Presentamos los primeros resultados de los trabajos de prospección llevados a cabo en el Parque N... more Presentamos los primeros resultados de los trabajos de prospección llevados a cabo en el Parque Natural de los Valles Occidentales (Huesca) entre 2016 y 2019 que han concluido con la inclusión de 52 nuevos monumentos prehistóricos en el inventario del Gobierno de Aragón. Sumado al ya de por sí numeroso registro megalítico del que disponíamos, las nuevas incorporaciones nos permiten hacernos una idea bastante completa de la distribución de estos monumentos en el Parque Natural. En este trabajo defendemos la oportunidad que puede suponer estudiar este tipo de registro arqueológico para comprender mejor la humanización de la montaña pirenaica durante la Prehistoria. Así, la distribución espacial de monumentos megalíticos responde a una serie de lógicas de las sociedades que los construyeron y se relacionan con un cambio de racionalidad a la hora entender el paisaje que habitaban. Proponemos que estudiar qué tipo de espacios decidieron monumentalizarse y las estrategias llevadas a cabo para ello puede servirnos para enriquecer el relato histórico de la humanización del Pirineo que tanto se ha desarrollado en los últimos años.
Background: Factors influencing the development of alloantibodies against blood group antigens on... more Background: Factors influencing the development of alloantibodies against blood group antigens on transfused red blood cells are poorly defined. We hypothesised that transfused platelets may act as a danger signal to recipients and affect humoral immune responses to transfused red blood cells. Materials and methods: Platelet-rich plasma prepared from wild-type C57BL/6 or CD40L knock-out donors was transfused into wild-type or CD40L knock-out recipients. Leucoreduced red blood cells from transgenic donors expressing high levels of the human KEL glycoprotein in an erythrocyte-specific manner (KELhi donors) were transfused after the platelets, and anti-KEL responses were measured longitudinally. In some experiments, recipients were treated with poly (I:C), monoclonal CD40L-blocking antibody, or CD4-depleting antibody prior to transfusion. Results: Transfusion of wild-type C57BL/6 platelets or treatment with poly (I:C) prior to KELhi red blood cell transfusion led to an anti-KEL alloimmune response in wild-type recipients. Transfusion of platelets from wild-type but not CD40L knock-out donors prior to KELhi red blood cell transfusion led to an IgG anti-KEL alloimmune response in CD40L knock-out recipients; unexpectedly, transfusion of platelets from CD40L knock-out donors prior to KELhi red blood cell transfusion led to a robust anti-KEL alloimmune response in wild-type recipients. Recipient treatment with MR1 CD40L-blocking antibody or CD4-depleting antibody prevented KEL alloimmunisation altogether. Discussion: Transfused platelets serve as an adjuvant in this T-dependent murine model of anti-KEL red blood cell alloimmunisation, with CD40/CD40L interactions being involved to some degree but with additional mechanisms also playing a role. These findings raise questions about the role that transfused or endogenous platelets may play in other innate/adaptive immune responses.
Este artículo muestra teórica y prácticamente un protocolo para el uso de los recursos fotogramét... more Este artículo muestra teórica y prácticamente un protocolo para el uso de los recursos fotogramétricos en modelado tridimensional arqueológico. De este modo y de una forma relativamente asequible, es posible compilar, sistematizar, utilizar y compartir los archivos generados-fotogramétricos y 3D-tanto para trabajar con hipótesis, como para universalizar el conocimiento, ya sea con repositorios en red para especialistas o para facilitar la didáctica y difusión de la Historia y la Arqueología. Como ejemplo se exponen las posibilidades y problemas intrínsecos detectados en su aplicación al edificio columnado de Jamila (Villanueva de los Infantes, Ciudad Real, España). Este yacimiento es ideal para poner en práctica dicho protocolo en tanto que aúna la vocación divulgadora del equipo que lo estudia, con la falta de documentación en sus primeras campañas y una compleja interpretación histórico-arqueológica al tratarse de un lugar con distintas reocupaciones, algunas de ellas únicas en su tipología.
