Papers by claire vinatier
1. IntroductionJoint cartilage is a specialized connective tissue thatcontains a single cell type... more 1. IntroductionJoint cartilage is a specialized connective tissue thatcontains a single cell type, the chondrocyte, and a highlyhydrated extracellular matrix composed chiefly of collagensand glycosaminoglycans. There are no blood vessels or nerves.The composition and histological architecture of joint carti-lage ensures the transmission and distribution of mechanicalloads through the joint. Joint cartilage can be altered byinjuries, inflammatory disease (e.g., rheumatoid arthritis), ordegenerative disease (osteoarthritis). Because there is novascular supply, and because chondrocytes cannot divide, jointcartilage has limited capacity for self-healing, so thatdestructive lesions are irreversible. For many years,researchers have sought means of regenerating functional jointcartilage. Numerous approaches have been developed topromote cartilage repair, and interest in biomaterials and celltherapy is growing among clinicians [1]. An appropriatematrix can be used in combination with growth f...
Joint Bone Spine, 2021
Osteoarthritis affects hundreds of millions of people worldwide, and its prevalence is constantly... more Osteoarthritis affects hundreds of millions of people worldwide, and its prevalence is constantly increasing. While there is currently no treatment that can alter the course of the disease, promising therapeutic strategies and novel targets are being investigated. Innovative cell therapies are already reaching clinical trials, and recent progress in our understanding of the disease is opening new routes for gene therapy. In the long term, the development of new biofabrication tools, such as 3D bioprinting, may pave the way for personalized mini-joint models that could be used to screen drugs and to personalize treatments. This review provides an overview of the most promising therapeutic approaches in the field of osteoarthritis, from upcoming treatments to those that are yet to be discovered.
Biotechnology Advances, 2018
Current protocols for chondrocyte expansion and chondrogenic differentiation of stem cells fail t... more Current protocols for chondrocyte expansion and chondrogenic differentiation of stem cells fail to reduce phenotypic loss and to mitigate hypertrophic tendency. To this end, cell genetic manipulation is gaining pace as a means of generating cells with stable chondrocyte phenotype. Herein, we provide an overview of candidate genes that either induce cartilage regeneration or inhibit cartilage degeneration. We further discuss in vitro, ex vivo and in vivo viral transduction and non-viral transfection strategies for targeted cells (chondrocytes, mesenchymal stem cells, induced pluripotent stem cells and synovial cells), along with the most representative results obtained in pre-clinical models and in clinical trials. We highlight current challenges and associated risks that slowdown clinical acceptance and commercialisation of gene transfer technologies.
Scientific Reports, 2016
Polyphenols exert a large range of beneficial effects in the prevention of age-related diseases. ... more Polyphenols exert a large range of beneficial effects in the prevention of age-related diseases. We sought to determine whether an extract of olive and grape seed standardized according to hydroxytyrosol (HT) and procyanidins (PCy) content, exerts preventive anti-osteoathritic effects. To this aim, we evaluated whether the HT/PCy mix could (i) have in vitro anti-inflammatory and chondroprotective actions, (ii) exert anti-osteoarthritis effects in two post-traumatic animal models and (iii) retain its bioactivity after oral administration. Anti-inflammatory and chondroprotective actions of HT/PCy were tested on primary cultured rabbit chondrocytes stimulated by interleukin-1 beta (IL-1β). The results showed that HT/PCy exerts anti-inflammatory and chondroprotective actions in vitro. The preventive effect of HT/PCy association was assessed in two animal models of post-traumatic OA in mice and rabbits. Diet supplementation with HT/PCy significantly decreased the severity of post-traumat...
Drug Discovery Today, 2016
Osteoarthritis (OA) is a degenerative joint disease that, despite recent progress, has no curativ... more Osteoarthritis (OA) is a degenerative joint disease that, despite recent progress, has no curative treatment. Considerable research has recently been initiated to identify new potential therapeutic targets. In this review, we will set forth some of the major discoveries in the past 5 years, notably those dealing with the identification of pathogenic factors [hypoxia-inducible factors (HIFs), complement, transforming growth factor (TGF)-β and zinc-ZIP8]. New drugs and concepts currently in clinical development [anti-nerve growth factor (NGF), mesenchymal stromal cells and fibroblast growth factor (FGF)-18] will then be addressed. Finally, we will consider prospective avenues that could lead to mid-to-long-term developments of novel therapeutic concepts, notably those dealing with autophagy regulation and induced pluripotent stem cells.
