Papers by joseph del rosario
Scientific Reports
Understanding brain function requires repeatable measurements of neural activity across multiple ... more Understanding brain function requires repeatable measurements of neural activity across multiple scales and multiple brain areas. In mice, large scale cortical neural activity evokes hemodynamic changes readily observable with intrinsic signal imaging (ISI). Pairing ISI with visual stimulation allows identification of primary visual cortex (V1) and higher visual areas (HVAs), typically through cranial windows that thin or remove the skull. These procedures can diminish long-term mechanical and physiological stability required for delicate electrophysiological measurements made weeks to months after imaging (e.g., in subjects undergoing behavioral training). Here, we optimized and directly validated an intact skull ISI system in mice. We first assessed how imaging quality and duration affect reliability of retinotopic maps in V1 and HVAs. We then verified ISI map retinotopy in V1 and HVAs with targeted, multi-site electrophysiology several weeks after imaging. Reliable ISI maps of V1...
SummaryRhythmic oscillations of neural activity permeate sensory systems. Studies in the visual s... more SummaryRhythmic oscillations of neural activity permeate sensory systems. Studies in the visual system propose that broadband gamma oscillations (30 – 80 Hz) facilitate neuronal communication underlying visual perception. However, broadband gamma oscillations within and across visual areas show widely varying frequency and phase, providing constraints for synchronizing spike timing. Here, we analyzed data from the Allen Brain Observatory and performed new experiments that show narrowband gamma (NBG) oscillations (50 – 70 Hz) propagate and synchronize throughout the awake mouse thalamocortical visual system. Lateral geniculate (LGN) neurons fired with millisecond precision relative to NBG phase in primary visual cortex (V1) and multiple higher visual areas (HVAs). NBG in HVAs depended upon retinotopically aligned V1 activity, and neurons that fired at NBG frequencies showed enhanced functional connectivity within and across visual areas. Remarkably, LGN ON versus OFF neurons showed d...
This data archive contains all code and data necessary to replicate each main text figure and ass... more This data archive contains all code and data necessary to replicate each main text figure and associated statistical comparisons in the following article: <br>Speed, A., Del Rosario, J., Mikail, N., & Haider, B. (2020). Spatial attention enhances network, cellular and subthreshold responses in mouse visual cortex. Nature communications, 11(1), 505. https://doi.org/10.1038/s41467-020-14355-4<br>The data and code is compatible with Matlab 2016b or later. <br>The data archive is organized as self-contained subfolders corresponding to the results found in each main figure (e.g., folder 'F1' for results of Figure 1). Each subfolder contains the minimally processed data structures and commented code for replication of the published figures. <br><br>Abstract of associated publication: Internal brain states strongly modulate sensory processing during behaviour. Studies of visual processing in primates show that attention to space selectively improves be...
Confronted today to increasing demand for innovation, engineers consider creativity as an indispe... more Confronted today to increasing demand for innovation, engineers consider creativity as an indispensable albeit weird technique. How to combine the engineering design requirements for robustness and value achievement with the creativity capacity to get unheard original and varied ideas? How to control a "creative design process" with the rigor praised by engineering design? Based on recent theory of design reasoning, C-K theory, this paper shows that value, robustness, variety and originality can be efficiently combined in a theoretically grounded, well-mastered process. It shows how to avoid two main creative design traps, pseudo-creativity and bounded creativity; and how to manage two different types of creative design, value achievement vs value exploration creativity. These processes are illustrated on one specific case, the design of a "smart shopping cart".
Sensory impairments are a core feature of autism spectrum disorder (ASD). These impairments affec... more Sensory impairments are a core feature of autism spectrum disorder (ASD). These impairments affect visual perception (Robertson and Baron-Cohen, 2017), and have been hypothesized to arise from imbalances in cortical excitatory and inhibitory activity (Rubenstein and Merzenich, 2003; Nelson and Valakh, 2015; Sohal and Rubenstein, 2019); however, there is little direct evidence testing this hypothesis in identified excitatory and inhibitory neurons during impairments of sensory perception. Several recent studies have examined cortical activity in transgenic mouse models of ASD (Goel et al., 2018; Antoine et al., 2019; Lazaro et al., 2019), but have provided conflicting evidence for excitatory versus inhibitory activity deficits. Here, we utilized a genetically relevant mouse model of ASD (CNTNAP2−/− knockout, KO; Arking et al., 2008; Penagarikano et al., 2011) and directly recorded putative excitatory and inhibitory population spiking in primary visual cortex (V1) while measuring visu...
Cell Reports, 2019
Many factors modulate the state of cortical activity, but the importance of cortical state variab... more Many factors modulate the state of cortical activity, but the importance of cortical state variability for sensory perception remains debated. We trained mice to detect spatially localized visual stimuli and simultaneously measured local field potentials and excitatory and inhibitory neuron populations across layers of primary visual cortex (V1). Cortical states with low spontaneous firing and correlations in excitatory neurons, and suppression of 3-to 7-Hz oscillations in layer 4, accurately predicted single-trial visual detection. Our results show that cortical states exert strong effects at the initial stage of cortical processing in V1 and can play a prominent role for visual spatial behavior in mice.