HAL (Le Centre pour la Communication Scientifique Directe), 2019
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Distributed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License Rituels invisibles. L'espace rituel protohistorique de Mata del Fraile
Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departam... more Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 17-11-2001CD69 is a C-type lectin early induced following activation of leukocytes. Structurefunction relationship studies have shown that the ligand binding and the signal transduction domains of CD69 and snother C-type lectin, CD23, function independently and the signal transduced depends on the cytoplasmic tail. In other studies about CD69 induction, we found that interaction of the T lymphocyte with endothelium h g h the LFA-I/lCAM-1 palhway increases CD69 expression. This effect is additive to tite stimulation provided by L-15. To investigate the possible role of CD69 in viva, CD69 deficient mice were generated and analyzed. Positive and negative selection at thymus was not affected CD69 deficiency leaded to a sligbt increment in pre-B ceh at bone marrow and a significant increase in the Tdependent B ceU response. CD69 is highly expressed by leukocytes at intiammatory sites, but its physiological mle during inflammation remains unlaiown. The tole of CD69 in the autoimmune reactivity was explored in a model of collagen-induced arthntis (CIA) in wild type and CD69-deficient mice. CD69 mice showed higher incidence and severity of CIA. with exacerbated T and B immune responss to type 11 coilagen. Transf&g growth factor (TGF)-beta 1 and 2. which act as protective in CIA. were reduced inCD69 mice inflammatoq foci, correlating wilh an inmase in some pminflammatory cytolanes. Local injection of blocking anti-TGF-beta antibodies increased CIA severity and proinfiammatory cytoLine mRNA levels in ~ ~ 6 9 + /b+ut, not in ~ ~ 6 m9ic"e. In addition, the treatment with anti-CD69 blocking antibodies in viva confinned the negative regulatory role of CD69 through n;F-be@-related mechanisms in an independent CIA model using DBAII mice. Moreover, in v&ra engagement of CD69 induced mtal and active TGF-betal prcduction. Our resulis show that CD69 is a negative modulator of autoimmune reactivity aod iaflamrmrtion h g b th e synibesis of TGF-beta, a cytokine thai in tun~do wn-regulates the pmduuion of different pro-inflammatory mediatas
Los dispositivos defensivos de los núcleos fortificados de la Edad del Hierro del norte de la Pen... more Los dispositivos defensivos de los núcleos fortificados de la Edad del Hierro del norte de la Península Ibérica presentan elementos comunes con los de otros núcleos protohistóricos del occidente de Europa. En las últimas décadas se ha avanzado en la identificación de este tipo de estructuras, tanto en oppida, como en castros y en otros hábitats de menor tamaño. Junto a distintas tipologías de murallas en piedra con foso, en los últimos años se han identificado también complejos sistemas de multivallado que llegan a englobar enormes superficies. En este trabajo se propone una clasificación elemental de las defensas protohistóricas del ámbito cantábrico y su comparación con otros territorios próximos de la fachada atlántica. Como caso concreto de estudio se profundiza en los dispositivos defensivos del oppidum de Monte Bernorio. Finalmente se realiza una lectura económica, social y simbólica de las fortificaciones de la Edad del Hierro.
espanolLa existencia de estructuras rituales de epoca prehistorica y protohistorica en espacios d... more espanolLa existencia de estructuras rituales de epoca prehistorica y protohistorica en espacios de montana es un fenomeno caracteristico de diversas zonas del area can-tabrica. En el presente articulo se analiza el conjunto de Mata del Fraile, una zona tumular situada en el entorno del oppidum de Monte Bernorio. La reciente excava-cion de dos tumulos ha proporcionado unicamente un cipo como toda evidencia arqueologica asociada a las estructuras. Tomando estos resultados como punto de partida, se ofrece una interpretacion de estas estructuras como espacios rituales que difieren de enterramientos propiamente dichos. EnglishThe existence of pre- and protohistoric ritual structures in highland locations is a characteristic phenomenon in several areas of the Cantabrian region. This paper analyses the evidence from Mata del Fraile, a group of tumuli located in the hinter-lands of the oppidum of Monte Bernorio. The recent excavation of two barrows has provided one stele as the only archaeo...