Revue du Rhumatisme, 2008
Colloque de recherche Cellules souches mésenchymateuses du tissu adipeux et biomatériaux pour l'i... more Colloque de recherche Cellules souches mésenchymateuses du tissu adipeux et biomatériaux pour l'ingénierie tissulaire du cartilage ଝ
Cellular Physiology and Biochemistry, 2015
Background/Aims: Multipotent stem/stromal cells (MSC) are considered promising for cartilage tiss... more Background/Aims: Multipotent stem/stromal cells (MSC) are considered promising for cartilage tissue engineering. However, chondrogenic differentiation of MSC can ultimately lead to the formation of hypertrophic chondrocytes responsible for the calcification of cartilage. To prevent the production of this calcified matrix at the articular site, the late hypertrophic differentiation of MSCs must be carefully controlled. Given that articular cartilage is avascular, we hypothesized that in addition to its stimulatory role in the early differentiation of chondrogenic cells, hypoxia may prevent their late hypertrophic conversion. Methods: Early and late chondrogenic differentiation were evaluated using human adipose MSC and murine ATDC5 cells cultured under either normoxic (21%O2) or hypoxic (5%O2) conditions. To investigate the effect of hypoxia on late chondrogenic differentiation, the transcriptional activity of hypoxia-inducible factor-1alpha (HIF-1α) and HIF-2α were evaluated using t...
Osteoarthritis and Cartilage, 2014
Revue du Rhumatisme, 2009
Trends in Molecular Medicine, 2005
Trends in Biotechnology, 2009
Injuries to articular cartilage are one of the most challenging issues of musculoskeletal medicin... more Injuries to articular cartilage are one of the most challenging issues of musculoskeletal medicine due to the poor intrinsic ability of this tissue for repair. The lack of efficient modalities of treatment has prompted research into tissue engineering combining chondrogenic cells, scaffold materials and environmental factors. The aim of this review is to focus on the recent advances made in exploiting the potential of biomaterial-assisted cell therapy for cartilage engineering. We discuss the requirements for identifying additional specific growth factors and evaluating the optimal combination of cells, growth factors and scaffolds that is able to respond to the functional demand placed upon cartilage tissue replacement in clinics. Finally, some of the major obstacles encountered in cartilage engineering are discussed, as well as future trends in clinical applications.
Marine Drugs, 2011
Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are grea... more Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared to the sterilization methods using gamma and beta radiations. The changes to both the physical and chemical properties of the sterilized exopolysaccharides were analyzed. The use of ethylene oxide can be recommended for the sterilization of polysaccharides as a weak effect on both rheological and structural properties was observed. This low-temperature gas sterilizing process is very efficient, giving a good Sterility Assurance Level (SAL), and is also well suited to large-scale compound manufacturing in the pharmaceutical industry.
Macromolecular Symposia, 2008
We have developed a self-reticulating polymer based on silanized hydroxypropylmethylcellulose (Si... more We have developed a self-reticulating polymer based on silanized hydroxypropylmethylcellulose (Si-HPMC). The aim of this study was to determine whether this Si-HPMC hydrogel with or without calcium phosphate granules could represent a potential scaffold for bone tissue engineering. This study showed that Si-HPMC hydrogel didn't affect SaOS-2 and rat bone marrow cells viability. In addition, SaOS-2 cells are able to proliferate within Si-HPMC hydrogel containing or not calcium phosphate granules whereas Rat bone marrow cells proliferate only at the surface of calcium phosphate granules contained within Si-HPMC hydrogel. Finally, SaOS-2 cells seeded at the surface of reticulated Si-HPMC were not able to penetrate the hydrogel, while J774, a macrophage cells line, were able to move into the Si-HPMC hydrogel. These data indicate that Si-HPMC is a promissing scaffold for tissue engineering.
