Papers by kesia Palma Rigo
Journal of Developmental Origins of Health and Disease
Protein restriction during early phases of body development, such as intrauterine life can favor ... more Protein restriction during early phases of body development, such as intrauterine life can favor the development of vascular disorders. However, it is not known if peripubertal protein restriction can favor vascular dysfunction in adulthood. The present study aimed to evaluated whether a protein restriction diet during peripubertal period favors endothelial dysfunction in adulthood. Male Wistar rats from postnatal day (PND) 30 until 60 received a diet with either 23% protein (CTR group) or with 4% protein (LP group). At PND 120, the thoracic aorta reactivity to phenylephrine, acetylcholine, and sodium nitroprusside was evaluated in the presence or absence of: endothelium, indomethacin, apocynin and tempol. The maximum response (Rmax) and pD2 (-log of the concentration of the drug that causes 50% of the Rmax) were calculated. The lipid peroxidation and catalase activity were also evaluated in the aorta. The data were analyzed by ANOVA (one or two-ways and Tukey’s) or independent t-te...
Arquivos do Mudi
Methylphenidate (MPH) is the active ingredient in Ritalin, although effective in treating ofthe a... more Methylphenidate (MPH) is the active ingredient in Ritalin, although effective in treating ofthe attention deficit hyperactivity disorder (ADHD), its neurostimulation action is attractiveamong students in order to increase the focus or improving student performance. In context ofthe Developmental Origins of Health and Disease (DOHaD) concept, states that insults incritical phases of development, as the peripubertal period, may program the organism todysfunctions in adulthood. The aim of this work was to investigate the impact of peripubertalMPH exposure on learning and retrograde memory in adult rats. Male Wistar rats wereexposed to MPH (5 mg/kg) from post-natal day (PN) 30 until PN60 (MPH). Control animalsreceived 0.9% saline (Sal). At PN60, learning was evaluated true 10 days in an eight-armelevated maze and at PN120 retrograde memory. At PN60, MPH group showed a delay of61% in the latency, an increase of 100% in the number of errors and 200% in the number ofrepetition errors when ...
Hypertension, 1982
Renin-angiotensin and sympathetic nervous system activity were evaluated in grade school children... more Renin-angiotensin and sympathetic nervous system activity were evaluated in grade school children selected from the upper 0.26% and lower 5% of the blood pressure distribution constructed from a survey in the Minneapolis Public Schools. Eleven children from the upper 0.26% group and 19 children from the lower 5% group were admitted to the Clinical Research Center for 5 days and maintained on a 110 mEq sodium and 75 mEq potassium diet. On the fifth hospital day blood samples were obtained supine, after 2 hours of upright posture and after treadmill exercise. Mean sodium and potassium excretion and serum sodium and potassium were similar between the two groups. Plasma norepinephrine was not significantly different between the two groups at any of the three sampling times. Plasma renin activity was significantly lower in the upper 0.26% group in the supine and 2-hour upright samples. Mean plasma aldosterone (measured only in the supine blood samples) was not significantly different between groups. Plasma aldosterone values were significantly correlated with plasma renin activity only in the lower 5% group (r = 0.67, p < 0.005). This study suggests that in grade school children sympathetic nervous system activity is similar between children with high and low blood pressure but that plasma renin activity is lower and an apparent dissociation between plasma aldosterone and renin activity exists in the high blood pressure group. These findings should be confirmed in studies with larger numbers of subjects selected from the entire distribution of blood pressure. (Hypertension 4: 299-306, 1982) KEY WORDS • renin-angiotensin system childhood blood pressure norepinephrine • aldosterone • hypertension D URING the past decade, a growing interest in childhood hypertension has culminated in the publication of a number of epidemiologic surveys of blood pressure in children and adolescents. 1 These studies and others strongly suggest that factors influencing the development of essential hypertension extend into the first decade of life,'" 4 even to infant years,*"' and that levels of blood pressure associated with significant risk for cardiovascular morbidity in adults are also found within the pediatric age group." Thus, considerable support exists to warrant investi-From the
Frontiers in Physiology, Apr 28, 2022
arousal, which may be associated with a disorganization of brain and cardiac redox state and stru... more arousal, which may be associated with a disorganization of brain and cardiac redox state and structural cardiac alteration.