<br>This data archive contains all code and data necessary to replicate each main text and ... more <br>This data archive contains all code and data necessary to replicate each main text and supplementary figure and associated statistical comparisons in the following article:<br>Williams, B., Del Rosario, J., Muzzu, T., Peelman, K., Coletta, S., bichler, E. K., Speed, A., Meyer-Baese, L., Saleem, A. B. &amp; Haider, B. (2021). Spatial modulation of dark versus bright stimulus responses in the mouse visual system. Current Biology, 31, 1-8. <br>The data and code is compatible with Matlab 2018 or later.<br>The data archive is organized as self-contained subfolders corresponding to the results found in each main figure (e.g., folder 'F1' for results of Figure 1). Each subfolder contains code that loads the minimally processed data structures for replication of the published figures.<br>Abstract of associated publication: <br>A fundamental task of the visual system is to respond to both increases and decreases of luminance with action potentials (ON and OFF responses). OFF responses are stronger, faster, and more salient than ON responses in primary visual cortex (V1) of both cats and primates, but in ferrets and mice ON responses can be stronger, weaker, or balanced in comparison to OFF responses. These discrepancies could arise from differences in species, experimental techniques, or stimulus properties, particularly retinotopic location in the visual field, as has been speculated; however, the role of retinotopy for ON/OFF dominance has not been systematically tested across multiple scales of neural activity within species. Here, we measured OFF versus ON responses across large portions of visual space with silicon probe and whole-cell patch-clamp recordings in mouse V1 and lateral geniculate nucleus (LGN). We found that OFF responses dominated in the central visual field, whereas ON and OFF responses were more balanced in the periphery. These findings were consistent across local field potential (LFP), spikes, and subthreshold membrane potential in V1, and were aligned with spatial biases in ON and OFF responses [...]
International Journal of Public Health Science (IJPHS)
Filipino youths are the hope of Philippine society. However, cigarette smoking and binge drinking... more Filipino youths are the hope of Philippine society. However, cigarette smoking and binge drinking continually corrupt this aspiration. Mental health states interplay with the growing setback of substance consumption. The main objective of the study is to determine the efficacy of the multimodal intervention program among the participants in this study. Specifically, it answers the following objectives: i) Develop an intervention program to address the high anxiety, mild depression, and normal self-esteem of the participants and ii) Determine the significant difference between the control group and the experimental group in the pre-test and post-test intervention measures. A matched-group experimental design was carried out among the participants who were randomly assigned to the control group and the experimental group. There were forty participants who met the criteria set by the researchers. When the multimodal intervention program was tested using t-test to analyze the finding...
A fundamental task of the visual system is to respond to luminance increments and decrements. In ... more A fundamental task of the visual system is to respond to luminance increments and decrements. In primary visual cortex (V1) of cats and primates, luminance decrements elicit stronger, faster, and more salient neural activity (OFF responses) than luminance increments (ON responses). However, studies of V1 in ferrets and mice show that ON responses may be stronger. These discrepancies may arise from differences in species, experimental conditions, or from measuring responses in single neurons versus populations. Here, we examined OFF versus ON responses across different regions of visual space in both single neurons and populations of mouse V1. We used high-density silicon probes and whole-cell patch-clamp recordings to assess OFF versus ON dominance in local field potential (LFP), single neuron, and membrane potential responses. Across these levels, we found that OFF responses clearly dominated in the central visual field, whereas ON responses were more evident in the periphery. Thes...
Nature Communications
Internal brain states strongly modulate sensory processing during behaviour. Studies of visual pr... more Internal brain states strongly modulate sensory processing during behaviour. Studies of visual processing in primates show that attention to space selectively improves behavioural and neural responses to stimuli at the attended locations. Here we develop a visual spatial task for mice that elicits behavioural improvements consistent with the effects of spatial attention, and simultaneously measure network, cellular, and subthreshold activity in primary visual cortex. During trial-by-trial behavioural improvements, local field potential (LFP) responses to stimuli detected inside the receptive field (RF) strengthen. Moreover, detection inside the RF selectively enhances excitatory and inhibitory neuron responses to task-irrelevant stimuli and suppresses noise correlations and low frequency LFP fluctuations. Whole-cell patch-clamp recordings reveal that detection inside the RF increases synaptic activity that depolarizes membrane potential responses at the behaviorally relevant locatio...
SummaryMany factors modulate the state of cortical activity, but the importance of cortical state... more SummaryMany factors modulate the state of cortical activity, but the importance of cortical states for sensory perception remains debated. We trained mice to detect spatially localized visual stimuli, and simultaneously measured local field potentials and excitatory and inhibitory neuron populations across layers of primary visual cortex (V1). Cortical states with low firing rates and correlations between excitatory neurons, and reduced oscillatory activity in Layer 4, accurately predicted single trials of visual spatial detection behavior. Our results show that cortical states exert strong effects at the initial stage of cortical processing in V1, and play a decisive role for visual spatial behavior in mice.
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Papers by joseph del rosario