Papers by Yoshiki Sekijima
Scientific Reports, Apr 26, 2022
Rinshō shinkeigaku, 2014
Rheumatology International, Jul 24, 2020
European Journal of Neurology, Oct 15, 2019
European Journal of Echocardiography, Feb 11, 2016
Journal of the Neurological Sciences, Feb 1, 2012
Neuroradiology, Jun 16, 2022
Internal Medicine, Dec 1, 2019
Amyotrophic Lateral Sclerosis, Sep 1, 2012
Clinical and Experimental Immunology, Aug 12, 2021
We investigated the characteristics of regulatory T cells in adult-onset Still's disease (AOS... more We investigated the characteristics of regulatory T cells in adult-onset Still's disease (AOSD) with a focus on their plasticity, stability and relationship to disease severity. The proportion of circulating CD4+CD25+forkhead box protein 3 (FoxP3+) cells (Tregs) and intracellular expression of effector cytokines, including interferon (IFN)-γ, interleukin (IL)-17 and IL-4, was analysed in 27 untreated patients with AOSD (acute AOSD), 11 of the 27 patients after remission and 16 healthy controls (HC) using flow cytometry. The suppressive ability of Tregs was also evaluated. Regression analyses of the results were performed. The proportion of Tregs was significantly lower in patients with acute AOSD than in the HC. The expression levels of IFN-γ, IL-17 and IL-4 in Tregs were significantly increased in patients with acute AOSD. IFN-γ and IL-4 expression levels were inversely correlated with the proportion of Tregs and positively correlated with serum ferritin levels. Decreased expression of FoxP3 in CD4+CD25+ cells, which was correlated with increased expression of IL-17, and impaired suppressive function were observed in Tregs in acute AOSD. However, these aberrant findings in Tregs, including the reduced circulating proportion and functional ability and altered intracellular expression levels of cytokines and FoxP3, were significantly improved after remission. In acute AOSD, Tregs show plastic changes, including effector cytokine production and reductions in their proportion and functional activity. IFN-γ and IL-4 expression levels in Tregs may be associated with disease severity. Also, down-regulation of FoxP3 may be related to IL-17 expression in Tregs. Importantly, the stability of Tregs can be restored in remission.
Amyloid, May 15, 2012
We describe a rare complication, systemic arterial thromboembolism, seen in two patients with sen... more We describe a rare complication, systemic arterial thromboembolism, seen in two patients with senile systemic amyloidosis (SSA). Case 1 was a 73-year-old man who was tentatively diagnosed as having cardiac amyloidosis. Five months later, he was afflicted by severe left flank pain. CT disclosed renal infarction and then he received endomyocardial biopsy and the transthyretin (TTR) gene analysis, leading to the final diagnosis of SSA. Case 2 was an 88-year-old woman who had been definitively diagnosed as having SSA-related heart failure with atrial fibrillation two years before. She was transferred to the emergency room in our hospital and enhanced CT revealed complete occlusions of the left internal carotid and left vertebral arteries, both subclavian arteries, and the left renal and left internal iliac arteries. Paying much attention to intracardiac thrombosis might be necessary in taking care of SSA patients.
International Journal of Molecular Sciences, Jan 22, 2018
European Journal of Nuclear Medicine and Molecular Imaging, Sep 10, 2017
Annals of Neurology, Feb 1, 1997
To investigate the effect of the overexpression of β‐amyloid precursor protein (APP) on the produ... more To investigate the effect of the overexpression of β‐amyloid precursor protein (APP) on the production of two major amyloid β protein (Aβ) species, Aβ40 and Aβ42(43), we measured amounts of Aβ1–40 and Aβ1–42(43) in the plasma from 44 patients with Down's syndrome (DS) (age, 19–61 years) and 66 age‐matched normal controls using enzyme‐linked immunosorbent assays. Plasma concentrations of both Aβ1–40 and Aβ1–42(43) were increased about 3‐fold and 2‐fold, respectively, in DS patients compared with normal controls. Especially, the increases in plasma Aβ1–40 in DS Patients were statistically higher than the 1.5‐fold increase one might predict based on the gene dose of APP in DS. These findings showed that both Aβ1–40 and Aβ1–42(43) are increased in plasma in DS patients, the former more than the latter, suggesting that overexpression of APP and/or other genes may have different effects on the production of these two Aβ species in DS.
Research Square (Research Square), Jan 16, 2023
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Papers by Yoshiki Sekijima