Papers by fereshte ghandehari
Research Square (Research Square), Feb 28, 2024
Introduction: Microcins are Antimicrobial peptides (AMPs) with low molecular weight, which are pr... more Introduction: Microcins are Antimicrobial peptides (AMPs) with low molecular weight, which are produced by Enterobacterales and have broad-spectrum antibacterial activity. They can selectively replace common cancer treatments in cancer cells with less side effects and higher effectiveness. Given the aforementioned context, the present study endeavors to examine the antitumor activity of microcins isolated from of the Enterobacterales. Material and Methods In total, 120 Enterobacterales isolates were examined after identi cation. Subsequently, the bacteria were subjected to an agar diffusion test to assess their antibacterial e cacy. Positive isolates were further examined for the presence of Mccj25 using PCR. The cytotoxic effects of isolates harboring the microcin gene were explored using quantitative real-time PCR (RT-qPCR) and the MTT test on breast cancer cells. Additionally, the expression levels of BCL2 and STAT3 genes were evaluated, and apoptosis was quanti ed using ow cytometry. The repair rate of normal cells was determined using a scratch assay. Results The ndings obtained from the phenotypic and biochemical assays have duly veri ed and established the categorization of the Enterobacterales. After conducting the agar diffusion test, a total of 25 isolates of Escherichia coli and Klebsiella pneumoniae displaying inhibition zones were chosen as suitable specimens possessing AMPs. Urinary E. coli was identi ed as isolate 83. The analysis conducted on the expression of the Mccj25 gene within the aforementioned isolates indicated that isolate 83 exhibited signi cant expression of the Mccj25 gene. Conclusion The extract obtained from this isolate on the breast cancer cell line exhibited the most signi cant degree of toxicity after precisely 48 h. Furthermore, the treatment of breast cancer cells with isolate 83 showed that the rate of apoptosis was about 86%, and the expression of BCL2 and STAT3 genes decreased. Moreover, it potentiated the reparative ability of normal broblast cells. They resulted in growth suppression of breast cancer cells and elicited an escalated rate of cellular demise via the apoptosis pathway. the use of medicinal drugs with fewer side effects has received considerable attention in recent years. Antimicrobial peptides (AMPs) are a class of anti-cancer peptides. AMPs have a wide range of antibacterial, antiviral, antifungal, and anticancer properties, and are part of the intrinsic resistance to microorganisms [3]. AMPs, members of the class of anticancer peptides, are among these substances. AMPs have a wide range of antibacterial, antiviral, antifungal, and anticancer properties and are part of the intrinsic resistance reaction to microorganisms [3]. Numerous studies have reported the use of AMPs against cancer cells, including breast, lung, lymphoma, leukemia, and myeloma cells [4].. E. coli bacteriocins are divided into colicins (25-80 kDa) and microcins (1-10 kDa) based on their molecular weight [5, 6]. Microcins possess an advantage over colicins as they do not pose lethality to the strains that produce them. [7]. There are two types of microcins. Class I microcins have a molecular mass of less than 5 kDa and are highly post-translationally modi ed, which include microcin B17 (MccB17), MccJ25, MccC7/C51, and MccD93. In contrast, class II peptide microcins are larger (5-10 kDa) and are further divided into two subclasses, IIa and IIb. Accordingly, subclass IIa does not require post-translational modi cations, but can contain disul de bonds, including the plasmid-mediated microcins MccL, MccV, and MccS, and siderophore bacteriocins, known as class IIb microcins, including MccE492, MccM, MccI47, and MccH47 [8]. The signi cance of utilizing microcins as possible alternatives to antibiotics is attributable to the absence of bacterial resistance to these AMPs [9]. In addition to their antimicrobial properties, microcins have antitumor functions and can act through various mechanisms such as apoptosis, necrosis, and pore creation in the target cell membrane, thereby inhibiting angiogenesis and activating the immune system against cancer cells [10]. Recently, researchers have exhibited signi cant interest in the potential use of microcins for cancer treatment because of their selective nature, which results in higher e cacy and minimal adverse effects. [9, 10]. MccJ25 is a 21-amino acid peptide produced by some