... Daha önce yapılan meme biyopsilerinde sellüler atipi, atipik duktal hiperplazi, lobüler neopl... more ... Daha önce yapılan meme biyopsilerinde sellüler atipi, atipik duktal hiperplazi, lobüler neoplazi, juvenil papillomatozis saptanan kadınlarda meme kanseri riski artmıştır. ... Mutasyona uğramış BRCA1 ve BRCA2 genlerini taşıyanlarda meme kanseri riski belirgin olarak artmıştır. ...
Amaç. Embriyonel hayatta tamamlanan kişiye özel dermatoglifik örnek dağılımlarının farklılığından... more Amaç. Embriyonel hayatta tamamlanan kişiye özel dermatoglifik örnek dağılımlarının farklılığından yola çıkılarak çalışmamızdaki Ege Bölgesi erkek elit sporcularının (ES) dermatoglifik örneklerinin, erkek sedanter bireylerden (SB) farkının olup olmadığının araştırılmasını ve her iki grubun antropometrik ve biyokimyasal verilerinin değerlendirilmesini amaçladık. Yöntem. Ege Bölgesindeki erkek ES'lerin (n=105) ve erkek SB'in (n=105) dermatoglifik örnek çalışmasını, el antropometrik ölçümlerini ve biyokimyasal testlerini gerçekleştirdik. Genetikte yardımcı tanı yöntemlerinden olan dermatoglifik çalışmasıyla, erkek ES'ların ve erkek kontrol grubunun dermal örneklerini inceleyerek, erkek ES'ların, erkek kontrol grubundan farklı örnek dağılımına sahip olup olmadıklarını araştırdık. Bulgular. Erkek ES'ların ve SB'lerin parmak ucu dermal örnekleri incelenerek, erkek ES'lerin erkek SB'lerden farklı örnek dağılımına sahip oldukları saptandı. Antropometrik ölçüml...
Obezite, tüm ırk veya etnik gruplarda ve her yaşta görülen toplumsal bir sağlık proble-midir. Gel... more Obezite, tüm ırk veya etnik gruplarda ve her yaşta görülen toplumsal bir sağlık proble-midir. Gelişmekte olan diğer ülkelerde olduğu gibi, Türkiye’de de obezite; kentleşme, aile geliri, eğitim ve diğer yüksek sosyoekonomik durum belirteçleri ile yakından ilişkili bir olgudur. Bu çalışmanın amacı, uluslar arası tanımları kullanarak Muğla’da 6-15 yaş grubu okul çocuklarındaki fazla kilo ve obezite prevalanslarını belirlemek ve diğer ülke çalışmaları ile karşılaştırmaktır. Bu çalışma, Muğla yöresinde yapılan ilk obezite araştırmasıdır.
Purpose: In this study, the relationship between sex hormone levels and erectile dysfunction (ED)... more Purpose: In this study, the relationship between sex hormone levels and erectile dysfunction (ED), as well as the necessity of routinely measuring sex hormone levels were evaluated. Materials and Methods: This study included one hundred patients admitted to a urology clinic for sexual dysfunction. To determine the hormone levels, following the history (included IIEF-5 score) and physical examination, triple blood samples were collected at intervals of 15 minutes between 08:00 and 10:00 am. Total and free testosterone, prolactin, follicle stimulating hormone and luteinizing hormone levels were studied. Results: Mean age was 43 (23- 80) years. IIEF-5 score was less than 21 [9.8- 4.3 (3- 19)] in all study groups. There was a statistically significant correlation between tT and FSH, as well as between LH and FSH in Pearson (r =- 0.513, p < 0.001, respectively) and also in Spearman tests (r =- 0.224, p = 0.042 and r =- 0.459, p < 0.001, respectively). However, there was no correlat...
Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix ... more Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in post-menopausal women. Method: Body weight, percent body fat, body mass index, estimated maximal oxygen con-sumption, blood lipids, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 concentrations were assessed and their matrix metalloproteinase-9/tissue inhibitor of me-talloproteinase-1 molar ratios were calculated in exercise (n=12) and control (n=12) groups. Exercise group completed an eight-week walking program at moderate (~6.29±0.15km/h; ~62 % maximum heart rate reserve) intensity. Results: Significant changes in estimated maximal oxygen consumption, systolic and dia-stolic blood pressures, body weight, and body mass index (p<.05) were determined in exer-cise group. However, there were no significant changes in the measured blood lipids (triglyc-eride, cholesterol, high-density lipoprotein chole...
