Delayed hematopoietic reconstitution after cord blood (CB) transplantation (CBT) needs to be over... more Delayed hematopoietic reconstitution after cord blood (CB) transplantation (CBT) needs to be overcome. Bone marrow-derived mesenchymal stem cells (BMMSCs) have been found to enhance engraftment after hematopoietic stem cell transplantation. However, getting BMMSCs involves an invasive procedure. In this study, umbilical cord-derived mesenchymal stem cells (UCMSCs) were isolated from Wharton's jelly and cryopreserved in the UCMSCs bank. Compared with BMMSCs, we found that UCMSCs had superior proliferative potential. We found that NOD/SCID mice cotransplanted with CB and UCMSCs demonstrated significant human CD45 + cell engraftment compared with those transplanted with CB alone. Then, 20 patients with high-risk leukemia were prospectively randomized to either receive cotransplantation of CB and ex vivo expanded banked UCMSCs or to receive CBT alone. No serious adverse events were observed in the patients receiving UCMSC infusion. The time to undergo neutrophil engraftment and platelet engraftment was significantly shorter in the eight patients receiving cotransplantation than that in the 12 patients receiving CBT alone (p = 0.003 and p = 0.004, respectively). Thus, application of ex vivo expanded banked UCMSCs in humans appears to be feasible and safe. UCMSCs can enhance engraftment after CBT, but further studies are warranted.
A series of novel tylophorine-derived dibenzoquinolines has been synthesized and their biological... more A series of novel tylophorine-derived dibenzoquinolines has been synthesized and their biological activity evaluated. Three assays were conducted: inhibition of cancer cell proliferation, inhibition of TGEV replication for anticoronavirus activity, and suppression of nitric oxide production in RAW264.7 cells (a measure of anti-inflammation). The most potent compound from these assays, dibenzoquinoline 33b, showed improved solubility compared to tylophorine 9a, in vivo efficacies in a lung A549 xenografted tumor mouse model and a murine paw edema model, good bioavailability, and no significant neurotoxicity (as tested by a rota-rod test for motor coordination). This is the first study to explore in detail the role of the tylophorine E ring on biological activity and very strongly suggests that tylophorine-derived dibenzoquinolines merit further development into orally active agents.
Background. Delayed hematopoietic reconstitution after cord blood transplantation (CBT) may lead ... more Background. Delayed hematopoietic reconstitution after cord blood transplantation (CBT) may lead to increased risk of complications and longer hospitalization. Bone marrowYderived mesenchymal stem cells (MSCs) have been found to promote engraftment after hematopoietic stem cell transplantation. However, harvesting MSCs from bone marrow involves an invasive procedure. Then again, MSCs can be easily obtained from umbilical cords without harm to the donors. Methods. Umbilical cordYderived MSCs (UCMSCs) were isolated from Wharton's jelly and then ex vivo cultured. After showing normal karyotype and negative for infectious contamination, culture-expanded UCMSCs were intravenously infused into the recipients on the day of CBT. The control patients were those receiving CBT alone. Adverse effects and efficacy of intravenous UCMSCs were evaluated. Results. A total of five patients received cotransplantation of UCMSCs at the time of CBT. No serious adverse events were observed. The time to achieve neutrophil engraftment ranged from 7 to 13 days (median, 11 days) and platelet engraftment ranged from 22 to 41 days (median, 32 days). Compared with the nine patients receiving CBT alone, patients receiving cotransplantation of UCMSCs had significantly faster hematopoietic recovery of neutrophils and platelets (P=0.02 and 0.01, respectively). Conclusions. This pilot study is the first report of cotransplantation of UCMSCs in CBT. Intravenous infusion of UCMSCs appeared to be a feasible and safe modality to enhance hematopoietic engraftment in patients receiving CBT. Further studies were warranted.
Chin J Neurotrauma Surg(Electronic Edition), Jun 15, 2016
Objetive To investigate the effect of bilateral large decompressive craniectomy on late-onset int... more Objetive To investigate the effect of bilateral large decompressive craniectomy on late-onset intracranial hypertension. Methods Seven late-onset cranial pressure patients with cerebral. hernia in Hospital of Jiangxi Provincial Armed Police Corps from January 2010 to January 2015 were selected, they were treated by bilateral large decompressive craniectomy. Results According to GOS classification standards, 5 scores of 3 cases, 4 scores of 4 cases, 3 scores of 1 cases. Conclusion Bilateral large decompressive craniectomy can efficiently reduce the values of ICP, operating in time once discoving conditions changing can imorove the prognosis of those patients. Key words: Bilateral large decompressive craniectomy; Late-onset intracranial hypertension; Severe brain injury
Breast cancer significantly threatens the life of women, while the adverse effects of cancer trea... more Breast cancer significantly threatens the life of women, while the adverse effects of cancer treatment degrade quality of life and psychological well-being. The quality of transitional care following the completion of treatment significantly affects the ability of breast cancer patients to transition successfully into survivorship. This paper introduces multiple theoretical perspectives and provides an overview of the tenets of each in order to identify the positions of breast cancer survivors and to highlight the factors and strategies that influence their transitional care. The theoretical perspectives that are introduced include the social-ecological model, transition theory, and the strengths perspective. In order to improve the holistic care of women with breast cancer, factors relevant to transition are categorized into the individual, interpersonal, organizational, community, and policy levels. Furthermore, empirical interventions, which are based on the respective advantages of the various levels of the social-ecological model, are proposed in order to conform to the sociocultural context and clinical practices. Healthcare providers should leverage the strengths and resources at each level to develop feasible strategies and to provide quality of care in order to assist breast cancer patients to transition successfully from treatment to survivorship and to holistically improve their subsequent quality of life and function.