Saúde e Pesquisa, 2021
We investigated scientific publications on the prescription pattern of systemic hypertension drug... more We investigated scientific publications on the prescription pattern of systemic hypertension drugs and use of guidelines in primary health care by systematic review and meta-analysis. Articles were selected in the PubMed, Web of Science and LILACS databases, according to the PRISMA statements, from 2004 to 2020. The systematic review showed a higher prescription pattern for combination therapy (52,9%). The meta-analysis confirmed the superiority of prescription for combination therapy (OR 1.76, CI 1.29 - 2.41). Was observed higher monotherapy prevalence in the Swedish study (98%) and combined therapy in Nigerian (98%). Higher frequency prescriptive of angiotensin-converting enzyme inhibitors in Trinidad (64%); diuretics (64%), beta blockers (63%), and calcium channel blockers (53%) in Nigeria; and angiotensin-receptor blockers (43%) in Portugal. Regarding the use of guidelines, 50% the studies reported their use for the prescription of antihypertensive in primary health care.
Journal of Endocrinology, 2021
We tested whether chronic supplementation with soy isoflavones could modulate insulin secretion l... more We tested whether chronic supplementation with soy isoflavones could modulate insulin secretion levels and subsequent recovery of pancreatic islet function as well as prevent metabolic dysfunction induced by early overfeeding in adult male rats. Wistar rats raised in small litters (SL, three pups/dam) and normal litters (NL, nine pups/dam) were used as models of early overfeeding and normal feeding, respectively. At 30 to 90 days old, animals in the SL and NL groups received either soy isoflavones extract (ISO) or water (W) gavage serving as controls. At 90 days old, body weight, visceral fat deposits, glycemia, insulinemia were evaluated. Glucose-insulin homeostasis and pancreatic-islet insulinotropic response were also determined. The early life overnutrition induced by small litter displayed metabolic dysfunction, glucose, and insulin homeostasis disruption in adult rats. However, adult SL rats treated with soy isoflavones showed improvement in glucose tolerance, insulin sensitiv...
1Academica do Curso de Nutrição, Centro Universitário de Maringá – UNICESUMAR. Bolsista PROBIC-Un... more 1Academica do Curso de Nutrição, Centro Universitário de Maringá – UNICESUMAR. Bolsista PROBIC-UniCesumar. [email protected] 2Pós-graduanda, Mestre, doutoranda no Programa de pós-graduação em Ciências Biológicas da Universidade Estadual de Maringá – UEM. [email protected] 3Co-orientadora, PhD, professora do programa de pós-graduação em Fisiopatologia da Universidade Estadual de Maringá – UEM. [email protected] 4Orientador, PhD, professor do Centro Universitário de Maringá – UNICESUMAR. Pesquisador do Instituto Cesumar de Ciência, Tecnologia e Inovação (ICETI). [email protected]
International Journal of Obesity, 2021
Background Early postnatal overfeeding (PO) induces long-term overweight and reduces brown adipos... more Background Early postnatal overfeeding (PO) induces long-term overweight and reduces brown adipose tissue (BAT) thermogenesis. Exercise has been suggested as a possible intervention to increase BAT function. In this study, we investigated chronical effects of moderate-intensity exercise in BAT function in postnatal overfed male Wistar rats Methods Litters’ delivery was on postnatal-day 0 - PN0. At PN2, litters were adjusted to nine (normal litter – NL) or three pups (small litter – SL) per dam. Animals were weaned on PN21 and in PN30 randomly divided into sedentary (NL-Sed and SL-Sed) or exercised (NL-Exe and SL-Exe), N of 14 litters per group. Exercise protocol started (PN30) with an effort test; training sessions were performed three times weekly at 60% of the VO 2max achieved in effort test, until PN80. On PN81, a temperature transponder was implanted beneath the interscapular BAT, whose temperature was assessed in periods of lights-on and -off from PN87 to PN90. Sympathetic nerve activation of BAT was registered at PN90. Animals were euthanized at PN91 and tissues collected Results PO impaired BAT thermogenesis in lights-on ( p PO < 0.0001) and -off ( p PO < 0.01). Exercise increased BAT temperature in lights-on ( p Exe < 0.0001). In NL-Exe, increased BAT activity was associated with higher sympathetic activity ( p Exe < 0.05), β3-AR ( p Exe < 0.001), and UCP1 ( p Exe < 0.001) content. In SL-Exe, increasing BAT thermogenesis is driven by a combination of tissue morphology remodeling ( p Exe < 0.0001) with greater effect in increasing UCP1 ( p Exe < 0.001) and increased β3-AR ( p Exe < 0.001) content. Conclusion Moderate exercise chronically increased BAT thermogenesis in both, NL and SL groups. In NL-Exe by increasing Sympathetic activity, and in SL-Exe by a combination of increased β3-AR and UCP1 content with morphologic remodeling of BAT. Chronically increasing BAT thermogenesis in obese subjects may lead to higher overall energy expenditure, favoring the reduction of obesity and related comorbidities.
