HAL (Le Centre pour la Communication Scientifique Directe), Jun 14, 2023
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Ten novel polymorphic microsatellites (seven with perfect motifs) were isolated from vairone spec... more Ten novel polymorphic microsatellites (seven with perfect motifs) were isolated from vairone species (Telestes souffia and Telestes muticellus), which are endangered European cyprinid species. Together with 11 previously published cyprinid-specific loci, five multiplex sets were optimized, allowing the genotyping of 21 polymorphic loci. The level of genetic diversity was assessed in 97 individuals from the two species T. souffia and T. muticellus. We also successfully applied the 21 microsatellites to nine related species. These primers will thus be useful in assessing population structure of the vairone and other cyprinid species, with application for conservation issues and phylogeographical approaches.
Ten novel polymorphic microsatellites were isolated from the dace complex (Leuciscus leuciscus), ... more Ten novel polymorphic microsatellites were isolated from the dace complex (Leuciscus leuciscus), which is a European cyprinid species. Four multiplex polymerase chain reaction sets were optimized in order to genotype 26 polymorphic loci in all, including 16 previously published cyprinid-specific loci. The level of genetic diversity was assessed in 142 dace individuals. We also successfully applied 26 of the microsatellites to 10 related species. These primers thus will be useful to assess population structure of the dace and other cyprinid species, with application for conservation issues and phylogeographical approaches.
Aquatic Conservation: Marine and Freshwater Ecosystems
Intraspecific variation in life histories and its environmental correlates can indicate the degre... more Intraspecific variation in life histories and its environmental correlates can indicate the degree of vulnerability to extinction of endangered taxa and guide conservation actions. Zingel asper (Percidae) is an endangered fish endemic to the Rhône catchment (France and Switzerland), where five populations subsist in separate river systems (Loue, Beaume, Durance, Verdon, and Doubs). Two populations of Z. asper differ in growth and longevity, but the existence of broader intraspecific differentiation in life histories and the environmental origins of this variation (if any) remain unknown. The age structure and growth profile of four populations of Z. asper (Loue, Beaume, Durance, and Verdon) and nine additional sub‐populations within the Durance system were determined by scale‐reading analysis, before evaluating the contribution of measured variation in substrate quality, hydraulics, prey availability, and water temperature to growth differentiation among and within populations. A tr...
Le peuplement et la mise en place de la population de l'ile de La Reunion, a l'instar des... more Le peuplement et la mise en place de la population de l'ile de La Reunion, a l'instar des Antilles ou de la Guyane francaises, sont intimement lies aux apports issus de la Traite des esclaves. Alors que les populations, concernees par la Traite atlantique furent en grande majorite originaires de l'Afrique de l'Ouest, les donnees historiques concernant La Reunion montrent clairement que ce fut la cote Est Africaine et Madagascar qui furent les regions les plus sollicitees. Cependant, certaines zones d'ombre persistent concernant l'origine geographique des esclaves africains importes a La Reunion, mais aussi les contributions respectives de Madagascar et de l'Afrique subsaharienne au pool afro-malgache reunionnais. A travers l'analyse des polymorphismes de l'ADN mitochondrial d'un echantillon de la Population reunionnaise, nous apportons ici des elements de reponse a ces deux questions. Nos analyses ont aussi permis d'explorer la dynamique t...
Dietary studies are critical for understanding foraging strategies and have important application... more Dietary studies are critical for understanding foraging strategies and have important applications in conservation and habitat management. We applied a robust metabarcoding protocol to characterize the diet of the critically endangered freshwater fish Zingel asper (the Rhone streber). We conducted modelling and simulation analyses to identify and characterize some of the drivers of individual trophic trait variation in this species. We found that population density and ontogeny had minor effects on the trophic niche of Z. asper. Instead, our results suggest that the majority of trophic niche variation was driven by seasonal variation in ecological opportunity. The total trophic niche width of Z. asper seasonally expanded to include a broader range of prey. Furthermore, null model simulations revealed that the increase of between‐individual variation in autumn indicates that Z. asper become more opportunistic relative to summer and spring, rather than being associated with a seasonal...
Fastq file with raw data of Illumina reads Plate: DNA9 Marker: MFZR Tag combinations to identify ... more Fastq file with raw data of Illumina reads Plate: DNA9 Marker: MFZR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
Fastq file with raw data of Illumina reads Plate: DNA25 Marker: LFCR Tag combinations to identify... more Fastq file with raw data of Illumina reads Plate: DNA25 Marker: LFCR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
Fastq file with raw data of Illumina reads Plate: DNA24 Marker: MFZR Tag combinations to identify... more Fastq file with raw data of Illumina reads Plate: DNA24 Marker: MFZR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
Fastq file with raw data of Illumina reads Plate: DNA23 Marker: ZFZR Tag combinations to identify... more Fastq file with raw data of Illumina reads Plate: DNA23 Marker: ZFZR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
Fastq file with raw data of Illumina reads Plate: DNA23 Marker: LFCR Tag combinations to identify... more Fastq file with raw data of Illumina reads Plate: DNA23 Marker: LFCR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
HAL (Le Centre pour la Communication Scientifique Directe), Jun 14, 2023
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Ten novel polymorphic microsatellites (seven with perfect motifs) were isolated from vairone spec... more Ten novel polymorphic microsatellites (seven with perfect motifs) were isolated from vairone species (Telestes souffia and Telestes muticellus), which are endangered European cyprinid species. Together with 11 previously published cyprinid-specific loci, five multiplex sets were optimized, allowing the genotyping of 21 polymorphic loci. The level of genetic diversity was assessed in 97 individuals from the two species T. souffia and T. muticellus. We also successfully applied the 21 microsatellites to nine related species. These primers will thus be useful in assessing population structure of the vairone and other cyprinid species, with application for conservation issues and phylogeographical approaches.