The Gibbs free energy of a vacuum silicothermic reduction for the production of metallic zinc was... more The Gibbs free energy of a vacuum silicothermic reduction for the production of metallic zinc was calculated and analyzed thermodynamically. The results show that reducing ZnO by silicon is thermodynamically possible at 1100-1500 K. However, about 50% (w) of the ZnO was not reduced because the SiO2 generated by the reduction of ZnO with silicon can react with ZnO and produce 2ZnO• SiO2. Upon the addition of CaO, it can react with SiO2 before ZnO to inhibit the production of 2ZnO•SiO2 and ZnO can be reduced to Zn completely. Slagging reactions and the vacuum technique can be used to lower the Gibbs free energy of the reduction reaction. We carried out experiments to reduce ZnO from hot dip galvanizing ash using silicon. The results showed that the reduction efficiency of ZnO was 92.81% and the metal Zn obtained was well crystallized under the following experimental conditions: a temperature of 1448 K, a vacuum reduction time of 120 min, and a residual gas pressure of 20 Pa. X-ray diffraction (XRD) analysis indicated that the main compound in the slag was 2CaO•SiO2.
The repair of defects of articular cartilage has continued to be a difficult problem. This articl... more The repair of defects of articular cartilage has continued to be a difficult problem. This article provided a collective review from literature pertaining to the advances gained in the repair of cartilaginous defects. In the spontaneous repair, if the defect of the cartilage was less than 3 mm, might result in complete or partial repair, but in those the diameter was more than 3 mm, the defect could not be repaired by normal cartilage. Although the cartilaginous autograft could give good result, but it could not be widely applied because short of supply of the autogenous cartilage. Cartilagious allograft could not be taken to repair cartilaginous defect because of reaction from tissue rejection. The transplantation of periosteal or perichondral graft had been tried but was eventually abandoned because of poor long-term result. The transplantation of free chondrocytes might be a method of hope. In general, transplantation of free chondrocytes into the cartilaginous defect will be lost. The supply of autogenous chondrocytes was very limited, and the heterogenous chondrocytes would inflict immunoreaction after being transplanted. In late of 1980, a new concept of tissue engineering was proposed. The problem that a scaffold of appropriate material which could hold the free chondrocytes in place from being lost might undergo proliferation and differentiation into new cartilage was far from being solved. Although tissue engineering still had various problems needed further investigation, but it will probably be the main direction of development in this field.
Chin J Neurotrauma Surg(Electronic Edition), Jun 15, 2016
Objective To compare the efficacy between lower temporal large decompressive craniectomy and stan... more Objective To compare the efficacy between lower temporal large decompressive craniectomy and standard large trauma craniectomy in the treatment of patients with severe head injury. Methods The data of 173 patients in Armed Police Jiangxi Corps Hospital from January 2008 to March 2014 with severe head injury, who received lower temporal large decompressive craniectomy(the research group)or standard large trauma craniectomy(the control group), were retrospectively analyzed. Lower temporal large decompressive craniectomy was conducted in the research group(92 cases), while standard large trauma craniectomy was conducted in the control group(81 cases). GCS rating, incidence rate of complications, prognosis and other indicators were observed and compared between two groups. Results At 6 months after surgery, no statistically significant differences were reported between these two groups in terms of good recovery, moderate disability, severe disability, vegetative state and dead as defined by GOS rating (27.2%, 15.2%, 29.3%, 8.7% and 19.6% in the research group vs. 22.2%, 21.0%, 23.5%, 7.4% and 25.9% in the control group, respectively) (P>0.05). The average incidence rate of postoperative complications (8.1%) in the research group was lower than that in the control group (11.8%), suggesting a statistically significant difference (P<0.05). Conclusion In the treatment of severe head injury, lower temporal large decompressive craniectomy can achieve a comparable prognosis to standard large trauma craniectomy while effectively reduce the complications, demonstrating it is worth clinical promotion. Key words: Severe head injury; Lower temporal large decompressive craniectomy; Standard large trauma craniectomy
Academic Journal of Second Military Medical University, 2006
Objective:To investigate the influence of high expression of Retn gene on glucose uptake by 3T3-L... more Objective:To investigate the influence of high expression of Retn gene on glucose uptake by 3T3-L1 cells and to study its mechanism in inducing insulin resistance.Methods:(1)Radioimmunoassay was used to determine the glucose uptake by 3T3-L1 cells with low-,normal-and high-level Retn expression under basal and insulin-stimulated states.(2)RT-PCR and real-time RT-PCR were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different expression of Retn,including insulin receptor substrate-1(IRS-1),phosphatidylinositol 3-kinase(PI-3K),AKT-2,glucose transporter-4(GLUT-4),p38 mitogen-activated protein kinase(p38MAPK),and glycogen synthase kinase-3β(GSK-3β).(Results:) The uptake of glucose decreased with the increase of Retn expression under basal and insulin-stimulated conditions.The mRNA expression of 2 signal protein PI-3K and AKT-2 decreased with the increase of Retn expression;and the expression of GSK-3β and p38MAPK increased with the increase of Retn expression.Conclusion: Resistin protein can induce insulin resistance in adipocytes,which might be related to the expression changes of some proteins in PI-3K and Ras pathways.