L'invention concerne des methodes permettant de traiter des sujets souffrant de troubles ou d... more L'invention concerne des methodes permettant de traiter des sujets souffrant de troubles ou d'etats caracterises par une reponse immunitaire indesirable, qui consiste a administrer une quantite effficace d'un agoniste de molecule d'activation precoce, un antagoniste ou un agent de depletion au sujet. L'invention concerne egalement des anticorps monoclonaux humains specifiques des molecules d'activation precoce et des methodes d'utilisation.
Teleost fish have been traditionally considered primitive vertebrates compared to mammals and bir... more Teleost fish have been traditionally considered primitive vertebrates compared to mammals and birds in regard to brain complexity and behavioral functions. However, an increasing amount of evidence suggests that teleosts show advanced cognitive capabilities including spatial navigation skills that parallel those of land vertebrates. Teleost fish rely on a multiplicity of sensory cues and can use a variety of spatial strategies for navigation, ranging from relatively simple body-centered orientation responses to allocentric or “external world-centered” navigation, likely based on map-like relational memory representations of the environment. These distinct spatial strategies are based on separate brain mechanisms. For example, a crucial brain center for egocentric orientation in teleost fish is the optic tectum, which can be considered an essential hub in a wider brain network responsible for the generation of egocentrically referenced actions in space. In contrast, other brain cente...
BACKGROUNDAlloantibodies to red blood cell (RBC) antigens can cause significant hemolytic events.... more BACKGROUNDAlloantibodies to red blood cell (RBC) antigens can cause significant hemolytic events. Prior studies have demonstrated that inflammatory stimuli in animal models and inflammatory states in humans, including autoimmunity and viremia, promote alloimmunization. However, molecular mechanisms underlying these findings are poorly understood. Given that Type 1 interferons (IFN‐α/β) regulate antiviral immunity and autoimmune pathology, the hypothesis that IFN‐α/β regulates RBC alloimmunization was tested in a murine model.STUDY DESIGN AND METHODSLeukoreduced murine RBCs expressing the human KEL glycoprotein were transfused into control mice (WT), mice lacking the unique IFN‐α/β receptor (IFNAR1–/–), or bone marrow chimeric mice lacking IFNAR1 on specific cell populations. Anti‐KEL IgG production, expressed as mean fluorescence intensity (MFI), and B‐cell differentiation were examined.RESULTSTransfused WT mice produced anti‐KEL IgG alloantibodies (peak response MFI, 50.4). However...
During RBC transfusion, production of alloantibodies against RBC non-ABO Ags can cause hemolytic ... more During RBC transfusion, production of alloantibodies against RBC non-ABO Ags can cause hemolytic transfusion reactions and limit availability of compatible blood products, resulting in anemia-associated morbidity and mortality. Multiple studies have established that certain inflammatory disorders and inflammatory stimuli promote alloimmune responses to RBC Ags. However, the molecular mechanisms underlying these findings are poorly understood. Type I IFNs (IFN-α/β) are induced in inflammatory conditions associated with increased alloimmunization. By developing a new transgenic murine model, we demonstrate that signaling through the IFN-α/β receptor is required for inflammation-induced alloimmunization. Additionally, mitochondrial antiviral signaling protein–mediated signaling through cytosolic pattern recognition receptors was required for polyinosinic-polycytidylic acid–induced IFN-α/β production and alloimmunization. We further report that IFN-α, in the absence of an adjuvant, is s...