Enterobacterales, especially E. coli, and has a stable structure to avoid degradation by proteases in the digestive tract [8]. MccJ25 has antimicrobial activity and as a membrane-active peptide, it causes the death of eukaryotic cells by destroying cytochrome c [11, 12]. Against this background, in the current study, we examined how breast cancer cells respond to isolated MccJ25 from E. coli. 2. Materials and Methods 2.1. isolate collection, culture, and identi cation E. coli were isolated and con rmed from urine samples obtained from private laboratories. In brief, 120 Enterobacterales isolates were cultured on blood agar and eosin methylene blue at 35°C for 18-24 h. Next, E. coli isolates were identi ed and isolated using different phenotypic characteristics and biochemical tests, including Voges Proskauer (VP)
Jundishapur Journal of Microbiology, Nov 29, 2023
Background: In hospitals and communities, Methicillin-resistant Staphylococcus aureus (MRSA) play... more Background: In hospitals and communities, Methicillin-resistant Staphylococcus aureus (MRSA) plays a critical role due to its ability to acquire resistance against several antibiotics and play a role in the spread of diseases. Objectives: This research aimed to investigate the pattern of antibiotic resistance in MRSA isolates and perform molecular typing of MRSA isolates using various elements, including SCCmec type, ccr type, prophage type, and gene toxin profiles. Methods: The research spanned 20 months at Al-Zahra Hospital in Isfahan and involved 148 isolates from various anatomical sites. The isolates were evaluated for their antibiotic susceptibility patterns. They were characterized by screening for SCCmec typing, ccr typing, phage typing, and PCR profiling of pvl, hlb, sak, eta, and tst toxin genes. Results: From 148 total S. aureus isolates, 42% (n = 62) were methicillin-resistant. The MRSA isolates demonstrated substantial resistance to penicillin and ciprofloxacin, and 90.3% of MRSA isolates were multiple-drug resistant. Also, SCCmec types III, I, and IV were identified in 45.16%, 35.48%, and 19.35% of MRSA isolates, respectively. Also, seven prophage patterns and 15 toxin patterns were detected among MRSA isolates. Conclusions: Multi-drug resistance is common among MRSA isolates. The only effective drug among the investigated antibiotics was chloramphenicol. The MRSA isolates can be controlled by changing the prescribing procedure of antibiotics and applying infection control strategies. The studied MRSA isolates can cause a wide range of diseases due to having several bacteriophages that encode virulence factors. Identification of different types of prophages may be useful in predicting such pathogenic agents.
Asian journal of green chemistry, Jul 1, 2018
Nowadays, tend to use nanotechnology in various fields such as medical science and pharmacology h... more Nowadays, tend to use nanotechnology in various fields such as medical science and pharmacology have increased. Making nanoparticles can be done by different ways, but, due to the hazards and environmental pollution caused by them, green synthesis has attracted much attention. Green synthesis of biological resources such as plants, green algae, and microorganisms like bacteria and yeast are used for the production of nanoparticles. For the production of iron oxide nanoparticles this research, in line with the objectives of green synthesis, used the lactobacillus casei extract as a biological source. In this study, green synthesis of iron oxide nanoparticles were performed using lactobacillus casei extract as a biological source cytoplasmic extract of lactobacillus casei and iron sulfate solution 10-3 M [pH=5.6] were mixed in a V/V 10% volume ratio, and incubated for 3 weeks at 37 °C in the presence of 5% carbon dioxide. Synthesizing iron oxide nanoparticles was studied by electron microscope and x-ray microdiffraction. After three weeks of incubation, the color of iron sulfate and the extract solution was changed from colorless to black. According to XRD analysis, synthesis of iron oxide nano crystals was confirmed. The average synthesized nanoparticles diameters as determined by transmission electron microscopy (TEM) was found to be about 15 nm with a spherical shape. Production of iron oxide nanoparticles through green synthesis method using cytoplasmic extract of lactobacillus casei as a microorganism probiotic is biologically safe, of low cost, simple, efficient, and eco-friendly treatment that has attracted a lot of attention in medicine, pharmacology, and targeted drug delivery.
