Papers by maria kalaitzidou
Journal of Biological Research-Thessaloniki, 2021
Background Microcystins are emerging marine biotoxins, produced by potentially toxic cyanobacteri... more Background Microcystins are emerging marine biotoxins, produced by potentially toxic cyanobacteria. Their presence has been reported in aquatic animals in Greek freshwater, while data are few in marine environments. Since the climate change induces eutrophication and harmful algal blooms in coastal marine ecosystems affecting the public health, further research on microcystins’ presence in marine waters is required. The aim of this study was to examine the potential presence of microcystins in mussels Mytilus galloprovincialis in the largest farming areas in Thermaikos gulf, in Northern Greece, and to investigate their temporal and spatial distribution, adding to the knowledge of microcystins presence in Greek Mediterranean mussels. Results A 4-year microcystins’ assessment was conducted from 2013 to 2016, in farmed Mediterranean mussels M. galloprovincialis, in five sampling areas in Thermaikos gulf, in northern Greece, where the 90% of the Greek mussels’ farming activities is loca...
Acta Veterinaria Eurasia, 2021
The effect of microcystins (MCs) was assessed on Mediterranean mussels Mytilus galloprovincialis ... more The effect of microcystins (MCs) was assessed on Mediterranean mussels Mytilus galloprovincialis during experimental exposure to marine cyanobacteria belonging to the genus Synechocystis. The strain was isolated for the first time in Greece, from the Thermaikos gulf (Central Macedonia, Northern Greece). All mussels used for the experiment were collected from farms located in the mentioned area. No toxic algae or phytoplankton populations were observed during the experimental period. The mussels were divided in three different groups; two of the three groups were fed daily with 10,000 cells/mL and 100,000 cells/mL of Synechocystis sp., respectively , whereas the third group was not fed at all. MCs were detected using a specific, validated direct competitive enzyme-linked immunosorbent assay. The experimental exposure was conducted in triplicates. The results were confirmed by ultra-high-performance liquid chromatography-tandem mass spectrometry. It was demonstrated that the Mediterranean mussels M. galloprovincialis could accumulate MCs up to 6.85±0.220 μg/kg after 72 hours of exposure at a density of 100,000 cells/mL to the marine strain Synechocystis sp.
The presence of toxic marine cyanobacteria and secondary metabolites, microcystins, were studied ... more The presence of toxic marine cyanobacteria and secondary metabolites, microcystins, were studied in Thermaikos Gulf in Central Macedonia in Greece, during the period from March 2013 to March 2014. Toxic marine cyanobacteria were isolated in marine agar and identified with PCR using primers based on 16S rDNA. The presence of microcystins in water extraction was detected by immunoassay (competitive ELISA). The concentration was ranged from 0.15 to 5ppm. It was observed that populations of toxic marine cyanobacteria were increasing during spring and early winter and there was a correlation to the physical and chemical parameters of the water. The percentage of microcystins was 20.8 % and there were significant differences (p<0.05) between the areas and the seasons.
Background Microcystins are emerging marine biotoxins, produced by potentially toxic cyanobacteri... more Background Microcystins are emerging marine biotoxins, produced by potentially toxic cyanobacteria. Their presence has been reported in aquatic animals in Greek internal waters, while data are few in marine environments. Since the climate change induce eutrophication and harmful algal blooms in coastal marine ecosystems affecting the public health, further research on microcystins’ presence in marine waters is required. The aim of this study was to detect the potential presence of microcystins in mussels Mytilus galloprovincialis in the largest farming areas in Thermaikos gulf, in Northern Greece, and to investigate their temporal and spatial distribution, adding to the knowledge of microcystins presence in Greek Mediterranean mussels. Results A three years microcystins’ assessment was conducted from 2013 to 2016, in farmed Mediterranean mussels Mytilus galloprovincialis, in five sampling areas in Thermaikos gulf, in northern Greece, where the 90% of mussels’ farming activities is l...