Presentamos los primeros resultados de los trabajos de prospección llevados a cabo en el Parque N... more Presentamos los primeros resultados de los trabajos de prospección llevados a cabo en el Parque Natural de los Valles Occidentales (Huesca) entre 2016 y 2019 que han concluido con la inclusión de 52 nuevos monumentos prehistóricos en el inventario del Gobierno de Aragón. Sumado al ya de por sí numeroso registro megalítico del que disponíamos, las nuevas incorporaciones nos permiten hacernos una idea bastante completa de la distribución de estos monumentos en el Parque Natural. En este trabajo defendemos la oportunidad que puede suponer estudiar este tipo de registro arqueológico para comprender mejor la humanización de la montaña pirenaica durante la Prehistoria. Así, la distribución espacial de monumentos megalíticos responde a una serie de lógicas de las sociedades que los construyeron y se relacionan con un cambio de racionalidad a la hora entender el paisaje que habitaban. Proponemos que estudiar qué tipo de espacios decidieron monumentalizarse y las estrategias llevadas a cabo para ello puede servirnos para enriquecer el relato histórico de la humanización del Pirineo que tanto se ha desarrollado en los últimos años.
Background: Factors influencing the development of alloantibodies against blood group antigens on... more Background: Factors influencing the development of alloantibodies against blood group antigens on transfused red blood cells are poorly defined. We hypothesised that transfused platelets may act as a danger signal to recipients and affect humoral immune responses to transfused red blood cells. Materials and methods: Platelet-rich plasma prepared from wild-type C57BL/6 or CD40L knock-out donors was transfused into wild-type or CD40L knock-out recipients. Leucoreduced red blood cells from transgenic donors expressing high levels of the human KEL glycoprotein in an erythrocyte-specific manner (KELhi donors) were transfused after the platelets, and anti-KEL responses were measured longitudinally. In some experiments, recipients were treated with poly (I:C), monoclonal CD40L-blocking antibody, or CD4-depleting antibody prior to transfusion. Results: Transfusion of wild-type C57BL/6 platelets or treatment with poly (I:C) prior to KELhi red blood cell transfusion led to an anti-KEL alloimmune response in wild-type recipients. Transfusion of platelets from wild-type but not CD40L knock-out donors prior to KELhi red blood cell transfusion led to an IgG anti-KEL alloimmune response in CD40L knock-out recipients; unexpectedly, transfusion of platelets from CD40L knock-out donors prior to KELhi red blood cell transfusion led to a robust anti-KEL alloimmune response in wild-type recipients. Recipient treatment with MR1 CD40L-blocking antibody or CD4-depleting antibody prevented KEL alloimmunisation altogether. Discussion: Transfused platelets serve as an adjuvant in this T-dependent murine model of anti-KEL red blood cell alloimmunisation, with CD40/CD40L interactions being involved to some degree but with additional mechanisms also playing a role. These findings raise questions about the role that transfused or endogenous platelets may play in other innate/adaptive immune responses.
Este artículo muestra teórica y prácticamente un protocolo para el uso de los recursos fotogramét... more Este artículo muestra teórica y prácticamente un protocolo para el uso de los recursos fotogramétricos en modelado tridimensional arqueológico. De este modo y de una forma relativamente asequible, es posible compilar, sistematizar, utilizar y compartir los archivos generados-fotogramétricos y 3D-tanto para trabajar con hipótesis, como para universalizar el conocimiento, ya sea con repositorios en red para especialistas o para facilitar la didáctica y difusión de la Historia y la Arqueología. Como ejemplo se exponen las posibilidades y problemas intrínsecos detectados en su aplicación al edificio columnado de Jamila (Villanueva de los Infantes, Ciudad Real, España). Este yacimiento es ideal para poner en práctica dicho protocolo en tanto que aúna la vocación divulgadora del equipo que lo estudia, con la falta de documentación en sus primeras campañas y una compleja interpretación histórico-arqueológica al tratarse de un lugar con distintas reocupaciones, algunas de ellas únicas en su tipología.