This paper reviewed the applications of germplasm improvement in several medical plants via moder... more This paper reviewed the applications of germplasm improvement in several medical plants via modern breeding techniques,such as transgenic techniques,molecular marker selection,induced mutation and tissue and cell engineering,etc.Meanwhile,the future trend of breeding techniques and germplasm improvement in medical plants were also briefly introduced and discussed,which will provide the reference for other similar researches.
Cytomegalovirus (CMV) retinitis comorbid with diabetic retinopathy is uncommon. We report a case ... more Cytomegalovirus (CMV) retinitis comorbid with diabetic retinopathy is uncommon. We report a case of bilateral CMV retinitis and diabetic retinopathy in a patient who underwent pancreas transplantation and share the experience of the treatment outcome. An 18-year-old male diagnosed with type-1 diabetes mellitus received pancreas transplantation and immunosuppressive therapy suffered from progressively blurred vision in both eyes for several days. His visual acuity was 20/100 in the right eye and 20/50 in the left eye. Ophthalmic examination revealed bilateral diabetic macular edema (DME) without intraocular inflammatory signs in either eye. The DME subsided after 2 monthly intravitreal injections of aflibercept. However, bilateral panuveitis with CMV retinitis was observed after antivascular endothelial growth factor therapy. The retinitis subsided gradually but completely after systemic and intravitreal antiviral therapy. However, bilateral DME recurred and persisted despite repeated injections of aflibercept during the resting follow-up period. Our case suggests that CMV retinitis can coexist with other retinal diseases, including diabetic retinopathy. Treatment is difficult in such cases.
A hydrogel sensing film for a real-time and indicator-free detection of Zn 2+ is developed by emb... more A hydrogel sensing film for a real-time and indicator-free detection of Zn 2+ is developed by embedding a fluorescent indicator 11,16-bis(phenyl)-6,6,21,21-tetramethyl-m-benzi-6,21porphodimethene in a hydrogel host poly(2-hydroxyethyl methacrylate). The sensing film shows high stability and selectivity to Zn 2+. The sensitivity of the sensing film is increased by fabricating a micron-sized pillar array on the surface of the sensing film to increase the surface area. For Zn 2+ concentrations of 10 À4 and 10 À3 M, the response time is 30 and 3 s, respectively.
Semiconductor Science and Technology, Apr 27, 2015
In this paper, we investigate the key issues in raising the on/off current ratio and increasing t... more In this paper, we investigate the key issues in raising the on/off current ratio and increasing the output current. A 1 V operated inverter composed of an enhancement-mode space-chargelimited transistor (SCLT) and a depletion-mode SCLT is demonstrated using the self-assembled monolayer modulation process. With a bulk-conduction mechanism, good bias-stress reliability, and good bending durability are obtained. Finally, key scaling-up processes, including nanoimprinting and blade-coated nanospheres, are demonstrated.
A pulse-modulated direct-current methane plasma is used to deposit amorphous hydrogenated carbon ... more A pulse-modulated direct-current methane plasma is used to deposit amorphous hydrogenated carbon (a-C:H) films on Si and polymethyl methacrylate (PMMA) substrates. The structure and mechanical properties of the films are examined by applying a negative pulse bias voltage of 0.5 to 3 kV to the substrate at a pulse bias period of 100 to 200 μs. The deposition rate on both Si and PMMA increases with increasing the net input power, independent of the pulse period. The Raman spectra demonstrate that the films on Si are diamond-like carbon (DLC), while those on PMMA are polymer-like or soft amorphous carbon because of higher crystallinity of the sp 2 phase and lower nanoscale hardness. The residual compressive stress of the films on PMMA is constantly low ranging from 0 to 2 GPa due exclusively to high flexibility of PMMA, which causes the easy relief of the stress and thus the density decrease in the films.