Aim: The severity and extent of coronary artery disease can be examined by the Gensini score. The... more Aim: The severity and extent of coronary artery disease can be examined by the Gensini score. The purpose of the present study is to investigate the association of Adiponectin, IGF-1, IGFBP-3 and the number of vessels with stenosis, the Gensini scoring and the usability of the parameters as early markers. Methods: All patients were collected from the cardiology outpatient clinic with Coronary Artery Disease symptoms. Coronary angiography was planned for all 158 symptomatic patients with Coronary Artery Disease. All patients had coronary anjiograpy and Gensini scoring for the assessment of severity of atherosclerosis. Peripheral venous blood IGF-1, IGFBP-3, Adiponectin levels were assessed from all patients and the control group before the angiography. Results: Gensini score of the control group, Group 1, Group 2 and Group 3 were 0.0±0.0, 10.29±10.79, 23.92±21.79 and 56.51±35.39 respectively. The mean Adiponectin levels were statistically different among the groups (p=0.013) and the ...
Obesity can be induced by a high fat diet. Dehydroepiandrosterone sulfate decreases body fat mass... more Obesity can be induced by a high fat diet. Dehydroepiandrosterone sulfate decreases body fat mass and have an antiatherogenic effect. The objective of our study was to investigate the high fat diet changes and the effect of dehydroepiandrosterone sulfate on the antioxidant status, zinc and copper levels in the rat hind leg muscle tissue. Thirty-seven female rats were divided into 4 groups. Group 1 (control, n=11) were fed with standard rat chow, Group 2, 3 and 4 with high fat diet (65 %) for five months. Dehydroepiandrosterone sulfate was administered as 1 mg/kg for group 3 (n=9) and as 10 mg/kg for group 4 (n=8) daily for seven days. The same amount of saline was injected to group 1 and 2 (n=9). After decapitation, right hind leg of the rat was collected and kept frozen. Muscle tissues were used for the determination of nitrotyrosine, myeloperoxidase activity, copper and zinc levels. As a conclusion, dehydroepiandrosterone sulfate significantly decreases the oxidant status paramete...
In general, histone ADMA is associated with active transcription, whereas histone SDMA is correla... more In general, histone ADMA is associated with active transcription, whereas histone SDMA is correlated with transcriptional repression (10). While histones are true PRMT substrates, the majority of nuclear arginine methylation is found in heterogeneous nuclear ribonucleoproteins, implying that PRMTs can also regulate gene expression post-transcriptionally (11).
Kayıt tarihi: 30 Mayıs 2008, Kabul tarihi: 20 Ekim 2008 ABSTRACT Purpose: To examine the effects ... more Kayıt tarihi: 30 Mayıs 2008, Kabul tarihi: 20 Ekim 2008 ABSTRACT Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in post-menopausal women. Method: Body weight, percent body fat, body mass index, estimated maximal oxygen consumption, blood lipids, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 concentrations were assessed and their matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1 molar ratios were calculated in exercise (n=12) and control (n=12) groups. Exercise group completed an eight-week walking program at moderate (~6.29±0.15km/h; ~62% maximum heart rate reserve) intensity. Results: Significant changes in estimated maximal oxygen consumption, systolic and diastolic blood pressures, body weight, and body mass index (p< .05) were determined in exercise group. However, there were no significant changes in the measured blood lipid...
Migration, invasion, metastasis and angiogenesis associated with cancer depend on the surrounding... more Migration, invasion, metastasis and angiogenesis associated with cancer depend on the surrounding microenvironment. Angiogenesis, the growth of new capillaries, is a regulator of cancer growth and a useful target for cancer therapy. We examined matrix protein interactions in a gastric cancer cell culture that was treated with different doses of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE). We also investigated the relations among the levels of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), endostatin (ES) and trombospondin-1 (TSP-1). Cytotoxity of CAPE was measured using the 3-(4,5-dmethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. We examined the behavior of cells on laminin and collagen I coated surfaces in response to the angiogenic effect of these matrix molecules. We examined the protein alterations of these matrix molecules immunohistochemically and measured the levels of VEGF, MMP-9, ES and TSP-1 using the ELISA test. We showed that application of CAPE to the gastric cancer cell line on tissue culture plastic, laminin and collagen I significantly decreased the VEGF and MMP-9 protein levels. We found that TSP-1 levels were increased significantly in the gastric cancer cells after application of CAPE. The protein levels of gastric cancer cells also were increased significantly when tissue was cultured on laminin and collagen I. Application of CAPE to cells on laminin or collagen I coated surfaces significantly increased all of the proteins except ES. ES levels were increased on the collagen I covered surfaces, but the laminin surface decreased the levels of ES significantly. We demonstrated the beneficial effect of CAPE on a gastric cancer cell line including inhibition of proliferation and induction of some proteins that might be related to decreased angiogenesis.