Iranian Journal of Toxicology
Background: Kidneys are the most vulnerable organs with respect to oxidative stress caused by lea... more Background: Kidneys are the most vulnerable organs with respect to oxidative stress caused by lead poisoning. In this study, renal biomarkers were investigated in rats, exposed to lead after treatment with or without Lactobacillus fermentum. Methods: Twenty-four rats were divided into four groups of five each as follows: a) control, b) lead-exposed, c) Lactobacillus fermentum–treated, and d) rats exposed to lead followed by treatment with L. fermentum. After eight weeks of treatment, the renal biomarkers in blood and antioxidant factors in the kidneys were evaluated. The kidneys were also examined histopathologically for alterations due to lead exposure. Results: In lead-exposed rats, the creatinine, urea, uric acid, malondialdehyde, and tissue lead contents were significantly higher, while catalase, glutathione peroxidase and glutathione levels were lower than those found in the controls. After treatment of lead-exposed rats with L. fermentum, the levels of these factors were signi...
Journal of Kerman University of Medical Sciences
Background: Osteomyelitis is a bone infection. The most common treatment is use of antibiotics, w... more Background: Osteomyelitis is a bone infection. The most common treatment is use of antibiotics, which might have many side effects and may induce antibiotic resistance in bacteria. Strengthening the body›s antioxidant system may be effective on preventing the disease progression. The aim of this study was to investigate the effects of Lactobacillus plantarum and L. plantarum enriched with selenium (Se) on osteomyelitis caused by Staphylococcus aureus. Methods: Thirty-six male rats were randomly divided into the 6 groups. A group was control group. Groups B and C were respectively treated with L. plantarum, and L. plantarum enriched with Se. Osteomyelitis was induced in group D. Groups E and F were induced with osteomyelitis and treated like groups B and C. At the end of the treatment period, in order to evaluate hematological parameters, the acute phase protein, interleukin 6 and 1- levels were assessed in the rat’s blood samples. Bone was stained with hematoxylin-eosin to evaluate ...
Journal of Biomolecular Structure & Dynamics, Nov 15, 2022
Pakistan Journal of Pharmaceutical Sciences, Sep 1, 2021
The resistance of Acinetobacter baumannii to most antibiotics is increasing. The presence of meta... more The resistance of Acinetobacter baumannii to most antibiotics is increasing. The presence of metallo-beta-lactamase and carbapenemase enzymes has led to the resistance of these bacteria to carbapenems as one of the major classes of broad-spectrum antibiotics and has raised concerns in human societies. This research evaluated the presence of blaOXA-51, blaOXA-58 and blaOXA-23 genes in A. baumannii strains during a 12 months period. One hundred strains were isolated from the patients hospitalized in ICU of Ali Asghar and Shahid Rajaee trauma hospitals in Shiraz. Bacterial identity was determined by biochemical tests and antibiotic resistance was determined by disk diffusion method. The isolated strains were then evaluated in terms of carrying blaOXA-23, blaOXA-51 and blaOXA-58 genes, using the multiplex PCR method. The results showed that A. baumannii was resistant to carbapenems but most strains were susceptible to tigecyclin and colistin. The majority of strains carried the blaOXA-23 and blaOXA-51 genes, but very few carried the bla OXA-58 gene. The results revealed that the antibiotic resistance of A. baumannii is increasing, which causes a more outbreak of this organism.
