Buletin Fakultas Peternakan Universitas Gadjah Mada/Buletin Peternakan, May 31, 2024
Phospholipase in buck seminal plasma will trigger a coagulation incident resulting in the demise ... more Phospholipase in buck seminal plasma will trigger a coagulation incident resulting in the demise of the spermatozoa during liquid semen processing using milk or egg yolk substrates diluent. A plasma replacement process is performed to avoid this possibility. The cryopreservation process leads to sperm cell damage due to the freezing process, therefore cryoprotectant agents such as saccharides are required as protective agents. This study aimed to investigate the effect of various types of saccharides on the quality of frozen semen of etawah crossbreed (PE) bucks during cryopreservation and thawing with plasma replacement using Priangan ram semen plasma. Semen was collected using an artificial vagina once a week. Fresh PE bucks semen centrifuged at 3,000 RPM for 30 min. The supernatant (seminal plasma) was removed and replaced in equal volume with Priangan ram seminal plasma. Semen was divided into four treatments: goat semen with sheep semen plasma in Tris diluent (control); control with 0.6% dextrose added (monosaccharides); control with 0.6% sucrose added (disaccharides); control with 0.6% raffinose added (trisaccharides). Semen quality including percentages of motile spermatozoa, live spermatozoa, and intact plasma membrane (IPM) were evaluated after diluted, equilibrated, and thawing, respectively. Results of this study showed that viability and IPM of monosaccharides, disaccharides, and trisaccharides were significantly (P<0.05) higher than control (54.40 and 51.40; 55.00 and 53.60; 55.60 and 52.20 vs 48.40 and 52.20, respectively). The motility of disaccharides was significantly (P<0.05) higher compared to control and other diluent (47.00 vs 41.00, respectively). In conclusion, different types of saccharides were effective in maintaining the quality of etawah crossbreed buck frozen semen. The addition of disaccharides seems more effective compared to monosaccharides and trisaccharides in tris extender on the quality of etawah crossbreed buck frozen semen.
Advances in Animal and Veterinary Sciences, Dec 31, 2022
The objective of this study was to compare the effects of enzymatic (EZ) and non-enzymatic (NEZ) ... more The objective of this study was to compare the effects of enzymatic (EZ) and non-enzymatic (NEZ) antioxidants on the quality of spermatozoa in chilled, stored liquid semen from Garut rams. The rams were randomly divided into seven treatments in a 2 × 3 factorial design. The two factors were the antioxidant levels of EZ (25, 50, and 75 mg) and NEZ (5, 10, and 20 mg). Motility, viability, and plasma membrane integrity were the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4 th day of observation showed that the addition of ascorbic acid as NEZ at a dose of 20 mg/100 ml into the egg yolk tris extender resulted in the greatest preservation of ram sperm quality (P<0.05), such as motility and viability. Hence, the addition of catalase as EZ at a dose of 50 mg/100 ml to the egg yolk tris extender resulted in the highest (P<0.05) percentage of motility, percentage of live spermatozoa, and intact plasma membrane. The NEZ group showed better results (P<0.05) than the NEZ group in maintaining motility, viability, and membrane integrity. The research concluded that the administration of NEZ at a dose of 20 mg/100 ml extender and EZ treatment at 50 mg/100 ml extender represents the optimal dose to preserve sperm quality until day four of dilution. In addition, the NEZ antioxidant showed better-improved quality of spermatozoa in the liquid semen of Garut rams compared to the EZ antioxidant.
Pathogenecity of Escherichia coli could be known by the determination of infective dose-50 in in ... more Pathogenecity of Escherichia coli could be known by the determination of infective dose-50 in in vivo study. The objective of this study is to determinate ID50 of Avian Pathogenic Escherichia coli (APEC) in chicken and is to find out the histopathological image of chicken�s heart and liver after infected with that dose. In this study were used 42 of day old chicken (DOC) regardless their sex types. Those chickens were kept until 14 days old then were infected with APEC. The infective dose-50 was determined by Reed and Muench method. Isolation and identification of E.coli from heart and liver was cultured on eosin methylene blue (EMB) and mac Conkey agar (MCA). Biochemical reaction was tested with triple sugar iron agar (TSIA), Indole-Methyl red-Voges Proskauer-Citrate (IMVIC) test and Urea agar. Histopathological image of heart and liver was observed. The results of this study is infective dose of APEC in broiler chicken was 2 x 104,4 cell per ml (cell/ml). This dose caused damage...