Purpose: We investigate the underlying pathophysiological cause of primary nocturnal enuresis by ... more Purpose: We investigate the underlying pathophysiological cause of primary nocturnal enuresis by comparing electrolyte alterations in urine samples of enuretics during the daytime and nighttime compared with those of nonenuretic subjects. Materials and Methods: Urine output, urine specific gravity and urinary electrolytes in 15 enuretic and 12 nonenuretic children were measured. We collected daytime serum and urine samples of children fed a similar diet between 7 a.m. and 7 p.m., and nighttime between samples 7 p.m. and 7 a.m. Urinary calciudcreatinine ratio, tubular reabsorption of phosphorus and excretions of fractional sodium and potassium were calculated. Results: There was no significant difference between the calciudcreatinine ratio ratios. There was a significant increase in fractional sodium and fractional potassium values in enuretics compared to nonenuretics during the day and at night. Daytime and nighttime fractional sodium and fractional potassium values in enuretics were similar. In contrast to nonenuretics, enuretic patients had no diurnal variation of fractional sodium. There was significant positive correlation between bedwetting status, and fractional sodium and fractional potassium. Conclusions: Since sodium and potassium excretions were higher in enuretic patients than nonenuretic children, and no significant diurnal variation in urinary excretion of these ions there might be a difference in the mechanism of reabsorption of sodium and potassium between enuretic and nonenuretic children.
Gastric cancer has a high incidence and is one of the leading causes of mortality both in males a... more Gastric cancer has a high incidence and is one of the leading causes of mortality both in males and females. Angiogenesis is the formation of new capillaries from existing blood vessels through which oxygen and nutrients are transported to the tissues to promote growth, and it plays a pivotal role in the invasion and metastasis of cancer caused by oncogenes. Even though this process is useful in tissue repair and normal growth, it also facilitates the growth of cancer tissues and their metastasis via the hematogenous route, and in this way, it speeds up metastasis and invasion. In the present study, we aimed to investigate the relationship between angiogenesis and markers such as VEGF, MMP, TSP-1 and Endostatin in a CAPE-administered gastric cancer cell in culture. Following CAPE administration, cytotoxicity was measured by MTT test. Angiogenic behavior of cells was examined immunohistochemically by VEGF, MMP and Endostatin, and TSP – 1 level was measured by RT-PCR test. MTT test sh...
This is an Open Access article distributed under the terms of the Creative Commons Attribution No... more This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original ...
The lipid profile is known to alter in patients with infection, but there has not been a study of... more The lipid profile is known to alter in patients with infection, but there has not been a study of the apolipoprotein levels in serum of otherwise healthy children during infection. Lipids, lipoproteins, apolipoproteins A-1 and B and lipoprotein (a) were evaluated prospectively in 31 consecutive children, aged 4-15 years, who were admitted to the hospital with bacterial pharyngitis. The degree of dyslipidemia associated with bacterial pharyngitis was assessed using each child as hisher own control and by comparison with 79 healthy children who had not had an infection during the past 3 months. Serum total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, apolipoprotein A-1 and apolipoprotein B levels were significantly decreased during the symptomatic phase of the disease, whereas the serum triglyceride level was slightly elevated. Serum lipoprotein (a) concentration did not change significantly. In conclusion, it is suggested that serum lipids, lipoproteins and apolipoproteins should not be assessed during infection because of the possible transient changes of these parameters during infection or inflammation.
The purpose of the study was to determine differences and correlations between the blood glucose ... more The purpose of the study was to determine differences and correlations between the blood glucose values of venous blood and the first and second drops of capillary blood samples taken in three different ways. Blood samples were (a) venous blood, (b) the first and second drops of capillary blood from the middle finger of the right hand (only washed with soap and water), and (c) the first and second drops of capillary blood from the middle finger of the left hand (washed with soap and water and cleaned with alcohol). It was concluded that the fasting capillary blood glucose values could be used in place of venous blood glucose values, that only washing the hands with neutral soap and water for 30 s could be sufficient for capillary blood glucose measurement, and that the first or second blood drop from a clean hand could be used for capillary blood glucose measurement.
