Papers by agus syahrurachman
Journal of the Indonesian Medical Association, Mar 13, 2020
Pseudomonas aeroginosa dapat merupakan penyebab infeksi diberbagai organ. Pilihan antibiotika tun... more Pseudomonas aeroginosa dapat merupakan penyebab infeksi diberbagai organ. Pilihan antibiotika tunggal untuk itu menjadi lebih sedikit. Tujuan: Menilai daya antibakteri kombinasi fosfomisin dan sulbaktkam-sefoperazon in vitro terhadap isolat klinis Pseudomonas aeruginosa. Metode: Uji kerentanan 30 isolat klinis Pseudomonas aeruginosa terhadap fosfomisin dan sefoperazone-sulbactam sebagai obat tunggal dan kombinasinya dilakukan pada media cair. Daya antibakteri kombinasi fosmisin dan sulbactam sefoperazon ditentukan dengan menghitung Fraction Inhibition Index (FIC). Time-kill curve dinilai pasca paparan bakteri terhadap antibiotika selama waktu tertentu. Hasil: Proporsi isolat yang sensitif sulbactam-sefoperazon sebanyak 66,7% dan meningkat menjadi 90% terhadap kombinasi antibiotika. Proporsi isolat yang sensitif fosfomisin hanya 10% dan meningkat dua kali lipat terhadap kombinasi antibiotika. Efek sinergis, indifferent dan antagonis masing-masing ditemukan pada 60%, 36.7% dan 3.3% isolat. Dari curva time kill disimpulkan bahwa kematian bakteri yang cepat terjadi dalam 4 jam pertama. Kesimpulan: kombinasi sulbaktam sepoferazon dan fosfomisin pada sebagian besar isolat Pseudomonas aeruginosa bersifat sinergis dan hanya 1 galur yang menunjukkan efek antagonis.
Open Access Macedonian Journal of Medical Sciences, May 19, 2022
BACKGROUND: Infection with Salmonella enterica subspecies serovar Typhi and Paratyphi A, B, and C... more BACKGROUND: Infection with Salmonella enterica subspecies serovar Typhi and Paratyphi A, B, and C causes typhoid and paratyphoid fever. This is a major source of mortality and morbidity, especially in youngsters. Typhoid fever frequently causes non-specific symptoms. Symptoms that appear obstructively are comparable to those seen in other infectious illnesses and have similar clinical manifestations. Complications can be avoided with early detection and treatment. AIM: This study will look at the hematology parameter profile to determine whether there are any hematological differences between patients with high and low antibodies. METHODS: The research was conducted in Bengkulu, Indonesia, in June 2020 until July 2021. Subject collection was done according to inclusion and exclusion criteria. Subjects have given informed consent. The research included a total of 39 subject patients. Demographic data were collected using a questionnaire and 3 mL of blood was taken. Blood was examined for Tubex TF ® and hematological examination. Statistical analysis used independent t-test and performed with SPSS. RESULTS: The research included a total of 39 subject patients. Most were male and most were aged 21-25 years. The most common symptom felt by the subject was gastrointestinal complaints. The analysis was carried out based on the color scale value of the Tubex TF® which was divided into two groups, 4 color scale groups and 6 color scale groups. Hematological parameters of hemoglobin, hematocrit, WBC, platelets, monocytes, lymphocytes, and neutrophils showed no significant difference. However, color scale group 6 showed lower value than 4 color scale group. CONCLUSION: Hematological indicators in typhoid fever were not substantially changed in Tubex TF ®-positive typhoid fever patients. Clinicians can offer the same therapy in instances with typhoid fever, despite having differing positive Tubex TF ® scores.