μ-Opioid receptor (MOR) agonists are analgesics used clinically for the treatment of moderate to ... more μ-Opioid receptor (MOR) agonists are analgesics used clinically for the treatment of moderate to severe pain, but their use is associated with severe adverse effects such as respiratory depression, constipation, tolerance, dependence, and rewarding effects. In this study, we identified N-({2-[(4-bromo-2-trifluoromethoxyphenyl)sulfonyl]-1,2,3,4-tetrahydro-1-isoquinolinyl}methyl)cyclohexan ecarboxamide (1) as a novel opioid receptor agonist by high-throughput screening. Structural modifications made to 1 to improve potency and blood-brain-barrier (BBB) penetration resulted in compounds 45 and 46. Compound 45 was a potent MOR/KOR (κ-opioid receptor) agonist, and compound 46 was a potent MOR and medium KOR agonist. Both 45 and 46 demonstrated a significant anti-nociceptive effect in a tail-flick test performed in wild type (WT) B6 mice. The ED 50 value of 46 was 1.059 mg/kg, and the brain concentrations of 45 and 46 were 7424 and 11696 ng/g, respectively. Accordingly, compounds 45 and 46 are proposed for lead optimization and in vivo disease-related pain studies. Keywords µ-opioid receptor agonist κ-opioid receptor agonist structure-activity relationship blood-brain barrier penetration anti-nociceptive effects tail-flick test However, the use of morphine comes with severe adverse effects, such as respiratory depression, constipation, tolerance, dependence, and rewarding effects. Furthermore, the misuse and abuse of opioids causes public health problems worldwide. Recently, the U.S. Food and Drug Administration announced the "Abuse-Deterrent Opioids-Evaluation and Labeling Guidance for Industry" to encourage the development of abuse-deterrent formulations of opioids and novel approaches with fewer adverse effects for pain treatment [6].The analgesic effect of most opioids in clinical use has been identified as the activation of MOR principally exerts analgesic effect, and they are either non-selective or selective MOR agonists with similar drawbacks of adverse effects to morphine [7]. Recently, several selective opioid receptor ligands have been discovered, such as a selective KOR agonist [8-10], a mixed MOR agonist/DOR (δ-opioid receptor) agonist [11-13], a mixed MOR agonist/DOR antagonist [14], and a mixed MOR agonist/KOR agonist [15-18], which may hold the key to novel opioid based drugs that can impart the analgesic properties of morphine, without the adverse effects. For example, a KOR selective agonist, TRK820, can suppress the morphine-induced rewarding and locomotor-enhancing effects [19]. Another KOR selective agonist, U69593, prevents the sensitization of cocaine-induced rewarding effects [20]. In addition, several neurobiology studies have illuminated the 20 R = piperidinyl 21 R = morpholinyl 22 R = pyrrolidinyl Scheme 2. Reagents and conditions: (a) Boc 2 O, NaHCO 3 , CH 2 Cl 2 , rt, 1 h (100 %). (b) N 2 H 4 (99 %), CH 3 CN, reflux, 32 h (100 %). (c) acyl carbamate, Et 3 N, CH 2 Cl 2 , rt, 8 h (91-95 %). (d) TFA, CH 2 Cl 2 , rt, 3 h (41-99 %). 31 0.37 39 Cl Cl 0.25 a EC 50 values are the means of at least three independent experiments. 42 CH 2 CH 2 Cl 0.03 43 CH 3 0.04
Vocal psychology belongs to the branch of music psychology, which is the cross-study of vocal art... more Vocal psychology belongs to the branch of music psychology, which is the cross-study of vocal art and psychology, and is also a new discipline with both theory and application. Vocal singing uses a thinking, conscious person as an instrument that is necessarily governed by the psyche over the physiology, relying on the brain to direct the movement of the singing muscles and the coordination of the vocal organs. The purpose of this thesis is to explore the application of vocal psychology in vocal singing and teaching, to explain the generation and development of various psychological phenomena in singing activities, to reveal the role and significance of various psychological factors, to provide singers with a theoretical basis for psychological aspects, and to correctly understand the scientific laws of the inner psychology of vocal singing. The effectiveness of classroom teaching is reflected in effective and efficient aspects. The effectiveness of a vocal lesson can be measured by the criteria of whether the teaching is oriented, scientific, artistic, and efficient. Effective teaching design is the basis of teaching effectiveness, elaborate teaching organization is the guarantee of teaching effectiveness, and flexible teaching methods are the root of teaching effectiveness; all three need to be closely combined and organically unified. Effective teaching design is a holistic thinking before the implementation of teaching; all factors related to teaching, practice, and evaluation should be fully considered in the teaching design; teachers should take the learning effect of students and the cultivation of employability as the starting point for effective teaching design; and the classroom teaching of "vocal music" is a "process" and teachers should teach in accordance with the teaching design. They should focus on guiding students to experience and cultivate their abilities in a series of "processes" such as the emotion of vocal music, the teaching situation, the effect of listening, the creation of expression, and the aesthetic value. In addition, teachers should combine the teaching methods of transmission and inspiration, classroom teaching, and after-school training and combine relatively fixed teaching methods with flexible teaching methods to maximize the effectiveness of teaching.
