Feline mammary cancers have high recurrence and metastatic potential. Abnormal expression or func... more Feline mammary cancers have high recurrence and metastatic potential. Abnormal expression or function in the major molecules of the cadherin-catenin adhesion complex have been related to breast cancer development and associated to cell migration, invasion and metastatic dissemination. In feline mammary tumours, cadherins and catenins´ role is still poorly known. Therefore, we seek for suitable in vitro and in vivo model systems to study the leading role of P-cadherin and the molecules of the cadherin-catenin complex (CCC) in feline mammary carcinogenesis. Major molecules from the CCC (E-and P-cadherin, α-, β-and p120-catenin) were evaluated in a feline mammary carcinoma cell line (FMCm), by Western blot analysis, immunofluorescence, imunoprecipitation and in situ proximity ligation assay. The FMCm cell line tumourigenic and metastatic capacity was assessed by orthotopically inoculation of a cell suspension in the mammary fat pad of athymic nude mice (N:NIH (S) II-nu/nu). Mice xenografts, as well as metastatic lesions, were evaluated immunohistochemically for cadherins and catenins expression. P-and E-cadherin double-labelling immunofluorescence was also assessed. The FMCm cell line expressed E-and P-cadherin as well as α-, β-and p120-catenin. E-cadherin was showed to interact with each catenin, revealing a putative complete CCC. The cells had E-and P-cadherin co-expression and a close proximity between these two molecules. The FMCm cell line revealed to be highly tumourigenic and metastatic leading to the formation of primary and metastatic lesions in all animals that express all the molecules from the CCC studied. FMCm cell line represents a useful model for in vitro and in vivo studies of feline mammary carcinoma progression.
The search for molecular markers in the feline mammary gland, namely, the adhesion molecules belo... more The search for molecular markers in the feline mammary gland, namely, the adhesion molecules belonging to the cadherin family, is useful in the understanding of the development of mammary carcinomas in felines and humans. To study P-cadherin expression in the feline mammary gland, 61 samples of normal (n=4), hyperplastic (n=12), and neoplastic (n=45) feline mammary tissues were examined. In both normal and hyperplastic mammary tissues as well as in benign tumours, P-cadherin immunolabelling was restricted to myoepithelial cells. In malignant tumours, however, there was an aberrant epithelial P-cadherin immunoexpression in 64.1% (n=25) of cases, with a membranous and/or cytoplasmic pattern of distribution. A statistically significant relationship was seen between epithelial P-cadherin expression and malignant mammary lesions (P=0.0001). In malignant mammary tumours, there was likewise a statistically significant relationship between aberrant P-cadherin immunoexpression and histologic...
BackgroundCadherins are calcium-dependent cell-to-cell adhesion glycoproteins playing a critical ... more BackgroundCadherins are calcium-dependent cell-to-cell adhesion glycoproteins playing a critical role in the formation and maintenance of normal tissue architecture. In normal mammary gland, E-cadherin is expressed by luminal epithelial cells, while P-cadherin is restricted to myoepithelial cells. Changes in the expression of classical E- and P-cadherins have been observed in mammary lesions and related to mammary carcinogenesis. P-cadherin and E-cadherin expressions were studied in a series of feline normal mammary glands, hyperplastic/dysplastic lesions, benign and malignant tumours by immunohistochemistry and double-label immunofluorescence.ResultsIn normal tissue and in the majority of hyperplastic/dysplastic lesions and benign tumours, P-cadherin was restricted to myoepithelial cells, while 80% of the malignant tumours expressed P-cadherin in luminal epithelial cells. P-cadherin expression was significantly related to high histological grade of carcinomas (p <0.0001), tumour...
Feline mammary cancers have high recurrence and metastatic potential. Abnormal expression or func... more Feline mammary cancers have high recurrence and metastatic potential. Abnormal expression or function in the major molecules of the cadherin-catenin adhesion complex have been related to breast cancer development and associated to cell migration, invasion and metastatic dissemination. In feline mammary tumours, cadherins and catenins´ role is still poorly known. Therefore, we seek for suitable in vitro and in vivo model systems to study the leading role of P-cadherin and the molecules of the cadherin-catenin complex (CCC) in feline mammary carcinogenesis. Major molecules from the CCC (E-and P-cadherin, α-, β-and p120-catenin) were evaluated in a feline mammary carcinoma cell line (FMCm), by Western blot analysis, immunofluorescence, imunoprecipitation and in situ proximity ligation assay. The FMCm cell line tumourigenic and metastatic capacity was assessed by orthotopically inoculation of a cell suspension in the mammary fat pad of athymic nude mice (N:NIH (S) II-nu/nu). Mice xenografts, as well as metastatic lesions, were evaluated immunohistochemically for cadherins and catenins expression. P-and E-cadherin double-labelling immunofluorescence was also assessed. The FMCm cell line expressed E-and P-cadherin as well as α-, β-and p120-catenin. E-cadherin was showed to interact with each catenin, revealing a putative complete CCC. The cells had E-and P-cadherin co-expression and a close proximity between these two molecules. The FMCm cell line revealed to be highly tumourigenic and metastatic leading to the formation of primary and metastatic lesions in all animals that express all the molecules from the CCC studied. FMCm cell line represents a useful model for in vitro and in vivo studies of feline mammary carcinoma progression.
The search for molecular markers in the feline mammary gland, namely, the adhesion molecules belo... more The search for molecular markers in the feline mammary gland, namely, the adhesion molecules belonging to the cadherin family, is useful in the understanding of the development of mammary carcinomas in felines and humans. To study P-cadherin expression in the feline mammary gland, 61 samples of normal (n=4), hyperplastic (n=12), and neoplastic (n=45) feline mammary tissues were examined. In both normal and hyperplastic mammary tissues as well as in benign tumours, P-cadherin immunolabelling was restricted to myoepithelial cells. In malignant tumours, however, there was an aberrant epithelial P-cadherin immunoexpression in 64.1% (n=25) of cases, with a membranous and/or cytoplasmic pattern of distribution. A statistically significant relationship was seen between epithelial P-cadherin expression and malignant mammary lesions (P=0.0001). In malignant mammary tumours, there was likewise a statistically significant relationship between aberrant P-cadherin immunoexpression and histologic...
BackgroundCadherins are calcium-dependent cell-to-cell adhesion glycoproteins playing a critical ... more BackgroundCadherins are calcium-dependent cell-to-cell adhesion glycoproteins playing a critical role in the formation and maintenance of normal tissue architecture. In normal mammary gland, E-cadherin is expressed by luminal epithelial cells, while P-cadherin is restricted to myoepithelial cells. Changes in the expression of classical E- and P-cadherins have been observed in mammary lesions and related to mammary carcinogenesis. P-cadherin and E-cadherin expressions were studied in a series of feline normal mammary glands, hyperplastic/dysplastic lesions, benign and malignant tumours by immunohistochemistry and double-label immunofluorescence.ResultsIn normal tissue and in the majority of hyperplastic/dysplastic lesions and benign tumours, P-cadherin was restricted to myoepithelial cells, while 80% of the malignant tumours expressed P-cadherin in luminal epithelial cells. P-cadherin expression was significantly related to high histological grade of carcinomas (p <0.0001), tumour...
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