Papers by kenneth fernandes
Habitat International, 2002
PII: S 0 1 9 7 -3 9 7 5 ( 0 1 ) 0 0 0 1 7 -0 Urban development and new towns in the Third World, ... more PII: S 0 1 9 7 -3 9 7 5 ( 0 1 ) 0 0 0 1 7 -0 Urban development and new towns in the Third World, lessons from the new Bombay experience Alain R. A. Jacquemin (Ed.); Ashgate, Aldershot, Hants, 1999, xvii+323pp., UK Pounds 42.50
Habitat International, 2002
PII: S 0 1 9 7 -3 9 7 5 ( 0 1 ) 0 0 0 1 7 -0 Urban development and new towns in the Third World, ... more PII: S 0 1 9 7 -3 9 7 5 ( 0 1 ) 0 0 0 1 7 -0 Urban development and new towns in the Third World, lessons from the new Bombay experience Alain R. A. Jacquemin (Ed.); Ashgate, Aldershot, Hants, 1999, xvii+323pp., UK Pounds 42.50
Activation of apoptosis is believed to be critical for the role of p53 as a tumor suppressor. Her... more Activation of apoptosis is believed to be critical for the role of p53 as a tumor suppressor. Here, we report a new mouse strain carrying a human p53 transgene in the mouse p53-null background. Expression of human p53 in these mice was comparable with wild-type murine p53; however, transactivation, induction of apoptosis, and G 1 -S checkpoint, but not transrepression or regulation of a centrosomal checkpoint, were deregulated. Although multiple functions of p53 were abrogated, mice carrying the human p53 transgene did not show early onset of tumors as typically seen for p53-null mice. In contrast, human p53 in the p53-null background did not prevent accelerated tumor development after genotoxic or oncogenic stress. Such behavior of human p53 expressed at physiologic levels in transgenic cells could be explained by unexpectedly high binding with Mdm2. By using Nutlin-3a, an inhibitor of the interaction between Mdm2 and p53, we were able to partially reconstitute p53 transactivation and apoptosis in transgenic cells. Our findings indicate that the interaction between p53 and Mdm2 controls p53 transcriptional activity in homeostatic tissues and regulates DNA damage-and oncogene-induced, but not spontaneous, tumorigenesis. (Cancer Res 2006; 66(6): 2928-36) Note: A.J. Fornace, Jr., is currently at the
Breast Cancer Research, 2008
Febs Letters, 2010
a b s t r a c t p53 gene expresses several protein isoforms modulating p53-mediated responses thr... more a b s t r a c t p53 gene expresses several protein isoforms modulating p53-mediated responses through regulation of gene expression. Here, we identify a novel p53 isoform, D160p53, lacking the first 159 residues. By knockdown experiments and site-directed mutagenesis, we show that D160p53 is encoded by D133p53 transcript using ATG160 as translational initiation site. This hypothesis is supported by endogenous expression of D160p53 in U2OS, T47D and K562 cells, the latter ones carrying a premature stop codon that impairs p53 and D133p53 protein expression but not the one of D160p53. Overall, these results show that the D133p53 transcript generates two different p53 isoforms, D133p53 and D160p53.
Breast Cancer Research, 2010
not available at time of publication.
Cell Death and Differentiation, 2011
These authors contributed equally to this work.
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Papers by kenneth fernandes