west...
Uploads
Papers by khodaberdi kalavi