HAL (Le Centre pour la Communication Scientifique Directe), 2019
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Distributed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License Rituels invisibles. L'espace rituel protohistorique de Mata del Fraile
Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departam... more Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 17-11-2001CD69 is a C-type lectin early induced following activation of leukocytes. Structurefunction relationship studies have shown that the ligand binding and the signal transduction domains of CD69 and snother C-type lectin, CD23, function independently and the signal transduced depends on the cytoplasmic tail. In other studies about CD69 induction, we found that interaction of the T lymphocyte with endothelium h g h the LFA-I/lCAM-1 palhway increases CD69 expression. This effect is additive to tite stimulation provided by L-15. To investigate the possible role of CD69 in viva, CD69 deficient mice were generated and analyzed. Positive and negative selection at thymus was not affected CD69 deficiency leaded to a sligbt increment in pre-B ceh at bone marrow and a significant increase in the Tdependent B ceU response. CD69 is highly expressed by leukocytes at intiammatory sites, but its physiological mle during inflammation remains unlaiown. The tole of CD69 in the autoimmune reactivity was explored in a model of collagen-induced arthntis (CIA) in wild type and CD69-deficient mice. CD69 mice showed higher incidence and severity of CIA. with exacerbated T and B immune responss to type 11 coilagen. Transf&g growth factor (TGF)-beta 1 and 2. which act as protective in CIA. were reduced inCD69 mice inflammatoq foci, correlating wilh an inmase in some pminflammatory cytolanes. Local injection of blocking anti-TGF-beta antibodies increased CIA severity and proinfiammatory cytoLine mRNA levels in ~ ~ 6 9 + /b+ut, not in ~ ~ 6 m9ic"e. In addition, the treatment with anti-CD69 blocking antibodies in viva confinned the negative regulatory role of CD69 through n;F-be@-related mechanisms in an independent CIA model using DBAII mice. Moreover, in v&ra engagement of CD69 induced mtal and active TGF-betal prcduction. Our resulis show that CD69 is a negative modulator of autoimmune reactivity aod iaflamrmrtion h g b th e synibesis of TGF-beta, a cytokine thai in tun~do wn-regulates the pmduuion of different pro-inflammatory mediatas
Los dispositivos defensivos de los núcleos fortificados de la Edad del Hierro del norte de la Pen... more Los dispositivos defensivos de los núcleos fortificados de la Edad del Hierro del norte de la Península Ibérica presentan elementos comunes con los de otros núcleos protohistóricos del occidente de Europa. En las últimas décadas se ha avanzado en la identificación de este tipo de estructuras, tanto en oppida, como en castros y en otros hábitats de menor tamaño. Junto a distintas tipologías de murallas en piedra con foso, en los últimos años se han identificado también complejos sistemas de multivallado que llegan a englobar enormes superficies. En este trabajo se propone una clasificación elemental de las defensas protohistóricas del ámbito cantábrico y su comparación con otros territorios próximos de la fachada atlántica. Como caso concreto de estudio se profundiza en los dispositivos defensivos del oppidum de Monte Bernorio. Finalmente se realiza una lectura económica, social y simbólica de las fortificaciones de la Edad del Hierro.
espanolLa existencia de estructuras rituales de epoca prehistorica y protohistorica en espacios d... more espanolLa existencia de estructuras rituales de epoca prehistorica y protohistorica en espacios de montana es un fenomeno caracteristico de diversas zonas del area can-tabrica. En el presente articulo se analiza el conjunto de Mata del Fraile, una zona tumular situada en el entorno del oppidum de Monte Bernorio. La reciente excava-cion de dos tumulos ha proporcionado unicamente un cipo como toda evidencia arqueologica asociada a las estructuras. Tomando estos resultados como punto de partida, se ofrece una interpretacion de estas estructuras como espacios rituales que difieren de enterramientos propiamente dichos. EnglishThe existence of pre- and protohistoric ritual structures in highland locations is a characteristic phenomenon in several areas of the Cantabrian region. This paper analyses the evidence from Mata del Fraile, a group of tumuli located in the hinter-lands of the oppidum of Monte Bernorio. The recent excavation of two barrows has provided one stele as the only archaeo...
