Papers by afshan chaudhry
Pediatric Research, Oct 29, 2021
The Editor-in-Chief is publishing an editorial Expression of Concern for this article. In 2019 a ... more The Editor-in-Chief is publishing an editorial Expression of Concern for this article. In 2019 a Correction 1 was published explaining that this study was part of a larger project. 2 However, concerns about the reliability of the data have now been raised. 3 These concerns are that statistical analysis of the summary data in this article and in ref. 2 that appear to be identical with those reported in ref. 4 suggests that these studies were not conducted independently from the study reported in ref. 4 The original data from this study are not available for examination. Readers are therefore advised to interpret the results and conclusions presented in this article with caution. Afshan A. Chaudhry has not explicitly stated whether they agree with this notice.
Pediatric Research, Nov 29, 2019
Pediatric Research, 2021
The Editor-in-Chief is publishing an editorial Expression of Concern for this article. In 2019 a ... more The Editor-in-Chief is publishing an editorial Expression of Concern for this article. In 2019 a Correction 1 was published explaining that this study was part of a larger project. 2 However, concerns about the reliability of the data have now been raised. 3 These concerns are that statistical analysis of the summary data in this article and in ref. 2 that appear to be identical with those reported in ref. 4 suggests that these studies were not conducted independently from the study reported in ref. 4 The original data from this study are not available for examination. Readers are therefore advised to interpret the results and conclusions presented in this article with caution. Afshan A. Chaudhry has not explicitly stated whether they agree with this notice.
Pediatric Research, 2009
Growth hormone (GH) deficiency in children causes severe growth retardation, vitamin D deficiency... more Growth hormone (GH) deficiency in children causes severe growth retardation, vitamin D deficiency, and osteopenia. We investigated whether alfacalcidol (1OHD) alone or in combination with GH can improve bone formation. Forty hypophysectomized female rats (HX) at the age of 8 wk were divided into HX, HX ϩ 1OHD (oral 0.25 g/kg daily), HXϩGH (0.666 mg/0.2 mL SC daily) and HXϩGH ϩ 1OHD groups for a 4-wk study. Results showed that GH increased body weight, bone area, bone mineral content (BMC), and bone mineral density (BMD), whereas 1OHD only increased BMC and BMD. In cortical bone, GH increased both periosteal and endocortical bone formation resulting in a significant increase in cortical size and area in percentage, whereas 1OHD suppressed endocortical erosion surface per bone surface (ES/BS) without a significant effect on bone formation rate per bone surface (BFR/ BS). In trabecular bone, GH mitigated the bone loss by increasing BFR/BS, whereas the 1OHD effect was by suppression of trabecular bone turnover in the HX rats. The combination of GH and 1OHD had no additive effect on increasing trabecular bone mass. In conclusion, GH activates new bone formation and increases bone turnover whereas 1OHD suppresses bone turnover. The combination intervention does not seem to provide any additive benefit. (Pediatr Res 65: 403-408, 2009)
Metabolism, 1991
The subcellular distribution of G, (the a-subunit of guanine nucleotide-binding stimulatory prote... more The subcellular distribution of G, (the a-subunit of guanine nucleotide-binding stimulatory protein of adenylyl cyclase) was examined in interscapular brown adipose tissue (IBAT) to determine (1) if G, is completely colocalized with adenylyl cyclase in the plasma membrane, and (2) whether cold exposure, which increases adenylyl cyclase activity, changes the subcellular distribution of G,. Subcellular fractions were prepared from IBAT by differential centrifugation and analyzed for G,, by immunoblotting. Adenylyl cyclase activity and G, were detected in all the subcellular fractions except the cytosol. The plasma membrane fraction showed the greatest enrichment of adenylyl cyclase and G,. However, the enrichment of adenylyl cyclase in the plasma membrane fraction was greater than that for G, which was also associated to a large degree with the mitochondrial fraction. Thus, compared with the mitochondrial fraction, both B'nucleotidase and adenylyl cyclase were enriched by over 200% in the plasma membrane fraction, but G, was enriched by only 50%. Exposure of rats to 4°C for 3 days increased fluoride-stimulated adenylyl cyclase activity, but did not increase the amount of immunoreactive G, in any of the subcellular fractions examined. The above results demonstrate that not all G, in IBAT is colocalized with adenylyl cyclase in the plasma membrane. The finding that cold exposure did not change the subcellular distribution of G, indicates that the cold-induced increase in adenvlvl cvclase activitv is not due to translocation of G-from subcellular compartments to the plasma membrane.
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Papers by afshan chaudhry