Acyanobacterial harmful algal bloom (CyanoHAB) has occurred in May 2015 in the Delta of Axios Riv... more Acyanobacterial harmful algal bloom (CyanoHAB) has occurred in May 2015 in the Delta of Axios River, in Thessaloniki, in the region of Central Macedonia, in Northern Greece. The bloom expanded to the coastline of Thermaikos Gulf near the areas of Chalastra and Kimina. Toxic Anabaena sp. was isolated from the water samples. Microcystin-RR(50ppb) was confirmed with immunoassay method for microcystins (Adda specific ELISA) and Liquid ChromatographyMass Spectrometry (LC-MS).In this area monitoring of microcystins and its algal blooms are only experimentally applied by a satellite system. This research investigated the toxin and microcystin cells in the local coastal water and emphasize to the satellite remote sensing, as a precaution, to reduce and avoid impacts on animals’ health, Public Health and ecosystems, after harmful algal blooms.
Environmental Microbiology, 2021
Global warming affects the aquatic ecosystems, accelerating pathogenic microorganisms' and to... more Global warming affects the aquatic ecosystems, accelerating pathogenic microorganisms' and toxic microalgae's growth and spread in marine habitats, and in bivalve molluscs. New parasite invasions are directly linked to oceanic warming. Consumption of pathogen-infected molluscs impacts human health at different rates, depending, inter alia, on the bacteria taxa. It is therefore necessary to monitor microbiological and chemical contamination of food. Many global cases of poisoning from bivalve consumption can be traced back to Mediterranean regions. This paper's aim is to examine the marine bivalve's infestation rate within the scope of climate change, as well as to evaluate the risk posed by climate change to bivalve welfare and public health. Biological and climatic data literature review was performed from international scientific sources, Greek authorities and State organizations. Focusing on Greek aquaculture and bivalve fisheries, high risk index pathogenic parasites and microalgae were observed during summer months, particularly in Thermaikos Gulf. Considering the climate models that predict further temperature increases, it seems that marine organisms will be subjected in the long-term to higher temperatures. Due to the positive linkage between temperature and microbial load, the marine areas most affected by this phenomenon are characterized as "high risk" for consumer health. This article is protected by copyright. All rights reserved.
Air & Water Borne Diseases, 2016
Cyanobacteria l Toxins Toxic Cyanobacteria produce secondary metabolites, the cyanotoxins. The cy... more Cyanobacteria l Toxins Toxic Cyanobacteria produce secondary metabolites, the cyanotoxins. The cyanotoxins are classified according to their chemical composition and structure in cyclic peptides, alkaloids and lipopolysaccharides and according to their toxicity in hepatotoxins, neurotoxins and cytotoxins [19]. Microcystins (MCYST) and Nodularins (NODLN) are the most widespread cyanotoxins. They are composed non-ribosomal by a
European Psychologist, 2003
Subjective quality of life can be defined in terms of life satisfaction (LS), subjective well-bei... more Subjective quality of life can be defined in terms of life satisfaction (LS), subjective well-being (SWB), happiness, and morale. In our study, we investigated the effect of demographic, health, affective, and adaptation-to-old-age factors on the above attributes of subjective quality of life. The sample was comprised of 160 elderly of both genders, aged 63 to 100 years. Half of them lived in a Greek city and the rest came from a small town and nearby villages. Participants filled in questionnaires on (a) demographic information; (b) subjective perception of their health condition; (c) emotional state; (d) adaptation to old age; (e) Life Satisfaction Index A and B ( Neugarten, Havighurst, & Tobin, 1961 ). Pearson correlation and regression analyses showed that the effect of demographic and health factors was mediated by affect and adaptation-to-old-age factors. Positive affect contributed to all aspects of subjective quality of life, whereas negative affect was important for the det...
European Psychologist, Dec 1, 2003
Amado, João 160 Avero, P. 252 Becker, Stefanie 168 Berdullas, Manuel 54 Bertnikov, Kirill A. 238 ... more Amado, João 160 Avero, P. 252 Becker, Stefanie 168 Berdullas, Manuel 54 Bertnikov, Kirill A. 238 Burmedi, David 168 Caprara, Gian Vittorio 131 Caprara, Mariagiovanna 129, 131 Callahan, Inés 101 Calvo, Manuel G. 252 Castillo, M. Dolores 252 Chankin, Grace 178 Cimino, Guido 23 Daatland, Svein Olav 200 Dahl, Tove I. 48 Efklides, Anastasia 178 Fernández-Ballesteros, Rocío 129, 148 Ferreri, Antonio 23 Fonseca, António M. 160 Foster, Jonathan K. 39 Frey, Dieter 92 ... Frischer, Josef 34 Furnham, Adrian 101 Gimeno, Luis ...
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Papers by maria kalaitzidou