Öz Amaç: Kas invaziv olmayan mesane kanserinde (KİOMK) tedavinin anjiyogenik [vasküler endotelyal... more Öz Amaç: Kas invaziv olmayan mesane kanserinde (KİOMK) tedavinin anjiyogenik [vasküler endotelyal büyüme faktörü-2 (VEGF-2) ve matriks metaloproteinaz-2 (MMP-2)] ve antianjiyogenik [endostatin (ES) and trombospondin-1 (TSP-1)] faktörler üzerine etkilerini araştırmayı amaçladık. Gereç ve Yöntem: KİOMK'li 30 hasta ve aynı yaş grubundaki 30 kontrol çalışmaya dahil edildi. Hastaların kan örnekleri üç defa alınarak yukarıda bahsedilen markerlar enzime bağlı immünosorbent testi yöntemiyle ölçüldü. İlk kan örneği transüretral rezeksiyon-mesane tümörü (TUR-MT) öncesi,
International Journal of Neuroscience, Jun 1, 2004
The aim of the study was to investigate the effect of regular exercise on maximum oxygen uptake c... more The aim of the study was to investigate the effect of regular exercise on maximum oxygen uptake capacity (VO2max), reaction time (RT), testosterone (T), growth hormone (GH), insulin-like growth factor-I (IGF-I) in athletes compared to sedentary controls. VO2max, RT, T, GH, and IGF-I levels were 31.2 +/- 6.2 ml/min/kg, 106.7 +/- 23.2 s, 8.3 +/- 1.3 ng/mL, 1.6 +/- 0.7 ng/mL, 106.5 +/- 27.0 ng/mL in master athlete group and 18.8 +/- 5.1 ml/min/kg, 148.3 +/- 39.3 s, 5.4 +/- 1.7 ng/mL, 0.8 +/- 0.3 ng/mL, 90.2 +/- 23.8 ng/mL in sedentary control group, respectively. The differences between regularly exercising males and the control group of sedentary males were found to be statistically significant. The results showed that long-term exercise decreased RT and increased VO2max, T, and GH in elderly males; elevated serum T and GH levels may be advantageous for brain functions.
ABSTRACT Background: Dehydroepiandrosterone sulphate decreases body fat mass and have a known ant... more ABSTRACT Background: Dehydroepiandrosterone sulphate decreases body fat mass and have a known antiatherogenic effect. The aim of our study was to investigate the high fat diet changes and the effect of dehydroepiandrosterone sulphate on the nitrite/nitrate system, myeloperoxidase activity and malondialdehyde levels in the rat cerebrum tissue. Material and Methods: Thirty-eight female rats were classified into four groups. Group I (control) (n=11) were fed with standard rat chow; Group IIa, IIb, and IIc with high fat diet for five months. Dehydroepiandrosterone sulphate was administered intraperitoneal as 1 mg/kg for group IIb (n=9) and as 10 mg/kg for group IV (n=9) for seven days. Group I and IIa received the same amount of SF fluid instead. After decapitation, brain tissues were analyzed for malondialdehyde, nitrite/ nitrate, trace element levels and myeloperoxidase activity. Results: Nitrite/nitrate levels were statistically higher in Group IIc than Group I (p&lt;0.001). Malondialdehyde levels were lower in the high fat diet group (p=0.037). Nitrite/nitrate levels were higher and malondialdehyde was significantly lower in group IIc than in group I (p=0.000 and p=0.037, respectively). Conclusions: Dehydroepiandrosterone sulphate in 10 mg/kg dose significantly increases NOx levels and decreases malondialdehyde levels in the cerebral tissues of the rats fed with high fat diet. These findings suggest that the antiatherogenic effect of dehydroepiandrosterone sulphate could be associated with the decrease of the malondialdehyde levels.
Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix ... more Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in post-menopausal women. Method: Body weight, percent body fat, body mass index, estimated maximal oxygen consumption, blood lipids, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 concentrations were assessed and their matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1 molar ratios were calculated in exercise (n=12) and control (n=12) groups. Exercise group completed an eight-week walking program at moderate (~6.29±0.15km/h; ~62% maximum heart rate reserve) intensity. Results: Significant changes in estimated maximal oxygen consumption, systolic and diastolic blood pressures, body weight, and body mass index (p< .05) were determined in exercise group. However, there were no significant changes in the measured blood lipids (triglyceride, cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol), matrix metalloproteinase-9, and tissue inhibitor of metalloproteinase-1 concentrations, and matrix metalloproteinase-9/ tissue inhibitor of metalloproteinase-1 molar ratio of exercise group, except for a nearly significant change in tissue inhibitor of metalloproteinase-1 level (p= .092). We determined no significant changes in any of the measured parameters in control group. Conclusion: Despite its protective effects against coronary heart disease risks, the duration and intensity of this program is not sufficient to cause significant changes in blood lipids, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinase-1 concentrations. A higher-intensity, longer-duration program accompanied with diet is proposed.