Buletin Fakultas Peternakan Universitas Gadjah Mada/Buletin Peternakan, May 31, 2024
Phospholipase in buck seminal plasma will trigger a coagulation incident resulting in the demise ... more Phospholipase in buck seminal plasma will trigger a coagulation incident resulting in the demise of the spermatozoa during liquid semen processing using milk or egg yolk substrates diluent. A plasma replacement process is performed to avoid this possibility. The cryopreservation process leads to sperm cell damage due to the freezing process, therefore cryoprotectant agents such as saccharides are required as protective agents. This study aimed to investigate the effect of various types of saccharides on the quality of frozen semen of etawah crossbreed (PE) bucks during cryopreservation and thawing with plasma replacement using Priangan ram semen plasma. Semen was collected using an artificial vagina once a week. Fresh PE bucks semen centrifuged at 3,000 RPM for 30 min. The supernatant (seminal plasma) was removed and replaced in equal volume with Priangan ram seminal plasma. Semen was divided into four treatments: goat semen with sheep semen plasma in Tris diluent (control); control with 0.6% dextrose added (monosaccharides); control with 0.6% sucrose added (disaccharides); control with 0.6% raffinose added (trisaccharides). Semen quality including percentages of motile spermatozoa, live spermatozoa, and intact plasma membrane (IPM) were evaluated after diluted, equilibrated, and thawing, respectively. Results of this study showed that viability and IPM of monosaccharides, disaccharides, and trisaccharides were significantly (P<0.05) higher than control (54.40 and 51.40; 55.00 and 53.60; 55.60 and 52.20 vs 48.40 and 52.20, respectively). The motility of disaccharides was significantly (P<0.05) higher compared to control and other diluent (47.00 vs 41.00, respectively). In conclusion, different types of saccharides were effective in maintaining the quality of etawah crossbreed buck frozen semen. The addition of disaccharides seems more effective compared to monosaccharides and trisaccharides in tris extender on the quality of etawah crossbreed buck frozen semen.
Advances in Animal and Veterinary Sciences, Dec 31, 2022
The objective of this study was to compare the effects of enzymatic (EZ) and non-enzymatic (NEZ) ... more The objective of this study was to compare the effects of enzymatic (EZ) and non-enzymatic (NEZ) antioxidants on the quality of spermatozoa in chilled, stored liquid semen from Garut rams. The rams were randomly divided into seven treatments in a 2 × 3 factorial design. The two factors were the antioxidant levels of EZ (25, 50, and 75 mg) and NEZ (5, 10, and 20 mg). Motility, viability, and plasma membrane integrity were the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4 th day of observation showed that the addition of ascorbic acid as NEZ at a dose of 20 mg/100 ml into the egg yolk tris extender resulted in the greatest preservation of ram sperm quality (P<0.05), such as motility and viability. Hence, the addition of catalase as EZ at a dose of 50 mg/100 ml to the egg yolk tris extender resulted in the highest (P<0.05) percentage of motility, percentage of live spermatozoa, and intact plasma membrane. The NEZ group showed better results (P<0.05) than the NEZ group in maintaining motility, viability, and membrane integrity. The research concluded that the administration of NEZ at a dose of 20 mg/100 ml extender and EZ treatment at 50 mg/100 ml extender represents the optimal dose to preserve sperm quality until day four of dilution. In addition, the NEZ antioxidant showed better-improved quality of spermatozoa in the liquid semen of Garut rams compared to the EZ antioxidant.
Pathogenecity of Escherichia coli could be known by the determination of infective dose-50 in in ... more Pathogenecity of Escherichia coli could be known by the determination of infective dose-50 in in vivo study. The objective of this study is to determinate ID50 of Avian Pathogenic Escherichia coli (APEC) in chicken and is to find out the histopathological image of chicken�s heart and liver after infected with that dose. In this study were used 42 of day old chicken (DOC) regardless their sex types. Those chickens were kept until 14 days old then were infected with APEC. The infective dose-50 was determined by Reed and Muench method. Isolation and identification of E.coli from heart and liver was cultured on eosin methylene blue (EMB) and mac Conkey agar (MCA). Biochemical reaction was tested with triple sugar iron agar (TSIA), Indole-Methyl red-Voges Proskauer-Citrate (IMVIC) test and Urea agar. Histopathological image of heart and liver was observed. The results of this study is infective dose of APEC in broiler chicken was 2 x 104,4 cell per ml (cell/ml). This dose caused damage...
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