Joint Bone Spine, 2009
laboratoire d'ingénierie ostéoarticulaire et dentaire (LIOAD), « groupe physiopathologie des tiss... more laboratoire d'ingénierie ostéoarticulaire et dentaire (LIOAD), « groupe physiopathologie des tissus squelettiques et ingénierie du cartilage »,
Joint Bone Spine, 2008
d'ingénierie des tissus ostéoarticulaires et dentaires, équipe ''physiopathologie des tissus sque... more d'ingénierie des tissus ostéoarticulaires et dentaires, équipe ''physiopathologie des tissus squelettiques et ingénierie du cartilage'',
Drug Discovery Today, 2009
Osteoarthritis (OA) is associated with cartilage degeneration and an accompanying inflammatory sy... more Osteoarthritis (OA) is associated with cartilage degeneration and an accompanying inflammatory syndrome of the synovium in addition to alteration of the subchondral bone. The molecular and cellular events involved in OA have only partially been elucidated. This review provides a global view of the physiopathology of OA, as well as non-pharmacological and pharmacological treatments for the disorder. An update on surgical treatments and their indications is given with an orientation towards the management of OA and cartilage repair by cell-based regenerative therapies. These promising biological technologies will, potentially, play a major role in the treatment of cartilage-associated diseases. Osteoarthritis (OA) is a public health concern particularly in modern society and is the leading osteoarticular pathology of developed countries. In the United States, OA is the primary reason for medical consultation in persons older than 60 years of age and affects at least 30% of this subpopulation [1]. Population ageing will probably worsen the socioeconomic impact of such pathologies. The growing epidemic of obesity is also an exacerbating factor, with an indisputable role in knee OA [2]. The current view is that OA is a complex syndrome that is, in fact, the ultimate outcome of various factors affecting the joint [3]. Once established, OA is characterised by a decrease in articular cartilage (AC) thickness, subchondral bone sclerosis (bone thickening), formation of osteophytes (bone outgrowth on the joint margin) and modification of the synovial fluid composition (Fig. 1). Several joints might be affected by OA but the sites most commonly affected are knees, hips, fingers and the lumbar and cervical spine. Given that many questions, particularly those concerning the physiopathology of OA, remain unanswered, it is not surprising that treatments, either pharmacological or surgical, only partially address the clinical issue. Reviews KEYNOTE REVIEW Johann Clouet was born in Châteaubriant, France, on 26 November 1975. He obtained his doctorate degree in pharmacy in 2006 and his research master's degree in 2007 at the University of Nantes, (France). Presently he is working in the department of physiopathology of skeletal tissues and cartilage engineering in the centre for osteoarticular and dental tissue engineering (LIOAD-INSERM U791) at the University of Nantes (France). His research work focuses predominantly on the development of scaffolds and the use of stem cells for cartilage tissue engineering. Jé rô me Guicheux was born in Le Mans (France) on 20 February 1970. He obtained his PhD thesis in 1997 and his degree for directing research in 2002. Currently, he is working as a research director/professor at the national institute for health and medical research (INSERM). He is head of the department of physiopathology of skeletal tissues and cartilage engineering in the centre for osteoarticular and dental tissue engineering (LIOAD-INSERM U791) at the University of Nantes (France). His research focuses on the differentiation process of osteoarticular cells and the use of scaffolds and reparative cells for cartilage tissue engineering.
Bone, 2011
Osteoarthritis (OA) is a progressive degenerative disease of joints, causing pain and disability.... more Osteoarthritis (OA) is a progressive degenerative disease of joints, causing pain and disability. Although all joint tissues are affected, changes in articular cartilage are predominant. Recent studies have shown that cannabinoid receptors are expressed in bone and the endocannabinoid system may be involved in the regulation of bone turnover. Two, cannabinoid receptors have been identified; CB1 and CB2. A third receptor, GPR55, also binds to certain cannabinoid agonists. The aim of this study was undertaken to assess whether functional cannabinoid receptors were expressed by chondrocytes, the cells of cartilage, and to establish whether they may have a role in osteoarthritis. Expression of CB1, CB2 and GPR55 receptors by normal and OA chondrocytes was assessed by RT-PCR and Western blotting. To investigate potential functions of the receptors, chondrocytes were stimulated with specific CB1 and CB2 agonists and phosphorylation of Akt, and the MAPkinases ERK and p38 was investigated. Normal chondrocytes expressed CB2 and GPR55 receptors, and OA chondrocytes showed expression of all three receptors. Normal chondrocytes stimulated by the CB1 and CB2 agonists did not show up-regulation of the signaling pathways investigated. Stimulation of OA chondrocytes with a CB2 agonist whilst showing no effect on Akt or ERK phosphorylation increased p38 phosphorylation. These studies show that chondrocytes derived from normal and OA human articular cartilage express cannabinoid receptors but potential roles in regulating pathological processes in OA remain to be defined. This article of a Special Issue entitled ECTS 2011. Disclosure of interest: None declared.
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Papers by claire vinatier