... Daha önce yapılan meme biyopsilerinde sellüler atipi, atipik duktal hiperplazi, lobüler neopl... more ... Daha önce yapılan meme biyopsilerinde sellüler atipi, atipik duktal hiperplazi, lobüler neoplazi, juvenil papillomatozis saptanan kadınlarda meme kanseri riski artmıştır. ... Mutasyona uğramış BRCA1 ve BRCA2 genlerini taşıyanlarda meme kanseri riski belirgin olarak artmıştır. ...
Amaç. Embriyonel hayatta tamamlanan kişiye özel dermatoglifik örnek dağılımlarının farklılığından... more Amaç. Embriyonel hayatta tamamlanan kişiye özel dermatoglifik örnek dağılımlarının farklılığından yola çıkılarak çalışmamızdaki Ege Bölgesi erkek elit sporcularının (ES) dermatoglifik örneklerinin, erkek sedanter bireylerden (SB) farkının olup olmadığının araştırılmasını ve her iki grubun antropometrik ve biyokimyasal verilerinin değerlendirilmesini amaçladık. Yöntem. Ege Bölgesindeki erkek ES'lerin (n=105) ve erkek SB'in (n=105) dermatoglifik örnek çalışmasını, el antropometrik ölçümlerini ve biyokimyasal testlerini gerçekleştirdik. Genetikte yardımcı tanı yöntemlerinden olan dermatoglifik çalışmasıyla, erkek ES'ların ve erkek kontrol grubunun dermal örneklerini inceleyerek, erkek ES'ların, erkek kontrol grubundan farklı örnek dağılımına sahip olup olmadıklarını araştırdık. Bulgular. Erkek ES'ların ve SB'lerin parmak ucu dermal örnekleri incelenerek, erkek ES'lerin erkek SB'lerden farklı örnek dağılımına sahip oldukları saptandı. Antropometrik ölçüml...
Obezite, tüm ırk veya etnik gruplarda ve her yaşta görülen toplumsal bir sağlık proble-midir. Gel... more Obezite, tüm ırk veya etnik gruplarda ve her yaşta görülen toplumsal bir sağlık proble-midir. Gelişmekte olan diğer ülkelerde olduğu gibi, Türkiye’de de obezite; kentleşme, aile geliri, eğitim ve diğer yüksek sosyoekonomik durum belirteçleri ile yakından ilişkili bir olgudur. Bu çalışmanın amacı, uluslar arası tanımları kullanarak Muğla’da 6-15 yaş grubu okul çocuklarındaki fazla kilo ve obezite prevalanslarını belirlemek ve diğer ülke çalışmaları ile karşılaştırmaktır. Bu çalışma, Muğla yöresinde yapılan ilk obezite araştırmasıdır.
Purpose: In this study, the relationship between sex hormone levels and erectile dysfunction (ED)... more Purpose: In this study, the relationship between sex hormone levels and erectile dysfunction (ED), as well as the necessity of routinely measuring sex hormone levels were evaluated. Materials and Methods: This study included one hundred patients admitted to a urology clinic for sexual dysfunction. To determine the hormone levels, following the history (included IIEF-5 score) and physical examination, triple blood samples were collected at intervals of 15 minutes between 08:00 and 10:00 am. Total and free testosterone, prolactin, follicle stimulating hormone and luteinizing hormone levels were studied. Results: Mean age was 43 (23- 80) years. IIEF-5 score was less than 21 [9.8- 4.3 (3- 19)] in all study groups. There was a statistically significant correlation between tT and FSH, as well as between LH and FSH in Pearson (r =- 0.513, p < 0.001, respectively) and also in Spearman tests (r =- 0.224, p = 0.042 and r =- 0.459, p < 0.001, respectively). However, there was no correlat...
Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix ... more Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in post-menopausal women. Method: Body weight, percent body fat, body mass index, estimated maximal oxygen con-sumption, blood lipids, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 concentrations were assessed and their matrix metalloproteinase-9/tissue inhibitor of me-talloproteinase-1 molar ratios were calculated in exercise (n=12) and control (n=12) groups. Exercise group completed an eight-week walking program at moderate (~6.29±0.15km/h; ~62 % maximum heart rate reserve) intensity. Results: Significant changes in estimated maximal oxygen consumption, systolic and dia-stolic blood pressures, body weight, and body mass index (p<.05) were determined in exer-cise group. However, there were no significant changes in the measured blood lipids (triglyc-eride, cholesterol, high-density lipoprotein chole...