Delayed hematopoietic reconstitution after cord blood (CB) transplantation (CBT) needs to be over... more Delayed hematopoietic reconstitution after cord blood (CB) transplantation (CBT) needs to be overcome. Bone marrow-derived mesenchymal stem cells (BMMSCs) have been found to enhance engraftment after hematopoietic stem cell transplantation. However, getting BMMSCs involves an invasive procedure. In this study, umbilical cord-derived mesenchymal stem cells (UCMSCs) were isolated from Wharton's jelly and cryopreserved in the UCMSCs bank. Compared with BMMSCs, we found that UCMSCs had superior proliferative potential. We found that NOD/SCID mice cotransplanted with CB and UCMSCs demonstrated significant human CD45 + cell engraftment compared with those transplanted with CB alone. Then, 20 patients with high-risk leukemia were prospectively randomized to either receive cotransplantation of CB and ex vivo expanded banked UCMSCs or to receive CBT alone. No serious adverse events were observed in the patients receiving UCMSC infusion. The time to undergo neutrophil engraftment and platelet engraftment was significantly shorter in the eight patients receiving cotransplantation than that in the 12 patients receiving CBT alone (p = 0.003 and p = 0.004, respectively). Thus, application of ex vivo expanded banked UCMSCs in humans appears to be feasible and safe. UCMSCs can enhance engraftment after CBT, but further studies are warranted.
A series of novel tylophorine-derived dibenzoquinolines has been synthesized and their biological... more A series of novel tylophorine-derived dibenzoquinolines has been synthesized and their biological activity evaluated. Three assays were conducted: inhibition of cancer cell proliferation, inhibition of TGEV replication for anticoronavirus activity, and suppression of nitric oxide production in RAW264.7 cells (a measure of anti-inflammation). The most potent compound from these assays, dibenzoquinoline 33b, showed improved solubility compared to tylophorine 9a, in vivo efficacies in a lung A549 xenografted tumor mouse model and a murine paw edema model, good bioavailability, and no significant neurotoxicity (as tested by a rota-rod test for motor coordination). This is the first study to explore in detail the role of the tylophorine E ring on biological activity and very strongly suggests that tylophorine-derived dibenzoquinolines merit further development into orally active agents.
Background. Delayed hematopoietic reconstitution after cord blood transplantation (CBT) may lead ... more Background. Delayed hematopoietic reconstitution after cord blood transplantation (CBT) may lead to increased risk of complications and longer hospitalization. Bone marrowYderived mesenchymal stem cells (MSCs) have been found to promote engraftment after hematopoietic stem cell transplantation. However, harvesting MSCs from bone marrow involves an invasive procedure. Then again, MSCs can be easily obtained from umbilical cords without harm to the donors. Methods. Umbilical cordYderived MSCs (UCMSCs) were isolated from Wharton's jelly and then ex vivo cultured. After showing normal karyotype and negative for infectious contamination, culture-expanded UCMSCs were intravenously infused into the recipients on the day of CBT. The control patients were those receiving CBT alone. Adverse effects and efficacy of intravenous UCMSCs were evaluated. Results. A total of five patients received cotransplantation of UCMSCs at the time of CBT. No serious adverse events were observed. The time to achieve neutrophil engraftment ranged from 7 to 13 days (median, 11 days) and platelet engraftment ranged from 22 to 41 days (median, 32 days). Compared with the nine patients receiving CBT alone, patients receiving cotransplantation of UCMSCs had significantly faster hematopoietic recovery of neutrophils and platelets (P=0.02 and 0.01, respectively). Conclusions. This pilot study is the first report of cotransplantation of UCMSCs in CBT. Intravenous infusion of UCMSCs appeared to be a feasible and safe modality to enhance hematopoietic engraftment in patients receiving CBT. Further studies were warranted.
Chin J Neurotrauma Surg(Electronic Edition), Jun 15, 2016
Objetive To investigate the effect of bilateral large decompressive craniectomy on late-onset int... more Objetive To investigate the effect of bilateral large decompressive craniectomy on late-onset intracranial hypertension. Methods Seven late-onset cranial pressure patients with cerebral. hernia in Hospital of Jiangxi Provincial Armed Police Corps from January 2010 to January 2015 were selected, they were treated by bilateral large decompressive craniectomy. Results According to GOS classification standards, 5 scores of 3 cases, 4 scores of 4 cases, 3 scores of 1 cases. Conclusion Bilateral large decompressive craniectomy can efficiently reduce the values of ICP, operating in time once discoving conditions changing can imorove the prognosis of those patients. Key words: Bilateral large decompressive craniectomy; Late-onset intracranial hypertension; Severe brain injury
Breast cancer significantly threatens the life of women, while the adverse effects of cancer trea... more Breast cancer significantly threatens the life of women, while the adverse effects of cancer treatment degrade quality of life and psychological well-being. The quality of transitional care following the completion of treatment significantly affects the ability of breast cancer patients to transition successfully into survivorship. This paper introduces multiple theoretical perspectives and provides an overview of the tenets of each in order to identify the positions of breast cancer survivors and to highlight the factors and strategies that influence their transitional care. The theoretical perspectives that are introduced include the social-ecological model, transition theory, and the strengths perspective. In order to improve the holistic care of women with breast cancer, factors relevant to transition are categorized into the individual, interpersonal, organizational, community, and policy levels. Furthermore, empirical interventions, which are based on the respective advantages of the various levels of the social-ecological model, are proposed in order to conform to the sociocultural context and clinical practices. Healthcare providers should leverage the strengths and resources at each level to develop feasible strategies and to provide quality of care in order to assist breast cancer patients to transition successfully from treatment to survivorship and to holistically improve their subsequent quality of life and function.