Journal of Developmental Origins of Health and Disease, 2021
Exercise counteracts obesity effects, but information on how early-life obesity may affect long-t... more Exercise counteracts obesity effects, but information on how early-life obesity may affect long-term adaptation to exercise is lacking. This study investigates the impact of early-life postnatal overfeeding (PO) on animals’ adaptation to exercise. Only male Wistar rats were used. On postnatal day (PN) 30, rats from control (NL-9 pups) or PO (SL-3 pups) litters were separated into four groups: NL-sedentary (NL-Se), NL-exercised (NL-Ex), SL-sedentary (SL-Se), and SL-exercised (SL-Ex). Exercised groups performed moderate-intensity exercise, running on a treadmill, from PN30 to PN90. Further experiments were carried out between PN90 and PN92. PO promoted obesity in SL versus NL rats (P < 0.05). Exercise reduced body weight (P < 0.001), body fat (P < 0.01), and improved glucose homeostasis in SL-Ex versus SL-Se. SL-Ex presented lower VO2max (P < 0.01) and higher post-exercise LDH (P < 0.05) compared to NL-Ex rats. Although moderate exercise counteracted obesity in SL rats,...
Toxicology and Applied Pharmacology, 2021
Metformin (Met) is widely used to control blood glucose levels and acts on various organs, includ... more Metformin (Met) is widely used to control blood glucose levels and acts on various organs, including reproductive tissues, to improve reproductive and lifespan. This study evaluated whether neonatal Met exposure prevented male reproductive dysfunction caused by being overweight during adulthood. Randomized Wistar rat pups received an intraperitoneal injection from postnatal days (PNDs) 1 to 12of saline (Sal; 0.9% NaCl/day in 2mL/kg) or Met (100 mg/kg/day in 2 mL/kg). From PNDs 60 to 90, the animals received a regular (R; 4.5% fat; Sal R and Met R groups) or a high-fat (HF; 35% fat; Sal HF and Met HF groups) diet. At PND 90, all animals were euthanized to evaluate their biometric and reproductive parameters. The Sal and Met groups with R showed similar body weights, however, the HF diet increased the body weight in both groups. The Sal HF group showed testicular damage regarding in antioxidant status and inflammatory profile in the epididymal cauda. The HF diet reduced Leydig and Sertoli cells numbers, with lower sperm quality. The Met R animals showed positive reproductive programming, due to improved antioxidant defense, inflammatory biomarkers, and sperm morphology. Met HF prevented HF diet damage to reproductive organs and sperm morphology, but not to sperm motility. Early Met exposure positively affected the male reproductive system of adult rats, preventing reproductive HF disorders. STATEMENT OF NOVELTY AND SIGNIFICANCE: Metformin is used to control type 2 diabetes mellitus and can act to improve metabolism and lifespan. Metformin avoidance is recommended during pregnancy, but there is no information regarding its use when breastfeeding. For the first time, we showed in this current study that metformin positively acts in the male reproductive tissues and helps involved in later life. These data showed a better antioxidant defense and anti-inflammatory profile of Metformin animals than Saline animals and might directly improve reproductive organs morphophysiology and sperm morphology. Also, the neonatal Met application programs the male reproduction to counterbalance damages from an obesogenic environment in later life.
Frontiers in Physiology, 2019
We aimed to test whether moderate physical training can induce long-lasting protection against ca... more We aimed to test whether moderate physical training can induce long-lasting protection against cardiovascular risk factors induced by high fat diet (HFD) intake, even after cessation of training. 90-days-old Wistar rats were submitted to a sedentary lifestyle or moderate physical training, three times a week, for 30 days. Following this, at 120 days-of age, sedentary and trained rats received a hypercaloric diet (HFD) or a commercial diet normal fat diet (NFD) for 30 days. Body weight (BW) and food intake were evaluated weekly. At 150 days-of age, hemodynamic measures (systolic, diastolic, mean blood pressure, pulse pressure, pulse interval and heart rate) were made via an indwelling femoral artery catheter. Beat-to-beat data were analyzed to calculate power spectra of systolic blood pressure (SBP) and pulse interval. After euthanasia, mesenteric fat pads were removed and weighted and total blood was stored for later analysis of lipid profile. Consumption of a HFD increased blood pressure (BP), pulse pressure, low frequency BP variability, BW gain, fat pad stores and induced dyslipidemia. Interestingly, prior physical training was able to partially protect against this rise in BP and body fat stores. Prior physical training did not totally protect against the effects of HFD consumption but previously trained animals did demonstrate resistance to the development of cardiometabolic alterations, which illustrate that the benefits of physical training may be partially maintained even after 30 days of detraining period.