Aim: The severity and extent of coronary artery disease can be examined by the Gensini score. The... more Aim: The severity and extent of coronary artery disease can be examined by the Gensini score. The purpose of the present study is to investigate the association of Adiponectin, IGF-1, IGFBP-3 and the number of vessels with stenosis, the Gensini scoring and the usability of the parameters as early markers. Methods: All patients were collected from the cardiology outpatient clinic with Coronary Artery Disease symptoms. Coronary angiography was planned for all 158 symptomatic patients with Coronary Artery Disease. All patients had coronary anjiograpy and Gensini scoring for the assessment of severity of atherosclerosis. Peripheral venous blood IGF-1, IGFBP-3, Adiponectin levels were assessed from all patients and the control group before the angiography. Results: Gensini score of the control group, Group 1, Group 2 and Group 3 were 0.0±0.0, 10.29±10.79, 23.92±21.79 and 56.51±35.39 respectively. The mean Adiponectin levels were statistically different among the groups (p=0.013) and the ...
Obesity can be induced by a high fat diet. Dehydroepiandrosterone sulfate decreases body fat mass... more Obesity can be induced by a high fat diet. Dehydroepiandrosterone sulfate decreases body fat mass and have an antiatherogenic effect. The objective of our study was to investigate the high fat diet changes and the effect of dehydroepiandrosterone sulfate on the antioxidant status, zinc and copper levels in the rat hind leg muscle tissue. Thirty-seven female rats were divided into 4 groups. Group 1 (control, n=11) were fed with standard rat chow, Group 2, 3 and 4 with high fat diet (65 %) for five months. Dehydroepiandrosterone sulfate was administered as 1 mg/kg for group 3 (n=9) and as 10 mg/kg for group 4 (n=8) daily for seven days. The same amount of saline was injected to group 1 and 2 (n=9). After decapitation, right hind leg of the rat was collected and kept frozen. Muscle tissues were used for the determination of nitrotyrosine, myeloperoxidase activity, copper and zinc levels. As a conclusion, dehydroepiandrosterone sulfate significantly decreases the oxidant status paramete...
In general, histone ADMA is associated with active transcription, whereas histone SDMA is correla... more In general, histone ADMA is associated with active transcription, whereas histone SDMA is correlated with transcriptional repression (10). While histones are true PRMT substrates, the majority of nuclear arginine methylation is found in heterogeneous nuclear ribonucleoproteins, implying that PRMTs can also regulate gene expression post-transcriptionally (11).
Kayıt tarihi: 30 Mayıs 2008, Kabul tarihi: 20 Ekim 2008 ABSTRACT Purpose: To examine the effects ... more Kayıt tarihi: 30 Mayıs 2008, Kabul tarihi: 20 Ekim 2008 ABSTRACT Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in post-menopausal women. Method: Body weight, percent body fat, body mass index, estimated maximal oxygen consumption, blood lipids, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 concentrations were assessed and their matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1 molar ratios were calculated in exercise (n=12) and control (n=12) groups. Exercise group completed an eight-week walking program at moderate (~6.29±0.15km/h; ~62% maximum heart rate reserve) intensity. Results: Significant changes in estimated maximal oxygen consumption, systolic and diastolic blood pressures, body weight, and body mass index (p< .05) were determined in exercise group. However, there were no significant changes in the measured blood lipid...
Migration, invasion, metastasis and angiogenesis associated with cancer depend on the surrounding... more Migration, invasion, metastasis and angiogenesis associated with cancer depend on the surrounding microenvironment. Angiogenesis, the growth of new capillaries, is a regulator of cancer growth and a useful target for cancer therapy. We examined matrix protein interactions in a gastric cancer cell culture that was treated with different doses of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE). We also investigated the relations among the levels of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), endostatin (ES) and trombospondin-1 (TSP-1). Cytotoxity of CAPE was measured using the 3-(4,5-dmethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. We examined the behavior of cells on laminin and collagen I coated surfaces in response to the angiogenic effect of these matrix molecules. We examined the protein alterations of these matrix molecules immunohistochemically and measured the levels of VEGF, MMP-9, ES and TSP-1 using the ELISA test. We showed that application of CAPE to the gastric cancer cell line on tissue culture plastic, laminin and collagen I significantly decreased the VEGF and MMP-9 protein levels. We found that TSP-1 levels were increased significantly in the gastric cancer cells after application of CAPE. The protein levels of gastric cancer cells also were increased significantly when tissue was cultured on laminin and collagen I. Application of CAPE to cells on laminin or collagen I coated surfaces significantly increased all of the proteins except ES. ES levels were increased on the collagen I covered surfaces, but the laminin surface decreased the levels of ES significantly. We demonstrated the beneficial effect of CAPE on a gastric cancer cell line including inhibition of proliferation and induction of some proteins that might be related to decreased angiogenesis.