Ten novel polymorphic microsatellites were isolated from the dace complex (Leuciscus leuciscus), ... more Ten novel polymorphic microsatellites were isolated from the dace complex (Leuciscus leuciscus), which is a European cyprinid species. Four multiplex polymerase chain reaction sets were optimized in order to genotype 26 polymorphic loci in all, including 16 previously published cyprinid-specific loci. The level of genetic diversity was assessed in 142 dace individuals. We also successfully applied 26 of the microsatellites to 10 related species. These primers thus will be useful to assess population structure of the dace and other cyprinid species, with application for conservation issues and phylogeographical approaches.
Aquatic Conservation: Marine and Freshwater Ecosystems
Intraspecific variation in life histories and its environmental correlates can indicate the degre... more Intraspecific variation in life histories and its environmental correlates can indicate the degree of vulnerability to extinction of endangered taxa and guide conservation actions. Zingel asper (Percidae) is an endangered fish endemic to the Rhône catchment (France and Switzerland), where five populations subsist in separate river systems (Loue, Beaume, Durance, Verdon, and Doubs). Two populations of Z. asper differ in growth and longevity, but the existence of broader intraspecific differentiation in life histories and the environmental origins of this variation (if any) remain unknown. The age structure and growth profile of four populations of Z. asper (Loue, Beaume, Durance, and Verdon) and nine additional sub‐populations within the Durance system were determined by scale‐reading analysis, before evaluating the contribution of measured variation in substrate quality, hydraulics, prey availability, and water temperature to growth differentiation among and within populations. A tr...
Le peuplement et la mise en place de la population de l'ile de La Reunion, a l'instar des... more Le peuplement et la mise en place de la population de l'ile de La Reunion, a l'instar des Antilles ou de la Guyane francaises, sont intimement lies aux apports issus de la Traite des esclaves. Alors que les populations, concernees par la Traite atlantique furent en grande majorite originaires de l'Afrique de l'Ouest, les donnees historiques concernant La Reunion montrent clairement que ce fut la cote Est Africaine et Madagascar qui furent les regions les plus sollicitees. Cependant, certaines zones d'ombre persistent concernant l'origine geographique des esclaves africains importes a La Reunion, mais aussi les contributions respectives de Madagascar et de l'Afrique subsaharienne au pool afro-malgache reunionnais. A travers l'analyse des polymorphismes de l'ADN mitochondrial d'un echantillon de la Population reunionnaise, nous apportons ici des elements de reponse a ces deux questions. Nos analyses ont aussi permis d'explorer la dynamique t...
Dietary studies are critical for understanding foraging strategies and have important application... more Dietary studies are critical for understanding foraging strategies and have important applications in conservation and habitat management. We applied a robust metabarcoding protocol to characterize the diet of the critically endangered freshwater fish Zingel asper (the Rhone streber). We conducted modelling and simulation analyses to identify and characterize some of the drivers of individual trophic trait variation in this species. We found that population density and ontogeny had minor effects on the trophic niche of Z. asper. Instead, our results suggest that the majority of trophic niche variation was driven by seasonal variation in ecological opportunity. The total trophic niche width of Z. asper seasonally expanded to include a broader range of prey. Furthermore, null model simulations revealed that the increase of between‐individual variation in autumn indicates that Z. asper become more opportunistic relative to summer and spring, rather than being associated with a seasonal...
Fastq file with raw data of Illumina reads Plate: DNA9 Marker: MFZR Tag combinations to identify ... more Fastq file with raw data of Illumina reads Plate: DNA9 Marker: MFZR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
Fastq file with raw data of Illumina reads Plate: DNA25 Marker: LFCR Tag combinations to identify... more Fastq file with raw data of Illumina reads Plate: DNA25 Marker: LFCR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
Fastq file with raw data of Illumina reads Plate: DNA24 Marker: MFZR Tag combinations to identify... more Fastq file with raw data of Illumina reads Plate: DNA24 Marker: MFZR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
Fastq file with raw data of Illumina reads Plate: DNA23 Marker: ZFZR Tag combinations to identify... more Fastq file with raw data of Illumina reads Plate: DNA23 Marker: ZFZR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
Fastq file with raw data of Illumina reads Plate: DNA23 Marker: LFCR Tag combinations to identify... more Fastq file with raw data of Illumina reads Plate: DNA23 Marker: LFCR Tag combinations to identify the samples from each fastq files are found in the 'CSV file with metadata on fastq files
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