Papers by amornrat chookliang
The key component of the skin pigmentation is melanocytes due to ability in melanin production. P... more The key component of the skin pigmentation is melanocytes due to ability in melanin production. Pigmentation disorders are more common in the diabetes mellitus (DM), especially hyperpigmentation. It is caused by an increased number or activity of the melanocytes. The present study aimed to investigate the effects of long-term DM, chemically induced by streptozotocin (STZ), on the pathological changes of melanocytes in the rat skin. Seven adult male Sprague-Dawley rats were randomly divided into two groups: non-diabetic control and STZ-induced diabetic groups. In the diabetic rats, an increased amount of melanocytes at the dermoepidermal junction with increased melanin granules in the basal layer of keratinocytes were demonstrated. Moreover, the diabetic melanocytes became hypertrophy and presence of large clear area in their cytoplasm around the nucleus. It was suggested that diabetes caused hyperpigmentation. Its potential mechanism will be investigated in the future research.
Scientific Research and Essays, 2015
A chronic complication of diabetes mellitus (DM), diabetic neuropathy, causes neurodegeneration l... more A chronic complication of diabetes mellitus (DM), diabetic neuropathy, causes neurodegeneration leading to the development of neuropathic pain and sensation loss, particularly in the hands and arms. The cervical enlargement of the spinal cord sends the nerve supply to the hands and arms. Moreover, astrocytes effectively provide structural and functional maintenance of neurons in the spinal cord. Nevertheless, there is very little research concerning the ultrastructural changes of diabetic spinal astrocytes in the cervical enlargement. Thus, in this study, the ultrastructural changes of protoplasmic and fibrous astrocytes in streptozotocin (STZ)-induced diabetic and control rats using transmission electron microscopy (TEM) were examined and compared. Thirteen male diabetic and six male control rats were intraperitoneally administered with either STZ in citrate buffer or only the buffer solution, respectively. Rats were sacrificed at four (short term) and twenty-four weeks (long term)...
Delayed wound healing is one of complications in the diabetic dermopathy, and potentially causes ... more Delayed wound healing is one of complications in the diabetic dermopathy, and potentially causes chronic ulceration and limb amputation. To investigate the effects of diabetes on the skin, male Sprague-Dawley rats were used. After 24 weeks of a single dose of streptozotocin administration, the skin was removed as part of the histological process to produce microscopic slides and was viewed under a light microscope. It was shown that the thickness of the epidermis in long-termed diabetic rats decreased. Additionally, in the long-termed diabetic group, hypertrophic keratinocytes and pyknotic melanocytes were also observed in the stratum basale. The size of keratinocyte and its nucleus in the stratum spinosum was smaller. In addition, there was a dense-stained layer with shreds in the stratum corneum. It was concluded that long-termed diabetes causes these pathological aspects of the skin, and might further contribute to chronic wound formation. Therefore, skin problems should be manag...
Diabetic microangiopathy is a well-known cause of neuronal complications mainly in peripheral ner... more Diabetic microangiopathy is a well-known cause of neuronal complications mainly in peripheral nerves and brain. Additionally, it also strongly associates with the development of spinal infarction followed by the physical disability to seriously affected the quality of life in diabetic patients. However, only few study is known about the alterations of the spinal capillary in diabetes. Therefore, this study aimed to illustrate morphological changes of spinal capillaries in the early stage of streptozotocin (STZ)induced diabetic rat compared with control. In diabetes, the endothelial cell and pericyte became hypertrophy. Under the ultrastructural level, the endothelial cell contained a vast number of ribosomes and vesicles, enlarged mitochondria, and disrupted tight junction, while the vascular pericyte had numerous free ribosomes and a pair of centrioles. Furthermore, basement membrane thickening was detected. This basic knowledge will be beneficial for raised awareness and receiving...
International Journal of Morphology
Disturbances of sensory and motor nerve conduction velocity in the spinal cord as well as degener... more Disturbances of sensory and motor nerve conduction velocity in the spinal cord as well as degenerated myelin sheaths are observed in diabetic patients and animal models. Indeed, oligodendrocytes (OLs), which are important neuroglial cells, generate myelin in the central nervous system. Spinal enlargement, including cervical and lumbar enlargements, innervates all limbs. Thus, the purposes of this study were to examine and compare the ultrastructural alterations of OLs in spinal enlargements of streptozotocin (STZ)induced diabetic rats and controls. Thirteen male Sprague-Dawley rats were induced with STZ in citrate buffer and six control rats were injected with the same buffer solution. All rats were sacrificed after inductions at four (short-term DM) and twenty-four weeks (long-term DM). The selected spinal enlargements were processed for transmission electron microscopy. The OL alterations in both the cervical and lumbar enlargements were apparently the same. In short-term DM, the nuclei of OLs became swelled with chromatin clumping. Cytoplasmic organelles were moderately damaged. In long-term DM, OLs contained shrinkage nuclei with thick heterochromatin clumping. Severely degenerated mitochondria with disrupted cristae and broken membranes were observed. Moreover, distended and fragmented rough endoplasmic reticulum were observed, and large clear areas were present in the cytoplasm. Additionally, the loosening, splitting, and destruction of myelin lamellae were found. This study can provide important preliminary information about the alteration of OLs in the spinal cords of diabetic patients, which might be involve in the impairments of sensory and motor conduction velocities in these individuals.
International Journal of Morphology
Small bronchiolar histopathological changes related to prolonged diabetes.
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Papers by amornrat chookliang