The Gibbs free energy of a vacuum silicothermic reduction for the production of metallic zinc was... more The Gibbs free energy of a vacuum silicothermic reduction for the production of metallic zinc was calculated and analyzed thermodynamically. The results show that reducing ZnO by silicon is thermodynamically possible at 1100-1500 K. However, about 50% (w) of the ZnO was not reduced because the SiO2 generated by the reduction of ZnO with silicon can react with ZnO and produce 2ZnO• SiO2. Upon the addition of CaO, it can react with SiO2 before ZnO to inhibit the production of 2ZnO•SiO2 and ZnO can be reduced to Zn completely. Slagging reactions and the vacuum technique can be used to lower the Gibbs free energy of the reduction reaction. We carried out experiments to reduce ZnO from hot dip galvanizing ash using silicon. The results showed that the reduction efficiency of ZnO was 92.81% and the metal Zn obtained was well crystallized under the following experimental conditions: a temperature of 1448 K, a vacuum reduction time of 120 min, and a residual gas pressure of 20 Pa. X-ray diffraction (XRD) analysis indicated that the main compound in the slag was 2CaO•SiO2.
The repair of defects of articular cartilage has continued to be a difficult problem. This articl... more The repair of defects of articular cartilage has continued to be a difficult problem. This article provided a collective review from literature pertaining to the advances gained in the repair of cartilaginous defects. In the spontaneous repair, if the defect of the cartilage was less than 3 mm, might result in complete or partial repair, but in those the diameter was more than 3 mm, the defect could not be repaired by normal cartilage. Although the cartilaginous autograft could give good result, but it could not be widely applied because short of supply of the autogenous cartilage. Cartilagious allograft could not be taken to repair cartilaginous defect because of reaction from tissue rejection. The transplantation of periosteal or perichondral graft had been tried but was eventually abandoned because of poor long-term result. The transplantation of free chondrocytes might be a method of hope. In general, transplantation of free chondrocytes into the cartilaginous defect will be lost. The supply of autogenous chondrocytes was very limited, and the heterogenous chondrocytes would inflict immunoreaction after being transplanted. In late of 1980, a new concept of tissue engineering was proposed. The problem that a scaffold of appropriate material which could hold the free chondrocytes in place from being lost might undergo proliferation and differentiation into new cartilage was far from being solved. Although tissue engineering still had various problems needed further investigation, but it will probably be the main direction of development in this field.
Chin J Neurotrauma Surg(Electronic Edition), Jun 15, 2016
Objective To compare the efficacy between lower temporal large decompressive craniectomy and stan... more Objective To compare the efficacy between lower temporal large decompressive craniectomy and standard large trauma craniectomy in the treatment of patients with severe head injury. Methods The data of 173 patients in Armed Police Jiangxi Corps Hospital from January 2008 to March 2014 with severe head injury, who received lower temporal large decompressive craniectomy(the research group)or standard large trauma craniectomy(the control group), were retrospectively analyzed. Lower temporal large decompressive craniectomy was conducted in the research group(92 cases), while standard large trauma craniectomy was conducted in the control group(81 cases). GCS rating, incidence rate of complications, prognosis and other indicators were observed and compared between two groups. Results At 6 months after surgery, no statistically significant differences were reported between these two groups in terms of good recovery, moderate disability, severe disability, vegetative state and dead as defined by GOS rating (27.2%, 15.2%, 29.3%, 8.7% and 19.6% in the research group vs. 22.2%, 21.0%, 23.5%, 7.4% and 25.9% in the control group, respectively) (P>0.05). The average incidence rate of postoperative complications (8.1%) in the research group was lower than that in the control group (11.8%), suggesting a statistically significant difference (P<0.05). Conclusion In the treatment of severe head injury, lower temporal large decompressive craniectomy can achieve a comparable prognosis to standard large trauma craniectomy while effectively reduce the complications, demonstrating it is worth clinical promotion. Key words: Severe head injury; Lower temporal large decompressive craniectomy; Standard large trauma craniectomy
Academic Journal of Second Military Medical University, 2006
Objective:To investigate the influence of high expression of Retn gene on glucose uptake by 3T3-L... more Objective:To investigate the influence of high expression of Retn gene on glucose uptake by 3T3-L1 cells and to study its mechanism in inducing insulin resistance.Methods:(1)Radioimmunoassay was used to determine the glucose uptake by 3T3-L1 cells with low-,normal-and high-level Retn expression under basal and insulin-stimulated states.(2)RT-PCR and real-time RT-PCR were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different expression of Retn,including insulin receptor substrate-1(IRS-1),phosphatidylinositol 3-kinase(PI-3K),AKT-2,glucose transporter-4(GLUT-4),p38 mitogen-activated protein kinase(p38MAPK),and glycogen synthase kinase-3β(GSK-3β).(Results:) The uptake of glucose decreased with the increase of Retn expression under basal and insulin-stimulated conditions.The mRNA expression of 2 signal protein PI-3K and AKT-2 decreased with the increase of Retn expression;and the expression of GSK-3β and p38MAPK increased with the increase of Retn expression.Conclusion: Resistin protein can induce insulin resistance in adipocytes,which might be related to the expression changes of some proteins in PI-3K and Ras pathways.