Endocrine, Jan 20, 2018
The early-life nutritional environment affects long-term glucose homeostasis, we investigated the... more The early-life nutritional environment affects long-term glucose homeostasis, we investigated the effects of maternal low-protein diet combined with postnatal early overfeeding on the male offspring's glucose homeostasis in adulthood. Only male rats were used, and their delivery was considered postnatal-day 0 (PN0). Wistar rats' dams were divided into control (NP) or low-protein diet (LP). LP dams remained on the diet until PN14, after which all animals were supplied with the control diet. At PN2, litters were adjusted to 9 (control-NL) or 3 (postnatal-overfeeding-PO) pups, resulting in four experimental groups: NP-NL, NP-PO, LP-NL, and LP-PO. Litters were weaned on PN21. At PN80, a batch of animals from all experimental groups underwent surgery for cannula implantation, followed by intravenous glucose tolerance test (ivGTT), but the insulinogenic index (ISI) was calculated. At PN81, animals were euthanized and tissues were collected. LP-diet and early postnatal-overfeeding ...
Nutritional neuroscience, Jan 6, 2018
We aimed to assess the effects of a maternal protein-caloric restriction diet during late pregnan... more We aimed to assess the effects of a maternal protein-caloric restriction diet during late pregnancy on the metabolism of rat offspring fed a high-fat diet (HFD) during adulthood. During late pregnancy, rat dams received either a low-protein (4%; LP group) or normoprotein (23%; NP group) diet. After weaning, the offspring were fed a standard diet (Control; C). Male offspring (60 days old) from both groups were then fed either the C diet or HFD until they were 90 days old. The adult offspring and maternal metabolic parameters and autonomic nervous system (ANS) were then evaluated. Dams exhibited low body weight gain and food intake during the LP diet consumption. At lactation, these dams showed high body weight gain, hypoinsulinemia and hyperglycemia. The maternal LP diet resulted in low body weights for the pups. There were also no differences in the metabolic parameters between the adult LP offspring that were fed the C diet and the NP group. Adults of both groups that were fed the ...
International journal of endocrinology, 2018
Stevia rebaudiana (Bert.) Bertoni besides being a source of noncaloric sweeteners is also an impo... more Stevia rebaudiana (Bert.) Bertoni besides being a source of noncaloric sweeteners is also an important source of bioactive molecules. Many plant extracts, mostly obtained with ethyl acetate solvent, are rich in polyphenol compounds that present insulinotropic effects. To investigate whether the nonsweetener fraction, which is rich in phenolic compounds isolated from Stevia rebaudiana with the solvent ethyl acetate (EAF), has an insulinotropic effect, including interference at the terminals of the autonomic nervous system of the pancreatic islets of rats. Pancreatic islets were isolated from Wistar rats and incubated with EAF and inhibitory or stimulatory substances of insulin secretion, including cholinergic and adrenergic agonists and antagonists. EAF potentiates glucose-stimulated insulin secretion (GSIS) only in the presence of high glucose and calcium-dependent concentrations. EAF increased muscarinic insulinotropic effects in pancreatic islets, interfering with the muscarinic receptor subfamily M 3. Adrenergic inhibitory effects on GSIS were attenuated in the presence of EAF, which interfered with the adrenergic α 2 receptor. Results suggest that EAF isolated from stevia leaves is a potential therapy for treating type 2 diabetes mellitus by stimulating insulin secretion only in high glucose concentrations, enhancing parasympathetic signal transduction and inhibiting sympathetic signal transduction in beta cells.
Frontiers in physiology, 2018
Aerobic exercise training can improve insulin sensitivity in many tissues; however, the relations... more Aerobic exercise training can improve insulin sensitivity in many tissues; however, the relationship among exercise, insulin, and cancer cell growth is unclear. We tested the hypothesis that aerobic exercise training begun during adolescence can attenuate Walker 256 tumor growth in adult rats and alter insulin secretion. Thirty-day-old male Wistar rats engaged in treadmill running for 8 weeks, 3 days/week, 44 min/day, at 55-65% VO until they were 90 days old (TC, Trained Control). An equivalently aged group was kept inactive during the same period (SC, Sedentary Control). Then, half the animals of the SC and TC groups were reserved as the control condition and the other half were inoculated with Walker 256 cancer cells, yielding two additional groups (Sedentary Walker and Trained Walker). Zero mortalities were observed in tumor-bearing rats. Body weight (BW), food intake, plasma glucose, insulin levels, and peripheral insulin sensitivity were analyzed before and after tumor cell ino...