Asian Spine Journal, 2016
Preliminary experimental study using a rabbit spondylitis model. Purpose: To observe the ossifica... more Preliminary experimental study using a rabbit spondylitis model. Purpose: To observe the ossification in a micro-environment containing live Mycobacterium tuberculosis transplanted with bone marrow stromal cells (BMSCs) in rabbits. Overview of Literature: BMSCs differentiate to osteoblasts and then osteocytes during ossification. Mycobacterium tuberculosis does not affect BMSC growth in vitro. Methods: Six rabbits were divided into two groups of three rabbits. One group was positive for spondylitis tuberculosis by culture, polymerase chain reaction (PCR), and histopathologically. The other group was positive by PCR and histopathologically. Both groups were treated using BMSC transplantation and anti-tuberculosis drugs. After 6 weeks, ossification was evaluated by enumerating the number of osteoblasts, osteocytes, and lesion level of calcium. Results: Mean number of osteoblasts was 207.00±31.00 in the first group and 220.33±73.46 in the second group. Mean number of intra-lesions osteocytes was in the first and second group was 18.33±30.04 and 31.00±26.87, respectively. Mean calcium level in the first group and second group was 2.94%±0.89% and 2.51%±0.13%, respectively. Total ossification score in the first and second group was 31.00 and 25.67, respectively. Conclusions: Mycobacterium tuberculosis provides support for new bone formation by stimulating intra-lesion calcium metabolism. The microscopic environment containing live Mycobacterium tuberculosis enhances ossification.
Journal Of The Indonesian Medical Association
Pendahuluan: Pseudomonas aeroginosa dapat merupakan penyebab infeksi diberbagai organ. Pilihan an... more Pendahuluan: Pseudomonas aeroginosa dapat merupakan penyebab infeksi diberbagai organ. Pilihan antibiotika tunggal untuk itu menjadi lebih sedikit.Tujuan: Menilai daya antibakteri kombinasi fosfomisin dan sulbaktkam-sefoperazon in vitro terhadap isolat klinis Pseudomonas aeruginosa.Metode: Uji kerentanan 30 isolat klinis Pseudomonas aeruginosa terhadap fosfomisin dan sefoperazone-sulbactam sebagai obat tunggal dan kombinasinya dilakukan pada media cair. Daya antibakteri kombinasi fosmisin dan sulbactam sefoperazon ditentukan dengan menghitung Fraction Inhibition Index (FIC). Time-kill curve dinilai pasca paparan bakteri terhadap antibiotika selama waktu tertentu. Hasil: Proporsi isolat yang sensitif sulbactam-sefoperazon sebanyak 66,7% dan meningkat menjadi 90% terhadap kombinasi antibiotika. Proporsi isolat yang sensitif fosfomisin hanya 10% dan meningkat dua kali lipat terhadap kombinasi antibiotika. Efek sinergis, indifferent dan antagonis masing-masing ditemukan pada 60%, 36.7% ...
Journal Of The Indonesian Medical Association, 2021
Introduction: There is no susceptibility data of E. coli and K. aeromobilis in Indonesia, even da... more Introduction: There is no susceptibility data of E. coli and K. aeromobilis in Indonesia, even data regarding minimal inhibitory concentration (MIC)-based susceptibility of E. coli and K. aeromobilis towards single antibiotic or combination of fosfomycin (FOS) and sulbactam-cepoferazone (SUL-CPZ) is very scarce, even though the data is required by clinicians. Methods: A descriptive observational study was carried out at the Microbiology Clinical Laboratory of the Faculty of Medicine, Universitas Indonesia. Thirty strains each of clinical isolates of E. coli and K. aeromobilis were subjected to MIC determination against FOS and SUL-CPZ. For susceptibility criteria, we adopted the Eucast guideline. The synergism of the combined antibiotics was determined by checkerboard titration. One strain of E. coli and K. aeromobilis showing a synergistic and independent effect against the combined antibiotics was subjected to a time-kill assay. The post-antibiotic effect (PAE) was determined on a...
Archives of Clinical Microbiology, 2015
Background: To design a new scoring model to diagnose dengue in the early phase of illness that c... more Background: To design a new scoring model to diagnose dengue in the early phase of illness that could be used in primary health care facilities. Methods and Findings: Cohort design with consecutive sampling of eighty four participants with one/more clinical features similar to dengue illness within 72 hours after onset of fever. Rapid tests of IgM and NS-1 antigen, and RT-PCR were used to confirm dengue infection. Dengue scoring model with sensitivity and specificity for each value was developed using multivariate logistic regression analysis. Performance of the model was assessed using the ROC curve, and the validity was compared to 1997, 2009 and 2011 WHO dengue classification. Presumptive scoring model used days of fever, presence of myalgia, tourniquet test, total WBC count, monocyte count, and platelet count variables, while probable scoring model used monocytes count and NS-1 antigen for laboratory variables. Patients were most likely to have presumptive dengue illness if they...