This paper reviewed the applications of germplasm improvement in several medical plants via moder... more This paper reviewed the applications of germplasm improvement in several medical plants via modern breeding techniques,such as transgenic techniques,molecular marker selection,induced mutation and tissue and cell engineering,etc.Meanwhile,the future trend of breeding techniques and germplasm improvement in medical plants were also briefly introduced and discussed,which will provide the reference for other similar researches.
Cytomegalovirus (CMV) retinitis comorbid with diabetic retinopathy is uncommon. We report a case ... more Cytomegalovirus (CMV) retinitis comorbid with diabetic retinopathy is uncommon. We report a case of bilateral CMV retinitis and diabetic retinopathy in a patient who underwent pancreas transplantation and share the experience of the treatment outcome. An 18-year-old male diagnosed with type-1 diabetes mellitus received pancreas transplantation and immunosuppressive therapy suffered from progressively blurred vision in both eyes for several days. His visual acuity was 20/100 in the right eye and 20/50 in the left eye. Ophthalmic examination revealed bilateral diabetic macular edema (DME) without intraocular inflammatory signs in either eye. The DME subsided after 2 monthly intravitreal injections of aflibercept. However, bilateral panuveitis with CMV retinitis was observed after antivascular endothelial growth factor therapy. The retinitis subsided gradually but completely after systemic and intravitreal antiviral therapy. However, bilateral DME recurred and persisted despite repeated injections of aflibercept during the resting follow-up period. Our case suggests that CMV retinitis can coexist with other retinal diseases, including diabetic retinopathy. Treatment is difficult in such cases.
A hydrogel sensing film for a real-time and indicator-free detection of Zn 2+ is developed by emb... more A hydrogel sensing film for a real-time and indicator-free detection of Zn 2+ is developed by embedding a fluorescent indicator 11,16-bis(phenyl)-6,6,21,21-tetramethyl-m-benzi-6,21porphodimethene in a hydrogel host poly(2-hydroxyethyl methacrylate). The sensing film shows high stability and selectivity to Zn 2+. The sensitivity of the sensing film is increased by fabricating a micron-sized pillar array on the surface of the sensing film to increase the surface area. For Zn 2+ concentrations of 10 À4 and 10 À3 M, the response time is 30 and 3 s, respectively.
Semiconductor Science and Technology, Apr 27, 2015
In this paper, we investigate the key issues in raising the on/off current ratio and increasing t... more In this paper, we investigate the key issues in raising the on/off current ratio and increasing the output current. A 1 V operated inverter composed of an enhancement-mode space-chargelimited transistor (SCLT) and a depletion-mode SCLT is demonstrated using the self-assembled monolayer modulation process. With a bulk-conduction mechanism, good bias-stress reliability, and good bending durability are obtained. Finally, key scaling-up processes, including nanoimprinting and blade-coated nanospheres, are demonstrated.
A pulse-modulated direct-current methane plasma is used to deposit amorphous hydrogenated carbon ... more A pulse-modulated direct-current methane plasma is used to deposit amorphous hydrogenated carbon (a-C:H) films on Si and polymethyl methacrylate (PMMA) substrates. The structure and mechanical properties of the films are examined by applying a negative pulse bias voltage of 0.5 to 3 kV to the substrate at a pulse bias period of 100 to 200 μs. The deposition rate on both Si and PMMA increases with increasing the net input power, independent of the pulse period. The Raman spectra demonstrate that the films on Si are diamond-like carbon (DLC), while those on PMMA are polymer-like or soft amorphous carbon because of higher crystallinity of the sp 2 phase and lower nanoscale hardness. The residual compressive stress of the films on PMMA is constantly low ranging from 0 to 2 GPa due exclusively to high flexibility of PMMA, which causes the easy relief of the stress and thus the density decrease in the films.
