Papers by Mourad kharbach
journal of applied pharmaceutical science, 2023
The bioanalytical method certifications (validation) are very essential for therapeutic drug moni... more The bioanalytical method certifications (validation) are very essential for therapeutic drug monitoring. It is critical to use well-defined and completely validated procedures to provide reliable results which can be properly interpreted. This review provides practical steps and procedures with bioanalytical methods validation and highlights chromatographic method validations that have been used in the bioanalysis. It also attempts to clarify the nomenclature of certain stages of bioanalytical validation and to describe the overall process used for the drugs measurement and their metabolites in biological matrices and fluids. The stages of this procedure are discussed and correlated with data of scientific literature. A consistent description of key bioanalytical parameters is discussed and investigated with a view to improve scientific standards of bioanalytical methods used in clinical studies.
Agronomy, Aug 11, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of basic and clinical physiology and pharmacology, Dec 20, 2018
Background: Based on our previous ethnobotanical survey, the non-investigated Saharan plant Anaba... more Background: Based on our previous ethnobotanical survey, the non-investigated Saharan plant Anabasis aretioides Coss. & Moq., growing in the region of Errachidia, was selected for pharmacological investigation. In Moroccan traditional medicine, A. aretioides is being used for diabetes treatment. Thus, the current work aims at evaluating the antidiabetic, antioxidant, and antibacterial activities of the plant in relation to the digestive tract. Methods: The different parts of the plant (aerial parts, roots, seeds) were extracted with methanol (MeOH) and screened in enzymatic assays for their inhibitory potential against α-amylase and α-glucosidase, as well as antioxidant and antibacterial activities. Furthermore, the phenolic compounds were analyzed using HPLC-DAD-QTOF-MS. Results: The MeOH extracts of A. aretioides aerial parts, roots, and seeds, respectively, inhibited α-amylase (IC 50 of 3148.07 μg/mL, 2440.20 μg/mL, 3395.71 μg/mL) and α-glucosidase (IC 50 of 2940.59 μg/mL, 3521.81 μg/mL, 3393.83 μg/mL). Moreover, compared to aerial parts and seeds, the plant roots exhibited higher antioxidant capacity and a potent reducing power. In resazurin microplate assay, the plant parts displayed a minimal inhibitory concentration (MIC) ranging from 7.81 mg/mL to 31.25 mg/mL. Chemical analysis revealed 25 phenolic compounds, with chlorogenic acid as the main phenolic compound in the aerial parts, hesperidin in roots, and quercitrin in seeds. Conclusion: Anabasis aretioides cited for treatment of diabetes shows promising antioxidant and antibacterial properties, as well as an ability to inhibit digestive enzyme, including α-amylase and α-glucosidase. Thus, our results explain in part the traditional use of this Saharan medicine and open doors for further in vivo mechanistic and functional studies.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Foods
The globalization of the food market has created a pressing need for food producers to meet the e... more The globalization of the food market has created a pressing need for food producers to meet the ever-increasing demands of consumers while ensuring adherence to stringent food safety and quality standards [...]
Foods
In today’s era of increased food consumption, consumers have become more demanding in terms of sa... more In today’s era of increased food consumption, consumers have become more demanding in terms of safety and the quality of products they consume. As a result, food authorities are closely monitoring the food industry to ensure that products meet the required standards of quality. The analysis of food properties encompasses various aspects, including chemical and physical descriptions, sensory assessments, authenticity, traceability, processing, crop production, storage conditions, and microbial and contaminant levels. Traditionally, the analysis of food properties has relied on conventional analytical techniques. However, these methods often involve destructive processes, which are laborious, time-consuming, expensive, and environmentally harmful. In contrast, advanced spectroscopic techniques offer a promising alternative. Spectroscopic methods such as hyperspectral and multispectral imaging, NMR, Raman, IR, UV, visible, fluorescence, and X-ray-based methods provide rapid, non-destru...
Molecules
In addition to the nutritional and therapeutic benefits, Argan oil is praised for its unique bio-... more In addition to the nutritional and therapeutic benefits, Argan oil is praised for its unique bio-ecological and botanic interest. It has been used for centuries to treat cardiovascular issues, diabetes, and skin infections, as well as for its anti-inflammatory and antiproliferative properties. Argan oil is widely commercialized as a result of these characteristics. However, falsifiers deliberately blend Argan oil with cheaper vegetable oils to make economic profits. This reduces the quality and might result in health issues for consumers. Analytical techniques that are rapid, precise, and accurate are employed to monitor its quality, safety, and authenticity. This review provides a comprehensive overview of studies on the quality assessment of Moroccan Argan oil using both untargeted and targeted approaches. To extract relevant information on quality and adulteration, the analytical data are coupled with chemometric techniques.
19th Forum of Pharmaceutical Sciences, 2016
Conférence Chimiométrie 2020, 2020
16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology, 2020
Infrared Physics & Technology, 2022
Supplemental material, Supplementary Material 1, for HPLC-DAD/TOF-MS Chemical Compounds Analysis ... more Supplemental material, Supplementary Material 1, for HPLC-DAD/TOF-MS Chemical Compounds Analysis and Evaluation of Antibacterial Activity of <i>Aristolochia longa</i> Root Extracts by Nasreddine El Omari, Sanae Akkaoui, Omar El Blidi, Rokia Ghchime, Abdelhakim Bouyahya, Mourad Kharbach, Maâmar Yagoubi, Abdelaali Balahbib, Omar Chokairi and Malika Barkiyou in Natural Product Communications
SN Applied Sciences, 2021
Despite the richness and biodiversity of invertebrates and algae in the Mediterranean Sea, these ... more Despite the richness and biodiversity of invertebrates and algae in the Mediterranean Sea, these organisms are still poorly studied. The objective of our research is the discovery of bioactive lead compounds from the Mediterranean Sea sponge Hemimycale Collumella (HC). HC sponge (189.0 g) was collected from Mdiq costs on the Mediterranean Sea and extracted with methanol to yield (10 g) which was then subjected to fractionation. A bio-guided protocol was applied through evaluation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and Oxygen Radical Absorbance Capacity (ORAC), α-amylase, β-glucosidase, pancreatic lipase inhibition as well as anti-collagenase, anti-elastase, antityrosinase and cytotoxic activity. 2,3-O-Hexahydroxydiphenoyl-(α/β)-glucose (1) and gentisic acid 2-O-β-glucoside (2) were isolated from the water fraction, quercetin-3-O-β-glucopyranoside (3), kaempferol 3-O-β-glucopyranoside (4) and isorhamnetin 3-O-β-glucopyranoside (5) from n-butanol fraction, gallic acid ...
Agronomy, 2021
Tomato is an important economic crop that is widely consumed worldwide. Tomato production is main... more Tomato is an important economic crop that is widely consumed worldwide. Tomato production is mainly limited by the use of nitrogen fertilizer, sunlight, soil and water conditions. Biochar is one of the soil amendments, and it is recognized as a promising practice for improving crop production in agriculture. The effect of biochar on the photosynthetic traits and tomato yield under reduced nitrogen fertilizer application is still not well understood. The objective of this research is to investigate the influence of biochar application on the photosynthesis and yield of tomato under reduced nitrogen fertilizer application from the perspectives of the nutrient uptake of plants (nitrogen and phosphorus), leaf photosynthetic pigment and leaf gas exchange parameters. Two-year greenhouse experiments containing six biochar levels (0, 10, 30, 50, 70, and 90 t ha−1) and two nitrogen fertilizer application rates (190 and 250 kg ha−1) were conducted. Compared with C0, C50 significantly improved...
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Papers by Mourad kharbach