Microbiology Indonesia, 2019
Dengue is a mosquito borne virus that spreads rapidly in the world. At present, it is estimated t... more Dengue is a mosquito borne virus that spreads rapidly in the world. At present, it is estimated that more than 3.9 billion people are at risk of being infected with dengue virus (DENV) and there are 96 million clinical cases that have been reported annually in 128 countries worldwide. In DENV infected patients often associated with liver dysfunction which hepatocyte and kuppfer cells as the main target of viral infections. DENV infection induced the expression of several chemokines, which might play an important role during the inflammatory response and pathogenesis of a disease. CXCL10 is known as a chemokine that activated lymphocytes for innate and adaptive immunity, induces tissue damage, and modulates tumor formation. Therefore, we conducted an in vitro study using Huh 7it-1 cells co-cultured with Peripheral Blood Mononuclear Cells (PBMCs) to investigate CXCL10 chemokine induction during DENV infection. Huh 7it-1 cells were grown on 96 micro well plate until a monolayer was formed. The cells were infected with DENV-2 MOI 0.5 FFU/cell and 1 FFU/cell in the presence of PBMCs. Huh7 cell medium were used as negative control. After 2 hours of infection, cells were co-cultured with PBMCs and incubated at 37 ºC with 5% CO for 48 hours. Cell supernatant was collected and CXCL10 chemokine 2 levels were measured using CXCL10 Quantikine ELISA Kit. Statistical analysis was performed by SPSS 23. In the presence of PBMCs, CXCL10 levels from Huh 7it-1 infected by DENV-2 MOI 0,5 FFU/cell and 1 FFU/cell-1-1 were 552,653 ± 22,779 pg mL and 576,787 ± 16,901 pg mL , those levels were higher than negative control. As conclusion, DENV-2 infected Huh 7it-1 cells were able to induce the secretion of CXCL10 from PBMC.
Asian Spine Journal, 2016
Preliminary experimental study using a rabbit spondylitis model. Purpose: To observe the ossifica... more Preliminary experimental study using a rabbit spondylitis model. Purpose: To observe the ossification in a micro-environment containing live Mycobacterium tuberculosis transplanted with bone marrow stromal cells (BMSCs) in rabbits. Overview of Literature: BMSCs differentiate to osteoblasts and then osteocytes during ossification. Mycobacterium tuberculosis does not affect BMSC growth in vitro. Methods: Six rabbits were divided into two groups of three rabbits. One group was positive for spondylitis tuberculosis by culture, polymerase chain reaction (PCR), and histopathologically. The other group was positive by PCR and histopathologically. Both groups were treated using BMSC transplantation and anti-tuberculosis drugs. After 6 weeks, ossification was evaluated by enumerating the number of osteoblasts, osteocytes, and lesion level of calcium. Results: Mean number of osteoblasts was 207.00±31.00 in the first group and 220.33±73.46 in the second group. Mean number of intra-lesions osteocytes was in the first and second group was 18.33±30.04 and 31.00±26.87, respectively. Mean calcium level in the first group and second group was 2.94%±0.89% and 2.51%±0.13%, respectively. Total ossification score in the first and second group was 31.00 and 25.67, respectively. Conclusions: Mycobacterium tuberculosis provides support for new bone formation by stimulating intra-lesion calcium metabolism. The microscopic environment containing live Mycobacterium tuberculosis enhances ossification.
Jakarta: Binarupa Aksara, 1994
xiii, 429 hl
Open Access Macedonian Journal of Medical Sciences
BACKGROUND: Infection with Salmonella enterica subspecies serovar Typhi and Paratyphi A, B, and C... more BACKGROUND: Infection with Salmonella enterica subspecies serovar Typhi and Paratyphi A, B, and C causes typhoid and paratyphoid fever. This is a major source of mortality and morbidity, especially in youngsters. Typhoid fever frequently causes non-specific symptoms. Symptoms that appear obstructively are comparable to those seen in other infectious illnesses and have similar clinical manifestations. Complications can be avoided with early detection and treatment. AIM: This study will look at the hematology parameter profile to determine whether there are any hematological differences between patients with high and low antibodies. METHODS: The research was conducted in Bengkulu, Indonesia, in June 2020 until July 2021. Subject collection was done according to inclusion and exclusion criteria. Subjects have given informed consent. The research included a total of 39 subject patients. Demographic data were collected using a questionnaire and 3 mL of blood was taken. Blood was examined...
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Papers by agus syahrurachman