Journal of Cell & Tissue, 2021
Aim: This research aimed to study the anticancer effects of nano-selenium oxide-enriched Saccharo... more Aim: This research aimed to study the anticancer effects of nano-selenium oxide-enriched Saccharomyces bullardi on DMBA-induced breast cancer cells in rats. Material and Methods: In order to induce cancer, the carcinogen with a concentration of 60 mg/kg was injected into the left breast nipple of the female rats. When the tumors grew to about 10 mm, the cancerous tumor was isolated from the breast of the animals and used to make tissue sections and individual cells. Cancer cells were treated with different concentrations of S. boulardi suspension and nano-selenium oxide-enriched S. boulardi. The cytotoxicity assay was performed with two methods of MTT and Trypan blue staining. Results: Comparison between two cell groups treated with S. boulardii and nano-selenium oxideenriched S. boulardi using two tests, it was found that there were significant changes in cancer cells viability in both 0.5,1 µg/ml concentrations. with increasing the concentration, the cancer cell viability was significantly decreased. cell viability in the cell line treated with nano-selenium oxide-enriched S. boulardi yeast, with the increase in the concentration of enriched yeast was decreased. A comparison between the yeast and enriched yeast in different concentrations, revealed that there was a significant difference between cytotoxicity effects of them on the cells. Conclusion: Probiotic yeasts could be an appropriate candidate for the treatment of some diseases including cancers, especially when combined with selenium compounds.
Journal of Kermanshah University of Medical Sciences, 2018
Objectives: Antimicrobial peptides belong to a large family of bioactive peptides that play an im... more Objectives: Antimicrobial peptides belong to a large family of bioactive peptides that play an important role in human health. Lactoferrin of transferrin family is considered to be a glycoprotein. Human lactoferrin has a length of 689-711 amino acid polypeptides. Lactoferrin plays a role in various biological activities such as anticancer, antibacterial, antiviral, antifungal, and antiinflammatory activity, and also as an immune system regulator. In this study, we examined the toxic effects of human lactoferrinderived peptides on breast cancer cells in vitro using the web server Anticp. The results obtained from the Anticp web server showed that four peptides (P39, P40, P24, P66), out of the 70 peptides, had anticancer activity, and 66 peptides had non-anticancer activity. Methods: The cytotoxicity of two peptides with high cytotoxic activity (P39, P40) and a peptide with low cytotoxic activity (P38) on breast cancer cells (MCF-7, MDA-MB-231) was examined using the MTT assay. The CC50 values of the peptides (P39, P40) were 100 µg/mL for MCF-7 cells and 950 and 1000 µg/mL for MDA-MB-231 cells, respectively. Results: The results showed that the activity of the cytotoxic peptides P39 and P40 was significantly higher than that of P38 on the breast cancer cells (P ≤ 0.001). Cancer cells treated with CC50 concentrations showed a percentage of the cells detached from the flask and surrounding cells as well as shrinkage and deformation on the surface. Also, apoptotic seeds inside the cells were observed with 40X magnification. Discussion: These finding demonstrated that P39 and P40 peptides could be appropriate candidates for in vivo testing as cytotoxic agents.
New Cellular and Molecular Biotechnology Journal, 2019
Aim and Background: In recent years, science and industry have focused on preparing nanoparticles... more Aim and Background: In recent years, science and industry have focused on preparing nanoparticles based on the principles of green chemistry. For this purpose, various types of biological structures such as bacteria, yeasts, and string molds are used. Due to the resistance of bacteria to antibiotics, the need to replace effective antimicrobials, with fewer side effects is less. The aim of this research was to synthesis the iron oxide nanoparticles by lactobacillus fermentum cytoplasmic extract and investigate its antimicrobial effects against Staphylococcus aureus and Pseudomonas aeruginosa. Materials and Methods: In this study, after preparing cytoplasmic extract of Lactobacillus fermentum from frees-thow method, iron sulfate solution 10 M were added and incubated for 3 weeks in the presence of 5 % carbon dioxide. Production of nanoparticles was investigated by X-ray Diffraction (XRD) and Transmission Electron Microscopy (TEM) and finally, the antimicrobial effects of standard stra...