Purpose: We investigate the underlying pathophysiological cause of primary nocturnal enuresis by ... more Purpose: We investigate the underlying pathophysiological cause of primary nocturnal enuresis by comparing electrolyte alterations in urine samples of enuretics during the daytime and nighttime compared with those of nonenuretic subjects. Materials and Methods: Urine output, urine specific gravity and urinary electrolytes in 15 enuretic and 12 nonenuretic children were measured. We collected daytime serum and urine samples of children fed a similar diet between 7 a.m. and 7 p.m., and nighttime between samples 7 p.m. and 7 a.m. Urinary calciudcreatinine ratio, tubular reabsorption of phosphorus and excretions of fractional sodium and potassium were calculated. Results: There was no significant difference between the calciudcreatinine ratio ratios. There was a significant increase in fractional sodium and fractional potassium values in enuretics compared to nonenuretics during the day and at night. Daytime and nighttime fractional sodium and fractional potassium values in enuretics were similar. In contrast to nonenuretics, enuretic patients had no diurnal variation of fractional sodium. There was significant positive correlation between bedwetting status, and fractional sodium and fractional potassium. Conclusions: Since sodium and potassium excretions were higher in enuretic patients than nonenuretic children, and no significant diurnal variation in urinary excretion of these ions there might be a difference in the mechanism of reabsorption of sodium and potassium between enuretic and nonenuretic children.
Gastric cancer has a high incidence and is one of the leading causes of mortality both in males a... more Gastric cancer has a high incidence and is one of the leading causes of mortality both in males and females. Angiogenesis is the formation of new capillaries from existing blood vessels through which oxygen and nutrients are transported to the tissues to promote growth, and it plays a pivotal role in the invasion and metastasis of cancer caused by oncogenes. Even though this process is useful in tissue repair and normal growth, it also facilitates the growth of cancer tissues and their metastasis via the hematogenous route, and in this way, it speeds up metastasis and invasion. In the present study, we aimed to investigate the relationship between angiogenesis and markers such as VEGF, MMP, TSP-1 and Endostatin in a CAPE-administered gastric cancer cell in culture. Following CAPE administration, cytotoxicity was measured by MTT test. Angiogenic behavior of cells was examined immunohistochemically by VEGF, MMP and Endostatin, and TSP – 1 level was measured by RT-PCR test. MTT test sh...
This is an Open Access article distributed under the terms of the Creative Commons Attribution No... more This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original ...
The lipid profile is known to alter in patients with infection, but there has not been a study of... more The lipid profile is known to alter in patients with infection, but there has not been a study of the apolipoprotein levels in serum of otherwise healthy children during infection. Lipids, lipoproteins, apolipoproteins A-1 and B and lipoprotein (a) were evaluated prospectively in 31 consecutive children, aged 4-15 years, who were admitted to the hospital with bacterial pharyngitis. The degree of dyslipidemia associated with bacterial pharyngitis was assessed using each child as hisher own control and by comparison with 79 healthy children who had not had an infection during the past 3 months. Serum total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, apolipoprotein A-1 and apolipoprotein B levels were significantly decreased during the symptomatic phase of the disease, whereas the serum triglyceride level was slightly elevated. Serum lipoprotein (a) concentration did not change significantly. In conclusion, it is suggested that serum lipids, lipoproteins and apolipoproteins should not be assessed during infection because of the possible transient changes of these parameters during infection or inflammation.
The purpose of the study was to determine differences and correlations between the blood glucose ... more The purpose of the study was to determine differences and correlations between the blood glucose values of venous blood and the first and second drops of capillary blood samples taken in three different ways. Blood samples were (a) venous blood, (b) the first and second drops of capillary blood from the middle finger of the right hand (only washed with soap and water), and (c) the first and second drops of capillary blood from the middle finger of the left hand (washed with soap and water and cleaned with alcohol). It was concluded that the fasting capillary blood glucose values could be used in place of venous blood glucose values, that only washing the hands with neutral soap and water for 30 s could be sufficient for capillary blood glucose measurement, and that the first or second blood drop from a clean hand could be used for capillary blood glucose measurement.