μ-Opioid receptor (MOR) agonists are analgesics used clinically for the treatment of moderate to ... more μ-Opioid receptor (MOR) agonists are analgesics used clinically for the treatment of moderate to severe pain, but their use is associated with severe adverse effects such as respiratory depression, constipation, tolerance, dependence, and rewarding effects. In this study, we identified N-({2-[(4-bromo-2-trifluoromethoxyphenyl)sulfonyl]-1,2,3,4-tetrahydro-1-isoquinolinyl}methyl)cyclohexan ecarboxamide (1) as a novel opioid receptor agonist by high-throughput screening. Structural modifications made to 1 to improve potency and blood-brain-barrier (BBB) penetration resulted in compounds 45 and 46. Compound 45 was a potent MOR/KOR (κ-opioid receptor) agonist, and compound 46 was a potent MOR and medium KOR agonist. Both 45 and 46 demonstrated a significant anti-nociceptive effect in a tail-flick test performed in wild type (WT) B6 mice. The ED 50 value of 46 was 1.059 mg/kg, and the brain concentrations of 45 and 46 were 7424 and 11696 ng/g, respectively. Accordingly, compounds 45 and 46 are proposed for lead optimization and in vivo disease-related pain studies. Keywords µ-opioid receptor agonist κ-opioid receptor agonist structure-activity relationship blood-brain barrier penetration anti-nociceptive effects tail-flick test However, the use of morphine comes with severe adverse effects, such as respiratory depression, constipation, tolerance, dependence, and rewarding effects. Furthermore, the misuse and abuse of opioids causes public health problems worldwide. Recently, the U.S. Food and Drug Administration announced the "Abuse-Deterrent Opioids-Evaluation and Labeling Guidance for Industry" to encourage the development of abuse-deterrent formulations of opioids and novel approaches with fewer adverse effects for pain treatment [6].The analgesic effect of most opioids in clinical use has been identified as the activation of MOR principally exerts analgesic effect, and they are either non-selective or selective MOR agonists with similar drawbacks of adverse effects to morphine [7]. Recently, several selective opioid receptor ligands have been discovered, such as a selective KOR agonist [8-10], a mixed MOR agonist/DOR (δ-opioid receptor) agonist [11-13], a mixed MOR agonist/DOR antagonist [14], and a mixed MOR agonist/KOR agonist [15-18], which may hold the key to novel opioid based drugs that can impart the analgesic properties of morphine, without the adverse effects. For example, a KOR selective agonist, TRK820, can suppress the morphine-induced rewarding and locomotor-enhancing effects [19]. Another KOR selective agonist, U69593, prevents the sensitization of cocaine-induced rewarding effects [20]. In addition, several neurobiology studies have illuminated the 20 R = piperidinyl 21 R = morpholinyl 22 R = pyrrolidinyl Scheme 2. Reagents and conditions: (a) Boc 2 O, NaHCO 3 , CH 2 Cl 2 , rt, 1 h (100 %). (b) N 2 H 4 (99 %), CH 3 CN, reflux, 32 h (100 %). (c) acyl carbamate, Et 3 N, CH 2 Cl 2 , rt, 8 h (91-95 %). (d) TFA, CH 2 Cl 2 , rt, 3 h (41-99 %). 31 0.37 39 Cl Cl 0.25 a EC 50 values are the means of at least three independent experiments. 42 CH 2 CH 2 Cl 0.03 43 CH 3 0.04
Vocal psychology belongs to the branch of music psychology, which is the cross-study of vocal art... more Vocal psychology belongs to the branch of music psychology, which is the cross-study of vocal art and psychology, and is also a new discipline with both theory and application. Vocal singing uses a thinking, conscious person as an instrument that is necessarily governed by the psyche over the physiology, relying on the brain to direct the movement of the singing muscles and the coordination of the vocal organs. The purpose of this thesis is to explore the application of vocal psychology in vocal singing and teaching, to explain the generation and development of various psychological phenomena in singing activities, to reveal the role and significance of various psychological factors, to provide singers with a theoretical basis for psychological aspects, and to correctly understand the scientific laws of the inner psychology of vocal singing. The effectiveness of classroom teaching is reflected in effective and efficient aspects. The effectiveness of a vocal lesson can be measured by the criteria of whether the teaching is oriented, scientific, artistic, and efficient. Effective teaching design is the basis of teaching effectiveness, elaborate teaching organization is the guarantee of teaching effectiveness, and flexible teaching methods are the root of teaching effectiveness; all three need to be closely combined and organically unified. Effective teaching design is a holistic thinking before the implementation of teaching; all factors related to teaching, practice, and evaluation should be fully considered in the teaching design; teachers should take the learning effect of students and the cultivation of employability as the starting point for effective teaching design; and the classroom teaching of "vocal music" is a "process" and teachers should teach in accordance with the teaching design. They should focus on guiding students to experience and cultivate their abilities in a series of "processes" such as the emotion of vocal music, the teaching situation, the effect of listening, the creation of expression, and the aesthetic value. In addition, teachers should combine the teaching methods of transmission and inspiration, classroom teaching, and after-school training and combine relatively fixed teaching methods with flexible teaching methods to maximize the effectiveness of teaching.
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