Journal of Shahrekord University of Medical Sciences, 2020
Background and aims: Antimicrobial peptides constitute a family of bioactive peptides that are in... more Background and aims: Antimicrobial peptides constitute a family of bioactive peptides that are involved in the body defense. Recently, their anti-cancer properties, especially by inducing apoptosis, have been proven in in vitro studies. Therefore, in this study, the effects of cecropin B as an antimicrobial peptide on breast cancer growth, hematological parameters, and histopathological changes in rats were evaluated. Methods: Twenty-four female rats were randomly divided into 4 groups. The cancer group, control group, cecropin B group, and cancer group treated with cecropin B. The tumor size was measured at the beginning and the completion of the treatment period. Blood samples were collected for assessment of the hematological parameters and Bax and Bcl2 levels. Tumor tissues were removed for histopathological analysis. Results: The tumor size had a significant increase in the cancer group and cancer group treated with cecropin at the end of the treatment. A significant decrease i...
Journal of Medicinal and Chemical Sciences, 2018
In the past decade, the attention of science and technology has focused on production of nanopart... more In the past decade, the attention of science and technology has focused on production of nanoparticles. There are various ways for synthesizing of nanoparticles that many of them are not cost effective due to power and material consumption. Therefore, production of nanoparticles through biologic ways is needed. For this purpose, different biological structures such as plants, algae, and microorganisms such as bacteria, string molds, and yeasts are used for nanoparticles production. This study is focused on biosynthesis of iron oxide nanoparticles by cytoplasmic extracts of bacteria Lactobacillus Fermentum, which is a probiotic microorganism, based on the method of green chemistry. After preparation of cytoplasmic extract of bacteria Lactobacillus Fermentum through freez-thow method, iron sulfate solution (III) with a concentration of 10-3 M was added in an equal volume ratio (V / V% 10) and incubated for 3 weeks at 37 ° C in the presence of 5% carbon dioxide. Production of nanoparti...
International Journal of Academic Research in Accounting, Finance and Management Sciences, 2013
The purpose of this study is to investigate the role of personality traits in perfectionism orien... more The purpose of this study is to investigate the role of personality traits in perfectionism orientation among Isfahan Melli Bank employees. The statistical population of this study consists of the employees of Melli Bank in the city of Isfahan in the third season of 2012. The sample size was 150 and then the respondents were selected by random sampling method. Data collection instrument was self-administrated questionnaire that included 56 items in order to measure the variables. So as to examine the validity of the questionnaire, content validity was used and afterwards, the questionnaire was corrected and modified by some faculty members. After conducting a primary sampling, the final version of the questionnaire was developed. Also Cronbach’s Alpha was used to examine reliability of the questionnaire whose coefficient was 87% which is an acceptable value to confirm the reliability. In order to analyze the data and conclude the results, descriptive and inferential statistics were ...
Nowadays, tend to use nanotechnology in various fields such as medical science and pharmacology h... more Nowadays, tend to use nanotechnology in various fields such as medical science and pharmacology have increased. Making nanoparticles can be done by different ways, but, due to the hazards and environmental pollution caused by them, green synthesis has attracted much attention. Green synthesis of biological resources such as plants, green algae, and microorganisms like bacteria and yeast are used for the production of nanoparticles. For the production of iron oxide nanoparticles this research, in line with the objectives of green synthesis, used the lactobacillus casei extract as a biological source. In this study, green synthesis of iron oxide nanoparticles were performed usingLactobacillus casei extract as a biological source cytoplasmic extract of lactobacillus casei and iron sulfate solution 10-3 M [pH=5.6] were mixed in a V/V 10 % volume ratio, and incubated for 3 weeks at 37 °C in the presence of 5% carbon dioxide. Synthesizing iron oxide nanoparticles was studied by electron...