Öz Amaç: Kas invaziv olmayan mesane kanserinde (KİOMK) tedavinin anjiyogenik [vasküler endotelyal... more Öz Amaç: Kas invaziv olmayan mesane kanserinde (KİOMK) tedavinin anjiyogenik [vasküler endotelyal büyüme faktörü-2 (VEGF-2) ve matriks metaloproteinaz-2 (MMP-2)] ve antianjiyogenik [endostatin (ES) and trombospondin-1 (TSP-1)] faktörler üzerine etkilerini araştırmayı amaçladık. Gereç ve Yöntem: KİOMK'li 30 hasta ve aynı yaş grubundaki 30 kontrol çalışmaya dahil edildi. Hastaların kan örnekleri üç defa alınarak yukarıda bahsedilen markerlar enzime bağlı immünosorbent testi yöntemiyle ölçüldü. İlk kan örneği transüretral rezeksiyon-mesane tümörü (TUR-MT) öncesi,
International Journal of Neuroscience, Jun 1, 2004
The aim of the study was to investigate the effect of regular exercise on maximum oxygen uptake c... more The aim of the study was to investigate the effect of regular exercise on maximum oxygen uptake capacity (VO2max), reaction time (RT), testosterone (T), growth hormone (GH), insulin-like growth factor-I (IGF-I) in athletes compared to sedentary controls. VO2max, RT, T, GH, and IGF-I levels were 31.2 +/- 6.2 ml/min/kg, 106.7 +/- 23.2 s, 8.3 +/- 1.3 ng/mL, 1.6 +/- 0.7 ng/mL, 106.5 +/- 27.0 ng/mL in master athlete group and 18.8 +/- 5.1 ml/min/kg, 148.3 +/- 39.3 s, 5.4 +/- 1.7 ng/mL, 0.8 +/- 0.3 ng/mL, 90.2 +/- 23.8 ng/mL in sedentary control group, respectively. The differences between regularly exercising males and the control group of sedentary males were found to be statistically significant. The results showed that long-term exercise decreased RT and increased VO2max, T, and GH in elderly males; elevated serum T and GH levels may be advantageous for brain functions.
ABSTRACT Background: Dehydroepiandrosterone sulphate decreases body fat mass and have a known ant... more ABSTRACT Background: Dehydroepiandrosterone sulphate decreases body fat mass and have a known antiatherogenic effect. The aim of our study was to investigate the high fat diet changes and the effect of dehydroepiandrosterone sulphate on the nitrite/nitrate system, myeloperoxidase activity and malondialdehyde levels in the rat cerebrum tissue. Material and Methods: Thirty-eight female rats were classified into four groups. Group I (control) (n=11) were fed with standard rat chow; Group IIa, IIb, and IIc with high fat diet for five months. Dehydroepiandrosterone sulphate was administered intraperitoneal as 1 mg/kg for group IIb (n=9) and as 10 mg/kg for group IV (n=9) for seven days. Group I and IIa received the same amount of SF fluid instead. After decapitation, brain tissues were analyzed for malondialdehyde, nitrite/ nitrate, trace element levels and myeloperoxidase activity. Results: Nitrite/nitrate levels were statistically higher in Group IIc than Group I (p&lt;0.001). Malondialdehyde levels were lower in the high fat diet group (p=0.037). Nitrite/nitrate levels were higher and malondialdehyde was significantly lower in group IIc than in group I (p=0.000 and p=0.037, respectively). Conclusions: Dehydroepiandrosterone sulphate in 10 mg/kg dose significantly increases NOx levels and decreases malondialdehyde levels in the cerebral tissues of the rats fed with high fat diet. These findings suggest that the antiatherogenic effect of dehydroepiandrosterone sulphate could be associated with the decrease of the malondialdehyde levels.
Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix ... more Purpose: To examine the effects of an 8-week walking program on serum lipids, circulation matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in post-menopausal women. Method: Body weight, percent body fat, body mass index, estimated maximal oxygen consumption, blood lipids, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 concentrations were assessed and their matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1 molar ratios were calculated in exercise (n=12) and control (n=12) groups. Exercise group completed an eight-week walking program at moderate (~6.29±0.15km/h; ~62% maximum heart rate reserve) intensity. Results: Significant changes in estimated maximal oxygen consumption, systolic and diastolic blood pressures, body weight, and body mass index (p< .05) were determined in exercise group. However, there were no significant changes in the measured blood lipids (triglyceride, cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol), matrix metalloproteinase-9, and tissue inhibitor of metalloproteinase-1 concentrations, and matrix metalloproteinase-9/ tissue inhibitor of metalloproteinase-1 molar ratio of exercise group, except for a nearly significant change in tissue inhibitor of metalloproteinase-1 level (p= .092). We determined no significant changes in any of the measured parameters in control group. Conclusion: Despite its protective effects against coronary heart disease risks, the duration and intensity of this program is not sufficient to cause significant changes in blood lipids, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinase-1 concentrations. A higher-intensity, longer-duration program accompanied with diet is proposed.
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