Scientific reports, Jan 9, 2017
Low intensity exercise during pregnancy and lactation may create a protective effect against the ... more Low intensity exercise during pregnancy and lactation may create a protective effect against the development of obesity in offspring exposed to overnutrition in early life. To test these hypotheses, pregnant rats were randomly assigned into 2 groups: Sedentary and Exercised, low intensity, on a rodent treadmill at 30% VO2Max /30-minute/session/3x/week throughout pregnancy and the lactation. Male offspring were raised in small litters (SL, 3 pups/dam) and normal litters (NL, 9 pups/dam) as models of early overnutrition and normal feed, respectively. Exercised mothers showed low mesenteric fat pad stores and fasting glucose and improved glucose-insulin tolerance, VO2max during lactation and sympathetic activity. Moreover, the breast milk contained elevated levels of insulin. In addition, SL of sedentary mothers presented metabolic dysfunction and glucose and insulin intolerance and were hyperglycemic and hyperinsulinemic in adulthood. SL of exercised mothers showed lower fat tissue ac...
International journal of endocrinology, 2016
Essential polyunsaturated fatty acids (PUFAs) prevent cardiometabolic diseases. We aimed to study... more Essential polyunsaturated fatty acids (PUFAs) prevent cardiometabolic diseases. We aimed to study whether a diet supplemented with a mixture of n-6/n-3 PUFAs, during perinatal life, attenuates outcomes of long-term metabolic dysfunction in prediabetic and obese mice. Seventy-day-old virgin female mice were mated. From the conception day, dams were fed a diet supplemented with sunflower oil and flaxseed powder (containing an n-6/n-3 PUFAs ratio of 1.2 : 1.0) throughout pregnancy and lactation, while control dams received a commercial diet. Newborn mice were treated with monosodium L-glutamate (MSG, 4 mg g(-1) body weight per day) for the first 5 days of age. A batch of weaned pups was sacrificed to quantify the brain and pancreas total lipids; another batch were fed a commercial diet until 90 days of age, where glucose homeostasis and glucose-induced insulin secretion (GIIS) as well as retroperitoneal fat and Lee index were assessed. MSG-treated mice developed obesity, glucose intole...
Toxicology, 2016
Acephate has been used extensively as an insecticide in agriculture. Its downstream sequelae are ... more Acephate has been used extensively as an insecticide in agriculture. Its downstream sequelae are associated with hyperglycemia, lipid metabolism dysfunction, DNA damage, and cancer, which are rapidly growing epidemics and which lead to increased morbidity and mortality rates and soaring health-care costs. Developing interventions will require a comprehensive understanding of which excess insecticides during perinatal life can cause insulin resistance and type 2 diabetes. A Wistar rat animal model suggests that acephate exposure during pregnancy and lactation causes alterations in maternal glucose metabolism and programs the offspring to be susceptible to type 2 diabetes at adulthood. Therapeutic approaches based on preventive actions to food contaminated with insecticides during pregnancy and lactation could prevent new cases of type 2 diabetes.
European Journal of Nutrition, 2016
collections. At 90 days of age, offspring were submitted to glucose tolerance test (ivGTT) and eu... more collections. At 90 days of age, offspring were submitted to glucose tolerance test (ivGTT) and euthanized for blood and tissue samples collection. Results MG mothers showed increase in glucose and fructosamine levels; however, they showed low insulin levels and failure in β-cell function (p < 0.05). MG mothers also showed dyslipidemia (p < 0.05). Moreover, breast milk had elevated levels of glucose, triglycerides, cholesterol and fructosamine and low insulin (p < 0.05). Interestingly, MG offspring had increased body weight and adipose tissue at adulthood, and they also showed glucose intolerance and failure in β-cell function (p < 0.05). Besides, MG offspring showed dyslipidemia (p < 0.05) increasing cardiovascular diseases risk. Conclusions Maternal MG treatment negatively affects the male rat offspring, leading to type 2 diabetes and dyslipidemia in later life, possibly by changes in breast milk composition.
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Papers by kesia Palma Rigo