Avicenna Journal of Phytomedicine, 2018
Objective: In traditional medicine, Ficus carica (also known as fig) latex is recognized as a rem... more Objective: In traditional medicine, Ficus carica (also known as fig) latex is recognized as a remedy with various therapeutic effects. Recently, in vitro studies have reported the anticancer effect of this latex on cancer cell lines. This study evaluated the effect of this latex on breast cancer growth, hematological parameters, and histopathology in rats. Materials and Methods: Twenty-four female rats were randomly divided into 3 groups. In cancerous group, 0.5 ml 7, 12-dimethylbenz (a) anthracene was injected to nipple for breast cancer induction. The control group received sesame oil at the same volume through similar route. In fig latex treated group (Fle), breast cancer was induced and then 0.5 ml of fig latex was intratumorally injected on a daily basis for 4 weeks. Tumor size was measured at the 2nd, 4th and 6th weeks of the experiment. Blood samples were used for investigation of the hematological parameters and livers, kidneys and tumor tissues were removed for histopatholo...
Background: The most important strategy of the immune system against pathogens is producing activ... more Background: The most important strategy of the immune system against pathogens is producing active oxygen intermediates with sidelong consequence of oxidative stress induction in body. Probiotics and selenium have recently been proven to be powerful antioxidants that help boost the immune system. Hence, the aim of this study was to investigate the antioxidant effects of Saccharomyces boulardii and selenium-enriched S. boulardii against oxidative stress induction caused by S. aureus in rats. Methods: A total of 36 rats were divided into 6 groups. A: control group, B: S. boulardii treated group, C: selenium-enriched S. boulardii treated group, D: S. aureus infected group, E: S. aureus infected and S. boulardii treated group and F: S. aureus infected and selenium-enriched S. boulardii treated group. At the end of the treatment period, animals were anesthetized and blood samples were collected to measure blood cell count, their indexes and antioxidant factors. Results: There was no sign...
Zahedan Journal of Research in Medical Sciences, 2021
Background: The reaction of immune cells to the introduction of pathogens into the body is an inc... more Background: The reaction of immune cells to the introduction of pathogens into the body is an increase in reactive oxygen species, which leads to the induction of oxidative stress. Reducing the level of oxidative stress through the use of antioxidants strengthens the immune system. Objectives: This study aimed to investigate the antioxidant effects of Saccharomyces cerevisiae and selenium-enriched S. cerevisiae against the oxidative stress induced by Pseudomonas aeruginosa in rats. Methods: A total of 36 rats were randomly divided into six groups, including control (A), S. cerevisiae (B), S. cerevisiae enriched with selenium (C), P. aeruginosa infection treated with S. cerevisiae (D), P. aeruginosa infection treated with S. cerevisiae enriched with selenium (E), and P. aeruginosa infection (F). At the end of the treatment period, the blood samples were collected to evaluate hematological parameters, total antioxidant capacity, glutathione concentration, and glutathione peroxidase ac...
European Cytokine Network, 2020
BACKGROUND Hepatitis C virus (HCV) is the leading cause of chronic liver diseases including hepat... more BACKGROUND Hepatitis C virus (HCV) is the leading cause of chronic liver diseases including hepatic fibrosis, cirrhosis, and hepatocellular carcinoma. We aimed to assess serum levels of interleukin (IL)-22, IL-27 and IL-35 in patients with hepatitis C and healthy controls to investigate their possible relationship with viral genotypes and liver enzyme levels. METHOD A total of 30 newly diagnosed hepatitis C patients with no history of antiviral therapy and 30 healthy individuals participated in this study. Serum levels of IL-22, IL-27 and IL-35 were determined by ELISA in peripheral blood samples from patients prior to and following treament with pan-genotypic direct-acting anti-viral therapy. Serum levels of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) were measured to determine any possible association between hepatic enzymes and cytokine serum levels concentrations. RESULT The results show elevated serum levels of of IL-35 in HCV-infected patients compared to treated cases and healthy controls, whereas there was no significant difference in IL-22 and IL-27 serum levels among the three groups. Additionally, the cytokine levels were not significantly correlated with certain genotypes and levels of liver enzymes. CONCLUSION Our findings indicate a potential role for IL-35 in chronic HCV infection and therapeutic management of patients with hepatitis C infection.
Meta Gene, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
journal of ilam university of medical sciences, 2019
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Papers by fereshte ghandehari