L'invention concerne des methodes permettant de traiter des sujets souffrant de troubles ou d... more L'invention concerne des methodes permettant de traiter des sujets souffrant de troubles ou d'etats caracterises par une reponse immunitaire indesirable, qui consiste a administrer une quantite effficace d'un agoniste de molecule d'activation precoce, un antagoniste ou un agent de depletion au sujet. L'invention concerne egalement des anticorps monoclonaux humains specifiques des molecules d'activation precoce et des methodes d'utilisation.
Teleost fish have been traditionally considered primitive vertebrates compared to mammals and bir... more Teleost fish have been traditionally considered primitive vertebrates compared to mammals and birds in regard to brain complexity and behavioral functions. However, an increasing amount of evidence suggests that teleosts show advanced cognitive capabilities including spatial navigation skills that parallel those of land vertebrates. Teleost fish rely on a multiplicity of sensory cues and can use a variety of spatial strategies for navigation, ranging from relatively simple body-centered orientation responses to allocentric or “external world-centered” navigation, likely based on map-like relational memory representations of the environment. These distinct spatial strategies are based on separate brain mechanisms. For example, a crucial brain center for egocentric orientation in teleost fish is the optic tectum, which can be considered an essential hub in a wider brain network responsible for the generation of egocentrically referenced actions in space. In contrast, other brain cente...
BACKGROUNDAlloantibodies to red blood cell (RBC) antigens can cause significant hemolytic events.... more BACKGROUNDAlloantibodies to red blood cell (RBC) antigens can cause significant hemolytic events. Prior studies have demonstrated that inflammatory stimuli in animal models and inflammatory states in humans, including autoimmunity and viremia, promote alloimmunization. However, molecular mechanisms underlying these findings are poorly understood. Given that Type 1 interferons (IFN‐α/β) regulate antiviral immunity and autoimmune pathology, the hypothesis that IFN‐α/β regulates RBC alloimmunization was tested in a murine model.STUDY DESIGN AND METHODSLeukoreduced murine RBCs expressing the human KEL glycoprotein were transfused into control mice (WT), mice lacking the unique IFN‐α/β receptor (IFNAR1–/–), or bone marrow chimeric mice lacking IFNAR1 on specific cell populations. Anti‐KEL IgG production, expressed as mean fluorescence intensity (MFI), and B‐cell differentiation were examined.RESULTSTransfused WT mice produced anti‐KEL IgG alloantibodies (peak response MFI, 50.4). However...
During RBC transfusion, production of alloantibodies against RBC non-ABO Ags can cause hemolytic ... more During RBC transfusion, production of alloantibodies against RBC non-ABO Ags can cause hemolytic transfusion reactions and limit availability of compatible blood products, resulting in anemia-associated morbidity and mortality. Multiple studies have established that certain inflammatory disorders and inflammatory stimuli promote alloimmune responses to RBC Ags. However, the molecular mechanisms underlying these findings are poorly understood. Type I IFNs (IFN-α/β) are induced in inflammatory conditions associated with increased alloimmunization. By developing a new transgenic murine model, we demonstrate that signaling through the IFN-α/β receptor is required for inflammation-induced alloimmunization. Additionally, mitochondrial antiviral signaling protein–mediated signaling through cytosolic pattern recognition receptors was required for polyinosinic-polycytidylic acid–induced IFN-α/β production and alloimmunization. We further report that IFN-α, in the absence of an adjuvant, is s...
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