Protein kinase C (PKC) is a multigene family of at least ten isoforms, nine of which are expresse... more Protein kinase C (PKC) is a multigene family of at least ten isoforms, nine of which are expressed in brain (␣, I, II, ␥, ␦, ⑀, , , /). Our previous studies have shown that many of these PKCs participate in synaptic plasticity in the CA1 region of the hippocampus. Multiple isoforms are transiently activated in the induction phase of long-term potentiation (LTP). In contrast, a single species, , is persistently activated during the maintenance phase of LTP through the formation of an independent, constitutively active catalytic domain, protein kinase M (PKM). In this study, we used immunoblot and immunocytochemical techniques with isoform-specific antisera to examine the distribution of the complete family of PKC isozymes and PKM in rat brain. Each form of PKC showed a widespread distribution in the brain with a distinct regional pattern of high and low levels of expression. PKM, the predominant form of PKM in brain, had high levels in hippocampus, frontal and occipital cortex, striatum, and hypothalamus. In the hippocampus, each isoform was expressed in a characteristic pattern, with prominent in the CA1 stratum radiatum. These results suggest that the compartmentalization of PKC isoforms in neurons may contribute to their function, with the location of PKM prominent in areas notable for long-term synaptic plasticity.
Protein kinase C (PKC) is a multigene family of at least ten isoforms, nine of which are expresse... more Protein kinase C (PKC) is a multigene family of at least ten isoforms, nine of which are expressed in brain (␣, I, II, ␥, ␦, ⑀, , , /). Our previous studies have shown that many of these PKCs participate in synaptic plasticity in the CA1 region of the hippocampus. Multiple isoforms are transiently activated in the induction phase of long-term potentiation (LTP). In contrast, a single species, , is persistently activated during the maintenance phase of LTP through the formation of an independent, constitutively active catalytic domain, protein kinase M (PKM). In this study, we used immunoblot and immunocytochemical techniques with isoform-specific antisera to examine the distribution of the complete family of PKC isozymes and PKM in rat brain. Each form of PKC showed a widespread distribution in the brain with a distinct regional pattern of high and low levels of expression. PKM, the predominant form of PKM in brain, had high levels in hippocampus, frontal and occipital cortex, striatum, and hypothalamus. In the hippocampus, each isoform was expressed in a characteristic pattern, with prominent in the CA1 stratum radiatum. These results suggest that the compartmentalization of PKC isoforms in neurons may contribute to their function, with the location of PKM prominent in areas notable for long-term synaptic plasticity.
Background/Aims: Our group has been involved in searching for different strategies to ameliorate ... more Background/Aims: Our group has been involved in searching for different strategies to ameliorate hepatic cirrhosis. The aim of this study was to evaluate the effect of Pirfenidone in the reversion or prevention of cirrhosis experimentally induced in rats by chronic administration of CCl 4 and bile-duct ligation (BDL).
Protein kinase C (PKC) is a multigene family of at least ten isoforms, nine of which are expresse... more Protein kinase C (PKC) is a multigene family of at least ten isoforms, nine of which are expressed in brain (␣, I, II, ␥, ␦, ⑀, , , /). Our previous studies have shown that many of these PKCs participate in synaptic plasticity in the CA1 region of the hippocampus. Multiple isoforms are transiently activated in the induction phase of long-term potentiation (LTP). In contrast, a single species, , is persistently activated during the maintenance phase of LTP through the formation of an independent, constitutively active catalytic domain, protein kinase M (PKM). In this study, we used immunoblot and immunocytochemical techniques with isoform-specific antisera to examine the distribution of the complete family of PKC isozymes and PKM in rat brain. Each form of PKC showed a widespread distribution in the brain with a distinct regional pattern of high and low levels of expression. PKM, the predominant form of PKM in brain, had high levels in hippocampus, frontal and occipital cortex, striatum, and hypothalamus. In the hippocampus, each isoform was expressed in a characteristic pattern, with prominent in the CA1 stratum radiatum. These results suggest that the compartmentalization of PKC isoforms in neurons may contribute to their function, with the location of PKM prominent in areas notable for long-term synaptic plasticity.
Protein kinase C (PKC) is a multigene family of at least ten isoforms, nine of which are expresse... more Protein kinase C (PKC) is a multigene family of at least ten isoforms, nine of which are expressed in brain (␣, I, II, ␥, ␦, ⑀, , , /). Our previous studies have shown that many of these PKCs participate in synaptic plasticity in the CA1 region of the hippocampus. Multiple isoforms are transiently activated in the induction phase of long-term potentiation (LTP). In contrast, a single species, , is persistently activated during the maintenance phase of LTP through the formation of an independent, constitutively active catalytic domain, protein kinase M (PKM). In this study, we used immunoblot and immunocytochemical techniques with isoform-specific antisera to examine the distribution of the complete family of PKC isozymes and PKM in rat brain. Each form of PKC showed a widespread distribution in the brain with a distinct regional pattern of high and low levels of expression. PKM, the predominant form of PKM in brain, had high levels in hippocampus, frontal and occipital cortex, striatum, and hypothalamus. In the hippocampus, each isoform was expressed in a characteristic pattern, with prominent in the CA1 stratum radiatum. These results suggest that the compartmentalization of PKC isoforms in neurons may contribute to their function, with the location of PKM prominent in areas notable for long-term synaptic plasticity.
Background/Aims: Our group has been involved in searching for different strategies to ameliorate ... more Background/Aims: Our group has been involved in searching for different strategies to ameliorate hepatic cirrhosis. The aim of this study was to evaluate the effect of Pirfenidone in the reversion or prevention of cirrhosis experimentally induced in rats by chronic administration of CCl 4 and bile-duct ligation (BDL).
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Papers by ivan hernandez